BacMap

Definition	Eubacterium rectale ATCC 33656, complete genome.
Accession	NC_012781
Length	3,449,685

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The map label for this gene is dnaJ [H]

Identifier: 238925200

GI number: 238925200

Start: 2734787

End: 2735857

Strand: Reverse

Name: dnaJ [H]

Synonym: EUBREC_2854

Alternate gene names: 238925200

Gene position: 2735857-2734787 (Counterclockwise)

Preceding gene: 238925201

Following gene: 238925199

Centisome position: 79.31

GC content: 50.23

Gene sequence:

>1071_bases
ATGGCAGCAAAGAGAGATTATTATGAGACTCTCGGAGTGAACAAGAGTGCGGATAAAGAGGCAATAAAGAGGGCGTATCG
TAAGCTGGCAAAGAAGTATCATCCTGATACAAATGCAGGCAATCCGCACGCTGAGGAGATGTTTAAGGATGTCACGGAGG
CATACAATGTATTAAGTGATGAGAAAAAACGTAAGCTTTATGATGAGTTTGGCTTTGCTGGTCTGCAGGAGGGCTTTTCC
GAGGAGGCTGCAAGGCAGGCTGCGCAGGGCGGCTTCGGTGGATTTGGTGGCTTTGGCGGCAATGGCTCTTTCAGTGGCTC
ATACAGCTCAAATGGCGGACCATTTACACATCAGGAATTTCATTTTGAGAACGGCTCCGGGGATATGGATGATATTTTCT
CAATGTTCGGAGATATGTTTTCACATGGTGGCAGCTCCGGAAGTCGTGGCTCGTACAGCACAGGTGGTGCAGGGGGCTTC
GGTGGAAACAGTGCAGATTGGAGCAACTTCACAGGTGGTTCAGCGCGGGGACGTTCATCGAGCCACAAGGGTTCTGATGT
TATGGCTGACCTTACGATTTCATTTGACGAGGCTGTTTTCGGATGCGAGAAATCTATTTCGTTACAGGATCCGACCACAG
GCAAGGTTTCCAATCTGTCCATCCATATTCCGGCAGGAATCGAGTCTGGCAAGACAGTCAGACTTAAAGGACAGGGAAAT
CCGGGCAGAAACGGTGGTGCAGCCGGAGATGTGCTTCTCAACGTAAATGTGACACCGAGCAGGGAGTTTGAGCGTAAGGG
ACAGAATGTATACAGTACAATCCGTGTTCCGTTTACGACAGCGGCTCTCGGTGGTAAGGCGAGGGTGCATACTCTTTATG
GAGATGTGGAGTGCAGCATCAAGGCAGGTACACAGGGCGGCAGCAAGATACGTCTCAAGGGCAAGGGTATTTCGAGCATG
AAGAATCCTTCAGTAAAGGGAGACCAGTATGTGACTGTTGAGATTTCGGTTCCACGCAGGCTTACGCCAAGGGCTAAGGA
AGCGCTTGAGGAATACAGGGCTGTGAGCTGA

Upstream 100 bases:

>100_bases
CGGGCTGCCTGTGCAGTCCGCTTATACTATATGGTGTAATGTATATTGTTTAATTATGATGATAAATTGTTACAGGTAAA
TAGAAAGGCAGGTGAGTATA

Downstream 100 bases:

>100_bases
TGAATTTTATGTAGTTTATATATGGAAAACAAATTTGAAATCCGCTATACTATAGGTATTATAGTACAGCGGATTTTTTG
TGGCAAAATAAGAAAGGACA

Product: chaperone DnaJ domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHAEEMFKDVTEAYNVLSDEKKRKLYDEFGFAGLQEGFS
EEAARQAAQGGFGGFGGFGGNGSFSGSYSSNGGPFTHQEFHFENGSGDMDDIFSMFGDMFSHGGSSGSRGSYSTGGAGGF
GGNSADWSNFTGGSARGRSSSHKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHIPAGIESGKTVRLKGQGN
PGRNGGAAGDVLLNVNVTPSREFERKGQNVYSTIRVPFTTAALGGKARVHTLYGDVECSIKAGTQGGSKIRLKGKGISSM
KNPSVKGDQYVTVEISVPRRLTPRAKEALEEYRAVS

Sequences:

>Translated_356_residues
MAAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHAEEMFKDVTEAYNVLSDEKKRKLYDEFGFAGLQEGFS
EEAARQAAQGGFGGFGGFGGNGSFSGSYSSNGGPFTHQEFHFENGSGDMDDIFSMFGDMFSHGGSSGSRGSYSTGGAGGF
GGNSADWSNFTGGSARGRSSSHKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHIPAGIESGKTVRLKGQGN
PGRNGGAAGDVLLNVNVTPSREFERKGQNVYSTIRVPFTTAALGGKARVHTLYGDVECSIKAGTQGGSKIRLKGKGISSM
KNPSVKGDQYVTVEISVPRRLTPRAKEALEEYRAVS
>Mature_355_residues
AAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHAEEMFKDVTEAYNVLSDEKKRKLYDEFGFAGLQEGFSE
EAARQAAQGGFGGFGGFGGNGSFSGSYSSNGGPFTHQEFHFENGSGDMDDIFSMFGDMFSHGGSSGSRGSYSTGGAGGFG
GNSADWSNFTGGSARGRSSSHKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHIPAGIESGKTVRLKGQGNP
GRNGGAAGDVLLNVNVTPSREFERKGQNVYSTIRVPFTTAALGGKARVHTLYGDVECSIKAGTQGGSKIRLKGKGISSMK
NPSVKGDQYVTVEISVPRRLTPRAKEALEEYRAVS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon

COG id: COG0484

COG function: function code O; DnaJ-class molecular chaperone with C-terminal Zn finger domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360840, Length=373, Percent_Identity=30.8310991957105, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI205360838, Length=373, Percent_Identity=30.8310991957105, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI6631085, Length=371, Percent_Identity=29.3800539083558, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI5453690, Length=364, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI201862587, Length=376, Percent_Identity=28.4574468085106, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI56549115, Length=376, Percent_Identity=28.1914893617021, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI39204547, Length=358, Percent_Identity=27.9329608938547, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI201862518, Length=376, Percent_Identity=28.4574468085106, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI7706495, Length=353, Percent_Identity=26.3456090651558, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194328758, Length=303, Percent_Identity=26.4026402640264, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI194328760, Length=303, Percent_Identity=26.4026402640264, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI4504511, Length=374, Percent_Identity=25.9358288770053, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI47777312, Length=70, Percent_Identity=61.4285714285714, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI17388799, Length=66, Percent_Identity=60.6060606060606, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI9558755, Length=163, Percent_Identity=35.5828220858896, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4885495, Length=66, Percent_Identity=60.6060606060606, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI23503241, Length=74, Percent_Identity=56.7567567567568, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI21553335, Length=74, Percent_Identity=52.7027027027027, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI45504382, Length=77, Percent_Identity=48.0519480519481, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI27151736, Length=70, Percent_Identity=52.8571428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI88501736, Length=70, Percent_Identity=52.8571428571429, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI194306642, Length=151, Percent_Identity=36.4238410596026, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI194306640, Length=151, Percent_Identity=36.4238410596026, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI29126218, Length=68, Percent_Identity=50, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI5031741, Length=76, Percent_Identity=48.6842105263158, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI24308127, Length=74, Percent_Identity=45.945945945946, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI72534740, Length=157, Percent_Identity=35.6687898089172, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI56687498, Length=137, Percent_Identity=35.036496350365, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI22749377, Length=67, Percent_Identity=52.2388059701493, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68077168, Length=74, Percent_Identity=50, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI68077166, Length=74, Percent_Identity=50, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI27735107, Length=96, Percent_Identity=40.625, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787235, Length=347, Percent_Identity=29.971181556196, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786197, Length=136, Percent_Identity=38.2352941176471, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534355, Length=370, Percent_Identity=29.7297297297297, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI115532378, Length=101, Percent_Identity=41.5841584158416, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17553096, Length=66, Percent_Identity=56.0606060606061, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17553098, Length=66, Percent_Identity=56.0606060606061, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI212645553, Length=71, Percent_Identity=52.112676056338, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507263, Length=366, Percent_Identity=24.3169398907104, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554644, Length=67, Percent_Identity=55.2238805970149, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17568643, Length=78, Percent_Identity=50, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17538236, Length=67, Percent_Identity=50.7462686567164, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323870, Length=67, Percent_Identity=55.2238805970149, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324265, Length=342, Percent_Identity=25.4385964912281, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6323813, Length=168, Percent_Identity=36.9047619047619, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324321, Length=72, Percent_Identity=41.6666666666667, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322194, Length=87, Percent_Identity=41.3793103448276, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320888, Length=107, Percent_Identity=41.1214953271028, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324252, Length=71, Percent_Identity=38.0281690140845, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI85726398, Length=363, Percent_Identity=29.2011019283747, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24658562, Length=370, Percent_Identity=29.1891891891892, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24658555, Length=370, Percent_Identity=29.1891891891892, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920464, Length=351, Percent_Identity=26.2108262108262, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI45549272, Length=67, Percent_Identity=59.7014925373134, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552813, Length=67, Percent_Identity=59.7014925373134, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552811, Length=67, Percent_Identity=59.7014925373134, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654066, Length=98, Percent_Identity=47.9591836734694, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654070, Length=98, Percent_Identity=47.9591836734694, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654068, Length=98, Percent_Identity=47.9591836734694, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654072, Length=98, Percent_Identity=47.9591836734694, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18858081, Length=360, Percent_Identity=26.3888888888889, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17737735, Length=77, Percent_Identity=50.6493506493506, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24668523, Length=77, Percent_Identity=50.6493506493506, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28574723, Length=77, Percent_Identity=50.6493506493506, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129487, Length=73, Percent_Identity=50.6849315068493, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24584014, Length=73, Percent_Identity=50.6849315068493, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24668492, Length=72, Percent_Identity=45.8333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646562, Length=78, Percent_Identity=48.7179487179487, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646560, Length=78, Percent_Identity=48.7179487179487, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646558, Length=78, Percent_Identity=48.7179487179487, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24646556, Length=78, Percent_Identity=48.7179487179487, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357547, Length=78, Percent_Identity=48.7179487179487, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI161077138, Length=64, Percent_Identity=51.5625, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21358273, Length=68, Percent_Identity=51.4705882352941, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21355911, Length=121, Percent_Identity=36.3636363636364, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012724
- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095
- InterPro:   IPR001305 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C; PF00684 DnaJ_CXXCXGXG [H]

EC number: NA

Molecular weight: Translated: 37782; Mature: 37651

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHAEEMFKDVTEAYNVLSD
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
EKKRKLYDEFGFAGLQEGFSEEAARQAAQGGFGGFGGFGGNGSFSGSYSSNGGPFTHQEF
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
HFENGSGDMDDIFSMFGDMFSHGGSSGSRGSYSTGGAGGFGGNSADWSNFTGGSARGRSS
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHIPAGIESGKTVRLKGQGN
CCCCCCEEEEEEEEHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCC
PGRNGGAAGDVLLNVNVTPSREFERKGQNVYSTIRVPFTTAALGGKARVHTLYGDVECSI
CCCCCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCEEEECCCCCEEEEEEECCEEEEE
KAGTQGGSKIRLKGKGISSMKNPSVKGDQYVTVEISVPRRLTPRAKEALEEYRAVS
EECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAKRDYYETLGVNKSADKEAIKRAYRKLAKKYHPDTNAGNPHAEEMFKDVTEAYNVLSD
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
EKKRKLYDEFGFAGLQEGFSEEAARQAAQGGFGGFGGFGGNGSFSGSYSSNGGPFTHQEF
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
HFENGSGDMDDIFSMFGDMFSHGGSSGSRGSYSTGGAGGFGGNSADWSNFTGGSARGRSS
EEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SHKGSDVMADLTISFDEAVFGCEKSISLQDPTTGKVSNLSIHIPAGIESGKTVRLKGQGN
CCCCCCEEEEEEEEHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEEECCC
PGRNGGAAGDVLLNVNVTPSREFERKGQNVYSTIRVPFTTAALGGKARVHTLYGDVECSI
CCCCCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCEEEECCCCCEEEEEEECCEEEEE
KAGTQGGSKIRLKGKGISSMKNPSVKGDQYVTVEISVPRRLTPRAKEALEEYRAVS
EECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA