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The map label for this gene is rho [H]

Identifier: 238923856

GI number: 238923856

Start: 1365130

End: 1366470

Strand: Direct

Name: rho [H]

Synonym: EUBREC_1484

Alternate gene names: 238923856

Gene position: 1365130-1366470 (Clockwise)

Preceding gene: 238923852

Following gene: 238923857

Centisome position: 39.57

GC content: 44.74

Gene sequence:

>1341_bases
ATGGAACAGATGAGAGAAAAATATGAAAGTTTGTCGGCATTTGTGCTTAAGGATCTGGCGAAGGCAAGAGGAATTAAGGG
CGTTTCATCACTGAAAAAGGGACAGATTATTGAAAGAATGCTCGAGGAGGATGCAAAAGAGGCTGAGGAGGCTGAGAAAA
ACGGTACAGCCGAGGAGCGCGCCAACTCTTTTAAAGATGATTATGCAGCACTCGACAGTGGAGAAGAGGCAGAAGGCATA
CTTGAGGTAATGCCGGAGGGCTTTGGATTTATCCGCTGTGATAATTATATGCCGGGAGATCATGATATCTATGTGGCACC
TGCGCAGATACGCCGCTTCAATTTAAAGACAGGTGATATCGTAAAGGGTAATATGAAGGTGAAGTCTGAGCGTGAGAAAT
TCCAGGCTCTTTTATACCTGACAACTGTCAACGGATATACACCGGATGTAGCACAGAAGCGTACAAGCTTTGAGGATCTT
GTGCCTATATTCCCGAATGAGAGACTGCGTCTCGAGAGACCTGGAGCAAGTGTTGCGATGAGGGTTGTAGATCTCATCTC
TCCAATAGGAAAGGGACAGCGAGGCATGATCGTTTCACAGCCTAAGGCCGGAAAGACCACATTACTTAAAGAGGTTGCAA
AGTCTGTCACTGTAAACAATCCTGAGATGCATCTTATTATTCTCCTTATTGATGAGAGACCGGAGGAGGTTACAGATATC
AAGGAGGCTATAGAGGGGAACAATGTCGAGGTAATCTACTCTACATTTGATGAACTTCCTGAGCATCACAAGAGAGTGTC
AGAGATGGTAATAGAGAGAGCAAAGCGCCTTGTAGAGCATGGTAATGATGTCATGATACTCCTTGACAGCATAACAAGGC
TTGCAAGAGCTTACAATGTGACAGTTCCACCATCAGGCCGTACACTTTCAGGAGGACTTGATCCTGTAGCGCTTCATATG
CCAAAGAGATTTTTCGGAGCAGCACGTAATATGAGAAACGGCGGCAGTCTGACAATACTTGCAACAGCTCTTGTTGATAC
CGGAAGCAAGATGGATGATGTAGTATTTGAGGAGTTCAAGGGAACAGGAAACATGGAGCTTGTGCTCGACAGAAAGCTTT
CCGAGAAGAGGATCTTCCCTGCTATAGATATAGTAAAATCAGGAACAAGGCGTGAGGATCTGCTCCTTGATGAGGATGAG
CAGGCAGCAGTCGATAATATGCGTAAAGCCTTCAACGGCATGCGCTCTGATGAGGCTGTTGAGAACGTACTCAACATGTT
TGCACACACAAAGAGCAACAAGGAATTTGTTAATCTTGTAAAGAAAAACAGAATTTTATAA

Upstream 100 bases:

>100_bases
TCAGGCGTTATTTCCCAATAGCTACACAGATATATTTGTTAAATAATATTGTTTAGACGAATTGAATAAATACTGTTATC
AAATTTAAGGAGAAGACGAA

Downstream 100 bases:

>100_bases
AAATGTCTTGCAATTGACAGATTCATGTGCTACAATAATTAAGCTGTTTATCGGGATGTAAACGGACGCAACGTGTCGCT
CTTGCAGCAAGTATCATATG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEEDAKEAEEAEKNGTAEERANSFKDDYAALDSGEEAEGI
LEVMPEGFGFIRCDNYMPGDHDIYVAPAQIRRFNLKTGDIVKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDL
VPIFPNERLRLERPGASVAMRVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTVNNPEMHLIILLIDERPEEVTDI
KEAIEGNNVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGNDVMILLDSITRLARAYNVTVPPSGRTLSGGLDPVALHM
PKRFFGAARNMRNGGSLTILATALVDTGSKMDDVVFEEFKGTGNMELVLDRKLSEKRIFPAIDIVKSGTRREDLLLDEDE
QAAVDNMRKAFNGMRSDEAVENVLNMFAHTKSNKEFVNLVKKNRIL

Sequences:

>Translated_446_residues
MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEEDAKEAEEAEKNGTAEERANSFKDDYAALDSGEEAEGI
LEVMPEGFGFIRCDNYMPGDHDIYVAPAQIRRFNLKTGDIVKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDL
VPIFPNERLRLERPGASVAMRVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTVNNPEMHLIILLIDERPEEVTDI
KEAIEGNNVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGNDVMILLDSITRLARAYNVTVPPSGRTLSGGLDPVALHM
PKRFFGAARNMRNGGSLTILATALVDTGSKMDDVVFEEFKGTGNMELVLDRKLSEKRIFPAIDIVKSGTRREDLLLDEDE
QAAVDNMRKAFNGMRSDEAVENVLNMFAHTKSNKEFVNLVKKNRIL
>Mature_446_residues
MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEEDAKEAEEAEKNGTAEERANSFKDDYAALDSGEEAEGI
LEVMPEGFGFIRCDNYMPGDHDIYVAPAQIRRFNLKTGDIVKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDL
VPIFPNERLRLERPGASVAMRVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTVNNPEMHLIILLIDERPEEVTDI
KEAIEGNNVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGNDVMILLDSITRLARAYNVTVPPSGRTLSGGLDPVALHM
PKRFFGAARNMRNGGSLTILATALVDTGSKMDDVVFEEFKGTGNMELVLDRKLSEKRIFPAIDIVKSGTRREDLLLDEDE
QAAVDNMRKAFNGMRSDEAVENVLNMFAHTKSNKEFVNLVKKNRIL

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=433, Percent_Identity=49.6535796766744, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 49974; Mature: 49974

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEEDAKEAEEAEKNGTAEER
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ANSFKDDYAALDSGEEAEGILEVMPEGFGFIRCDNYMPGDHDIYVAPAQIRRFNLKTGDI
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEHHHHEEEECCCCCE
VKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDLVPIFPNERLRLERPGASVAM
EECCCEEHHHHHHHHEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCCEEEECCCHHHHH
RVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTVNNPEMHLIILLIDERPEEVTDI
HHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHH
KEAIEGNNVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGNDVMILLDSITRLARAYNV
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEE
TVPPSGRTLSGGLDPVALHMPKRFFGAARNMRNGGSLTILATALVDTGSKMDDVVFEEFK
ECCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHC
GTGNMELVLDRKLSEKRIFPAIDIVKSGTRREDLLLDEDEQAAVDNMRKAFNGMRSDEAV
CCCCEEEEEECCCCHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
ENVLNMFAHTKSNKEFVNLVKKNRIL
HHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MEQMREKYESLSAFVLKDLAKARGIKGVSSLKKGQIIERMLEEDAKEAEEAEKNGTAEER
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ANSFKDDYAALDSGEEAEGILEVMPEGFGFIRCDNYMPGDHDIYVAPAQIRRFNLKTGDI
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEHHHHEEEECCCCCE
VKGNMKVKSEREKFQALLYLTTVNGYTPDVAQKRTSFEDLVPIFPNERLRLERPGASVAM
EECCCEEHHHHHHHHEEEEEEECCCCCCHHHHHHCCHHHCCCCCCCCCEEEECCCHHHHH
RVVDLISPIGKGQRGMIVSQPKAGKTTLLKEVAKSVTVNNPEMHLIILLIDERPEEVTDI
HHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHH
KEAIEGNNVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGNDVMILLDSITRLARAYNV
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEE
TVPPSGRTLSGGLDPVALHMPKRFFGAARNMRNGGSLTILATALVDTGSKMDDVVFEEFK
ECCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHC
GTGNMELVLDRKLSEKRIFPAIDIVKSGTRREDLLLDEDEQAAVDNMRKAFNGMRSDEAV
CCCCEEEEEECCCCHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
ENVLNMFAHTKSNKEFVNLVKKNRIL
HHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]