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The map label for this gene is def [H]

Identifier: 238925766

GI number: 238925766

Start: 3224991

End: 3225398

Strand: Reverse

Name: def [H]

Synonym: EUBREC_3423

Alternate gene names: 238925766

Gene position: 3225398-3224991 (Counterclockwise)

Preceding gene: 238925767

Following gene: 238925765

Centisome position: 93.5

GC content: 41.42

Gene sequence:

>408_bases
ATGGTAAAGAAGATAATGCGTGATCCACTGTTTCTTGCACAGAAATCAGAGGATGCTACAGAAGATGATAAACAGGTGGT
TACGGATTTACTTGACACATTGAAAGCAAATCTTGACCATTGTGTTGGAATGGCAGCTAACATGATTGGTGTGAAAAAGA
ATATCATAGTGGTTGCGGCCGGACCATTCCAGTTTGCCATGATTAATCCGGTGATTACCGGAAAATCAGGAGCTTTTCAG
ACGGAGGAAGGCTGTCTTTCGCTGGAGGGTGTGAGACCATGCACAAGATATAAAGAGATTGAAGTGGATTATCTAGACCA
GAATTTCAAAAAGAAACATGGCAAATACACAGGCTGGACAGCACAGATAATTCAGCATGATGAATGCGATATAATAGGGA
CAAGTTAG

Upstream 100 bases:

>100_bases
GATAGATGAAAGGCTAAATATGAAAAATGAAATTTTATTAATTAATGTACTTATTGAGACATATATGGTGGAAATATACC
TGGACAGAAGGGAGTAAATA

Downstream 100 bases:

>100_bases
AAGAAAACCTTGAAAACAAGGGGTTCACGCTGATTTAGTCCCTATTTTTTATACCATTTAATCGGTAGATAAGGCAGCGC
TGTTATTTAAAATCCGAAAT

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MVKKIMRDPLFLAQKSEDATEDDKQVVTDLLDTLKANLDHCVGMAANMIGVKKNIIVVAAGPFQFAMINPVITGKSGAFQ
TEEGCLSLEGVRPCTRYKEIEVDYLDQNFKKKHGKYTGWTAQIIQHDECDIIGTS

Sequences:

>Translated_135_residues
MVKKIMRDPLFLAQKSEDATEDDKQVVTDLLDTLKANLDHCVGMAANMIGVKKNIIVVAAGPFQFAMINPVITGKSGAFQ
TEEGCLSLEGVRPCTRYKEIEVDYLDQNFKKKHGKYTGWTAQIIQHDECDIIGTS
>Mature_135_residues
MVKKIMRDPLFLAQKSEDATEDDKQVVTDLLDTLKANLDHCVGMAANMIGVKKNIIVVAAGPFQFAMINPVITGKSGAFQ
TEEGCLSLEGVRPCTRYKEIEVDYLDQNFKKKHGKYTGWTAQIIQHDECDIIGTS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 14993; Mature: 14993

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKIMRDPLFLAQKSEDATEDDKQVVTDLLDTLKANLDHCVGMAANMIGVKKNIIVVAA
CCHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GPFQFAMINPVITGKSGAFQTEEGCLSLEGVRPCTRYKEIEVDYLDQNFKKKHGKYTGWT
CCEEEEEECEEEECCCCCCCCCCCCEECCCCCCCCHHHCCCHHHHCCHHHHHCCCCCCCE
AQIIQHDECDIIGTS
EEEEECCCCCEEECC
>Mature Secondary Structure
MVKKIMRDPLFLAQKSEDATEDDKQVVTDLLDTLKANLDHCVGMAANMIGVKKNIIVVAA
CCHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GPFQFAMINPVITGKSGAFQTEEGCLSLEGVRPCTRYKEIEVDYLDQNFKKKHGKYTGWT
CCEEEEEECEEEECCCCCCCCCCCCEECCCCCCCCHHHCCCHHHHCCHHHHHCCCCCCCE
AQIIQHDECDIIGTS
EEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9572951 [H]