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The map label for this gene is pfkA3 [H]

Identifier: 238922751

GI number: 238922751

Start: 297856

End: 298950

Strand: Direct

Name: pfkA3 [H]

Synonym: EUBREC_0327

Alternate gene names: 238922751

Gene position: 297856-298950 (Clockwise)

Preceding gene: 238922750

Following gene: 238922752

Centisome position: 8.63

GC content: 49.5

Gene sequence:

>1095_bases
ATGCATAAGGAGGAAGCAGCAATGAAGAAAATTGGAATGCTTACCAGCGGAGGAGACTGCCAGGCACTTAACGCAGCCAT
GAGAGGTGTGGTAAAGGCGCTGGCACACAATATCGACGAGCTTGAGATTTACGGCTTTGAGAACGGGTACAAGGGACTCA
TATATGAGAACTACAGAATACTCACATCAAAGGATTTTTCGGGCATCCTGACAAAGGGCGGCACAATACTCGGCTCATCA
AGACAGCCTTTTAAGCTTATGAGAGTGCCGGATGAGAACGGACTGGACAAGGTTGCAGCCATGAAAAACACATACAAGAA
GCTTGGTCTTGACTGTCTCGTTATTCTGGGCGGAAACGGCACACAAAAGACAGCCAATCTTCTCAGGGAAGAGGGACTTA
ATATCATTCATCTGCCAAAGACAATCGACAATGATATCTACGGCACAGATGTGACCTTTGGCTTCCAGAGCGCGATCAAT
ATAGCTACAGATGCAATCGACTGCATCCACACCACAGCAGCATCCCACAACCGTGTATTTATCGTTGAGGTTATGGGCCA
CAAGGTAGGCTGGCTCACACTGTACGCAGGTGTTGCGGGCGGTGCAGACATCATTCTGCTTCCTGAGATACCATACGATA
TTGACAAGGTCGTAGATGCCATCCACAAGAGAACAAAGGAGGGCAAGGGCTTTACCATTCTTGCAGTAGCAGAGGGAGCC
ATCTCCAAGGAGGATGCAGCCCTTTCCAAAAAGGAATACAGGAAGAAGCTTGCAAAGAGCAAATACCCGTCAGTATCATA
CGAGGTTGCAGACCGCATCAGCGAAAGACTCGGTCTTGAGGTCAGAGTGGCAGTTCCGGGACATACACAGCGAGGCGGCA
GCCCATGTCCTTACGACAGAGTGCTTTGTACACGTCTTGGCTCAGCAGCAGCAAAGGCCATCATGGATGAGGATTACGGC
TGCATGATAGCCATGGTAAACGGACAGACAAAGAGGGTTCCGCTTGCCGACGTAGCAGGAAAGCTTAAGACTGTAGATCC
AAAGAGCCAGATGATCAAGGAAGCAAAGCGCACCGGCATCTGCTTCGGAGATTAA

Upstream 100 bases:

>100_bases
AGCGTATTTTCAGAAAGCTCATGAATGAGGAGGATAGGCTCCGCGAGGAGGAGAAGGATAAGGAGTACAGGGATTAGCAT
AATAAAAGACAATCATAATA

Downstream 100 bases:

>100_bases
GCAGCAATATTAGATATAAGGAAGGTGAAAAGGCGTGTCTTATCAGGCCCTATACCGTAAGTTCCGTCCACAGGAGTTCG
AGGACGTAAAGGGACAGGAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MHKEEAAMKKIGMLTSGGDCQALNAAMRGVVKALAHNIDELEIYGFENGYKGLIYENYRILTSKDFSGILTKGGTILGSS
RQPFKLMRVPDENGLDKVAAMKNTYKKLGLDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAIN
IATDAIDCIHTTAASHNRVFIVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIDKVVDAIHKRTKEGKGFTILAVAEGA
ISKEDAALSKKEYRKKLAKSKYPSVSYEVADRISERLGLEVRVAVPGHTQRGGSPCPYDRVLCTRLGSAAAKAIMDEDYG
CMIAMVNGQTKRVPLADVAGKLKTVDPKSQMIKEAKRTGICFGD

Sequences:

>Translated_364_residues
MHKEEAAMKKIGMLTSGGDCQALNAAMRGVVKALAHNIDELEIYGFENGYKGLIYENYRILTSKDFSGILTKGGTILGSS
RQPFKLMRVPDENGLDKVAAMKNTYKKLGLDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAIN
IATDAIDCIHTTAASHNRVFIVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIDKVVDAIHKRTKEGKGFTILAVAEGA
ISKEDAALSKKEYRKKLAKSKYPSVSYEVADRISERLGLEVRVAVPGHTQRGGSPCPYDRVLCTRLGSAAAKAIMDEDYG
CMIAMVNGQTKRVPLADVAGKLKTVDPKSQMIKEAKRTGICFGD
>Mature_364_residues
MHKEEAAMKKIGMLTSGGDCQALNAAMRGVVKALAHNIDELEIYGFENGYKGLIYENYRILTSKDFSGILTKGGTILGSS
RQPFKLMRVPDENGLDKVAAMKNTYKKLGLDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAIN
IATDAIDCIHTTAASHNRVFIVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIDKVVDAIHKRTKEGKGFTILAVAEGA
ISKEDAALSKKEYRKKLAKSKYPSVSYEVADRISERLGLEVRVAVPGHTQRGGSPCPYDRVLCTRLGSAAAKAIMDEDYG
CMIAMVNGQTKRVPLADVAGKLKTVDPKSQMIKEAKRTGICFGD

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=376, Percent_Identity=32.9787234042553, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI11321601, Length=364, Percent_Identity=32.1428571428571, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI266453768, Length=383, Percent_Identity=32.1148825065274, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI266453748, Length=383, Percent_Identity=32.1148825065274, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI4505749, Length=383, Percent_Identity=32.1148825065274, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI266453619, Length=383, Percent_Identity=32.1148825065274, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1790350, Length=336, Percent_Identity=37.202380952381, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI25147584, Length=378, Percent_Identity=31.2169312169312, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17558788, Length=345, Percent_Identity=30.1449275362319, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI72003601, Length=211, Percent_Identity=33.6492890995261, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=370, Percent_Identity=32.972972972973, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=370, Percent_Identity=31.3513513513514, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652337, Length=369, Percent_Identity=32.7913279132791, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI28573326, Length=369, Percent_Identity=32.520325203252, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17647809, Length=369, Percent_Identity=32.7913279132791, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 39456; Mature: 39456

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKEEAAMKKIGMLTSGGDCQALNAAMRGVVKALAHNIDELEIYGFENGYKGLIYENYRI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECEEE
LTSKDFSGILTKGGTILGSSRQPFKLMRVPDENGLDKVAAMKNTYKKLGLDCLVILGGNG
EECCCCCHHEECCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCC
TQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAINIATDAIDCIHTTAASHNRVF
CHHHHHHHHHCCCCEEECCCHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEE
IVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIDKVVDAIHKRTKEGKGFTILAVAEGA
EEEECCCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
ISKEDAALSKKEYRKKLAKSKYPSVSYEVADRISERLGLEVRVAVPGHTQRGGSPCPYDR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHH
VLCTRLGSAAAKAIMDEDYGCMIAMVNGQTKRVPLADVAGKLKTVDPKSQMIKEAKRTGI
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCC
CFGD
CCCC
>Mature Secondary Structure
MHKEEAAMKKIGMLTSGGDCQALNAAMRGVVKALAHNIDELEIYGFENGYKGLIYENYRI
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECEEE
LTSKDFSGILTKGGTILGSSRQPFKLMRVPDENGLDKVAAMKNTYKKLGLDCLVILGGNG
EECCCCCHHEECCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCC
TQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAINIATDAIDCIHTTAASHNRVF
CHHHHHHHHHCCCCEEECCCHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEE
IVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIDKVVDAIHKRTKEGKGFTILAVAEGA
EEEECCCCCEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCC
ISKEDAALSKKEYRKKLAKSKYPSVSYEVADRISERLGLEVRVAVPGHTQRGGSPCPYDR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHH
VLCTRLGSAAAKAIMDEDYGCMIAMVNGQTKRVPLADVAGKLKTVDPKSQMIKEAKRTGI
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCC
CFGD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]