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The map label for this gene is 238925683

Identifier: 238925683

GI number: 238925683

Start: 3161744

End: 3162634

Strand: Reverse

Name: 238925683

Synonym: EUBREC_3340

Alternate gene names: NA

Gene position: 3162634-3161744 (Counterclockwise)

Preceding gene: 238925684

Following gene: 238925680

Centisome position: 91.68

GC content: 48.37

Gene sequence:

>891_bases
ATGAGAAAGGTGAATAGTATGAGAGAGGTTACAATTGCTGCAATCCAGATGAGCTGCAGCCGTGACGTAAAAGAAAATAT
TGAAAAAGCAGCAAAGCTTATAAGGGCTGCGGCAGAGGCAGGAGCACAGATAATACTTCCTTCGGAGCTATTTGAGAGAC
AGTATTTCTGTCAGGAGAGGCGCTACGATTATTATGACTATGCAAAGCCGCTTTCTGAAAATGATGCGGTGCAGAGCATG
AAGGCACTTGCAAAGGAGCTTGGCGTAGTGATTCCTGTGAGCTTTTATGAGGCGGGAGAGGGCAGACAGCTTTTCAACTC
AGTGGCGGTGATAGATGCCGATGGAGAGGTGCTTGGAATATACCGAAAGACACATATCCCGGACGATCATTATTATCAGG
AGAAGTTTTACTTCACACCGGGAAATACAGGCTTTAAAGCTTTTAAGACAAGATATGCAACAATTGGAGTCGGCATATGC
TGGGATCAGTGGTTCCCGGAAACGGCAAGAGGCATGGCTTTAAAGGGCGCGGAGATACTTTTTTACCCTACAGCCATAGG
CAGCGAGCCGATACTGGAATGTGACAGCATGCCACACTGGAGAAGGTGTATGACCGGACATGCAGCCTGCAATCTGATGC
CGGTAGTGGCGGCAAACAGGATAGGCACAGAGGAGGTTGTACCGTGTGCAGAAAACGGAAACCAGAGCTCTGCACTGACA
TTTTATGGTTCTTCGTTTATCACAGACGCAACCGGAGAGGTTGTTGAGCAGATGGACAGAGTATCGGAGGGCTTCATACT
CCACAGTTTTGATTTGGATGAGCTGGAGAGTGAGAGAAAGAGCTGGGGGCTGTTCAGGGACAGGCGTCCGGAGATGTATG
GGGAGAATTAG

Upstream 100 bases:

>100_bases
AGGGAAGAAAAGTGTATCCGATATATGCGCGCGACATAATTGTCGGCGGGGGAAATATACACTGTATCACACAGCAGATA
CCGCAGTAATTTAATTCAGG

Downstream 100 bases:

>100_bases
CTTAGATTACCCTAATGATTGCAAATTAATTTAAAGTTAATTTAAAACACAGGATACAGCAAAGGCGCTATATCCTGTGT
TTTTTATAGTTCCATATTAG

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRKVNSMREVTIAAIQMSCSRDVKENIEKAAKLIRAAAEAGAQIILPSELFERQYFCQERRYDYYDYAKPLSENDAVQSM
KALAKELGVVIPVSFYEAGEGRQLFNSVAVIDADGEVLGIYRKTHIPDDHYYQEKFYFTPGNTGFKAFKTRYATIGVGIC
WDQWFPETARGMALKGAEILFYPTAIGSEPILECDSMPHWRRCMTGHAACNLMPVVAANRIGTEEVVPCAENGNQSSALT
FYGSSFITDATGEVVEQMDRVSEGFILHSFDLDELESERKSWGLFRDRRPEMYGEN

Sequences:

>Translated_296_residues
MRKVNSMREVTIAAIQMSCSRDVKENIEKAAKLIRAAAEAGAQIILPSELFERQYFCQERRYDYYDYAKPLSENDAVQSM
KALAKELGVVIPVSFYEAGEGRQLFNSVAVIDADGEVLGIYRKTHIPDDHYYQEKFYFTPGNTGFKAFKTRYATIGVGIC
WDQWFPETARGMALKGAEILFYPTAIGSEPILECDSMPHWRRCMTGHAACNLMPVVAANRIGTEEVVPCAENGNQSSALT
FYGSSFITDATGEVVEQMDRVSEGFILHSFDLDELESERKSWGLFRDRRPEMYGEN
>Mature_296_residues
MRKVNSMREVTIAAIQMSCSRDVKENIEKAAKLIRAAAEAGAQIILPSELFERQYFCQERRYDYYDYAKPLSENDAVQSM
KALAKELGVVIPVSFYEAGEGRQLFNSVAVIDADGEVLGIYRKTHIPDDHYYQEKFYFTPGNTGFKAFKTRYATIGVGIC
WDQWFPETARGMALKGAEILFYPTAIGSEPILECDSMPHWRRCMTGHAACNLMPVVAANRIGTEEVVPCAENGNQSSALT
FYGSSFITDATGEVVEQMDRVSEGFILHSFDLDELESERKSWGLFRDRRPEMYGEN

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=295, Percent_Identity=29.1525423728814, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI297632350, Length=295, Percent_Identity=29.1525423728814, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI5031947, Length=295, Percent_Identity=29.1525423728814, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI9910460, Length=295, Percent_Identity=28.135593220339, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7706509, Length=292, Percent_Identity=27.7397260273973, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI297632346, Length=187, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=303, Percent_Identity=29.7029702970297, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17556280, Length=291, Percent_Identity=24.7422680412371, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=298, Percent_Identity=24.8322147651007, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6322335, Length=306, Percent_Identity=23.8562091503268, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358471, Length=307, Percent_Identity=29.6416938110749, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21355835, Length=299, Percent_Identity=25.0836120401338, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17933642, Length=289, Percent_Identity=25.2595155709343, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33424; Mature: 33424

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVNSMREVTIAAIQMSCSRDVKENIEKAAKLIRAAAEAGAQIILPSELFERQYFCQER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
RYDYYDYAKPLSENDAVQSMKALAKELGVVIPVSFYEAGEGRQLFNSVAVIDADGEVLGI
CCCHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEHCCCCCCHHHHHCEEEEECCCCEEEE
YRKTHIPDDHYYQEKFYFTPGNTGFKAFKTRYATIGVGICWDQWFPETARGMALKGAEIL
EEECCCCCCCEECCEEEECCCCCCHHHHHHHHEEEEEEEHHHHCCCHHHCCCEECCCEEE
FYPTAIGSEPILECDSMPHWRRCMTGHAACNLMPVVAANRIGTEEVVPCAENGNQSSALT
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCEEHHHHHCCCCCHHCCCCCCCCCCCCEEE
FYGSSFITDATGEVVEQMDRVSEGFILHSFDLDELESERKSWGLFRDRRPEMYGEN
EECCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHCCCCCCCCCC
>Mature Secondary Structure
MRKVNSMREVTIAAIQMSCSRDVKENIEKAAKLIRAAAEAGAQIILPSELFERQYFCQER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
RYDYYDYAKPLSENDAVQSMKALAKELGVVIPVSFYEAGEGRQLFNSVAVIDADGEVLGI
CCCHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEHCCCCCCHHHHHCEEEEECCCCEEEE
YRKTHIPDDHYYQEKFYFTPGNTGFKAFKTRYATIGVGICWDQWFPETARGMALKGAEIL
EEECCCCCCCEECCEEEECCCCCCHHHHHHHHEEEEEEEHHHHCCCHHHCCCEECCCEEE
FYPTAIGSEPILECDSMPHWRRCMTGHAACNLMPVVAANRIGTEEVVPCAENGNQSSALT
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCEEHHHHHCCCCCHHCCCCCCCCCCCCEEE
FYGSSFITDATGEVVEQMDRVSEGFILHSFDLDELESERKSWGLFRDRRPEMYGEN
EECCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]