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The map label for this gene is pcrA [H]

Identifier: 238923773

GI number: 238923773

Start: 1272616

End: 1274907

Strand: Direct

Name: pcrA [H]

Synonym: EUBREC_1395

Alternate gene names: 238923773

Gene position: 1272616-1274907 (Clockwise)

Preceding gene: 238923771

Following gene: 238923774

Centisome position: 36.89

GC content: 46.25

Gene sequence:

>2292_bases
ATGAACTTAGATATGTTGAATGAACAGCAGAGGGAGGCCGTTTTGACCACAGAGGGACCGCTTCTTATTTTAGCGGGGGC
AGGCAGTGGCAAGACCAGGGTTCTTACATACAGGACTGCCTACCTGATAGATGAGTGTGGCGTCAATCCTTACAATATCA
TGGCAATCACCTTTACAAACAAGGCTGCGGGAGAGATGCGTGAGCGAATTGATGATATGGTGGGCTATGGTTCGGAAAGC
ATATGGGTTTCTACATTCCATTCGACATGTGTGCGTATACTGCGAAGGTATATTGACAGGCTGGGATATGACACAAATTT
TACCATATATGATGCTGACGACCAGAAGGCTCTGATGAAGGACATCTGCAAGCGTCTTGAAATAGATACCAAGATGTATA
AGGAAAAGATGTTTTTAAATGTCATTTCATCTGCAAAGGATGAGATGATTGATCCGATAGAGTTTGAAAACCGCTTCACA
GGTGACTTCGTGAAGCGCAAGCAGGCACTTGTATACAAGGAGTATCAGAATGCCCTAAAGCAGAATAACGCACTCGATTT
TGATGATTTGCTTGTGAAGACAGTAGAGCTTTTTAAGCTGGATAAAGAGGTGCTGGATTACTATCAGGAGCGCTTCAGAT
ATATCATGGTGGATGAGTATCAGGACACAAACACCGTGCAGTTTGAGCTTATCAGGCTTCTTGCCATGAAGTATAAGAAT
CTGTGTGTGGTTGGTGATGATGATCAGTCCATTTACAAGTTCAGAGGTGCCAATATCTACAATATCTTAAACTTTGAGAA
GCATTTTGAAGATGCAAAGGTCATAAAGCTTGAGCAGAATTACAGGTCTACCCAGAATATCCTAGATGCCGCCAACAGCG
TCATTTCCAACAACGTGGGTAGAAAGGACAAGCGCCTTTGGACTGACAACGGAGCCGGAGACAGGATTACCTTTGAACAG
CTTGAGAGTGGCTTCGAGGAAGCGGATTATGTGGCGCGTTCGATAGCGAGACTCGTGAGAAAGGGTGAGGCGCGCTATAA
GGACTGTGCTGTGCTTTACCGAACCAACGCACAGTCGCGTCTCTTCGAGGAGAAATTTATTGCAGCCAACATACCATACA
AGATTGTCGGCGGCGTGAATTTCTATGCCAGAAAAGAGATAAAGGATATCCTTGCATATTTAAAGACTATTGACAATGGC
CATGATGACCTTGCGGTAAAGCGAATAATAAATGTGCCAAAGCGCGGCATCGGAGCTACAAGCATCAATAAGGTGACAGA
CTATGCCATTGAGAAGGGAATAGATTTCTATACGGCACTGCGCTATGTCGATGAGGTGCCGGGCATGGCGAGGTCAGCAG
GCAAGATAAAGCCTTTTGTGATGTTTATACAGTCACTTCGTGCGAGAGCCGAGATGGATTCGGTTTCGCAGCTCATACAG
GCAATAATCGATGAGACCGGCTATGTCGATGAGCTGAAGGCAGAGGGCACGGATGAAGCAGAGCAGCGTATTGAGAATAT
TGATGAGCTTATAAACAAGGCTGTTGATTACGAGCAGGGGGAGGAGAATCCTACTCTCAGTGGCTTTTTGGAGGAGGTAG
CGCTTGTAGCCGATATAGACGGTCTTGACGAAAACAGCGACTATGTGGTTCTTATGACTCTGCACAGTGCTAAGGGACTT
GAGTTTCCGAATGTGTATCTGGCGGGCATGGAGGATGGTCTTTTCCCAAGCTATATGTCAATCACGAGTGATGATGCGAC
AAGCGAGATAGAGGAGGAAAGGCGTCTTGCTTATGTTGGCATCACGAGAGCCAAGGAGCATCTGACCATCACATCTGCAC
GCATGCGTATGGTGCGTGGAGAGACACAGTTCAACAAGGTATCGCGATTTGTGAAGGAGATTCCGCGTGAGCTTATGTCA
GGTGAGGTCTATGAGCCAAAGCGCCGTGATGATGACATTGAAAGAAATTCACAGAGCACCTACAGAAAGGCTAAGGAGGC
CTTCAAAAAGACACCTACATACGGCACAGAGTATGCGACCACCTTTAATACACAGCGTACAAGGACACCTGTCTACACGC
CTGTGAGCAATCAGAAGTCATTTGCCAGTGCCAATACCACCGGCGGCCTTTCATATAAGGTAGGAGACAGAGTATCACAC
ATCAAGTTTGGCGCGGGAGAGGTCATGGCAATTGTAGAAGGCGGCAGGGATTATGAGGTCACTGTGGACTTTGACAAGGC
CGGTACCAAAAAGATGTTTGCTTCATTTGCCAAATTGAAAAAAATTGACTAA

Upstream 100 bases:

>100_bases
GTTGTGCAAAACATATGTTTGTGGTATGCTGTAGATAAATTATTTTTGTATTTATGGTTTGTGTGCCTGTAATAAGACTG
GAGAATTGAGAGGAAAACAG

Downstream 100 bases:

>100_bases
AATTATTGTAATTTTTATAGGAAAATTGGATATAATATGCTATAATGGATTTAACAAATAGCATTTGACAATTACTTTTG
GAAAAGGAGCGGATTGAATT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MNLDMLNEQQREAVLTTEGPLLILAGAGSGKTRVLTYRTAYLIDECGVNPYNIMAITFTNKAAGEMRERIDDMVGYGSES
IWVSTFHSTCVRILRRYIDRLGYDTNFTIYDADDQKALMKDICKRLEIDTKMYKEKMFLNVISSAKDEMIDPIEFENRFT
GDFVKRKQALVYKEYQNALKQNNALDFDDLLVKTVELFKLDKEVLDYYQERFRYIMVDEYQDTNTVQFELIRLLAMKYKN
LCVVGDDDQSIYKFRGANIYNILNFEKHFEDAKVIKLEQNYRSTQNILDAANSVISNNVGRKDKRLWTDNGAGDRITFEQ
LESGFEEADYVARSIARLVRKGEARYKDCAVLYRTNAQSRLFEEKFIAANIPYKIVGGVNFYARKEIKDILAYLKTIDNG
HDDLAVKRIINVPKRGIGATSINKVTDYAIEKGIDFYTALRYVDEVPGMARSAGKIKPFVMFIQSLRARAEMDSVSQLIQ
AIIDETGYVDELKAEGTDEAEQRIENIDELINKAVDYEQGEENPTLSGFLEEVALVADIDGLDENSDYVVLMTLHSAKGL
EFPNVYLAGMEDGLFPSYMSITSDDATSEIEEERRLAYVGITRAKEHLTITSARMRMVRGETQFNKVSRFVKEIPRELMS
GEVYEPKRRDDDIERNSQSTYRKAKEAFKKTPTYGTEYATTFNTQRTRTPVYTPVSNQKSFASANTTGGLSYKVGDRVSH
IKFGAGEVMAIVEGGRDYEVTVDFDKAGTKKMFASFAKLKKID

Sequences:

>Translated_763_residues
MNLDMLNEQQREAVLTTEGPLLILAGAGSGKTRVLTYRTAYLIDECGVNPYNIMAITFTNKAAGEMRERIDDMVGYGSES
IWVSTFHSTCVRILRRYIDRLGYDTNFTIYDADDQKALMKDICKRLEIDTKMYKEKMFLNVISSAKDEMIDPIEFENRFT
GDFVKRKQALVYKEYQNALKQNNALDFDDLLVKTVELFKLDKEVLDYYQERFRYIMVDEYQDTNTVQFELIRLLAMKYKN
LCVVGDDDQSIYKFRGANIYNILNFEKHFEDAKVIKLEQNYRSTQNILDAANSVISNNVGRKDKRLWTDNGAGDRITFEQ
LESGFEEADYVARSIARLVRKGEARYKDCAVLYRTNAQSRLFEEKFIAANIPYKIVGGVNFYARKEIKDILAYLKTIDNG
HDDLAVKRIINVPKRGIGATSINKVTDYAIEKGIDFYTALRYVDEVPGMARSAGKIKPFVMFIQSLRARAEMDSVSQLIQ
AIIDETGYVDELKAEGTDEAEQRIENIDELINKAVDYEQGEENPTLSGFLEEVALVADIDGLDENSDYVVLMTLHSAKGL
EFPNVYLAGMEDGLFPSYMSITSDDATSEIEEERRLAYVGITRAKEHLTITSARMRMVRGETQFNKVSRFVKEIPRELMS
GEVYEPKRRDDDIERNSQSTYRKAKEAFKKTPTYGTEYATTFNTQRTRTPVYTPVSNQKSFASANTTGGLSYKVGDRVSH
IKFGAGEVMAIVEGGRDYEVTVDFDKAGTKKMFASFAKLKKID
>Mature_763_residues
MNLDMLNEQQREAVLTTEGPLLILAGAGSGKTRVLTYRTAYLIDECGVNPYNIMAITFTNKAAGEMRERIDDMVGYGSES
IWVSTFHSTCVRILRRYIDRLGYDTNFTIYDADDQKALMKDICKRLEIDTKMYKEKMFLNVISSAKDEMIDPIEFENRFT
GDFVKRKQALVYKEYQNALKQNNALDFDDLLVKTVELFKLDKEVLDYYQERFRYIMVDEYQDTNTVQFELIRLLAMKYKN
LCVVGDDDQSIYKFRGANIYNILNFEKHFEDAKVIKLEQNYRSTQNILDAANSVISNNVGRKDKRLWTDNGAGDRITFEQ
LESGFEEADYVARSIARLVRKGEARYKDCAVLYRTNAQSRLFEEKFIAANIPYKIVGGVNFYARKEIKDILAYLKTIDNG
HDDLAVKRIINVPKRGIGATSINKVTDYAIEKGIDFYTALRYVDEVPGMARSAGKIKPFVMFIQSLRARAEMDSVSQLIQ
AIIDETGYVDELKAEGTDEAEQRIENIDELINKAVDYEQGEENPTLSGFLEEVALVADIDGLDENSDYVVLMTLHSAKGL
EFPNVYLAGMEDGLFPSYMSITSDDATSEIEEERRLAYVGITRAKEHLTITSARMRMVRGETQFNKVSRFVKEIPRELMS
GEVYEPKRRDDDIERNSQSTYRKAKEAFKKTPTYGTEYATTFNTQRTRTPVYTPVSNQKSFASANTTGGLSYKVGDRVSH
IKFGAGEVMAIVEGGRDYEVTVDFDKAGTKKMFASFAKLKKID

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=764, Percent_Identity=38.4816753926702, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI48994965, Length=654, Percent_Identity=39.1437308868502, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=28.3625730994152, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=27.8082191780822, Blast_Score=214, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6324477, Length=694, Percent_Identity=23.1988472622478, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 87153; Mature: 87153

