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The map label for this gene is mazF [H]

Identifier: 238925873

GI number: 238925873

Start: 3302633

End: 3303013

Strand: Reverse

Name: mazF [H]

Synonym: EUBREC_3531

Alternate gene names: 238925873

Gene position: 3303013-3302633 (Counterclockwise)

Preceding gene: 238925874

Following gene: 238925872

Centisome position: 95.75

GC content: 42.78

Gene sequence:

>381_bases
GTGACGATTCGAAGAGGAGATATCTTATGGGCGGATCTCGGTATGTTTCCGACAACTTCGGTTCAGGGTGGAGTAAGACC
GGTGATCGTAGTAAGTAACAACAAAGCTAATACATACAGTTCAGTCATTACAGTAGTTCCGCTGACGTCAAGAATATATA
AGAAGCGGTATTTGCCAACACATGTATTTATCAGTAAGTACGATATGACAGGGATCCGAAAAGGAAGCCTGGCATTGGCT
GAACAGGTTATGAGTATTTCTACAAAATGTATTATTGAGAAATGCGGAAGAGTAAATAAGTGGAGCCTGGATCGGGTACT
GAAAGCAGTACGGATTCAGATGGGAATGGAAGGAGAAGGGCATGACAGCAGAGGAATATAG

Upstream 100 bases:

>100_bases
CAGAGCTTTCCATCTTGGAAAGGAACTCTGCCGTATTCCTGTGGAGCCAATATAGCGCTAGGGATGCTTTACAAATAAAA
AAAGATTGGAGAGATGACAA

Downstream 100 bases:

>100_bases
AAATTATCTGGATCAGGATTTCAGTGATGTGGATATCAATGAAATGACTGATCTACGGATGATAAAAACAGACAGAAATA
AATCGCTTCAGGAACGGAGA

Product: PemK family DNA-binding protein

Products: NA

Alternate protein names: Endoribonuclease MazF; Toxin MazF [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MTIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVITVVPLTSRIYKKRYLPTHVFISKYDMTGIRKGSLALA
EQVMSISTKCIIEKCGRVNKWSLDRVLKAVRIQMGMEGEGHDSRGI

Sequences:

>Translated_126_residues
MTIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVITVVPLTSRIYKKRYLPTHVFISKYDMTGIRKGSLALA
EQVMSISTKCIIEKCGRVNKWSLDRVLKAVRIQMGMEGEGHDSRGI
>Mature_125_residues
TIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVITVVPLTSRIYKKRYLPTHVFISKYDMTGIRKGSLALAE
QVMSISTKCIIEKCGRVNKWSLDRVLKAVRIQMGMEGEGHDSRGI

Specific function: Part of a toxin-antitoxin (TA) module. Ribosome- independent, sequence-specific endoribonuclease that cleaves mRNA, thus inhibiting protein synthesis and inducing bacterial stasis. Cleavages occur preferentially in a U-rich region with a consensus sequenc

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 14045; Mature: 13914

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVITVVPLTSRIYKKRYLPT
CCCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHHHEEEEEEHHHHHHHHHCCCC
HVFISKYDMTGIRKGSLALAEQVMSISTKCIIEKCGRVNKWSLDRVLKAVRIQMGMEGEG
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
HDSRGI
CCCCCC
>Mature Secondary Structure 
TIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVITVVPLTSRIYKKRYLPT
CCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCHHHEEEEEEHHHHHHHHHCCCC
HVFISKYDMTGIRKGSLALAEQVMSISTKCIIEKCGRVNKWSLDRVLKAVRIQMGMEGEG
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
HDSRGI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA