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The map label for this gene is eno [H]

Identifier: 238925169

GI number: 238925169

Start: 2705356

End: 2706747

Strand: Reverse

Name: eno [H]

Synonym: EUBREC_2823

Alternate gene names: 238925169

Gene position: 2706747-2705356 (Counterclockwise)

Preceding gene: 238925170

Following gene: 238925168

Centisome position: 78.46

GC content: 46.7

Gene sequence:

>1392_bases
ATGCATGCTTGCATTCAATCCTACAATCATGACATAGAGAAACTGTATTGTAGGATAAAATTGAATCAGAAAAACATAAG
GAGGTTCATAATGAATTATTTAGAAATCGAAAAGGTAGTGGGTAGAGAAATACTTGATTCCAGAGGAAATCCTACAGTTG
AGGCAGAGGTTTATCTGGTTGACGGAACTGTTGCAAGAGGCACAGCACCATCAGGAGCTTCAACAGGAGAGTTCGAGGCG
CTTGAGCTTCGTGACGGAGACAAGAGCAGATATCTGGGCAAGGGTGTTACCGGGGCAGTAGAAAATATAAATACTGTAAT
CAACGACACATTGACAGGTATGGACGCAAGTGATATCTATGCAATAGATGCGGCTATGATAGAGGCAGATGGCACAAAGG
ACAAGTCTAAGCTTGGAGCTAATGCCATTCTTGCTGTATCAATTGCGTGTGCGAGGGCGGCAGCTGTATCACTTGATATT
CCTCTCTATAGATTTTTAGGTGGTATCTCGGGAAACAGACTTCCTGTACCTATGATGAACATTGTAAACGGTGGCTGTCA
TGCACTCTCATCAGGACTTGACGTGCAGGAGTTTATGATTATGCCGGTAGGTGCGCCTTCCTTCAAGGAGTGTCTCAGAT
GGTGTGCGGAGGTATTTCATGCACTTGCAGCCATATTAAAGGAGCGCGGTCTTGCCACGTCAGTAGGTGACGAGGGTGGA
TTTGCACCGGCACTCAAATCTGACGAGGAGGCTATCGAGACAATTCTTGAAGCTGTAAAGAAGGCAGGCTATGAGCCGGG
CAAGGATTTCAAGATTGCCATGGATGCCGCATCATCAGAGTGGAAGAGTGAAAAGGGCAAGGGCTTTTACAAGCTGCCAA
AGGCCGGCACAGAGTACACCTCAGAGGAGCTTATAGAGCACTGGGCAAAGCTTTGTGACAAATATCCTATTATATCAATC
GAGGATGGTCTTGATGAGGAGGACTGGGAAGGCTGGCAGAAGCTTACTGCGCGTCTTGGCGATAGGGTACAGCTTGTGGG
AGATGACCTGTTCGTAACAAACACTGAGAGACTTGCAAAGGGAATAAGCCTTGGAGCAGGAAATGCAATCCTGATTAAAT
TAAACCAGATCGGTTCAGTATCAGAGACTCTTGAGGCTATCAAGATGGCACACAAGGCAGGCTATACAGCAATCAGCTCA
CACCGCTCAGGGGAGACTGCCGACACAACAATTGCAGACCTTGCGGTGGCACTCAACACATGCCAGATAAAGACAGGTGC
ACCTTCGCGCTCAGAGCGTGTTGCAAAGTATAACCAGCTTTTAAGAATTGAAGAGGAACTGGGAAAGAGCGCAGTTTATC
CGGGAATTAAAGCGTTTAATGTGCAGCAGTAG

Upstream 100 bases:

>100_bases
AACTAACTGTGTAGCATTTTTCTTTATTTATAAATATATGCATATGCGGCAGGCTTTCACATATGTATTTTTATGATTAA
AGGGTCAAAAGGTATCATCG

Downstream 100 bases:

>100_bases
TTTAAAATAAAACTCTATGACTGTATTCAGTCATTATCCATTGGAGTGTCTTTAGGAGAAAATAAGATAAGTAAAGTCGC
AGATATCATAATATGGTATC

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MHACIQSYNHDIEKLYCRIKLNQKNIRRFIMNYLEIEKVVGREILDSRGNPTVEAEVYLVDGTVARGTAPSGASTGEFEA
LELRDGDKSRYLGKGVTGAVENINTVINDTLTGMDASDIYAIDAAMIEADGTKDKSKLGANAILAVSIACARAAAVSLDI
PLYRFLGGISGNRLPVPMMNIVNGGCHALSSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGG
FAPALKSDEEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGFYKLPKAGTEYTSEELIEHWAKLCDKYPIISI
EDGLDEEDWEGWQKLTARLGDRVQLVGDDLFVTNTERLAKGISLGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISS
HRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ

Sequences:

>Translated_463_residues
MHACIQSYNHDIEKLYCRIKLNQKNIRRFIMNYLEIEKVVGREILDSRGNPTVEAEVYLVDGTVARGTAPSGASTGEFEA
LELRDGDKSRYLGKGVTGAVENINTVINDTLTGMDASDIYAIDAAMIEADGTKDKSKLGANAILAVSIACARAAAVSLDI
PLYRFLGGISGNRLPVPMMNIVNGGCHALSSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGG
FAPALKSDEEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGFYKLPKAGTEYTSEELIEHWAKLCDKYPIISI
EDGLDEEDWEGWQKLTARLGDRVQLVGDDLFVTNTERLAKGISLGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISS
HRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ
>Mature_463_residues
MHACIQSYNHDIEKLYCRIKLNQKNIRRFIMNYLEIEKVVGREILDSRGNPTVEAEVYLVDGTVARGTAPSGASTGEFEA
LELRDGDKSRYLGKGVTGAVENINTVINDTLTGMDASDIYAIDAAMIEADGTKDKSKLGANAILAVSIACARAAAVSLDI
PLYRFLGGISGNRLPVPMMNIVNGGCHALSSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGG
FAPALKSDEEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGFYKLPKAGTEYTSEELIEHWAKLCDKYPIISI
EDGLDEEDWEGWQKLTARLGDRVQLVGDDLFVTNTERLAKGISLGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISS
HRSGETADTTIADLAVALNTCQIKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=50.5800464037123, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.0440835266821, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=436, Percent_Identity=50.2293577981651, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI301897469, Length=436, Percent_Identity=50.2293577981651, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI301897479, Length=434, Percent_Identity=46.0829493087558, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=28.486646884273, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=28.486646884273, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=28.486646884273, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI310129182, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310110045, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310120572, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789141, Length=433, Percent_Identity=59.3533487297921, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=50.1138952164009, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=50.1138952164009, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=50, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=49.5391705069124, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=46.7741935483871, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=46.7741935483871, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=46.7741935483871, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=49.3087557603687, Blast_Score=348, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24580918, Length=449, Percent_Identity=46.5478841870824, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24580916, Length=449, Percent_Identity=46.5478841870824, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24580920, Length=449, Percent_Identity=46.5478841870824, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24580914, Length=449, Percent_Identity=46.5478841870824, Blast_Score=354, Evalue=8e-98,
Organism=Drosophila melanogaster, GI281360527, Length=447, Percent_Identity=46.5324384787472, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI17137654, Length=447, Percent_Identity=46.5324384787472, Blast_Score=352, Evalue=4e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 50106; Mature: 50106

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHACIQSYNHDIEKLYCRIKLNQKNIRRFIMNYLEIEKVVGREILDSRGNPTVEAEVYLV
CCHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
DGTVARGTAPSGASTGEFEALELRDGDKSRYLGKGVTGAVENINTVINDTLTGMDASDIY
ECCEECCCCCCCCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
AIDAAMIEADGTKDKSKLGANAILAVSIACARAAAVSLDIPLYRFLGGISGNRLPVPMMN
EEHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHEEECCHHHHHHCCCCCCCCCCCHHH
IVNGGCHALSSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGG
HHCCHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
FAPALKSDEEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGFYKLPKAGTEYT
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCCEEECCCCCCCCC
SEELIEHWAKLCDKYPIISIEDGLDEEDWEGWQKLTARLGDRVQLVGDDLFVTNTERLAK
HHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEECHHHHHH
GISLGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNT
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHH
CQIKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MHACIQSYNHDIEKLYCRIKLNQKNIRRFIMNYLEIEKVVGREILDSRGNPTVEAEVYLV
CCHHHHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
DGTVARGTAPSGASTGEFEALELRDGDKSRYLGKGVTGAVENINTVINDTLTGMDASDIY
ECCEECCCCCCCCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
AIDAAMIEADGTKDKSKLGANAILAVSIACARAAAVSLDIPLYRFLGGISGNRLPVPMMN
EEHHEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHEEECCHHHHHHCCCCCCCCCCCHHH
IVNGGCHALSSGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGG
HHCCHHHHHHCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
FAPALKSDEEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGFYKLPKAGTEYT
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCCCEEECCCCCCCCC
SEELIEHWAKLCDKYPIISIEDGLDEEDWEGWQKLTARLGDRVQLVGDDLFVTNTERLAK
HHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCEEEECHHHHHH
GISLGAGNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETADTTIADLAVALNT
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHH
CQIKTGAPSRSERVAKYNQLLRIEEELGKSAVYPGIKAFNVQQ
EEEECCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA