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The map label for this gene is yiaE [C]

Identifier: 238922765

GI number: 238922765

Start: 315732

End: 316667

Strand: Direct

Name: yiaE [C]

Synonym: EUBREC_0353

Alternate gene names: 238922765

Gene position: 315732-316667 (Clockwise)

Preceding gene: 238922764

Following gene: 238922766

Centisome position: 9.15

GC content: 44.34

Gene sequence:

>936_bases
ATGAAAATAGTATTTTTAGATTCCAAAACAATTGGTGACGACATCGACCTTTCCGAATATGACAAGCTCGGAGAGGTTGT
CAAATATGATTTTTCAACAACAGAGGAAGCAGCGGAGCGCACAAGGGATGCTGATGTGATTGTTTTAAATAAGGTCGAGG
TAAATGAGAAATCAATAGGACAGGCAAAAAATTTAAAGCTTGTATGTGTGACTGCAACAGGCACAAACAATCTGGATAAA
GAATATCTGGTAAAAAGAGGCATTGAATGGAGAAATGTCGCAGGGTATTCTACAGAGACCGTGGCGCAGCATACGTTTGC
ACTGCTATTTTACCTGCTTGAGAAGCTGAGATATTATGATGATTATGTGAAGAGCGAGAAATATGTGGGTGATACGTCGT
TTACGCATTTTTCCAATGTGTTTCACCAGATTAGCGGTATGACCTGGGGCATCGTGGGGCTTGGAAATATCGGAAGACGC
GTGGCTGATATCGCAAAGGCTTTTGGCTGCCATGTGGTTTATTATTCTACCTCAGGAAGAAACTCGCAGCCGGGCTATGA
GAGGGTGGATTTTGATACTCTGCTTGCCACGTCTGATATCGTGTCGGTGCATGCACCGCTCACAGATGATACACTTGGTC
TGATGGACAAGGCTGCGTTTGAAAAGATGAAAAAATCTGCGATATTTTTAAATCTTGGCAGAGGTCCTATAGTGAATGAA
GCGGATCTGGCACAGGCGCTTATGGATGGAGAGCTGGCGGCGGCAGGTCTTGATGTGCTGGCGCAGGAGCCTATGAGTGA
GGACAATCCGCTCCGTGCGATAAAGGATTCAAACAAGCTGATTATCACACCGCATATTGCGTGGGCAAGCGTTGAGGCAA
GAACAAATCTGATGCACATTATATACAGTCAAATTGAGGACTTCTTTGCTAATTAG

Upstream 100 bases:

>100_bases
AATATACCATATGCCGGCGCACCATCACAGCCTGCAAGTGCGAAGTCCCCTAAAAATATAAATTAAAACAAATAAATATA
TAGCACAGAGGAAACAAACC

Downstream 100 bases:

>100_bases
GAAATATAATTTAAAATCAAATCGCAACATTAAGAGAAAGCACTAAGAGAAAGCATTAAGAGGCAATATTAACAGGCAAT
ATGAAGAGGCAGAAATGAGC

Product: 2-hydroxyacid dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKIVFLDSKTIGDDIDLSEYDKLGEVVKYDFSTTEEAAERTRDADVIVLNKVEVNEKSIGQAKNLKLVCVTATGTNNLDK
EYLVKRGIEWRNVAGYSTETVAQHTFALLFYLLEKLRYYDDYVKSEKYVGDTSFTHFSNVFHQISGMTWGIVGLGNIGRR
VADIAKAFGCHVVYYSTSGRNSQPGYERVDFDTLLATSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNE
ADLAQALMDGELAAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHIIYSQIEDFFAN

Sequences:

