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The map label for this gene is ispH [H]

Identifier: 238923999

GI number: 238923999

Start: 1525028

End: 1525888

Strand: Direct

Name: ispH [H]

Synonym: EUBREC_1637

Alternate gene names: 238923999

Gene position: 1525028-1525888 (Clockwise)

Preceding gene: 238923998

Following gene: 238924000

Centisome position: 44.21

GC content: 38.21

Gene sequence:

>861_bases
ATGGGAGAGCTTACTATCGCAAAAAGCGCCGGCTTTTGTTTTGGAGTGACGAGAGCGGTAAATATGGTCTATGAGGCAAT
TGAAAAGGAAAATGTGCCTATATACACCTATGGCCCGATTATTCACAATGATGAGGTCGTAAAGGATATGGAAAAAAATG
GCGTAACCGTCATAAACGATCTCGATGAGCTTTCTTCTCATGAAAAGGGTGTGATGATTATCCGTTCACACGGTATAAGC
AAGGCTGAGTATGACAGGATAAAAAACTGTGGCTTTGAGGTGCTTAATGCAACCTGTCCTTTTGTATCAAAAATTCACAG
ATATGTGGAAGATTACAGCTCAAAGGGCTATGATATTATAATTGTCGGCAGTCCAAAGCACCCTGAGGTGTGTGGAATAA
AGGGCTGGGCAGACGAAAAATGCCATGTAACCATCATAAATTCTCCTGAGGATGCCGAAAATTACATGAAAAATAGTACA
AAAAAACTATGCATAGTGTCACAAACGACATTTAACTACAATAAATTTCAAGATATAGTTGAAATTATTGCAAAAAAAGG
TTATGATATAACTGTTTTAAATACAATCTGTAATGCTACAGAGGTCAGACAGACCGAAGCAAGAAAGGTAGCACAGTGCT
CAGATGTCATGATTGTCATAGGCGACAGGCACAGCTCCAATACACAGAAGCTATTTGAAATATGTAAAAACGAATGCAAA
AATACTTACTATATACAGACAAGTGACGAAATGGATTATACACAAATCAGGTCCAACAATAACGTAGGTATTACAGCAGG
GGCTTCAACCCCAAATAATATCATTGAGGAGGTTTCAAAGAATGTCAGAAATGAGCTTTGA

Upstream 100 bases:

>100_bases
TTTGAAGCAGGCGGATGATGCTGTGCTGCTTGATTCGTCTGATATGACAATCGATGAGGTGGTAGATAGAATGAAACAGC
TTGTGAAAGAGGCATAGCGT

Downstream 100 bases:

>100_bases
ACAAATGCTGGAAGAATCTTTTAAAACTATAAGAAATGGAGAAGTGGTAGAAGGTACCGTTATTGATGTTAAACCTGACG
AGATTTTCTTAAATATTGGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MGELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHNDEVVKDMEKNGVTVINDLDELSSHEKGVMIIRSHGIS
KAEYDRIKNCGFEVLNATCPFVSKIHRYVEDYSSKGYDIIIVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNST
KKLCIVSQTTFNYNKFQDIVEIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMIVIGDRHSSNTQKLFEICKNECK
NTYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIEEVSKNVRNEL

Sequences:

>Translated_286_residues
MGELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHNDEVVKDMEKNGVTVINDLDELSSHEKGVMIIRSHGIS
KAEYDRIKNCGFEVLNATCPFVSKIHRYVEDYSSKGYDIIIVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNST
KKLCIVSQTTFNYNKFQDIVEIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMIVIGDRHSSNTQKLFEICKNECK
NTYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIEEVSKNVRNEL
>Mature_285_residues
GELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHNDEVVKDMEKNGVTVINDLDELSSHEKGVMIIRSHGISK
AEYDRIKNCGFEVLNATCPFVSKIHRYVEDYSSKGYDIIIVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNSTK
KLCIVSQTTFNYNKFQDIVEIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMIVIGDRHSSNTQKLFEICKNECKN
TYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIEEVSKNVRNEL

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=279, Percent_Identity=29.7491039426523, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 32135; Mature: 32004

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHNDEVVKDMEKNGVTVIND
CCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHHCCCEEEEC
LDELSSHEKGVMIIRSHGISKAEYDRIKNCGFEVLNATCPFVSKIHRYVEDYSSKGYDII
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
IVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNSTKKLCIVSQTTFNYNKFQDIV
EECCCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
EIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMIVIGDRHSSNTQKLFEICKNECK
HHHHHCCCCEEEHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCC
NTYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIEEVSKNVRNEL
CEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GELTIAKSAGFCFGVTRAVNMVYEAIEKENVPIYTYGPIIHNDEVVKDMEKNGVTVIND
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHHCCCEEEEC
LDELSSHEKGVMIIRSHGISKAEYDRIKNCGFEVLNATCPFVSKIHRYVEDYSSKGYDII
HHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
IVGSPKHPEVCGIKGWADEKCHVTIINSPEDAENYMKNSTKKLCIVSQTTFNYNKFQDIV
EECCCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
EIIAKKGYDITVLNTICNATEVRQTEARKVAQCSDVMIVIGDRHSSNTQKLFEICKNECK
HHHHHCCCCEEEHHHHHCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCC
NTYYIQTSDEMDYTQIRSNNNVGITAGASTPNNIIEEVSKNVRNEL
CEEEEEECCCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]