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The map label for this gene is prfA

Identifier: 238923860

GI number: 238923860

Start: 1368751

End: 1369827

Strand: Direct

Name: prfA

Synonym: EUBREC_1488

Alternate gene names: 238923860

Gene position: 1368751-1369827 (Clockwise)

Preceding gene: 238923859

Following gene: 238923861

Centisome position: 39.68

GC content: 46.24

Gene sequence:

>1077_bases
ATGTTTGATAAGTTAGAAGATCTTATACACCATTATGAAGAGCTGATGAATCTGCTCAGCGAGCCGGATGTGGCAAATGA
TGCAAACAGATTTAAAAAGCTTATGAAGGAGCAGAGTGACCTTGCTCCTATAGTTGAGACATATAAGAAATATAAAGAGT
GCAAGCAGAATATCGAGGATTCACTGGCTATTTTGGATGAGGAATCAGATGAAGAGATGAGAGAGCTCGCAAAGGAAGAG
CTTAAGGATTCAAAGGAGCAGGTGGAGGAGCTTGAGAAGAAGCTTAAAATACTTCTTCTGCCAAAGGACCCTAACGATGA
CAAGAACGTAATAGTCGAAATCCGTGCCGGTGCAGGCGGAGAGGAAGCAGCACTTTTTGCAGCGGAAATCTACCGTATGT
ATGTGCACTACGCAGAGAACAGAGGCTGGAAGGTGGAGACACTTGATGCCGATGAGACAGGAATCGGCGGTATGAAGTCT
GTCGAGTTCATGGTAAAGGGCTCAGGTGCATACTCTATCCTAAAGTATGAGTCGGGAGTACACCGTGTGCAGCGAGTGCC
TGAGACAGAGTCGCAGGGACGTATCCAGACCTCTACATGCTCAGTTGCTGTAATGCCTGAGGCAGAGGATGTGGATGTCA
AGATTGATGACAAGGATATCCGAATTGACGTAATGCGTGCTTCCGGAAACGGTGGACAGTGTGTCAACACAACAGATTCA
GCAGTCCGACTGACACATTATCCAACAGGTATTGTCATCTACAGCCAGACTGAGAAGTCGCAGATACAGAACAAGGAAAA
GGCTTTTGCACTTCTTCGTACAAAGCTCTATGACATGGAGCTTCAGAAGAAGCAGGATGCAGAGGCAGAGGAGAGACGAA
GCCAGATCGGTACAGGAGACCGTGCTGAGAAGATTCGTACCTACAACTTCCCACAGGGACGTGTGACAGATCACAGAATC
AATCTGACACTCTATAAGCTTGACAAGATTCTTAACGGAGATATCCAGGAAATAATTGATGCCTGTATCGCAGCCGACCA
GGCGAAGAAGTTAAGTAACATGGAGCATGATGCATAA

Upstream 100 bases:

>100_bases
ATGATGCAGTTTGCCGGATTTAGCGATGTACATGTGATAAAGGACCTGGCGGGAAATGACAGGGTAGTATCGGGAGTGTT
TGACAGCAAGGAGGAAAAAC

Downstream 100 bases:

>100_bases
ATATTTGGTATAAAAGAGTCTGAGAAAATCAGGCTCTTTTATTTTATAAACTAATATGATATAATTGTGAATAAATATTT
ATACAGAAATGAGGAATAAT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFDKLEDLIHHYEELMNLLSEPDVANDANRFKKLMKEQSDLAPIVETYKKYKECKQNIEDSLAILDEESDEEMRELAKEE
LKDSKEQVEELEKKLKILLLPKDPNDDKNVIVEIRAGAGGEEAALFAAEIYRMYVHYAENRGWKVETLDADETGIGGMKS
VEFMVKGSGAYSILKYESGVHRVQRVPETESQGRIQTSTCSVAVMPEAEDVDVKIDDKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRTKLYDMELQKKQDAEAEERRSQIGTGDRAEKIRTYNFPQGRVTDHRI
NLTLYKLDKILNGDIQEIIDACIAADQAKKLSNMEHDA

Sequences:

>Translated_358_residues
MFDKLEDLIHHYEELMNLLSEPDVANDANRFKKLMKEQSDLAPIVETYKKYKECKQNIEDSLAILDEESDEEMRELAKEE
LKDSKEQVEELEKKLKILLLPKDPNDDKNVIVEIRAGAGGEEAALFAAEIYRMYVHYAENRGWKVETLDADETGIGGMKS
VEFMVKGSGAYSILKYESGVHRVQRVPETESQGRIQTSTCSVAVMPEAEDVDVKIDDKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRTKLYDMELQKKQDAEAEERRSQIGTGDRAEKIRTYNFPQGRVTDHRI
NLTLYKLDKILNGDIQEIIDACIAADQAKKLSNMEHDA
>Mature_358_residues
MFDKLEDLIHHYEELMNLLSEPDVANDANRFKKLMKEQSDLAPIVETYKKYKECKQNIEDSLAILDEESDEEMRELAKEE
LKDSKEQVEELEKKLKILLLPKDPNDDKNVIVEIRAGAGGEEAALFAAEIYRMYVHYAENRGWKVETLDADETGIGGMKS
VEFMVKGSGAYSILKYESGVHRVQRVPETESQGRIQTSTCSVAVMPEAEDVDVKIDDKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRTKLYDMELQKKQDAEAEERRSQIGTGDRAEKIRTYNFPQGRVTDHRI
NLTLYKLDKILNGDIQEIIDACIAADQAKKLSNMEHDA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=270, Percent_Identity=47.7777777777778, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=40.3560830860534, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=42.2330097087379, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=49.7159090909091, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=37.5, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=39.2727272727273, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=325, Percent_Identity=40.6153846153846, Blast_Score=234, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19921226, Length=277, Percent_Identity=45.8483754512635, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_EUBR3 (C4Z911)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002937376.1
- GeneID:   7963853
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_1488
- ProtClustDB:   CLSK2506069
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40688; Mature: 40688

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKLEDLIHHYEELMNLLSEPDVANDANRFKKLMKEQSDLAPIVETYKKYKECKQNIED
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLAILDEESDEEMRELAKEELKDSKEQVEELEKKLKILLLPKDPNDDKNVIVEIRAGAGG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAEIYRMYVHYAENRGWKVETLDADETGIGGMKSVEFMVKGSGAYSILKYESGV
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEHHHH
HRVQRVPETESQGRIQTSTCSVAVMPEAEDVDVKIDDKDIRIDVMRASGNGGQCVNTTDS
HHHHCCCCCCCCCEEEECEEEEEECCCCCCCEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRTKLYDMELQKKQDAEAEERRSQIGTGD
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
RAEKIRTYNFPQGRVTDHRINLTLYKLDKILNGDIQEIIDACIAADQAKKLSNMEHDA
HHHHHEECCCCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFDKLEDLIHHYEELMNLLSEPDVANDANRFKKLMKEQSDLAPIVETYKKYKECKQNIED
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLAILDEESDEEMRELAKEELKDSKEQVEELEKKLKILLLPKDPNDDKNVIVEIRAGAGG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCC
EEAALFAAEIYRMYVHYAENRGWKVETLDADETGIGGMKSVEFMVKGSGAYSILKYESGV
CHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEEHHHH
HRVQRVPETESQGRIQTSTCSVAVMPEAEDVDVKIDDKDIRIDVMRASGNGGQCVNTTDS
HHHHCCCCCCCCCEEEECEEEEEECCCCCCCEEEECCCEEEEEEEEECCCCCCEECCCCC
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRTKLYDMELQKKQDAEAEERRSQIGTGD
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
RAEKIRTYNFPQGRVTDHRINLTLYKLDKILNGDIQEIIDACIAADQAKKLSNMEHDA
HHHHHEECCCCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA