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The map label for this gene is afsQ2 [H]

Identifier: 238923342

GI number: 238923342

Start: 857557

End: 858951

Strand: Direct

Name: afsQ2 [H]

Synonym: EUBREC_0959

Alternate gene names: 238923342

Gene position: 857557-858951 (Clockwise)

Preceding gene: 238923341

Following gene: 238923343

Centisome position: 24.86

GC content: 43.15

Gene sequence:

>1395_bases
ATGAAGCTTCGTAGCAGGATAACGATTGGAACACTTGGATTTTTGCTGATTTCTCTTACACTGTGCTGCACACTTATGAT
ATATGTCAGCAAAAAAAATATGTTAAACAGTACCACAAGCTATACAAAGACAGAATTGGAAAAGCTTGTGGCTAATTTTT
ATACAAACAAAAGTGACATAGAAAGCACAGTTGAAGAGCTTACAGCGGAGACAAAGCTGAAATACTACTTTTTTAAGCAG
ACGCAGTATTCGGATTCTGACACGGAGTATGTGCTGCAGTGTGGTGATGAGATAATATATAACGATTCCGGACTTGATGT
GCCAACGATACTTGGGCTGAATGAGGATAAGAGTGATATTGAGATGCAGGGGGAGACGAAAAGCGACATCGTGCATACAG
AAGGGGCAGACTATTACATCTGCGGAGTGGATATCAATATCAATGACAGAATCTATCAGGTATGTATAGTGCGCGATATA
ACAGAGCTTTACAGACAGATATACCAGATGATTGCGCTTTGTGTGGGGATTGGAATTGTTATCTCATTAGCTGCGGCAGT
AAGTATGATTGTTTTCCTGAAAAAATTACTAAGACCGTTAGAGCAGCTTAAGGATGAGGCTGATGCCATATCACAGGGGC
AGTATCAGCGTCAGATAGACGTAAAAGGAAAGGATGAGCTGGCATCACTCTCGCACAGCTTTAATACGATGGCAAAGGCT
GTAGAGTCACATATCGCAGAGGTTGAGGAGACCTCTGAGGCGAGAAACAGGCTCATACATGCGCTCTCCCATGAGATGAG
GACTCCGGTAACGGCGATTTGCGGCTATGCCTATTCGCTTCGTAATATGAAGCTGACTGATGCACAAAAGCAAGAAGCGC
TCGAATTTATGGACATTGAGGCAAAAAGACTCGGCAGCCTGTCGGGCAAGCTGACTGCTCTGGTCGGACTTACAGCGGAT
AAGATAGAGCTTAAGGACATAGAGCTTGAGGAGCTGAAAAAACATCTTGAGATGATATGGCAAGGCCGGGACGATATATT
TCTGACAGTTGAAAATGGCAGCATAAGGGGCGACAAAGATATGCTTGTCATGCTCATAACCAATCTTTGTGACAATGCAA
AAAAGGCAGGTGCGACAAGGATTGAAGTAAATATAACAGGCAGTGGAATATCGGTAAAGGACAACGGAAGAGGAATTTCA
AAGGAGGATATGAAGCATATATTTGAGATATTTTATCAGGGAGATGCTTCGAGAAATCAGGAGGGCTTTGGACTGGGACT
GGCATTGTGCCAGAGGATTGCGGAACTTCACCACTCAAAGCTTTCTGTGGAAAGCAGCTTGCAGGAGGGCAGTATTTTTT
CTTTACAATTCTCTTACAACTCTTTGATGACTTGA

Upstream 100 bases:

>100_bases
AGACGAGAACTGTCGATGTGCATGTGGCGGCATTACGCAAGAAGACAGGACTTCGTATAGTAGCTGTGCCAAAGATTGGA
TATCGTCTGGAGGTAGGAGA

Downstream 100 bases:

>100_bases
AAACATAAAAGGGGATAAAATCATATCATCAAAGAAAACGAGGAGGAACTTATGAAACGAAAAATAGTTTGTCTGATGGC
TGCAATAATGTGTTTTTCAA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKLRSRITIGTLGFLLISLTLCCTLMIYVSKKNMLNSTTSYTKTELEKLVANFYTNKSDIESTVEELTAETKLKYYFFKQ
TQYSDSDTEYVLQCGDEIIYNDSGLDVPTILGLNEDKSDIEMQGETKSDIVHTEGADYYICGVDININDRIYQVCIVRDI
TELYRQIYQMIALCVGIGIVISLAAAVSMIVFLKKLLRPLEQLKDEADAISQGQYQRQIDVKGKDELASLSHSFNTMAKA
VESHIAEVEETSEARNRLIHALSHEMRTPVTAICGYAYSLRNMKLTDAQKQEALEFMDIEAKRLGSLSGKLTALVGLTAD
KIELKDIELEELKKHLEMIWQGRDDIFLTVENGSIRGDKDMLVMLITNLCDNAKKAGATRIEVNITGSGISVKDNGRGIS
KEDMKHIFEIFYQGDASRNQEGFGLGLALCQRIAELHHSKLSVESSLQEGSIFSLQFSYNSLMT

Sequences:

>Translated_464_residues
MKLRSRITIGTLGFLLISLTLCCTLMIYVSKKNMLNSTTSYTKTELEKLVANFYTNKSDIESTVEELTAETKLKYYFFKQ
TQYSDSDTEYVLQCGDEIIYNDSGLDVPTILGLNEDKSDIEMQGETKSDIVHTEGADYYICGVDININDRIYQVCIVRDI
TELYRQIYQMIALCVGIGIVISLAAAVSMIVFLKKLLRPLEQLKDEADAISQGQYQRQIDVKGKDELASLSHSFNTMAKA
VESHIAEVEETSEARNRLIHALSHEMRTPVTAICGYAYSLRNMKLTDAQKQEALEFMDIEAKRLGSLSGKLTALVGLTAD
KIELKDIELEELKKHLEMIWQGRDDIFLTVENGSIRGDKDMLVMLITNLCDNAKKAGATRIEVNITGSGISVKDNGRGIS
KEDMKHIFEIFYQGDASRNQEGFGLGLALCQRIAELHHSKLSVESSLQEGSIFSLQFSYNSLMT
>Mature_464_residues
MKLRSRITIGTLGFLLISLTLCCTLMIYVSKKNMLNSTTSYTKTELEKLVANFYTNKSDIESTVEELTAETKLKYYFFKQ
TQYSDSDTEYVLQCGDEIIYNDSGLDVPTILGLNEDKSDIEMQGETKSDIVHTEGADYYICGVDININDRIYQVCIVRDI
TELYRQIYQMIALCVGIGIVISLAAAVSMIVFLKKLLRPLEQLKDEADAISQGQYQRQIDVKGKDELASLSHSFNTMAKA
VESHIAEVEETSEARNRLIHALSHEMRTPVTAICGYAYSLRNMKLTDAQKQEALEFMDIEAKRLGSLSGKLTALVGLTAD
KIELKDIELEELKKHLEMIWQGRDDIFLTVENGSIRGDKDMLVMLITNLCDNAKKAGATRIEVNITGSGISVKDNGRGIS
KEDMKHIFEIFYQGDASRNQEGFGLGLALCQRIAELHHSKLSVESSLQEGSIFSLQFSYNSLMT

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism. May activate AfsQ1 by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=28.695652173913, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=28.2051282051282, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=28.2157676348548, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=29.0909090909091, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787894, Length=323, Percent_Identity=25.3869969040248, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=24.4979919678715, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=25.5506607929515, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789149, Length=355, Percent_Identity=21.9718309859155, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788279, Length=279, Percent_Identity=24.3727598566308, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=26.271186440678, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789403, Length=305, Percent_Identity=27.5409836065574, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52067; Mature: 52067

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRSRITIGTLGFLLISLTLCCTLMIYVSKKNMLNSTTSYTKTELEKLVANFYTNKSDI
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
ESTVEELTAETKLKYYFFKQTQYSDSDTEYVLQCGDEIIYNDSGLDVPTILGLNEDKSDI
HHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCEEECCCCCCCCC
EMQGETKSDIVHTEGADYYICGVDININDRIYQVCIVRDITELYRQIYQMIALCVGIGIV
EECCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLAAAVSMIVFLKKLLRPLEQLKDEADAISQGQYQRQIDVKGKDELASLSHSFNTMAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
VESHIAEVEETSEARNRLIHALSHEMRTPVTAICGYAYSLRNMKLTDAQKQEALEFMDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKRLGSLSGKLTALVGLTADKIELKDIELEELKKHLEMIWQGRDDIFLTVENGSIRGDKD
HHHHCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHH
MLVMLITNLCDNAKKAGATRIEVNITGSGISVKDNGRGISKEDMKHIFEIFYQGDASRNQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
EGFGLGLALCQRIAELHHSKLSVESSLQEGSIFSLQFSYNSLMT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCC
>Mature Secondary Structure
MKLRSRITIGTLGFLLISLTLCCTLMIYVSKKNMLNSTTSYTKTELEKLVANFYTNKSDI
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
ESTVEELTAETKLKYYFFKQTQYSDSDTEYVLQCGDEIIYNDSGLDVPTILGLNEDKSDI
HHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCCEEECCCCCCCCC
EMQGETKSDIVHTEGADYYICGVDININDRIYQVCIVRDITELYRQIYQMIALCVGIGIV
EECCCCCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISLAAAVSMIVFLKKLLRPLEQLKDEADAISQGQYQRQIDVKGKDELASLSHSFNTMAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHH
VESHIAEVEETSEARNRLIHALSHEMRTPVTAICGYAYSLRNMKLTDAQKQEALEFMDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AKRLGSLSGKLTALVGLTADKIELKDIELEELKKHLEMIWQGRDDIFLTVENGSIRGDKD
HHHHCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHH
MLVMLITNLCDNAKKAGATRIEVNITGSGISVKDNGRGISKEDMKHIFEIFYQGDASRNQ
HHHHHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCC
EGFGLGLALCQRIAELHHSKLSVESSLQEGSIFSLQFSYNSLMT
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]