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDMLNEQQREAVLTTEGPLLILAGAGSGKTRVLTYRTAYLIDECGVNPYNIMAITFTN
CCCCCCCHHHHCEEEECCCCEEEEEECCCCCEEEEEEEHHHHHHHCCCCCEEEEEEEECC
KAAGEMRERIDDMVGYGSESIWVSTFHSTCVRILRRYIDRLGYDTNFTIYDADDQKALMK
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
DICKRLEIDTKMYKEKMFLNVISSAKDEMIDPIEFENRFTGDFVKRKQALVYKEYQNALK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QNNALDFDDLLVKTVELFKLDKEVLDYYQERFRYIMVDEYQDTNTVQFELIRLLAMKYKN
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCC
LCVVGDDDQSIYKFRGANIYNILNFEKHFEDAKVIKLEQNYRSTQNILDAANSVISNNVG
EEEECCCCCHHHHCCCCCEEEECCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
RKDKRLWTDNGAGDRITFEQLESGFEEADYVARSIARLVRKGEARYKDCAVLYRTNAQSR
CCCCCEECCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCHHH
LFEEKFIAANIPYKIVGGVNFYARKEIKDILAYLKTIDNGHDDLAVKRIINVPKRGIGAT
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
SINKVTDYAIEKGIDFYTALRYVDEVPGMARSAGKIKPFVMFIQSLRARAEMDSVSQLIQ
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AIIDETGYVDELKAEGTDEAEQRIENIDELINKAVDYEQGEENPTLSGFLEEVALVADID
HHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
GLDENSDYVVLMTLHSAKGLEFPNVYLAGMEDGLFPSYMSITSDDATSEIEEERRLAYVG
CCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHCEEEEE
ITRAKEHLTITSARMRMVRGETQFNKVSRFVKEIPRELMSGEVYEPKRRDDDIERNSQST
EHHHHHHEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHH
YRKAKEAFKKTPTYGTEYATTFNTQRTRTPVYTPVSNQKSFASANTTGGLSYKVGDRVSH
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHH
IKFGAGEVMAIVEGGRDYEVTVDFDKAGTKKMFASFAKLKKID
EECCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLDMLNEQQREAVLTTEGPLLILAGAGSGKTRVLTYRTAYLIDECGVNPYNIMAITFTN
CCCCCCCHHHHCEEEECCCCEEEEEECCCCCEEEEEEEHHHHHHHCCCCCEEEEEEEECC
KAAGEMRERIDDMVGYGSESIWVSTFHSTCVRILRRYIDRLGYDTNFTIYDADDQKALMK
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
DICKRLEIDTKMYKEKMFLNVISSAKDEMIDPIEFENRFTGDFVKRKQALVYKEYQNALK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QNNALDFDDLLVKTVELFKLDKEVLDYYQERFRYIMVDEYQDTNTVQFELIRLLAMKYKN
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCC
LCVVGDDDQSIYKFRGANIYNILNFEKHFEDAKVIKLEQNYRSTQNILDAANSVISNNVG
EEEECCCCCHHHHCCCCCEEEECCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCC
RKDKRLWTDNGAGDRITFEQLESGFEEADYVARSIARLVRKGEARYKDCAVLYRTNAQSR
CCCCCEECCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCHHH
LFEEKFIAANIPYKIVGGVNFYARKEIKDILAYLKTIDNGHDDLAVKRIINVPKRGIGAT
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
SINKVTDYAIEKGIDFYTALRYVDEVPGMARSAGKIKPFVMFIQSLRARAEMDSVSQLIQ
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AIIDETGYVDELKAEGTDEAEQRIENIDELINKAVDYEQGEENPTLSGFLEEVALVADID
HHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
GLDENSDYVVLMTLHSAKGLEFPNVYLAGMEDGLFPSYMSITSDDATSEIEEERRLAYVG
CCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCHHHHCCCCCHHHHHHHHHCEEEEE
ITRAKEHLTITSARMRMVRGETQFNKVSRFVKEIPRELMSGEVYEPKRRDDDIERNSQST
EHHHHHHEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHH
YRKAKEAFKKTPTYGTEYATTFNTQRTRTPVYTPVSNQKSFASANTTGGLSYKVGDRVSH
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHH
IKFGAGEVMAIVEGGRDYEVTVDFDKAGTKKMFASFAKLKKID
EECCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA