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The map label for this gene is ydeM [C]

Identifier: 238923154

GI number: 238923154

Start: 687471

End: 688847

Strand: Direct

Name: ydeM [C]

Synonym: EUBREC_0747

Alternate gene names: 238923154

Gene position: 687471-688847 (Clockwise)

Preceding gene: 238923153

Following gene: 238923155

Centisome position: 19.93

GC content: 27.45

Gene sequence:

>1377_bases
ATGGATGAAATGTTAGTTGAAAAGTATACAGATGAAGAAAATCATATAGTGTATAAAAAATGCAAAGCCTTTACTAATGG
TAAAAAATATTTTTTATTAAACGAATTAGGGTATTCAATATTGATTGATCATGAATTGTTTAATATGTTGCAGCAAAAAA
AAGTATCTACAGATTTTGCGAGTAAGTTATTACAGCATCGACTGGCTGAATTTGATAATTTAAATCATTGTGATTTATGT
ACAAATTATAAGATTCGTCCAGAATTTTTTATGATTGATTTGACAACTCGCTGTAATTTGAATTGTAAATATTGTTTTCG
AGATGTAAACAATCCTGAGAATTATAAATCTATTTCTAAAGAAATGCTTAATGAAATTTGTAAATATATTTTTGATTATT
GTACGAAACATGAGGTTAAGAAAATATCTATTCAACCATGGGGTGGAGAACCTTTACTTGAAATGGAAAAAATTAAATGG
ATGTATGATTTTTTTGAAAATACACCTATATCTGTGCATTTTACGATTGAAACCAACGGAGTACTACTCACAGAAGAAAA
TGTAAAATGGTTATTTGAACATAATGTGTCAATAGGAATAAGTATTGATGGTAATAAAGAAATTCATGATCGGCAAAGAG
TGTTTGCAAATACGAAAGGGTCGTTTGAATATGTACTTCAAGGAATAAAAAATGTAAAGAAATATTATGGAGATAATTTT
GGAATTATTAACACAATAACTCGGAACAGTATTGAGCATATAGAGGATATATTGGATTTTTATGCAAAAGATATTAATAT
AAAACATGTTAAATTTAATTTTGTACACGAGAGTGAATTTGCAGCTAACCTATGTTTGAATAAAGAACAGGTAATAGATG
GAACAAAAAGAGTCTATAAGAAAATTATGTCTTTAAATAATGAAGGATATAATATTATAGATATGAATATTCGGCATAAA
TTATTGAACTTATTAATACGTGATAATTCAGATATTTGCGTATCACAAGGCTGTACAGGTGGAACAAGTATGATTGTGTT
TGACATGGAGGGCTACATTTATCCTTGTGAACTAACGGATTATATAGAAGAAAGAATTGGAAATGTTAAAGAAGCAGTAG
ATTTGATTAAGATAGTAAAAGAGGCAAAAGAGACAAAAGCATATTTTAAAGAAAAAGACGATGTTAGTTGTTTGTCATGC
TCATGGAGATATTTTTGCAAAGGTGGTTGTAGCATAAAAGCAAAATTATCAAGAACGAAAAAAGATGATTTGGAATGTAC
GATAAATAGTACTTTATATCCAGAAATGATTGAATCAATTTTAAGTGAACAAAAATTGATAAATGACTTTCTAAATATGA
AAATAATTGGTGGATAA

Upstream 100 bases:

>100_bases
TCACTTTTGATGAATTAAAAAAGAAAGTTGAAATTGAACTTAATGAGGAAGATGCCCTTACTTGGATTCGGGCTTTATAC
CAGAAAGGCATTATTGAGTA

Downstream 100 bases:

>100_bases
AATGGCAGATAATGATTTGATTAGAAACAAAGGAATAATGAGTGCTGCACAAACCATGTGGACGATAGATTTCGATTCTG
AACAGTCTTTAGAAATCGGC

Product: transcriptional regulatory protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MDEMLVEKYTDEENHIVYKKCKAFTNGKKYFLLNELGYSILIDHELFNMLQQKKVSTDFASKLLQHRLAEFDNLNHCDLC
TNYKIRPEFFMIDLTTRCNLNCKYCFRDVNNPENYKSISKEMLNEICKYIFDYCTKHEVKKISIQPWGGEPLLEMEKIKW
MYDFFENTPISVHFTIETNGVLLTEENVKWLFEHNVSIGISIDGNKEIHDRQRVFANTKGSFEYVLQGIKNVKKYYGDNF
GIINTITRNSIEHIEDILDFYAKDINIKHVKFNFVHESEFAANLCLNKEQVIDGTKRVYKKIMSLNNEGYNIIDMNIRHK
LLNLLIRDNSDICVSQGCTGGTSMIVFDMEGYIYPCELTDYIEERIGNVKEAVDLIKIVKEAKETKAYFKEKDDVSCLSC
SWRYFCKGGCSIKAKLSRTKKDDLECTINSTLYPEMIESILSEQKLINDFLNMKIIGG

Sequences:

>Translated_458_residues
MDEMLVEKYTDEENHIVYKKCKAFTNGKKYFLLNELGYSILIDHELFNMLQQKKVSTDFASKLLQHRLAEFDNLNHCDLC
TNYKIRPEFFMIDLTTRCNLNCKYCFRDVNNPENYKSISKEMLNEICKYIFDYCTKHEVKKISIQPWGGEPLLEMEKIKW
MYDFFENTPISVHFTIETNGVLLTEENVKWLFEHNVSIGISIDGNKEIHDRQRVFANTKGSFEYVLQGIKNVKKYYGDNF
GIINTITRNSIEHIEDILDFYAKDINIKHVKFNFVHESEFAANLCLNKEQVIDGTKRVYKKIMSLNNEGYNIIDMNIRHK
LLNLLIRDNSDICVSQGCTGGTSMIVFDMEGYIYPCELTDYIEERIGNVKEAVDLIKIVKEAKETKAYFKEKDDVSCLSC
SWRYFCKGGCSIKAKLSRTKKDDLECTINSTLYPEMIESILSEQKLINDFLNMKIIGG
>Mature_458_residues
MDEMLVEKYTDEENHIVYKKCKAFTNGKKYFLLNELGYSILIDHELFNMLQQKKVSTDFASKLLQHRLAEFDNLNHCDLC
TNYKIRPEFFMIDLTTRCNLNCKYCFRDVNNPENYKSISKEMLNEICKYIFDYCTKHEVKKISIQPWGGEPLLEMEKIKW
MYDFFENTPISVHFTIETNGVLLTEENVKWLFEHNVSIGISIDGNKEIHDRQRVFANTKGSFEYVLQGIKNVKKYYGDNF
GIINTITRNSIEHIEDILDFYAKDINIKHVKFNFVHESEFAANLCLNKEQVIDGTKRVYKKIMSLNNEGYNIIDMNIRHK
LLNLLIRDNSDICVSQGCTGGTSMIVFDMEGYIYPCELTDYIEERIGNVKEAVDLIKIVKEAKETKAYFKEKDDVSCLSC
SWRYFCKGGCSIKAKLSRTKKDDLECTINSTLYPEMIESILSEQKLINDFLNMKIIGG

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemical mechanism initiated via the reduc

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=371, Percent_Identity=24.7978436657682, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI48994973, Length=346, Percent_Identity=24.5664739884393, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53524; Mature: 53524

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEMLVEKYTDEENHIVYKKCKAFTNGKKYFLLNELGYSILIDHELFNMLQQKKVSTDFA
CCCCHHHHHCCCCCCEEHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHHHCCHHHH
SKLLQHRLAEFDNLNHCDLCTNYKIRPEFFMIDLTTRCNLNCKYCFRDVNNPENYKSISK
HHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHH
EMLNEICKYIFDYCTKHEVKKISIQPWGGEPLLEMEKIKWMYDFFENTPISVHFTIETNG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC
VLLTEENVKWLFEHNVSIGISIDGNKEIHDRQRVFANTKGSFEYVLQGIKNVKKYYGDNF
EEEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
GIINTITRNSIEHIEDILDFYAKDINIKHVKFNFVHESEFAANLCLNKEQVIDGTKRVYK
CEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
KIMSLNNEGYNIIDMNIRHKLLNLLIRDNSDICVSQGCTGGTSMIVFDMEGYIYPCELTD
HHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCEEEECHHHH
YIEERIGNVKEAVDLIKIVKEAKETKAYFKEKDDVSCLSCSWRYFCKGGCSIKAKLSRTK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHEECCCCEEEEECCCCC
KDDLECTINSTLYPEMIESILSEQKLINDFLNMKIIGG
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MDEMLVEKYTDEENHIVYKKCKAFTNGKKYFLLNELGYSILIDHELFNMLQQKKVSTDFA
CCCCHHHHHCCCCCCEEHHHHHHHCCCCEEEEEECCCCEEEECHHHHHHHHHHHCCHHHH
SKLLQHRLAEFDNLNHCDLCTNYKIRPEFFMIDLTTRCNLNCKYCFRDVNNPENYKSISK
HHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHH
EMLNEICKYIFDYCTKHEVKKISIQPWGGEPLLEMEKIKWMYDFFENTPISVHFTIETNG
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCC
VLLTEENVKWLFEHNVSIGISIDGNKEIHDRQRVFANTKGSFEYVLQGIKNVKKYYGDNF
EEEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
GIINTITRNSIEHIEDILDFYAKDINIKHVKFNFVHESEFAANLCLNKEQVIDGTKRVYK
CEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHCCHHHHHHHHHHHHH
KIMSLNNEGYNIIDMNIRHKLLNLLIRDNSDICVSQGCTGGTSMIVFDMEGYIYPCELTD
HHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEECCCEEEECHHHH
YIEERIGNVKEAVDLIKIVKEAKETKAYFKEKDDVSCLSCSWRYFCKGGCSIKAKLSRTK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHEECCCCEEEEECCCCC
KDDLECTINSTLYPEMIESILSEQKLINDFLNMKIIGG
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA