Click here to switch to the map view.

The map label for this gene is aspS [H]

Identifier: 238923547

GI number: 238923547

Start: 1074072

End: 1075457

Strand: Direct

Name: aspS [H]

Synonym: EUBREC_1167

Alternate gene names: 238923547

Gene position: 1074072-1075457 (Clockwise)

Preceding gene: 238923546

Following gene: 238923548

Centisome position: 31.14

GC content: 46.54

Gene sequence:

>1386_bases
ATGCTCCGGGGCTTTTTTTCCGTCCCGCTTAAAAAATCAAAGGAGCACAAAAATATGGAATTCTTAACAGGAGCAGTTCA
AAAGGAAGACCTCGGTATCGCAGAAATTCTTGCAGGCGACTACGAGGGCAAAAACATCAAAGTAAAGGGTGCGATTCACA
CCATAAGAGATATGGGTGAGGTTGCATTCATCGTCTTAAGACGCAGGGACGGACTCGTACAGTGTGTATACGAGCCGGGC
AAATCCCAGTTTAGCGTCGATGATCTAAAGGAAGCAGATACCGTGGAGGTATGCGGCACATACAAGAGTGATGACCGTTC
TCCAAACGGCTTCGAGCTTCGTCTTGACACTATCATAATTTTATCTGAGCCGGCGGAGCCTATGCCGCTTCAGATCAACA
AATGGAAACTGAATACTTCGCTTGAGGCAAAGCTCAATAACAGGGCAGTGGCACTCAGAAATCCAAGAGAGCGTGCTACG
TTCCGTATACAGGAGGGCATCACAAGGGGCTTTAGGGATTTCCTTTATAATAATGGATTTACAGAGATTCATACGCCAAA
GCTTGGTGCAAAGTCAGCCGAGGGAGGCGCCAACCTCTTCAAGCTTGAATATTTCCACAGACCGGCTATATTACAGCAGA
GCCCGCAGTTTTACAAGCAGATGATGGTAGGTGTCTTTGACAGGGTCTTTGAGACTGCACCTGTTTTCAGAGCAGAAAAG
CACAATACAAAGAGACATCTGAATGAGTATACATCACTCGATTTCGAGATGGGCTACATTGACGGCTTCGAGGATATCAT
GGAGATGGAGACCGGTTTTTTGCAGTACACCATGGAGCTTTTAAAGACCTCATACGCCAAGGAGCTTGAGCTTTTAAAGA
TTAAGCTGCCTGACGTGACAAGCATACCAAGAGTCAGATTTGACGAGGCAAAGCAGCGTGTTGCAGAAAAATATAACAGA
CAGTTCAGAAACCCATTCGACTTAGAGCCGGAGGAGGAGGTACTTATCGGACAGTACTTCAAGGAAGAGTATGGCAGTGA
TTTTGTATTTGTCACACATTATCCTTCAAAGAAGAGACCGTTCTATGCCATGGATGATCCGGCAGACGAGACATACACCT
TAAGCTTTGACCTGCTTTTCAGAGGTCTTGAGATCACAACAGGCGGCCAGCGAATCCACGACTACAACAAGCTTATGGAA
AAAATCGCAAAGCGTGGCATGGAGACAGAGGGTATGGAGAGCTATCTTTCAGCCTTTAAGTACGGCATGCCGCCACATGG
AGGTCTTGGAATAGGACTTGAGAGACTTACTATGCAGCTTATCGGAGAGGACAATGTGCGTGAGGCAACACTGTTTCCTC
GTGATCTCAGCAGATTGGAGCCATAG

Upstream 100 bases:

>100_bases
GGATAAGAGTGGAGAAACAGGAACAGTCCTTTTTCTCAAAGCGGGTGGTACCGCGGAATATTTTTAGAATTTTCGCCCCG
GAGCATTTCATTAATTTGAT

Downstream 100 bases:

>100_bases
GTCACAGCTTCTGGGGTTGTGGCGGGTGCGCATGCAGGCTGTGTGGTATTACAGGCTGTGAGCGTCGGAACTGTTAAAAA
TTTGTCTTCGCAATATATAT

Product: aspartyl-tRNA synthetase, non-discriminating

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLRGFFSVPLKKSKEHKNMEFLTGAVQKEDLGIAEILAGDYEGKNIKVKGAIHTIRDMGEVAFIVLRRRDGLVQCVYEPG
KSQFSVDDLKEADTVEVCGTYKSDDRSPNGFELRLDTIIILSEPAEPMPLQINKWKLNTSLEAKLNNRAVALRNPRERAT
FRIQEGITRGFRDFLYNNGFTEIHTPKLGAKSAEGGANLFKLEYFHRPAILQQSPQFYKQMMVGVFDRVFETAPVFRAEK
HNTKRHLNEYTSLDFEMGYIDGFEDIMEMETGFLQYTMELLKTSYAKELELLKIKLPDVTSIPRVRFDEAKQRVAEKYNR
QFRNPFDLEPEEEVLIGQYFKEEYGSDFVFVTHYPSKKRPFYAMDDPADETYTLSFDLLFRGLEITTGGQRIHDYNKLME
KIAKRGMETEGMESYLSAFKYGMPPHGGLGIGLERLTMQLIGEDNVREATLFPRDLSRLEP

Sequences:

>Translated_461_residues
MLRGFFSVPLKKSKEHKNMEFLTGAVQKEDLGIAEILAGDYEGKNIKVKGAIHTIRDMGEVAFIVLRRRDGLVQCVYEPG
KSQFSVDDLKEADTVEVCGTYKSDDRSPNGFELRLDTIIILSEPAEPMPLQINKWKLNTSLEAKLNNRAVALRNPRERAT
FRIQEGITRGFRDFLYNNGFTEIHTPKLGAKSAEGGANLFKLEYFHRPAILQQSPQFYKQMMVGVFDRVFETAPVFRAEK
HNTKRHLNEYTSLDFEMGYIDGFEDIMEMETGFLQYTMELLKTSYAKELELLKIKLPDVTSIPRVRFDEAKQRVAEKYNR
QFRNPFDLEPEEEVLIGQYFKEEYGSDFVFVTHYPSKKRPFYAMDDPADETYTLSFDLLFRGLEITTGGQRIHDYNKLME
KIAKRGMETEGMESYLSAFKYGMPPHGGLGIGLERLTMQLIGEDNVREATLFPRDLSRLEP
>Mature_461_residues
MLRGFFSVPLKKSKEHKNMEFLTGAVQKEDLGIAEILAGDYEGKNIKVKGAIHTIRDMGEVAFIVLRRRDGLVQCVYEPG
KSQFSVDDLKEADTVEVCGTYKSDDRSPNGFELRLDTIIILSEPAEPMPLQINKWKLNTSLEAKLNNRAVALRNPRERAT
FRIQEGITRGFRDFLYNNGFTEIHTPKLGAKSAEGGANLFKLEYFHRPAILQQSPQFYKQMMVGVFDRVFETAPVFRAEK
HNTKRHLNEYTSLDFEMGYIDGFEDIMEMETGFLQYTMELLKTSYAKELELLKIKLPDVTSIPRVRFDEAKQRVAEKYNR
QFRNPFDLEPEEEVLIGQYFKEEYGSDFVFVTHYPSKKRPFYAMDDPADETYTLSFDLLFRGLEITTGGQRIHDYNKLME
KIAKRGMETEGMESYLSAFKYGMPPHGGLGIGLERLTMQLIGEDNVREATLFPRDLSRLEP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=444, Percent_Identity=34.6846846846847, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI4758762, Length=435, Percent_Identity=28.735632183908, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI226491502, Length=446, Percent_Identity=28.0269058295964, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI40789249, Length=289, Percent_Identity=30.1038062283737, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1789256, Length=456, Percent_Identity=27.4122807017544, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787161, Length=467, Percent_Identity=24.8394004282655, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790571, Length=455, Percent_Identity=27.6923076923077, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788173, Length=304, Percent_Identity=26.9736842105263, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17551876, Length=489, Percent_Identity=35.1738241308793, Blast_Score=267, Evalue=9e-72,
Organism=Caenorhabditis elegans, GI71984122, Length=454, Percent_Identity=27.5330396475771, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17556372, Length=318, Percent_Identity=29.874213836478, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994340, Length=343, Percent_Identity=25.3644314868805, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=343, Percent_Identity=25.3644314868805, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=343, Percent_Identity=25.3644314868805, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=266, Percent_Identity=25.5639097744361, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323011, Length=454, Percent_Identity=34.8017621145374, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6321807, Length=441, Percent_Identity=29.4784580498866, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319872, Length=467, Percent_Identity=26.9807280513919, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17136276, Length=453, Percent_Identity=34.6578366445916, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI19921528, Length=440, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922416, Length=470, Percent_Identity=22.5531914893617, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640849, Length=471, Percent_Identity=25.0530785562633, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640851, Length=471, Percent_Identity=25.0530785562633, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24584738, Length=209, Percent_Identity=29.1866028708134, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 53149; Mature: 53149

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGFFSVPLKKSKEHKNMEFLTGAVQKEDLGIAEILAGDYEGKNIKVKGAIHTIRDMGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHH
VAFIVLRRRDGLVQCVYEPGKSQFSVDDLKEADTVEVCGTYKSDDRSPNGFELRLDTIII
EEEEEEECCCCEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEEEE
LSEPAEPMPLQINKWKLNTSLEAKLNNRAVALRNPRERATFRIQEGITRGFRDFLYNNGF
EECCCCCCCEEEEEEEECCEEEEECCCCEEEECCCHHHHHEEHHHHHHHHHHHHHHCCCC
TEIHTPKLGAKSAEGGANLFKLEYFHRPAILQQSPQFYKQMMVGVFDRVFETAPVFRAEK
CEECCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
HNTKRHLNEYTSLDFEMGYIDGFEDIMEMETGFLQYTMELLKTSYAKELELLKIKLPDVT
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
SIPRVRFDEAKQRVAEKYNRQFRNPFDLEPEEEVLIGQYFKEEYGSDFVFVTHYPSKKRP
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
FYAMDDPADETYTLSFDLLFRGLEITTGGQRIHDYNKLMEKIAKRGMETEGMESYLSAFK
EEECCCCCCCEEEEEHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YGMPPHGGLGIGLERLTMQLIGEDNVREATLFPRDLSRLEP
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHCCC
>Mature Secondary Structure
MLRGFFSVPLKKSKEHKNMEFLTGAVQKEDLGIAEILAGDYEGKNIKVKGAIHTIRDMGE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHH
VAFIVLRRRDGLVQCVYEPGKSQFSVDDLKEADTVEVCGTYKSDDRSPNGFELRLDTIII
EEEEEEECCCCEEEEEECCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEEEE
LSEPAEPMPLQINKWKLNTSLEAKLNNRAVALRNPRERATFRIQEGITRGFRDFLYNNGF
EECCCCCCCEEEEEEEECCEEEEECCCCEEEECCCHHHHHEEHHHHHHHHHHHHHHCCCC
TEIHTPKLGAKSAEGGANLFKLEYFHRPAILQQSPQFYKQMMVGVFDRVFETAPVFRAEK
CEECCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
HNTKRHLNEYTSLDFEMGYIDGFEDIMEMETGFLQYTMELLKTSYAKELELLKIKLPDVT
CCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
SIPRVRFDEAKQRVAEKYNRQFRNPFDLEPEEEVLIGQYFKEEYGSDFVFVTHYPSKKRP
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
FYAMDDPADETYTLSFDLLFRGLEITTGGQRIHDYNKLMEKIAKRGMETEGMESYLSAFK
EEECCCCCCCEEEEEHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YGMPPHGGLGIGLERLTMQLIGEDNVREATLFPRDLSRLEP
CCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]