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The map label for this gene is rafD [H]

Identifier: 238922682

GI number: 238922682

Start: 241745

End: 243208

Strand: Direct

Name: rafD [H]

Synonym: EUBREC_0257

Alternate gene names: 238922682

Gene position: 241745-243208 (Clockwise)

Preceding gene: 238922681

Following gene: 238922683

Centisome position: 7.01

GC content: 44.4

Gene sequence:

>1464_bases
ATGAGCGAATTATTGGAAAAAGCAAGAAGCTACGAGGCAAAAAAAATAGCTGCTACAGATAAAGAGAGTAAACCTTTGTT
TCATATCAGTGCACCGGCAGGATGGATTAATGATCCGAATGGATTTTCTGTCTATAACGGAAAAATTCATCTGTTTTATC
AGTACTATCCATATCTGCGTGAGTGGGGACCTATGCATTGGGGGCATAGTACGACAGGCGATATGATTAAATGGGAGCAG
CTTCCGTGTGCTCTGGCACCTGACGAGGAATATGATAAAAAAGGATGTTTCTCAGGAAGTGCCATTGAGGCAGATGGTAA
GCATGTGCTTGTATACACAGGTGTCACAAGGATTAAACTTCCTGACGGTAGTGAGCAGGAGAGACAGAACCAGTGCATCG
CATTTGGAGACGGACTTAATTATGTGAAGCATGAGAATAATCCTGTTGTGACAGGCGATATGCTTCCTGAAAACTGCAGC
AGAATCGATTTCCGTGACCCTAAAATATGGAAAGAGGATGACACATATTATCTTATAGTTGGAAGCAAGGACTTAAACAA
TGTGGGACAGGTAGTGCTCTGCTCTTCAAAAAACCTGACCGATTGGCAGTTTGAGACAATCCTTGCGGCAAACAGCACCG
GCAAAATAGGTACCATGTGGGAGTGCCCGGACTTCTTTGGGCTGGAGGATAAGCATGTGCTCATCTGTTCACCGCAGAGT
ATGAAGGCAGACAAATATGAGTTTCATAACGGACACAACTCTGTATATTTTCTGGGGGATTATGATAAAGAAAATCATGT
ATTTATCAAGGAAGAGCCGCACACTCTTGACTATGGCATGGACTTTTATGCGCCACAGACTACACTGCTTCCGGACGGAC
GCCGTGTGATGATTGCCTGGATGAAGTCATGGGATGCATGTGTCGTTCCTGATTCGCAGGATTGGCAGGGCATGATGACA
CTGCCTCGTGAGCTTGAAATAAAGGACGGCCGCATCTGGCAGAAGCCGGTTAAGGAAATAGAAAATTATCGTGCAAACAA
GTGTCATTATACAGATAAAAAAATAGACTGCTACACGACCTTTGATGGCATAAAGGGCCGCACTCTCGACATGACAGTGG
AGCTTTCAGGCGAGGAGTACCATGACTTCACTGTTGAAATGGCATGCGATGATGAGTATAGTACAGCGTTTACCTACAAC
AGAGTCGCAGGCGTGCTCGAGATTGATCGTACCTGCTGTGGCGTCACAAAGGATGTCGTCTGTGTCAGAAAAATTAAAAT
CGCAGATACAGACAGAAAGCTAAAGCTTCGTTTCATCATGGACAGACAGTCTATAGAGCTCTTTATCAATGACGGACAGC
AGGTCGCAACCACAGCTATCTGTACACCTCTTCAGGCAGACGGAATCAGCTTTAATTGTGACGGAAGTGCTGTTGTGGAT
ATTGAAAAATATGATATAATATGA

Upstream 100 bases:

>100_bases
GACAGCTATCAGAAATCCCTTGAGAGTACGTCAGAGGGCAAATAGGTTACTGTTCATAATTCTGTATTACCGGCAAAGCA
AACAGAATTGGAGGATTTGA

Downstream 100 bases:

>100_bases
TATAATTTTATAATATGAAAGCATATCAGTAGAGCATACAAATAATAACCGGACAAATTATTGGTCCGGTTATTTGTAGA
ATAATATACATAATATTTTG

Product: sucrose-6-phosphate hydrolase

Products: NA

Alternate protein names: Invertase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSVYNGKIHLFYQYYPYLREWGPMHWGHSTTGDMIKWEQ
LPCALAPDEEYDKKGCFSGSAIEADGKHVLVYTGVTRIKLPDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCS
RIDFRDPKIWKEDDTYYLIVGSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGTMWECPDFFGLEDKHVLICSPQS
MKADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTLDYGMDFYAPQTTLLPDGRRVMIAWMKSWDACVVPDSQDWQGMMT
LPRELEIKDGRIWQKPVKEIENYRANKCHYTDKKIDCYTTFDGIKGRTLDMTVELSGEEYHDFTVEMACDDEYSTAFTYN
RVAGVLEIDRTCCGVTKDVVCVRKIKIADTDRKLKLRFIMDRQSIELFINDGQQVATTAICTPLQADGISFNCDGSAVVD
IEKYDII

Sequences:

>Translated_487_residues
MSELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSVYNGKIHLFYQYYPYLREWGPMHWGHSTTGDMIKWEQ
LPCALAPDEEYDKKGCFSGSAIEADGKHVLVYTGVTRIKLPDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCS
RIDFRDPKIWKEDDTYYLIVGSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGTMWECPDFFGLEDKHVLICSPQS
MKADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTLDYGMDFYAPQTTLLPDGRRVMIAWMKSWDACVVPDSQDWQGMMT
LPRELEIKDGRIWQKPVKEIENYRANKCHYTDKKIDCYTTFDGIKGRTLDMTVELSGEEYHDFTVEMACDDEYSTAFTYN
RVAGVLEIDRTCCGVTKDVVCVRKIKIADTDRKLKLRFIMDRQSIELFINDGQQVATTAICTPLQADGISFNCDGSAVVD
IEKYDII
>Mature_486_residues
SELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSVYNGKIHLFYQYYPYLREWGPMHWGHSTTGDMIKWEQL
PCALAPDEEYDKKGCFSGSAIEADGKHVLVYTGVTRIKLPDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCSR
IDFRDPKIWKEDDTYYLIVGSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGTMWECPDFFGLEDKHVLICSPQSM
KADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTLDYGMDFYAPQTTLLPDGRRVMIAWMKSWDACVVPDSQDWQGMMTL
PRELEIKDGRIWQKPVKEIENYRANKCHYTDKKIDCYTTFDGIKGRTLDMTVELSGEEYHDFTVEMACDDEYSTAFTYNR
VAGVLEIDRTCCGVTKDVVCVRKIKIADTDRKLKLRFIMDRQSIELFINDGQQVATTAICTPLQADGISFNCDGSAVVDI
EKYDII

Specific function: May prevent the potential hasard of excessive sucrose accumulation [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=364, Percent_Identity=26.3736263736264, Blast_Score=126, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 55667; Mature: 55536

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00268 CECROPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSVYNGKIHLFYQYYPYLR
CHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCEEEEEEEECCHHH
EWGPMHWGHSTTGDMIKWEQLPCALAPDEEYDKKGCFSGSAIEADGKHVLVYTGVTRIKL
HCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCEEECCCCEEEEEECEEEEEC
PDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCSRIDFRDPKIWKEDDTYYLIV
CCCCCHHHCCCEEEEECCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGTMWECPDFFGLEDKHVLICSPQS
CCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECCCC
MKADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTLDYGMDFYAPQTTLLPDGRRVMIAW
CCCCCEEEECCCCEEEEEECCCCCCCEEEECCCCEEECCCEEECCCEEECCCCCEEEEEE
MKSWDACVVPDSQDWQGMMTLPRELEIKDGRIWQKPVKEIENYRANKCHYTDKKIDCYTT
ECCCCEEECCCCCCCCCEECCCCEEEECCCEEHHHHHHHHHHCCCCCEECCCCEEEEEEE
FDGIKGRTLDMTVELSGEEYHDFTVEMACDDEYSTAFTYNRVAGVLEIDRTCCGVTKDVV
CCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEEEHHHHEEEEEECHHHCCCCCCEE
CVRKIKIADTDRKLKLRFIMDRQSIELFINDGQQVATTAICTPLQADGISFNCDGSAVVD
EEEEEEEECCCCEEEEEEEECCCEEEEEEECCCEEEEHEEECCCCCCCEEEECCCCEEEE
IEKYDII
EEEECCC
>Mature Secondary Structure 
SELLEKARSYEAKKIAATDKESKPLFHISAPAGWINDPNGFSVYNGKIHLFYQYYPYLR
HHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCEEEEEEEECCHHH
EWGPMHWGHSTTGDMIKWEQLPCALAPDEEYDKKGCFSGSAIEADGKHVLVYTGVTRIKL
HCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCEEECCCCEEEEEECEEEEEC
PDGSEQERQNQCIAFGDGLNYVKHENNPVVTGDMLPENCSRIDFRDPKIWKEDDTYYLIV
CCCCCHHHCCCEEEEECCCCEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEE
GSKDLNNVGQVVLCSSKNLTDWQFETILAANSTGKIGTMWECPDFFGLEDKHVLICSPQS
CCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCCEEEEECCCC
MKADKYEFHNGHNSVYFLGDYDKENHVFIKEEPHTLDYGMDFYAPQTTLLPDGRRVMIAW
CCCCCEEEECCCCEEEEEECCCCCCCEEEECCCCEEECCCEEECCCEEECCCCCEEEEEE
MKSWDACVVPDSQDWQGMMTLPRELEIKDGRIWQKPVKEIENYRANKCHYTDKKIDCYTT
ECCCCEEECCCCCCCCCEECCCCEEEECCCEEHHHHHHHHHHCCCCCEECCCCEEEEEEE
FDGIKGRTLDMTVELSGEEYHDFTVEMACDDEYSTAFTYNRVAGVLEIDRTCCGVTKDVV
CCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCEEEEHHHHEEEEEECHHHCCCCCCEE
CVRKIKIADTDRKLKLRFIMDRQSIELFINDGQQVATTAICTPLQADGISFNCDGSAVVD
EEEEEEEECCCCEEEEEEEECCCEEEEEEECCCEEEEHEEECCCCCCCEEEECCCCEEEE
IEKYDII
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2556373 [H]