>Translated_311_residues
MKIVFLDSKTIGDDIDLSEYDKLGEVVKYDFSTTEEAAERTRDADVIVLNKVEVNEKSIGQAKNLKLVCVTATGTNNLDK
EYLVKRGIEWRNVAGYSTETVAQHTFALLFYLLEKLRYYDDYVKSEKYVGDTSFTHFSNVFHQISGMTWGIVGLGNIGRR
VADIAKAFGCHVVYYSTSGRNSQPGYERVDFDTLLATSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNE
ADLAQALMDGELAAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHIIYSQIEDFFAN
>Mature_311_residues
MKIVFLDSKTIGDDIDLSEYDKLGEVVKYDFSTTEEAAERTRDADVIVLNKVEVNEKSIGQAKNLKLVCVTATGTNNLDK
EYLVKRGIEWRNVAGYSTETVAQHTFALLFYLLEKLRYYDDYVKSEKYVGDTSFTHFSNVFHQISGMTWGIVGLGNIGRR
VADIAKAFGCHVVYYSTSGRNSQPGYERVDFDTLLATSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNE
ADLAQALMDGELAAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHIIYSQIEDFFAN

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=247, Percent_Identity=34.8178137651822, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI23308577, Length=286, Percent_Identity=28.6713286713287, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI61743967, Length=325, Percent_Identity=28.3076923076923, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4557497, Length=325, Percent_Identity=28.3076923076923, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI145580575, Length=291, Percent_Identity=28.5223367697594, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI145580578, Length=325, Percent_Identity=27.6923076923077, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI4557499, Length=325, Percent_Identity=27.6923076923077, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI87082289, Length=302, Percent_Identity=33.112582781457, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787645, Length=290, Percent_Identity=24.8275862068966, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789279, Length=266, Percent_Identity=26.6917293233083, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081824, Length=180, Percent_Identity=30, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25147481, Length=293, Percent_Identity=26.2798634812287, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=28.5140562248996, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=317, Percent_Identity=27.1293375394322, Blast_Score=108, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=281, Percent_Identity=27.4021352313167, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=309, Percent_Identity=28.4789644012945, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324964, Length=307, Percent_Identity=26.7100977198697, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=127, Percent_Identity=31.496062992126, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=288, Percent_Identity=32.9861111111111, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24646446, Length=311, Percent_Identity=29.903536977492, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646448, Length=311, Percent_Identity=29.903536977492, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646452, Length=311, Percent_Identity=29.903536977492, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24646450, Length=311, Percent_Identity=29.903536977492, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472511, Length=311, Percent_Identity=29.903536977492, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585514, Length=273, Percent_Identity=29.6703296703297, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574282, Length=273, Percent_Identity=29.6703296703297, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45552429, Length=273, Percent_Identity=29.6703296703297, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574284, Length=276, Percent_Identity=30.7971014492754, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45551003, Length=276, Percent_Identity=30.7971014492754, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585516, Length=235, Percent_Identity=29.3617021276596, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571528, Length=249, Percent_Identity=29.718875502008, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19921140, Length=250, Percent_Identity=24.4, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 34616; Mature: 34616

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFLDSKTIGDDIDLSEYDKLGEVVKYDFSTTEEAAERTRDADVIVLNKVEVNEKSIG
CEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEEECCHHHCC
QAKNLKLVCVTATGTNNLDKEYLVKRGIEWRNVAGYSTETVAQHTFALLFYLLEKLRYYD
CCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DYVKSEKYVGDTSFTHFSNVFHQISGMTWGIVGLGNIGRRVADIAKAFGCHVVYYSTSGR
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCC
NSQPGYERVDFDTLLATSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNE
CCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCH
ADLAQALMDGELAAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHI
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCEEEEHHHHHHHHHH
IYSQIEDFFAN
HHHHHHHHHCC
>Mature Secondary Structure
MKIVFLDSKTIGDDIDLSEYDKLGEVVKYDFSTTEEAAERTRDADVIVLNKVEVNEKSIG
CEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEEECCHHHCC
QAKNLKLVCVTATGTNNLDKEYLVKRGIEWRNVAGYSTETVAQHTFALLFYLLEKLRYYD
CCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DYVKSEKYVGDTSFTHFSNVFHQISGMTWGIVGLGNIGRRVADIAKAFGCHVVYYSTSGR
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCC
NSQPGYERVDFDTLLATSDIVSVHAPLTDDTLGLMDKAAFEKMKKSAIFLNLGRGPIVNE
CCCCCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCH
ADLAQALMDGELAAAGLDVLAQEPMSEDNPLRAIKDSNKLIITPHIAWASVEARTNLMHI
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCEEEEHHHHHHHHHH
IYSQIEDFFAN
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]