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The map label for this gene is lacF [H]

Identifier: 238923455

GI number: 238923455

Start: 980430

End: 981347

Strand: Direct

Name: lacF [H]

Synonym: EUBREC_1075

Alternate gene names: 238923455

Gene position: 980430-981347 (Clockwise)

Preceding gene: 238923454

Following gene: 238923456

Centisome position: 28.42

GC content: 40.52

Gene sequence:

>918_bases
ATGAGAAAGAGTGGAGGTAAAGCGGTCAGATATAACAAATGGGGCTATATCTTTCTGATTCCGTTTATCGTTGTATACGT
AGTATTTCAGCTGGTACCGTTAATCACTACCATTTACAACAGTTTTTTTGAAAATTATATGTCGGGTCTTACACAGGTAG
GCCCAAACTTTGTTGGATTTGAAAACTATGAAAAATTGTTCACATCCGGTGATATCTGGCAGTATTTTAAGAACACAATG
GTTCTTTGGATCATGTGCTTCATACCGCAGATATTCTTATCACTGCTCCTTGGAGCATGGTTTTCGGATGTCAGCCTAAA
GCTCAAGGGAACAAGATTTTTTAAAACAGTTATATATCTGCCAAATCTGATTATGGCTTCAGCATTTTCACTGTTATTCT
TTACATTGTTTGCTGATGGGGGACCGATAAACAATCTGCTTATACAGATAGGCTTCATCTCAGAGCCGTACAAGTTCCTG
TCGCATGTTGGCAGTACAAGAGGACTCATCGCTTTGATGAACTGTCTGATGTGGTTTGGTAATACAACCATCCTGCTTAT
GGCAGGTATGCTTGGAATCGATACATCACTTTTTGAGGCAGCAGAGGTGGACGGAGCAACATCCACACAGGTGTTCTTTA
AGATAACACTTCCGCTCCTTAGACCGATACTGATATATGTAATCATTACATCACTTATCGGTGGCTTACAGCTCTTTGAT
GTACCACAGATTTTGACAAACGGAACCGGTAATCCGGTCAGATCGAGCATGACACTTATCATGTACTTAAACAAACGTCT
GTACAGTAAGGATTATGGAATGTCCGGTGCACTGTCAGTTATACTGTTTATCATCACAGCAATACTTAGTGTGGCGGTAT
TCAAGCTAAGCGGCAACGATGATAAGAGAAAGGAGTAG

Upstream 100 bases:

>100_bases
TGCGTAAGCAGGCAGGCGGGGTAATATTTTTAAAATCAATAAAATCAGTTAACAGCATTACATAACCATTGACTACAATA
CCAGTTGGGAGGTAGGTATT

Downstream 100 bases:

>100_bases
GTCGATATGGCAGCAAATGTAAACAAAAAAAAGAAAGGTATGGGAATCCATGCCAGACGTGCATTAGCATATATCGTGCT
TGTGATAATCTCTTTCTTCT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRKSGGKAVRYNKWGYIFLIPFIVVYVVFQLVPLITTIYNSFFENYMSGLTQVGPNFVGFENYEKLFTSGDIWQYFKNTM
VLWIMCFIPQIFLSLLLGAWFSDVSLKLKGTRFFKTVIYLPNLIMASAFSLLFFTLFADGGPINNLLIQIGFISEPYKFL
SHVGSTRGLIALMNCLMWFGNTTILLMAGMLGIDTSLFEAAEVDGATSTQVFFKITLPLLRPILIYVIITSLIGGLQLFD
VPQILTNGTGNPVRSSMTLIMYLNKRLYSKDYGMSGALSVILFIITAILSVAVFKLSGNDDKRKE

Sequences:

>Translated_305_residues
MRKSGGKAVRYNKWGYIFLIPFIVVYVVFQLVPLITTIYNSFFENYMSGLTQVGPNFVGFENYEKLFTSGDIWQYFKNTM
VLWIMCFIPQIFLSLLLGAWFSDVSLKLKGTRFFKTVIYLPNLIMASAFSLLFFTLFADGGPINNLLIQIGFISEPYKFL
SHVGSTRGLIALMNCLMWFGNTTILLMAGMLGIDTSLFEAAEVDGATSTQVFFKITLPLLRPILIYVIITSLIGGLQLFD
VPQILTNGTGNPVRSSMTLIMYLNKRLYSKDYGMSGALSVILFIITAILSVAVFKLSGNDDKRKE
>Mature_305_residues
MRKSGGKAVRYNKWGYIFLIPFIVVYVVFQLVPLITTIYNSFFENYMSGLTQVGPNFVGFENYEKLFTSGDIWQYFKNTM
VLWIMCFIPQIFLSLLLGAWFSDVSLKLKGTRFFKTVIYLPNLIMASAFSLLFFTLFADGGPINNLLIQIGFISEPYKFL
SHVGSTRGLIALMNCLMWFGNTTILLMAGMLGIDTSLFEAAEVDGATSTQVFFKITLPLLRPILIYVIITSLIGGLQLFD
VPQILTNGTGNPVRSSMTLIMYLNKRLYSKDYGMSGALSVILFIITAILSVAVFKLSGNDDKRKE

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=301, Percent_Identity=25.9136212624585, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790465, Length=275, Percent_Identity=25.8181818181818, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787570, Length=259, Percent_Identity=26.6409266409266, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34297; Mature: 34297

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSGGKAVRYNKWGYIFLIPFIVVYVVFQLVPLITTIYNSFFENYMSGLTQVGPNFVGF
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ENYEKLFTSGDIWQYFKNTMVLWIMCFIPQIFLSLLLGAWFSDVSLKLKGTRFFKTVIYL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHH
PNLIMASAFSLLFFTLFADGGPINNLLIQIGFISEPYKFLSHVGSTRGLIALMNCLMWFG
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHC
NTTILLMAGMLGIDTSLFEAAEVDGATSTQVFFKITLPLLRPILIYVIITSLIGGLQLFD
CHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VPQILTNGTGNPVRSSMTLIMYLNKRLYSKDYGMSGALSVILFIITAILSVAVFKLSGND
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKRKE
CCCCC
>Mature Secondary Structure
MRKSGGKAVRYNKWGYIFLIPFIVVYVVFQLVPLITTIYNSFFENYMSGLTQVGPNFVGF
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
ENYEKLFTSGDIWQYFKNTMVLWIMCFIPQIFLSLLLGAWFSDVSLKLKGTRFFKTVIYL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHH
PNLIMASAFSLLFFTLFADGGPINNLLIQIGFISEPYKFLSHVGSTRGLIALMNCLMWFG
HHHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHC
NTTILLMAGMLGIDTSLFEAAEVDGATSTQVFFKITLPLLRPILIYVIITSLIGGLQLFD
CHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VPQILTNGTGNPVRSSMTLIMYLNKRLYSKDYGMSGALSVILFIITAILSVAVFKLSGND
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DKRKE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]