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The map label for this gene is carA [H]

Identifier: 238923138

GI number: 238923138

Start: 672770

End: 673840

Strand: Direct

Name: carA [H]

Synonym: EUBREC_0731

Alternate gene names: 238923138

Gene position: 672770-673840 (Clockwise)

Preceding gene: 238923137

Following gene: 238923139

Centisome position: 19.5

GC content: 43.23

Gene sequence:

>1071_bases
ATGAAAGCATTTTTAATTCTTGAAGATGGTAATGTATTTACCGGAACCAGCATTGGTTCAACAAGGGAAGTCATTAGTGA
GATAGTATTTAACACATCAATGACAGGATATCTGGAGGTACTTACAGATCCTTCCTATGCAGGACAGGCTGTTGTAATGA
CTTATCCACTTATCGGAAATTATGGAATCACACCGGATATGGAGTCGGAAAGACCATGGCCGGATGGATATATAGTAAGA
GAGCTGTCACGTATGCCAAGTAATTTCCGCTGCGAGGGTACTATACAGGATTTCCTTGAGAAAAACGATATTCCGGGAGT
GGCCGGCATTGATACACGTGCGCTTACAAAGATTCTCAGGGAAAAGGGTACTATGAACGGAATGATTACCACAAATGAGA
ATTACAATCTCGATGAGATTATTCCAAAGCTCAAGGCATATACTACAGGCAATGTGGTTGACAAGGTCACATGCACTGAG
AAAAAGGTTTTAAAGGGCCAAGGAAAGAGGGTTGCCCTCATGGATTTCGGTGCCAAAAACAACATCGCCAAATCACTCAA
TGAAAGAGGCTGTGAAGTTACAATTTACCCTGCACACACAACCGCTGAAGAGATACTTGGTGACAATCCTGACGGAATCA
TGCTCTCAAACGGACCTGGAGATCCAAAGGAGTGTGTAGATATTATAAAGGAAATAAAGAAGCTTTATGACAGCAATGTG
CCGATTTTTGCAATCTGCCTCGGACATCAGCTCATGGCACTTGCAACAGGCGCAGATACACACAAGATGAAGTACGGACA
CAGAGGTGGAAACCATCCGGTTAAGGATCTTGCGACAGGCCGTGTTTATATTTCTTCCCAGAACCATGGATATGTGGTTG
ACACAGATAAGCTTGACCCTAATGTGGCTGAGCCTGCATTTATAAATGTCAACGACGGCACTAATGAGGGACTTAAGTAT
GTGAACAAGAATATCTTCACAGTTCAGTTCCATCCGGAGGCATGCCCTGGACCACAGGATTCAGCATATCTTTTTGACCG
CTTTATTAACATGATGGGAGGTAACCAGTAA

Upstream 100 bases:

>100_bases
AGAAAAAAGAGACGCCCTCTTTTTTTTTGCGCAGACGTAAGGTTAGTTGCAGCTAAGAAGCTTATGGTTACAAAGTATAA
CACGAAAGGGCAGGGTAAAG

Downstream 100 bases:

>100_bases
TGCCAAGAAATAATGATATTAAAAAAGTATTAGTTATCGGTTCCGGTCCAATCGTAATCGGACAGGCCGCAGAGTTTGAC
TATGCAGGAACACAGGCCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKAFLILEDGNVFTGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGNYGITPDMESERPWPDGYIVR
ELSRMPSNFRCEGTIQDFLEKNDIPGVAGIDTRALTKILREKGTMNGMITTNENYNLDEIIPKLKAYTTGNVVDKVTCTE
KKVLKGQGKRVALMDFGAKNNIAKSLNERGCEVTIYPAHTTAEEILGDNPDGIMLSNGPGDPKECVDIIKEIKKLYDSNV
PIFAICLGHQLMALATGADTHKMKYGHRGGNHPVKDLATGRVYISSQNHGYVVDTDKLDPNVAEPAFINVNDGTNEGLKY
VNKNIFTVQFHPEACPGPQDSAYLFDRFINMMGGNQ

Sequences:

>Translated_356_residues
MKAFLILEDGNVFTGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGNYGITPDMESERPWPDGYIVR
ELSRMPSNFRCEGTIQDFLEKNDIPGVAGIDTRALTKILREKGTMNGMITTNENYNLDEIIPKLKAYTTGNVVDKVTCTE
KKVLKGQGKRVALMDFGAKNNIAKSLNERGCEVTIYPAHTTAEEILGDNPDGIMLSNGPGDPKECVDIIKEIKKLYDSNV
PIFAICLGHQLMALATGADTHKMKYGHRGGNHPVKDLATGRVYISSQNHGYVVDTDKLDPNVAEPAFINVNDGTNEGLKY
VNKNIFTVQFHPEACPGPQDSAYLFDRFINMMGGNQ
>Mature_356_residues
MKAFLILEDGNVFTGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGNYGITPDMESERPWPDGYIVR
ELSRMPSNFRCEGTIQDFLEKNDIPGVAGIDTRALTKILREKGTMNGMITTNENYNLDEIIPKLKAYTTGNVVDKVTCTE
KKVLKGQGKRVALMDFGAKNNIAKSLNERGCEVTIYPAHTTAEEILGDNPDGIMLSNGPGDPKECVDIIKEIKKLYDSNV
PIFAICLGHQLMALATGADTHKMKYGHRGGNHPVKDLATGRVYISSQNHGYVVDTDKLDPNVAEPAFINVNDGTNEGLKY
VNKNIFTVQFHPEACPGPQDSAYLFDRFINMMGGNQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=363, Percent_Identity=40.495867768595, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI21361331, Length=364, Percent_Identity=36.5384615384615, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI169790915, Length=364, Percent_Identity=36.5384615384615, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786215, Length=371, Percent_Identity=45.5525606469003, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=42.2343324250681, Blast_Score=278, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6324878, Length=369, Percent_Identity=40.650406504065, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=36.9230769230769, Blast_Score=251, Evalue=8e-68,
Organism=Drosophila melanogaster, GI45555749, Length=371, Percent_Identity=41.7789757412399, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642586, Length=371, Percent_Identity=41.7789757412399, Blast_Score=271, Evalue=6e-73,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39147; Mature: 39147

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLILEDGNVFTGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGN
CEEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEECC
YGITPDMESERPWPDGYIVRELSRMPSNFRCEGTIQDFLEKNDIPGVAGIDTRALTKILR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCCCCCCCCHHHHHHHHH
EKGTMNGMITTNENYNLDEIIPKLKAYTTGNVVDKVTCTEKKVLKGQGKRVALMDFGAKN
HCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCEEEEEECCCCC
NIAKSLNERGCEVTIYPAHTTAEEILGDNPDGIMLSNGPGDPKECVDIIKEIKKLYDSNV
HHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
PIFAICLGHQLMALATGADTHKMKYGHRGGNHPVKDLATGRVYISSQNHGYVVDTDKLDP
CEEEEEHHHHHEEHCCCCCHHHEECCCCCCCCCHHHHCCCEEEEECCCCCEEEECCCCCC
NVAEPAFINVNDGTNEGLKYVNKNIFTVQFHPEACPGPQDSAYLFDRFINMMGGNQ
CCCCCEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAFLILEDGNVFTGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVVMTYPLIGN
CEEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEECC
YGITPDMESERPWPDGYIVRELSRMPSNFRCEGTIQDFLEKNDIPGVAGIDTRALTKILR
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEECCHHHHHHHHCCCCCCCCCCHHHHHHHHH
EKGTMNGMITTNENYNLDEIIPKLKAYTTGNVVDKVTCTEKKVLKGQGKRVALMDFGAKN
HCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCEEEEEECCCCC
NIAKSLNERGCEVTIYPAHTTAEEILGDNPDGIMLSNGPGDPKECVDIIKEIKKLYDSNV
HHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
PIFAICLGHQLMALATGADTHKMKYGHRGGNHPVKDLATGRVYISSQNHGYVVDTDKLDP
CEEEEEHHHHHEEHCCCCCHHHEECCCCCCCCCHHHHCCCEEEEECCCCCEEEECCCCCC
NVAEPAFINVNDGTNEGLKYVNKNIFTVQFHPEACPGPQDSAYLFDRFINMMGGNQ
CCCCCEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]