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The map label for this gene is lepA

Identifier: 238923983

GI number: 238923983

Start: 1510610

End: 1512421

Strand: Direct

Name: lepA

Synonym: EUBREC_1621

Alternate gene names: 238923983

Gene position: 1510610-1512421 (Clockwise)

Preceding gene: 238923982

Following gene: 238923984

Centisome position: 43.79

GC content: 45.09

Gene sequence:

>1812_bases
ATGTCAATAGATCAGAGCAAAATCCGCAATTTTTGTATTATTGCCCATATTGATCACGGTAAATCTACACTCGCGGACAG
AATTATAGAAAAAACAGGCACACTTACCAGCAGGGAGATGCAGGCTCAGGTGCTCGACAATATGGATTTAGAGAGAGAGC
GTGGTATCACAATCAAGTCTCAGGCAGTCCGTATTATATATACCGCAAAGGATGGCGAAGAGTATATTTTTAATCTGATA
GATACACCGGGACACGTGGATTTTAACTATGAGGTATCAAGGAGTCTTGCGGCATGTGACGGAGCTATACTTGTAGTGGA
TGCGGCCCAGGGCGTTGAGGCCCAGACACTTGCAAATGTTTACCTTGCACTGGATCATGACCTAGATGTATTTCCTGTTA
TAAATAAGGTGGATCTGCCGAGTGCGCGCCCGGATGAGGTGGCACAGGAGATTGAGGACGTTATCGGTATTGAAGCTATG
GATGCACCGCGTATTTCCGCAAAGACAGGACTCAATATAGAGGATGTGCTGGAGCAGATTGTAAAAAAGATTCCGGCACC
AACAGGCGATAAAGATGCACCGCTTAAAGCACTTATATTTGATGCAACATATGATTCTTATAAGGGAGTCATTATTTTCT
GCCGACTGAGAGAGGGTTCTGTAAAGGTAGGAGACACAATAAAGATGATGGCTACAGGTGCATCTGATGTGGTTACAGAG
GTGGGCTATTTTGGCGCAGGCCAGTTTATACCATGTGATGAACTTTCGGCCGGTATGGTTGGATACATTGCGGCCAGCAT
CAAAAATGTCAGGGATACACGTGTGGGTGATACAGTTACACTCGTTGACAGACCGTGCGATGAGGCGCTTCCGGGCTATA
AGAAGGTCAACCCTATGGTTTACTGTGGTCTTTATCCTGCAGACAGTGCAAAGTATCCTGATTTAAGGGATGCGCTTGAA
AAGCTGCAGGTCAACGACGCTTCATTACAGTTTGAGCCTGAGACATCACTGGCACTTGGCTTTGGCTTCAGATGCGGATT
CCTTGGTCTTTTACATCTGGAAATTATTCAGGAGAGACTGGAGAGAGAGTTTGATCTTGATCTCGTTACCACTGCACCTA
GTGTTATTTACAAGATTCACAAGACAAGCGGTGAGGTGATAGATTTGACCAACCCTTCAAATATGCCGGATCCGTCAGAG
ATTGAGTATATGGAGGAGCCATACGTATCTGCAGAGATTATGGTCACAAGTGACTATGTCGGTGCGATTATGAAGCTGTG
CCAGGAGAGACGAGGAGTCTATATCAGCATGGAGTATATCGAAAAAACACGAGCTCTCATAAAGTACGACTTGCCATTAA
ATGAGATTATCTATGATTTCTTTGATGCTTTAAAGTCACGCTCGAGGGGATATGCTTCGTTTGACTATGAGATGAAGGAT
TATGAGCGCTCAGAGCTTGTCAAGCTTGACATCTTAATCAATAAGGAAATGGTCGATGCACTTTCATTTATCGTATTCAA
GGAGAGTGCATACGAAAGAGGCCGTAAGATGTGCGAGAAGCTTAAGGGTGAGATACCAAGGCATCTGTTTGAGATACCTA
TTCAGGCAGCAGTCGGTGGCAAGATTATAGCAAGAGAGACGGTTAAGGCGCTTCGCAAGGATGTGCTTGCCAAGTGCTAC
GGTGGTGATATTTCACGTAAGAAGAAGCTGCTTGAGAAGCAGAAGGAAGGAAAGAAGAGGATGCGTCAGGTGGGCAATGT
AGAGATACCACAGGAGGCATTCATGAGTGTGCTTAAGCTGGATGAGGAGTAA

Upstream 100 bases:

>100_bases
ACCACTTGCAGATATACTTGATAAAGTGTTAAAATAATATAGACTTTATTTCACAAATCTGATAAACTTTGATGAGTTAT
TGAAAGCATTAGGAGGACAT

Downstream 100 bases:

>100_bases
TCGCACATATGAAGAATCTTGAGATTTATATTCATATACCATTTTGTGTGAGAAAATGTGAATATTGTGATTTTTTATCA
TTTCCCGCAGACGATGATGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MSIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQVLDNMDLERERGITIKSQAVRIIYTAKDGEEYIFNLI
DTPGHVDFNYEVSRSLAACDGAILVVDAAQGVEAQTLANVYLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAM
DAPRISAKTGLNIEDVLEQIVKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLREGSVKVGDTIKMMATGASDVVTE
VGYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVGDTVTLVDRPCDEALPGYKKVNPMVYCGLYPADSAKYPDLRDALE
KLQVNDASLQFEPETSLALGFGFRCGFLGLLHLEIIQERLEREFDLDLVTTAPSVIYKIHKTSGEVIDLTNPSNMPDPSE
IEYMEEPYVSAEIMVTSDYVGAIMKLCQERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYASFDYEMKD
YERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLFEIPIQAAVGGKIIARETVKALRKDVLAKCY
GGDISRKKKLLEKQKEGKKRMRQVGNVEIPQEAFMSVLKLDEE

Sequences:

>Translated_603_residues
MSIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQVLDNMDLERERGITIKSQAVRIIYTAKDGEEYIFNLI
DTPGHVDFNYEVSRSLAACDGAILVVDAAQGVEAQTLANVYLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAM
DAPRISAKTGLNIEDVLEQIVKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLREGSVKVGDTIKMMATGASDVVTE
VGYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVGDTVTLVDRPCDEALPGYKKVNPMVYCGLYPADSAKYPDLRDALE
KLQVNDASLQFEPETSLALGFGFRCGFLGLLHLEIIQERLEREFDLDLVTTAPSVIYKIHKTSGEVIDLTNPSNMPDPSE
IEYMEEPYVSAEIMVTSDYVGAIMKLCQERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYASFDYEMKD
YERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLFEIPIQAAVGGKIIARETVKALRKDVLAKCY
GGDISRKKKLLEKQKEGKKRMRQVGNVEIPQEAFMSVLKLDEE
>Mature_602_residues
SIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQVLDNMDLERERGITIKSQAVRIIYTAKDGEEYIFNLID
TPGHVDFNYEVSRSLAACDGAILVVDAAQGVEAQTLANVYLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAMD
APRISAKTGLNIEDVLEQIVKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLREGSVKVGDTIKMMATGASDVVTEV
GYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVGDTVTLVDRPCDEALPGYKKVNPMVYCGLYPADSAKYPDLRDALEK
LQVNDASLQFEPETSLALGFGFRCGFLGLLHLEIIQERLEREFDLDLVTTAPSVIYKIHKTSGEVIDLTNPSNMPDPSEI
EYMEEPYVSAEIMVTSDYVGAIMKLCQERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYASFDYEMKDY
ERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLFEIPIQAAVGGKIIARETVKALRKDVLAKCYG
GDISRKKKLLEKQKEGKKRMRQVGNVEIPQEAFMSVLKLDEE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=600, Percent_Identity=50.1666666666667, Blast_Score=619, Evalue=1e-177,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=47.0588235294118, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=40.4109589041096, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=37.4233128834356, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=46.0176991150442, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=46.0176991150442, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=46.0176991150442, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI25306287, Length=136, Percent_Identity=47.7941176470588, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306283, Length=136, Percent_Identity=47.7941176470588, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI19923640, Length=136, Percent_Identity=47.7941176470588, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=37.593984962406, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=37.593984962406, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI94966752, Length=135, Percent_Identity=32.5925925925926, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729339, Length=223, Percent_Identity=25.5605381165919, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729337, Length=223, Percent_Identity=25.5605381165919, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI34147630, Length=259, Percent_Identity=28.1853281853282, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=57.4539363484087, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=541, Percent_Identity=30.6839186691312, Blast_Score=191, Evalue=2e-49,
Organism=Escherichia coli, GI1789738, Length=156, Percent_Identity=37.8205128205128, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789559, Length=227, Percent_Identity=28.1938325991189, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=241, Percent_Identity=27.8008298755187, Blast_Score=62, Evalue=8e-11,
Organism=Escherichia coli, GI1789737, Length=241, Percent_Identity=27.8008298755187, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.1764705882353, Blast_Score=476, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17556745, Length=158, Percent_Identity=37.3417721518987, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=144, Percent_Identity=39.5833333333333, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=37.1212121212121, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=37.1212121212121, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=227, Percent_Identity=29.9559471365639, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=599, Percent_Identity=42.9048414023372, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6324707, Length=145, Percent_Identity=41.3793103448276, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=145, Percent_Identity=41.3793103448276, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=39.375, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=42.1052631578947, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=37.9310344827586, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=329, Percent_Identity=26.4437689969605, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=45.2579034941764, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.1654676258993, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582462, Length=151, Percent_Identity=39.0728476821192, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=36, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=36, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=36, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=41.7218543046358, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363316, Length=236, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864358, Length=236, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921738, Length=232, Percent_Identity=28.448275862069, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_EUBR3 (C4Z9E3)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937499.1
- GeneID:   7964697
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1621
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67485; Mature: 67354

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQVLDNMDLERERGITIKS
CCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCEEEC
QAVRIIYTAKDGEEYIFNLIDTPGHVDFNYEVSRSLAACDGAILVVDAAQGVEAQTLANV
CCEEEEEEECCCHHHHHHHCCCCCCEEECHHHHHHHHHCCCEEEEEECCCCCCHHHHHEE
YLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAMDAPRISAKTGLNIEDVLEQI
EEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
VKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLREGSVKVGDTIKMMATGASDVVTE
HHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCEEECCEEEEEECCHHHHHHH
VGYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVGDTVTLVDRPCDEALPGYKKVNPMV
HHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCHHCCCEE
YCGLYPADSAKYPDLRDALEKLQVNDASLQFEPETSLALGFGFRCGFLGLLHLEIIQERL
EEEECCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHH
EREFDLDLVTTAPSVIYKIHKTSGEVIDLTNPSNMPDPSEIEYMEEPYVSAEIMVTSDYV
HHHCCCEEEECCCHHEEEEECCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEEECHHH
GAIMKLCQERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYASFDYEMKD
HHHHHHHHHHCCEEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEECCCHHC
YERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLFEIPIQAAVGG
CCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
KIIARETVKALRKDVLAKCYGGDISRKKKLLEKQKEGKKRMRQVGNVEIPQEAFMSVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
DEE
CCC
>Mature Secondary Structure 
SIDQSKIRNFCIIAHIDHGKSTLADRIIEKTGTLTSREMQAQVLDNMDLERERGITIKS
CCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHCCCEEEC
QAVRIIYTAKDGEEYIFNLIDTPGHVDFNYEVSRSLAACDGAILVVDAAQGVEAQTLANV
CCEEEEEEECCCHHHHHHHCCCCCCEEECHHHHHHHHHCCCEEEEEECCCCCCHHHHHEE
YLALDHDLDVFPVINKVDLPSARPDEVAQEIEDVIGIEAMDAPRISAKTGLNIEDVLEQI
EEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
VKKIPAPTGDKDAPLKALIFDATYDSYKGVIIFCRLREGSVKVGDTIKMMATGASDVVTE
HHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCEEECCEEEEEECCHHHHHHH
VGYFGAGQFIPCDELSAGMVGYIAASIKNVRDTRVGDTVTLVDRPCDEALPGYKKVNPMV
HHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCHHCCCEE
YCGLYPADSAKYPDLRDALEKLQVNDASLQFEPETSLALGFGFRCGFLGLLHLEIIQERL
EEEECCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHH
EREFDLDLVTTAPSVIYKIHKTSGEVIDLTNPSNMPDPSEIEYMEEPYVSAEIMVTSDYV
HHHCCCEEEECCCHHEEEEECCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEEECHHH
GAIMKLCQERRGVYISMEYIEKTRALIKYDLPLNEIIYDFFDALKSRSRGYASFDYEMKD
HHHHHHHHHHCCEEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEECCCHHC
YERSELVKLDILINKEMVDALSFIVFKESAYERGRKMCEKLKGEIPRHLFEIPIQAAVGG
CCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCC
KIIARETVKALRKDVLAKCYGGDISRKKKLLEKQKEGKKRMRQVGNVEIPQEAFMSVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
DEE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA