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The map label for this gene is ywkD [H]

Identifier: 238922690

GI number: 238922690

Start: 253310

End: 253690

Strand: Direct

Name: ywkD [H]

Synonym: EUBREC_0265

Alternate gene names: 238922690

Gene position: 253310-253690 (Clockwise)

Preceding gene: 238922689

Following gene: 238922692

Centisome position: 7.34

GC content: 36.22

Gene sequence:

>381_bases
ATGAATTTAAAAAAAGTTCATCATATTGCCATTATTGGGAGCGACTATGAGAAATCAAAGCACTTTTATGTGGATCTGTT
AGGTTTTTCTATTATTCGAGAAAATTACAGACCGGAACGTGATGATTATAAAATTGATCTTCAACTCGATGAGATAGAAT
TAGAACTTTTTATCATTAAAAATTGTCCGAAGCGTCCAAGTTATCCAGAGGCTTATGGTCTCCGGCATCTTGCCTTTGCT
GTAGATTCGGTCGATGATACTGTAAGAGAATTGAATAAAAGGGGCATCATAACTGAGCCGATTAGACTCGATACTTACAC
AGGCAAAAGAATGACATTCTTTCATGATCCGGACAATCTACCCTTGGAAATTCACGAATAA

Upstream 100 bases:

>100_bases
AGAATACTTATAAGCTTTTGAGAAAATATATTACAAAATAGTATCATTGTGATACATTATTTCATGTATTCAAACAAAGT
AGGAGAGAGGTAAATACACC

Downstream 100 bases:

>100_bases
GGTATTGCAGTTAAAAGATGTGCCCTGTTTTTGGCTGTGATTTTATAAATTTAAATGTGTAGGGAACAGAAACTTTGAAT
TCTCACTTGAGGTATTTTTA

Product: glyoxalase I (glyoxalase family)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MNLKKVHHIAIIGSDYEKSKHFYVDLLGFSIIRENYRPERDDYKIDLQLDEIELELFIIKNCPKRPSYPEAYGLRHLAFA
VDSVDDTVRELNKRGIITEPIRLDTYTGKRMTFFHDPDNLPLEIHE

Sequences:

>Translated_126_residues
MNLKKVHHIAIIGSDYEKSKHFYVDLLGFSIIRENYRPERDDYKIDLQLDEIELELFIIKNCPKRPSYPEAYGLRHLAFA
VDSVDDTVRELNKRGIITEPIRLDTYTGKRMTFFHDPDNLPLEIHE
>Mature_126_residues
MNLKKVHHIAIIGSDYEKSKHFYVDLLGFSIIRENYRPERDDYKIDLQLDEIELELFIIKNCPKRPSYPEAYGLRHLAFA
VDSVDDTVRELNKRGIITEPIRLDTYTGKRMTFFHDPDNLPLEIHE

Specific function: Unknown

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081697, Length=127, Percent_Identity=54.3307086614173, Blast_Score=137, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: NA

Molecular weight: Translated: 14919; Mature: 14919

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKKVHHIAIIGSDYEKSKHFYVDLLGFSIIRENYRPERDDYKIDLQLDEIELELFIIK
CCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEEEEE
NCPKRPSYPEAYGLRHLAFAVDSVDDTVRELNKRGIITEPIRLDTYTGKRMTFFHDPDNL
CCCCCCCCCHHHCCEEEEHHHCCHHHHHHHHHHCCCEECCEEEECCCCCEEEEEECCCCC
PLEIHE
CEEEEC
>Mature Secondary Structure
MNLKKVHHIAIIGSDYEKSKHFYVDLLGFSIIRENYRPERDDYKIDLQLDEIELELFIIK
CCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEECCEEEEEEEEE
NCPKRPSYPEAYGLRHLAFAVDSVDDTVRELNKRGIITEPIRLDTYTGKRMTFFHDPDNL
CCCCCCCCCHHHCCEEEEHHHCCHHHHHHHHHHCCCEECCEEEECCCCCEEEEEECCCCC
PLEIHE
CEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]