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The map label for this gene is mnmE [H]

Identifier: 238926048

GI number: 238926048

Start: 3445039

End: 3446415

Strand: Reverse

Name: mnmE [H]

Synonym: EUBREC_3707

Alternate gene names: 238926048

Gene position: 3446415-3445039 (Counterclockwise)

Preceding gene: 238926049

Following gene: 238926047

Centisome position: 99.91

GC content: 39.22

Gene sequence:

>1377_bases
ATGCAAAATGATACAATAGCGGCTATTGCCACAGCTATGTCTCCGTCAGGAATCGGAATTATCAGGATAAGCGGAGATGA
TGCACTTTCGGTAATAGATAGGATTTATAAAAGCAAGAATAATAAAAAGAAAATAAGCGCCTGTCAGTCACATACCATTC
ATTATGGTTTTATATATGACGGCGATGAGAAAATAGATGAGGTTATGGTGCTTTTGATGAAGGCTCCAAATACATACACC
AGGGAGGACACTGTGGAGATAGACTGCCACGGTGGTGTGTATGTTATGAAGAGAGTGCTTGAAGCGGTAATCAAAAACGG
AGCAAGACCGGCAGAGCCCGGAGAGTTTACTAAAAGGGCATTTTTAAACGGCAGGATTGATTTATCCCAGGCAGAATCTG
TTATAGATGTAATAAATTCAAAAAATGATTTTGCGCTCAAAAGCTCTTTAAGCCAGCTAGGCGGAGCCGTGCTCGGAAGC
ATCAGGCAGATAAGAGAGCAGCTTTTGCATGAGATTGCTTTTATTGAGTCGGCACTTGACGACCCTGAACACATTTCCCT
GGATGGTTATCCACAAAAACTACGTGCAATTGTGGATAACGAGTATATGGAGATAGATGGTCTGTTAAAAACCTCAGATA
ATGGCAGGATTCTGAAGGAAGGAATAAATACAGTCATCATTGGAAAGCCAAATGCCGGAAAGTCATCTCTTTTAAATGTG
CTTGTCGGAACTGACAGGGCAATTGTCACGGATATAGCCGGAACCACGAGAGATGTGCTTGAGGAGCAGATTAATATTGG
TGGAATAACCTTAAATCTCGTTGATACTGCAGGTATTAGAAGCACTGAGGATGTTGTTGAAAAAATTGGTGTGAAAAAGG
CAATGGAGCATGCCAATGGAGCTGATTTAATCATATATGTGGTTGATTCGTCAGTGGCACTTGATGATAATGATTATGAT
ATTATCAATTTTATTAAGGATAAAAAGGCTGTCATTTTACTAAATAAGTCGGATCTGGCTTCAAAGGTGTCGGCAGATGA
TATAAAAAAGCTTGTGGATAAAACGGTTATTTCGGTTTCTGCAAAGGAAAGCTCGGGAATAGATGAGCTTTCTGATACAA
TAAAGGAAATGTTTTTTGATGGAGAGGTCTCATTCAATGATGAGATTTATATCACAAATATCAGGCATAAAAAACTATTG
TCAGATGCTAAGGAAAGTCTTAAGCTTGTTATGAACAGTATTGATGATGATATGCCGGAGGATTTTTATTCGATTGATTT
GATGAGTGCGTATGAGTCGCTTGGACTCATAATAGGAGAATCGGTTGAGGATGATCTGATGGATGAGATATTCAGTAAAT
TTTGTATGGGAAAGTAG

Upstream 100 bases:

>100_bases
TGACACTTAAGAAATAATAGATATAAATCATTAAAAGGGTATCTGGTTTCAGGTATCCTTTTTGAATATATATTATCTTA
GAATTTTTGAGGAATTTCAT

Downstream 100 bases:

>100_bases
GCAGTTATGTGCAGTAAGTTTGTTGAAGAAAATTATGATGTTGTTGTAGTTGGTGCAGGACATGCGGGATGTGAGGCAGC
ACTTGCGTGTGCCAGGCTTG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKKISACQSHTIHYGFIYDGDEKIDEVMVLLMKAPNTYT
REDTVEIDCHGGVYVMKRVLEAVIKNGARPAEPGEFTKRAFLNGRIDLSQAESVIDVINSKNDFALKSSLSQLGGAVLGS
IRQIREQLLHEIAFIESALDDPEHISLDGYPQKLRAIVDNEYMEIDGLLKTSDNGRILKEGINTVIIGKPNAGKSSLLNV
LVGTDRAIVTDIAGTTRDVLEEQINIGGITLNLVDTAGIRSTEDVVEKIGVKKAMEHANGADLIIYVVDSSVALDDNDYD
IINFIKDKKAVILLNKSDLASKVSADDIKKLVDKTVISVSAKESSGIDELSDTIKEMFFDGEVSFNDEIYITNIRHKKLL
SDAKESLKLVMNSIDDDMPEDFYSIDLMSAYESLGLIIGESVEDDLMDEIFSKFCMGK

Sequences:

>Translated_458_residues
MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKKISACQSHTIHYGFIYDGDEKIDEVMVLLMKAPNTYT
REDTVEIDCHGGVYVMKRVLEAVIKNGARPAEPGEFTKRAFLNGRIDLSQAESVIDVINSKNDFALKSSLSQLGGAVLGS
IRQIREQLLHEIAFIESALDDPEHISLDGYPQKLRAIVDNEYMEIDGLLKTSDNGRILKEGINTVIIGKPNAGKSSLLNV
LVGTDRAIVTDIAGTTRDVLEEQINIGGITLNLVDTAGIRSTEDVVEKIGVKKAMEHANGADLIIYVVDSSVALDDNDYD
IINFIKDKKAVILLNKSDLASKVSADDIKKLVDKTVISVSAKESSGIDELSDTIKEMFFDGEVSFNDEIYITNIRHKKLL
SDAKESLKLVMNSIDDDMPEDFYSIDLMSAYESLGLIIGESVEDDLMDEIFSKFCMGK
>Mature_458_residues
MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKKISACQSHTIHYGFIYDGDEKIDEVMVLLMKAPNTYT
REDTVEIDCHGGVYVMKRVLEAVIKNGARPAEPGEFTKRAFLNGRIDLSQAESVIDVINSKNDFALKSSLSQLGGAVLGS
IRQIREQLLHEIAFIESALDDPEHISLDGYPQKLRAIVDNEYMEIDGLLKTSDNGRILKEGINTVIIGKPNAGKSSLLNV
LVGTDRAIVTDIAGTTRDVLEEQINIGGITLNLVDTAGIRSTEDVVEKIGVKKAMEHANGADLIIYVVDSSVALDDNDYD
IINFIKDKKAVILLNKSDLASKVSADDIKKLVDKTVISVSAKESSGIDELSDTIKEMFFDGEVSFNDEIYITNIRHKKLL
SDAKESLKLVMNSIDDDMPEDFYSIDLMSAYESLGLIIGESVEDDLMDEIFSKFCMGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=478, Percent_Identity=29.4979079497908, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI193082993, Length=464, Percent_Identity=29.7413793103448, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI193082988, Length=508, Percent_Identity=28.740157480315, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI2367268, Length=467, Percent_Identity=35.117773019272, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI87082120, Length=170, Percent_Identity=31.7647058823529, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=31.8279569892473, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323665, Length=508, Percent_Identity=30.1181102362205, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=29.1666666666667, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50271; Mature: 50271

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKKISACQSHTIHYGFIYD
CCCCCEEHHEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEC
GDEKIDEVMVLLMKAPNTYTREDTVEIDCHGGVYVMKRVLEAVIKNGARPAEPGEFTKRA
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLSQAESVIDVINSKNDFALKSSLSQLGGAVLGSIRQIREQLLHEIAFIESALD
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPEHISLDGYPQKLRAIVDNEYMEIDGLLKTSDNGRILKEGINTVIIGKPNAGKSSLLNV
CCCEECCCCCHHHHHHHHCCCCEEECCEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHH
LVGTDRAIVTDIAGTTRDVLEEQINIGGITLNLVDTAGIRSTEDVVEKIGVKKAMEHANG
HHCCCCCEEHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ADLIIYVVDSSVALDDNDYDIINFIKDKKAVILLNKSDLASKVSADDIKKLVDKTVISVS
CEEEEEEECCCEEECCCCCHHHHHHCCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHEEE
AKESSGIDELSDTIKEMFFDGEVSFNDEIYITNIRHKKLLSDAKESLKLVMNSIDDDMPE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
DFYSIDLMSAYESLGLIIGESVEDDLMDEIFSKFCMGK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNDTIAAIATAMSPSGIGIIRISGDDALSVIDRIYKSKNNKKKISACQSHTIHYGFIYD
CCCCCEEHHEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEC
GDEKIDEVMVLLMKAPNTYTREDTVEIDCHGGVYVMKRVLEAVIKNGARPAEPGEFTKRA
CHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
FLNGRIDLSQAESVIDVINSKNDFALKSSLSQLGGAVLGSIRQIREQLLHEIAFIESALD
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPEHISLDGYPQKLRAIVDNEYMEIDGLLKTSDNGRILKEGINTVIIGKPNAGKSSLLNV
CCCEECCCCCHHHHHHHHCCCCEEECCEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHH
LVGTDRAIVTDIAGTTRDVLEEQINIGGITLNLVDTAGIRSTEDVVEKIGVKKAMEHANG
HHCCCCCEEHHHCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ADLIIYVVDSSVALDDNDYDIINFIKDKKAVILLNKSDLASKVSADDIKKLVDKTVISVS
CEEEEEEECCCEEECCCCCHHHHHHCCCCEEEEEECHHHHHHCCHHHHHHHHHHHHHEEE
AKESSGIDELSDTIKEMFFDGEVSFNDEIYITNIRHKKLLSDAKESLKLVMNSIDDDMPE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
DFYSIDLMSAYESLGLIIGESVEDDLMDEIFSKFCMGK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA