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The map label for this gene is dacD [C]

Identifier: 238925895

GI number: 238925895

Start: 3315614

End: 3316570

Strand: Reverse

Name: dacD [C]

Synonym: EUBREC_3553

Alternate gene names: 238925895

Gene position: 3316570-3315614 (Counterclockwise)

Preceding gene: 238925896

Following gene: 238925894

Centisome position: 96.14

GC content: 39.18

Gene sequence:

>957_bases
ATGCAGAGAAAACGCCGTAAGAAAAAACGATTTGGAGTATTTTTGTTTTTGCTTGTAGTTTGTATCGGCATAGGTATAAT
GTTTGTGCCGTGGGCAATGCAGCCGGAAAATTTCAGAGAGGTAAAGAATAAAATAAATGGTTTTCTCTACAAAGAAGATT
TTCCTGACTCTTATAATGCAAAATCGCTGATTTTGGTGGATTGTTCTGATGATGAAATATTTGTTTCAAAAAACGAGAAC
GAGCCACAAATTCCTGCAAGTTTAGCCAAGCTGTTTGTTATTGAGTATGCGTCAACTTTAGCAGACCTTGATAGTATCGT
TGTTGCAGACTATGGTGCTATTTCACTCACGAAACCTGGCTCGTCTGTTGCTCAAATCAAGGAAAAAGAATACTTTTTAC
ACAATCTATTTGCGGCAATGTTAGTACCGTCAGGGAATGACGCAGCTTATGTGGTTGCTGATTATTGTGGAGGATTACTT
TCGCCGCAAGCAGAAAGCTGTCAAGAGCGTGTGAGGATTTTCATGGAGAACTTAAACCTGCATTTGCAACAGCAGGGGTA
CTTAGACACAGTTTTATATGATCCGAGTGGTTTTGATATGGAGGCACTTACCACCACTTTAGATTTGAAAGAAGTAGTAT
ATCGGTTGTTGGAATACTCGTGGTTTAGAGAAATTGTATCTCAAAACACTTATACGGCAACACTGCCTGATGGCAGTACA
CAAATATGGCAGAATACAAACGCCTTCCTTGATCCGACATCCGAATATTACAACGAAAATGTATGCGGAATTAAGACTGG
CTCTCTGTCTGACGACTATAACTTGATTGTGCTTTATCAGCAGCACGGAAAAGAGTTTTTGATATGCAGTTTAGGCTCTC
AGTCCGATTCCTCTCGTTATGACGATGTGAATTTTATTCTGAAAACGATTGATGAATCGTATTATCTCACGCAATAA

Upstream 100 bases:

>100_bases
AAGTTGGGAGATCCGTCTGGATTTACAGAGTGTATTCAGGCAAATTATGCCGATTAAAAATGGCACATAGGAGTTTTGGT
GTTGGGAGGTGAGCACATAT

Downstream 100 bases:

>100_bases
ATCTTATGTTGTCCAAGACAAAAAAACTAACCAAGGGGGGTGATAATATCGGACGAATCGTAATAATGGAGTTCAAAGCA
CAAGACTCAACAGCCTTTGA

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: 2 D-Alanine. [C]

Alternate protein names: Serine-Type D-Ala-D-Ala Carboxypeptidase; Peptidase; Beta-Lactamase; D-Alanyl-D-Alanine Carboxypeptidase Family Protein; D D Carboxypeptidase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MQRKRRKKKRFGVFLFLLVVCIGIGIMFVPWAMQPENFREVKNKINGFLYKEDFPDSYNAKSLILVDCSDDEIFVSKNEN
EPQIPASLAKLFVIEYASTLADLDSIVVADYGAISLTKPGSSVAQIKEKEYFLHNLFAAMLVPSGNDAAYVVADYCGGLL
SPQAESCQERVRIFMENLNLHLQQQGYLDTVLYDPSGFDMEALTTTLDLKEVVYRLLEYSWFREIVSQNTYTATLPDGST
QIWQNTNAFLDPTSEYYNENVCGIKTGSLSDDYNLIVLYQQHGKEFLICSLGSQSDSSRYDDVNFILKTIDESYYLTQ

Sequences:

>Translated_318_residues
MQRKRRKKKRFGVFLFLLVVCIGIGIMFVPWAMQPENFREVKNKINGFLYKEDFPDSYNAKSLILVDCSDDEIFVSKNEN
EPQIPASLAKLFVIEYASTLADLDSIVVADYGAISLTKPGSSVAQIKEKEYFLHNLFAAMLVPSGNDAAYVVADYCGGLL
SPQAESCQERVRIFMENLNLHLQQQGYLDTVLYDPSGFDMEALTTTLDLKEVVYRLLEYSWFREIVSQNTYTATLPDGST
QIWQNTNAFLDPTSEYYNENVCGIKTGSLSDDYNLIVLYQQHGKEFLICSLGSQSDSSRYDDVNFILKTIDESYYLTQ
>Mature_318_residues
MQRKRRKKKRFGVFLFLLVVCIGIGIMFVPWAMQPENFREVKNKINGFLYKEDFPDSYNAKSLILVDCSDDEIFVSKNEN
EPQIPASLAKLFVIEYASTLADLDSIVVADYGAISLTKPGSSVAQIKEKEYFLHNLFAAMLVPSGNDAAYVVADYCGGLL
SPQAESCQERVRIFMENLNLHLQQQGYLDTVLYDPSGFDMEALTTTLDLKEVVYRLLEYSWFREIVSQNTYTATLPDGST
QIWQNTNAFLDPTSEYYNENVCGIKTGSLSDDYNLIVLYQQHGKEFLICSLGSQSDSSRYDDVNFILKTIDESYYLTQ

Specific function: Removes C-Terminal D-Alanyl Residues From Sugar-Peptide Cell Wall Precursors. [C]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Inner Membrane-Associated. N-Terminal Lies In The Periplasmic Protein Space [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082022, Length=278, Percent_Identity=27.3381294964029, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4 [C]

Molecular weight: Translated: 36174; Mature: 36174

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKRRKKKRFGVFLFLLVVCIGIGIMFVPWAMQPENFREVKNKINGFLYKEDFPDSYNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCC
KSLILVDCSDDEIFVSKNENEPQIPASLAKLFVIEYASTLADLDSIVVADYGAISLTKPG
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCC
SSVAQIKEKEYFLHNLFAAMLVPSGNDAAYVVADYCGGLLSPQAESCQERVRIFMENLNL
CHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCE
HLQQQGYLDTVLYDPSGFDMEALTTTLDLKEVVYRLLEYSWFREIVSQNTYTATLPDGST
EEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
QIWQNTNAFLDPTSEYYNENVCGIKTGSLSDDYNLIVLYQQHGKEFLICSLGSQSDSSRY
HHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCC
DDVNFILKTIDESYYLTQ
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQRKRRKKKRFGVFLFLLVVCIGIGIMFVPWAMQPENFREVKNKINGFLYKEDFPDSYNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCC
KSLILVDCSDDEIFVSKNENEPQIPASLAKLFVIEYASTLADLDSIVVADYGAISLTKPG
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCC
SSVAQIKEKEYFLHNLFAAMLVPSGNDAAYVVADYCGGLLSPQAESCQERVRIFMENLNL
CHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCE
HLQQQGYLDTVLYDPSGFDMEALTTTLDLKEVVYRLLEYSWFREIVSQNTYTATLPDGST
EEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
QIWQNTNAFLDPTSEYYNENVCGIKTGSLSDDYNLIVLYQQHGKEFLICSLGSQSDSSRY
HHHCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCC
DDVNFILKTIDESYYLTQ
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Alanyl-D-Alanine; H2O [C]

Specific reaction: D-Alanyl-D-Alanine + H2O = 2 D-Alanine. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA