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The map label for this gene is ptsI [H]

Identifier: 238922770

GI number: 238922770

Start: 323539

End: 325239

Strand: Direct

Name: ptsI [H]

Synonym: EUBREC_0358

Alternate gene names: 238922770

Gene position: 323539-325239 (Clockwise)

Preceding gene: 238922769

Following gene: 238922771

Centisome position: 9.38

GC content: 48.09

Gene sequence:

>1701_bases
ATGTACAAAGGAATCGCCGGCTCAGAAGGAATCGGAATCGGAAATGTGGTCATTATCGAAGAGCATGAGATAGTGATTGA
GAATAAGAAAATTACAGATACAGACGCTGAAATCGCACGTCTCCAGGGAGCCATTGAGAAATTTGTAAATGATACAAACG
CCATGGCAGACAGAATGGAAAAGACAGTCGGAGCAAAGGATGCGGATATCCTTCGCGGACATATCCAGATGCTTCAGGAT
CCGACCATAGAGGAGCAGATTACAGCGCTCATAGTAAGTGAGAAAATCACGGCAGAGAAGGCGCTCGACCAGGTGCTTGA
GCAGACAGCAGAGATATTTGCACAGATACCTGATGAGCTTTTGCAGCAGAGAGCAACAGATTTCAGGGATATCAAAGCCA
GAATCCTTAAGATTCTGCTTGGAATCGAGGACTATGATATCTCAGCAGCTCCGGCAGGCACAGTGCTCGTGGCACATGAC
CTGACACCATCCATGACAGCAGGTATCGTGGCAGAGAATATCGCAGGAATACTCACAGAGGTGGGCGGCAGAACCTCCCA
CTCAGCAATACTTGCAAGAGCAATGGAAATCCCGGCAGTTCTCAGTATTGATGGAATCTGCACAGCTGTCAAAAATGGTG
ACAGAGTGGTACTCAACGGAACAAGCGGAGAGGCAATCGTAAACCCTGACGCTGAGACAGAGCAGAAATTCGCTGGGTTG
CTTGAGGAATACAAAAAAGAGAAAGAGCTCTTAAAGAAATTTGCGGGTGTGCCGACAGTATCAGCAGACGGCACAAAGGT
AGAGCTTGTATGCAATATCGGAAAGCCTGAGGACGCAAAGAAAGCTGTAGAGTGTGACGGAGAGGGAATAGGACTTTTCC
GTACAGAGTTCCTTTTCATGGACAGAGACACAATTCCTACAGAGGAAGAGCAGTTTGAGGCATACAAATCAGTAGCAGAG
ACAATGAAGGGCAAGCCTGTCATTATCCGTACACTCGATATCGGAGGTGACAAGGAAATCCCATATCTGGGTCTTGAGAA
AGAGGACAACCCATTCCTAGGCTACAGAGCTATCAGATTCTGTCTCCAGAGAACAGACATCTACAATACACAGCTTCGTG
CACTTGTTAGAGCCAGTGCATTCGGCAGGATAAAGATAATGGTTCCGCTTGTGACATGCGTAGATGAGCTTCGCAGTGTA
AAAGCAATGGTAGCTGACATCATGAAAGAGCTTGATGCAGAGGGCATTGCATACAACAAGAACCTCGAAATTGGTATCAT
GATGGAGACACCGGCAGCCTGCATGATGGCAGATGTGCTTGCAAAAGAGGCAGCCTTCTTCAGTATCGGAACAAACGACC
TGACAGGCTACACAATGGCAGTAGACCGTGGAAACTCAAAGGTAGCATACCTTTATTCAGCCTTCAATCCGGCAGTGCTT
CGCGCAATCAAGCGTATCATCGAGTGCGGTAAGAAAGAGGGAATCATGGTAGGCATGTGTGGAGAGGCAGCAGCAGATCC
AAAGCTCATTCCGCTGTTACTCGCATTCGGACTTGATGAGTTCTCAGTGAGTGCCACAAGTGTGCTCAAGACCAGAAAGA
CCATCGCAGACAGCAGCATGGCAGAATGCAAGGCATTGGCAGACAAGGTAATGGCATGCGCTACAGAGGCAGAGGTACAG
GCAGTGCTTGCACAGCAGTAG

Upstream 100 bases:

>100_bases
TGAGGGAGCTGACGAGGAAGCAGCACTTGCAAAGATGGAAGAGCTCGAGGCAGACAACTTCGGAGATTAATCAACCAGCA
AAGTCAATCGGAGGTGTGAT

Downstream 100 bases:

>100_bases
ATGCTAAATTTAAACAAGTATAAAAGTTAGTAAAAAAGTATAAATAAGAGTAAAATATAAAAGTATAACATTGAAATTTA
TTATAAAGACGGCTGAACCG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MYKGIAGSEGIGIGNVVIIEEHEIVIENKKITDTDAEIARLQGAIEKFVNDTNAMADRMEKTVGAKDADILRGHIQMLQD
PTIEEQITALIVSEKITAEKALDQVLEQTAEIFAQIPDELLQQRATDFRDIKARILKILLGIEDYDISAAPAGTVLVAHD
LTPSMTAGIVAENIAGILTEVGGRTSHSAILARAMEIPAVLSIDGICTAVKNGDRVVLNGTSGEAIVNPDAETEQKFAGL
LEEYKKEKELLKKFAGVPTVSADGTKVELVCNIGKPEDAKKAVECDGEGIGLFRTEFLFMDRDTIPTEEEQFEAYKSVAE
TMKGKPVIIRTLDIGGDKEIPYLGLEKEDNPFLGYRAIRFCLQRTDIYNTQLRALVRASAFGRIKIMVPLVTCVDELRSV
KAMVADIMKELDAEGIAYNKNLEIGIMMETPAACMMADVLAKEAAFFSIGTNDLTGYTMAVDRGNSKVAYLYSAFNPAVL
RAIKRIIECGKKEGIMVGMCGEAAADPKLIPLLLAFGLDEFSVSATSVLKTRKTIADSSMAECKALADKVMACATEAEVQ
AVLAQQ

Sequences:

>Translated_566_residues
MYKGIAGSEGIGIGNVVIIEEHEIVIENKKITDTDAEIARLQGAIEKFVNDTNAMADRMEKTVGAKDADILRGHIQMLQD
PTIEEQITALIVSEKITAEKALDQVLEQTAEIFAQIPDELLQQRATDFRDIKARILKILLGIEDYDISAAPAGTVLVAHD
LTPSMTAGIVAENIAGILTEVGGRTSHSAILARAMEIPAVLSIDGICTAVKNGDRVVLNGTSGEAIVNPDAETEQKFAGL
LEEYKKEKELLKKFAGVPTVSADGTKVELVCNIGKPEDAKKAVECDGEGIGLFRTEFLFMDRDTIPTEEEQFEAYKSVAE
TMKGKPVIIRTLDIGGDKEIPYLGLEKEDNPFLGYRAIRFCLQRTDIYNTQLRALVRASAFGRIKIMVPLVTCVDELRSV
KAMVADIMKELDAEGIAYNKNLEIGIMMETPAACMMADVLAKEAAFFSIGTNDLTGYTMAVDRGNSKVAYLYSAFNPAVL
RAIKRIIECGKKEGIMVGMCGEAAADPKLIPLLLAFGLDEFSVSATSVLKTRKTIADSSMAECKALADKVMACATEAEVQ
AVLAQQ
>Mature_566_residues
MYKGIAGSEGIGIGNVVIIEEHEIVIENKKITDTDAEIARLQGAIEKFVNDTNAMADRMEKTVGAKDADILRGHIQMLQD
PTIEEQITALIVSEKITAEKALDQVLEQTAEIFAQIPDELLQQRATDFRDIKARILKILLGIEDYDISAAPAGTVLVAHD
LTPSMTAGIVAENIAGILTEVGGRTSHSAILARAMEIPAVLSIDGICTAVKNGDRVVLNGTSGEAIVNPDAETEQKFAGL
LEEYKKEKELLKKFAGVPTVSADGTKVELVCNIGKPEDAKKAVECDGEGIGLFRTEFLFMDRDTIPTEEEQFEAYKSVAE
TMKGKPVIIRTLDIGGDKEIPYLGLEKEDNPFLGYRAIRFCLQRTDIYNTQLRALVRASAFGRIKIMVPLVTCVDELRSV
KAMVADIMKELDAEGIAYNKNLEIGIMMETPAACMMADVLAKEAAFFSIGTNDLTGYTMAVDRGNSKVAYLYSAFNPAVL
RAIKRIIECGKKEGIMVGMCGEAAADPKLIPLLLAFGLDEFSVSATSVLKTRKTIADSSMAECKALADKVMACATEAEVQ
AVLAQQ

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=565, Percent_Identity=43.5398230088496, Blast_Score=477, Evalue=1e-135,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=33.2161687170475, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI48994992, Length=511, Percent_Identity=34.6379647749511, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789193, Length=563, Percent_Identity=31.7939609236234, Blast_Score=289, Evalue=4e-79,
Organism=Escherichia coli, GI1787994, Length=427, Percent_Identity=25.9953161592506, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI226510935, Length=173, Percent_Identity=30.0578034682081, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61329; Mature: 61329

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKGIAGSEGIGIGNVVIIEEHEIVIENKKITDTDAEIARLQGAIEKFVNDTNAMADRME
CCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
KTVGAKDADILRGHIQMLQDPTIEEQITALIVSEKITAEKALDQVLEQTAEIFAQIPDEL
HHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LQQRATDFRDIKARILKILLGIEDYDISAAPAGTVLVAHDLTPSMTAGIVAENIAGILTE
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VGGRTSHSAILARAMEIPAVLSIDGICTAVKNGDRVVLNGTSGEAIVNPDAETEQKFAGL
HCCCCHHHHHHHHHHHCCEEEEECHHHHHCCCCCEEEEECCCCCEEECCCCHHHHHHHHH
LEEYKKEKELLKKFAGVPTVSADGTKVELVCNIGKPEDAKKAVECDGEGIGLFRTEFLFM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEEE
DRDTIPTEEEQFEAYKSVAETMKGKPVIIRTLDIGGDKEIPYLGLEKEDNPFLGYRAIRF
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHH
CLQRTDIYNTQLRALVRASAFGRIKIMVPLVTCVDELRSVKAMVADIMKELDAEGIAYNK
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
NLEIGIMMETPAACMMADVLAKEAAFFSIGTNDLTGYTMAVDRGNSKVAYLYSAFNPAVL
CCEEEEEECCCHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCEEEEEEECCCHHHH
RAIKRIIECGKKEGIMVGMCGEAAADPKLIPLLLAFGLDEFSVSATSVLKTRKTIADSSM
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCHH
AECKALADKVMACATEAEVQAVLAQQ
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYKGIAGSEGIGIGNVVIIEEHEIVIENKKITDTDAEIARLQGAIEKFVNDTNAMADRME
CCCCCCCCCCCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHH
KTVGAKDADILRGHIQMLQDPTIEEQITALIVSEKITAEKALDQVLEQTAEIFAQIPDEL
HHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LQQRATDFRDIKARILKILLGIEDYDISAAPAGTVLVAHDLTPSMTAGIVAENIAGILTE
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VGGRTSHSAILARAMEIPAVLSIDGICTAVKNGDRVVLNGTSGEAIVNPDAETEQKFAGL
HCCCCHHHHHHHHHHHCCEEEEECHHHHHCCCCCEEEEECCCCCEEECCCCHHHHHHHHH
LEEYKKEKELLKKFAGVPTVSADGTKVELVCNIGKPEDAKKAVECDGEGIGLFRTEFLFM
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEEE
DRDTIPTEEEQFEAYKSVAETMKGKPVIIRTLDIGGDKEIPYLGLEKEDNPFLGYRAIRF
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHH
CLQRTDIYNTQLRALVRASAFGRIKIMVPLVTCVDELRSVKAMVADIMKELDAEGIAYNK
HHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
NLEIGIMMETPAACMMADVLAKEAAFFSIGTNDLTGYTMAVDRGNSKVAYLYSAFNPAVL
CCEEEEEECCCHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECCCCEEEEEEECCCHHHH
RAIKRIIECGKKEGIMVGMCGEAAADPKLIPLLLAFGLDEFSVSATSVLKTRKTIADSSM
HHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCHH
AECKALADKVMACATEAEVQAVLAQQ
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1551842; 1901791 [H]