| Definition | Eubacterium rectale ATCC 33656, complete genome. |
|---|---|
| Accession | NC_012781 |
| Length | 3,449,685 |
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The map label for this gene is hsdM [H]
Identifier: 238923269
GI number: 238923269
Start: 783290
End: 784891
Strand: Direct
Name: hsdM [H]
Synonym: EUBREC_0880
Alternate gene names: 238923269
Gene position: 783290-784891 (Clockwise)
Preceding gene: 238923268
Following gene: 238923270
Centisome position: 22.71
GC content: 37.89
Gene sequence:
>1602_bases ATGGCGGAACAGGAAAATAGTAAGGATTTAATCAGTGTACTTTGGAGTGGCGCTGATGTACTTCGTTCTAAAATGGACGC GAATGAATATAAGAATTATCTTTTGGGAATTGTATTCTATAAATACCTATCAGATTCATTTTTGATAAGAGTATATGATT TGATAAATGATGAGAAACCGGAATCGCTCAAGGTGGCACTCGAGGCATATAAAAATGAGTTGAAGGGTGAATATGCTAAT GATTTGCTTGATGAATTAAAGCAGGATCGTAAATATGTAATTGAGCCTGAGCTTACATATACATGTTTTGCAGAGGATGC AAGGAATAATTGCTTTAATCGTGAGCAATTACAGAAAGCGTTCAATAATATTGAGCAGAGCGGAGAGTTGTTTGTGGATT TGTTTTCTGACATTGATTTGTATTCAAGCAGGCTTGGAGCGGGAGACCAGAAACAAAGTGATACAATTGCAGAACTGATA AAGGTAATAGACCAGGCGGATTTGCTCAATTCCGATGGTGAAATACTTGGAGATGCATATGAATATCTGATAGGACAGTT TGCGTCAGAGACCGGTAAGAAGGCGGGAGAATTCTATACACCACAGGCAGTATCAAAAATTCTTACTAGAATAGCAATAA CAGGACAGGAGAATGTGAAAGGTTTGTCAATCTATGATCCATGTATGGGCTCAGGTTCGCTGCTTCTTAACGCAAAGAGA TACTATAAAGGTGATACAAACTATATAAAGTATTATGGACAGGAACTGAACATGTCTACATATAATCTGGCGAGGATGAA TATGTTCCTGCATGATGTAGCTGCGGAGAATCAGAATCTGCATCATGGAGATACTCTTGATGCAGACTGGCCAACTGGTG AGGAAACTGACTTTCACATGGTACTTATGAATCCACCGTATTCTGCAAAATGGAGTGCTGCATCAGGATTTTTACAGGAC GAAAGATTTTCAGAATATGGTGTTTTGGCGCCAAAATCAAAGGCTGATTATGCATTTTTACTTCATGGACTTTATCATCT GAAAAGCAATGGAACAATGGCCATCGTTTTGCCACATGGAGTGCTTTTCAGAGGTGCAGCAGAGGGAAAAATAAGAGAAA AGCTCCTTCGTTCAGGAAACATATATGCGGTTATAGGATTGCCGGCTAATTTATTTTATAATACGTCTATTCCTACATGC ATTGTTGTCCTTAAAAAGCATAGAGATGGCAGAGATGTACTGTTTATTGATGCGTCAAAGAAGTTCATTAAGGGCAAAAA ACAAAACGAAATGTCAGACGAACATATTGATGAAGTTATGGATTTGTATAACAGAAGAGAAACTGTAGACAAAGAATCCT ATCTGGCAAGCTTTGATGATATTGAAAAGAATGATTTCAACCTAAACATACCGCGATATGTAGACAATTTTGAAAAGGAA GAGGAAGTCGATATAAATGGTCTGCTCGAGGATATGCAAAAAACAGATGACGAGATAGGAAAGGTCGAAGGTGAGTTTGT GAATTTGTTAAAAGACCTGACATCGTCCGATGAAAATATAATGGCATCGTTGAATCGTTATATTGATATGATTGAGGGGT GA
Upstream 100 bases:
>100_bases ATCAGTGTGCGGATGCAGGTAAGGTTAGGAAGCTTGCGGAAGAGGTAAGTGGAGTGTAATATTAAAGCAAAAGATTATAG AATTTCGGAGGAAGCAATTA
Downstream 100 bases:
>100_bases TTTGTATGGAAAATCCAAAGATTAGATTTAAAGGGTTTACTAAGGATTGGGAACAGCGTAAGTTGAACGAAGTGGCGGAA AAAATATGCGTAGGATTTGT
Product: type I restriction-modification system methylation subunit
Products: NA
Alternate protein names: M.EcoR124II [H]
Number of amino acids: Translated: 533; Mature: 532
Protein sequence:
>533_residues MAEQENSKDLISVLWSGADVLRSKMDANEYKNYLLGIVFYKYLSDSFLIRVYDLINDEKPESLKVALEAYKNELKGEYAN DLLDELKQDRKYVIEPELTYTCFAEDARNNCFNREQLQKAFNNIEQSGELFVDLFSDIDLYSSRLGAGDQKQSDTIAELI KVIDQADLLNSDGEILGDAYEYLIGQFASETGKKAGEFYTPQAVSKILTRIAITGQENVKGLSIYDPCMGSGSLLLNAKR YYKGDTNYIKYYGQELNMSTYNLARMNMFLHDVAAENQNLHHGDTLDADWPTGEETDFHMVLMNPPYSAKWSAASGFLQD ERFSEYGVLAPKSKADYAFLLHGLYHLKSNGTMAIVLPHGVLFRGAAEGKIREKLLRSGNIYAVIGLPANLFYNTSIPTC IVVLKKHRDGRDVLFIDASKKFIKGKKQNEMSDEHIDEVMDLYNRRETVDKESYLASFDDIEKNDFNLNIPRYVDNFEKE EEVDINGLLEDMQKTDDEIGKVEGEFVNLLKDLTSSDENIMASLNRYIDMIEG
Sequences:
>Translated_533_residues MAEQENSKDLISVLWSGADVLRSKMDANEYKNYLLGIVFYKYLSDSFLIRVYDLINDEKPESLKVALEAYKNELKGEYAN DLLDELKQDRKYVIEPELTYTCFAEDARNNCFNREQLQKAFNNIEQSGELFVDLFSDIDLYSSRLGAGDQKQSDTIAELI KVIDQADLLNSDGEILGDAYEYLIGQFASETGKKAGEFYTPQAVSKILTRIAITGQENVKGLSIYDPCMGSGSLLLNAKR YYKGDTNYIKYYGQELNMSTYNLARMNMFLHDVAAENQNLHHGDTLDADWPTGEETDFHMVLMNPPYSAKWSAASGFLQD ERFSEYGVLAPKSKADYAFLLHGLYHLKSNGTMAIVLPHGVLFRGAAEGKIREKLLRSGNIYAVIGLPANLFYNTSIPTC IVVLKKHRDGRDVLFIDASKKFIKGKKQNEMSDEHIDEVMDLYNRRETVDKESYLASFDDIEKNDFNLNIPRYVDNFEKE EEVDINGLLEDMQKTDDEIGKVEGEFVNLLKDLTSSDENIMASLNRYIDMIEG >Mature_532_residues AEQENSKDLISVLWSGADVLRSKMDANEYKNYLLGIVFYKYLSDSFLIRVYDLINDEKPESLKVALEAYKNELKGEYAND LLDELKQDRKYVIEPELTYTCFAEDARNNCFNREQLQKAFNNIEQSGELFVDLFSDIDLYSSRLGAGDQKQSDTIAELIK VIDQADLLNSDGEILGDAYEYLIGQFASETGKKAGEFYTPQAVSKILTRIAITGQENVKGLSIYDPCMGSGSLLLNAKRY YKGDTNYIKYYGQELNMSTYNLARMNMFLHDVAAENQNLHHGDTLDADWPTGEETDFHMVLMNPPYSAKWSAASGFLQDE RFSEYGVLAPKSKADYAFLLHGLYHLKSNGTMAIVLPHGVLFRGAAEGKIREKLLRSGNIYAVIGLPANLFYNTSIPTCI VVLKKHRDGRDVLFIDASKKFIKGKKQNEMSDEHIDEVMDLYNRRETVDKESYLASFDDIEKNDFNLNIPRYVDNFEKEE EVDINGLLEDMQKTDDEIGKVEGEFVNLLKDLTSSDENIMASLNRYIDMIEG
Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]
COG id: COG0286
COG function: function code V; Type I restriction-modification system methyltransferase subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
Organism=Escherichia coli, GI1790808, Length=322, Percent_Identity=27.3291925465839, Blast_Score=103, Evalue=2e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022749 - InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 - InterPro: IPR004546 [H]
Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 60642; Mature: 60511
Theoretical pI: Translated: 4.45; Mature: 4.45
Prosite motif: PS00092 N6_MTASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAEQENSKDLISVLWSGADVLRSKMDANEYKNYLLGIVFYKYLSDSFLIRVYDLINDEKP CCCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC ESLKVALEAYKNELKGEYANDLLDELKQDRKYVIEPELTYTCFAEDARNNCFNREQLQKA HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCEEEEECCHHHCCCCCHHHHHHH FNNIEQSGELFVDLFSDIDLYSSRLGAGDQKQSDTIAELIKVIDQADLLNSDGEILGDAY HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH EYLIGQFASETGKKAGEFYTPQAVSKILTRIAITGQENVKGLSIYDPCMGSGSLLLNAKR HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEHH YYKGDTNYIKYYGQELNMSTYNLARMNMFLHDVAAENQNLHHGDTLDADWPTGEETDFHM HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE VLMNPPYSAKWSAASGFLQDERFSEYGVLAPKSKADYAFLLHGLYHLKSNGTMAIVLPHG EEECCCCCCCCCHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCC VLFRGAAEGKIREKLLRSGNIYAVIGLPANLFYNTSIPTCIVVLKKHRDGRDVLFIDASK EEEECCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEHHHHCCCCCCEEEEEECCH KFIKGKKQNEMSDEHIDEVMDLYNRRETVDKESYLASFDDIEKNDFNLNIPRYVDNFEKE HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCC EEVDINGLLEDMQKTDDEIGKVEGEFVNLLKDLTSSDENIMASLNRYIDMIEG CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC >Mature Secondary Structure AEQENSKDLISVLWSGADVLRSKMDANEYKNYLLGIVFYKYLSDSFLIRVYDLINDEKP CCCCCHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC ESLKVALEAYKNELKGEYANDLLDELKQDRKYVIEPELTYTCFAEDARNNCFNREQLQKA HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCEEEEECCHHHCCCCCHHHHHHH FNNIEQSGELFVDLFSDIDLYSSRLGAGDQKQSDTIAELIKVIDQADLLNSDGEILGDAY HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH EYLIGQFASETGKKAGEFYTPQAVSKILTRIAITGQENVKGLSIYDPCMGSGSLLLNAKR HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEHH YYKGDTNYIKYYGQELNMSTYNLARMNMFLHDVAAENQNLHHGDTLDADWPTGEETDFHM HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEE VLMNPPYSAKWSAASGFLQDERFSEYGVLAPKSKADYAFLLHGLYHLKSNGTMAIVLPHG EEECCCCCCCCCHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCC VLFRGAAEGKIREKLLRSGNIYAVIGLPANLFYNTSIPTCIVVLKKHRDGRDVLFIDASK EEEECCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCEEHHHHCCCCCCEEEEEECCH KFIKGKKQNEMSDEHIDEVMDLYNRRETVDKESYLASFDDIEKNDFNLNIPRYVDNFEKE HHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCC EEVDINGLLEDMQKTDDEIGKVEGEFVNLLKDLTSSDENIMASLNRYIDMIEG CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2784505 [H]