Click here to switch to the map view.

The map label for this gene is vsr [H]

Identifier: 238922587

GI number: 238922587

Start: 155933

End: 156400

Strand: Direct

Name: vsr [H]

Synonym: EUBREC_0161

Alternate gene names: 238922587

Gene position: 155933-156400 (Clockwise)

Preceding gene: 238922586

Following gene: 238922588

Centisome position: 4.52

GC content: 31.84

Gene sequence:

>468_bases
ATGATGGAGACACAGTATAAGAAGGTGATATATATGGCGGATGTTTTGACTAAAGAACAACGGCATAAAAACATGAAAAA
TATTCATGGAAAAGATACTAAAATTGAAATTATCCTTAGAAAAGCTTTATGGGCAAAAGGTTATCGGTACCGGAAGAATT
ATAAAAAGTTACCGGGAAATCCGGATATTGTGTTGACAAAATATAAGATAGCAATATTTTGCGATGGGGAATTTTTTCAT
GGAAAAGACTGGGAAGTGCTAAAGCCAAGGCTACAGAAAAGTAATAATAGTGCATTTTGGGTGAACAAAATTTCTAAAAA
TAGAGAAAGGGATGATATGGTTAATAAAAAATTGTTATTTATGGGATGGACGGTTATAAGATTTTGGGGTAAAGATATAA
AAAAGCATACAGATGAGTGTGTAAAGGCTATAGAAGAGATAATATTTGATATAAAGATGGAGGATTGA

Upstream 100 bases:

>100_bases
AATTTTAATGCAAAACTGAAAAGAGAAAGTTTTACTGTGGCAGAGCTTGAGGATATAGCAGAAGCCGCGGGAGTTACTTT
TGAAAGAAGTTTTGTGTTGG

Downstream 100 bases:

>100_bases
TTCTATGAAGAGTAGATTTGAAATAATGGGGGGAGGATATTAGGAAACAGACGCGTGAACTTGTAATGAGTTTTATGCAA
CAGAGTGAAGCGTGTCAACC

Product: very short patch repair endonuclease, putative

Products: DNA [C]

Alternate protein names: V.NmeDIP [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MMETQYKKVIYMADVLTKEQRHKNMKNIHGKDTKIEIILRKALWAKGYRYRKNYKKLPGNPDIVLTKYKIAIFCDGEFFH
GKDWEVLKPRLQKSNNSAFWVNKISKNRERDDMVNKKLLFMGWTVIRFWGKDIKKHTDECVKAIEEIIFDIKMED

Sequences:

>Translated_155_residues
MMETQYKKVIYMADVLTKEQRHKNMKNIHGKDTKIEIILRKALWAKGYRYRKNYKKLPGNPDIVLTKYKIAIFCDGEFFH
GKDWEVLKPRLQKSNNSAFWVNKISKNRERDDMVNKKLLFMGWTVIRFWGKDIKKHTDECVKAIEEIIFDIKMED
>Mature_155_residues
MMETQYKKVIYMADVLTKEQRHKNMKNIHGKDTKIEIILRKALWAKGYRYRKNYKKLPGNPDIVLTKYKIAIFCDGEFFH
GKDWEVLKPRLQKSNNSAFWVNKISKNRERDDMVNKKLLFMGWTVIRFWGKDIKKHTDECVKAIEEIIFDIKMED

Specific function: May nick NmeDIP sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=133, Percent_Identity=36.8421052631579, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 18676; Mature: 18676

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMETQYKKVIYMADVLTKEQRHKNMKNIHGKDTKIEIILRKALWAKGYRYRKNYKKLPGN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCC
PDIVLTKYKIAIFCDGEFFHGKDWEVLKPRLQKSNNSAFWVNKISKNRERDDMVNKKLLF
CCEEEEEEEEEEEECCCEECCCCHHHHHHHHHCCCCCEEEEECHHCCCCHHHHHHHHHHH
MGWTVIRFWGKDIKKHTDECVKAIEEIIFDIKMED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMETQYKKVIYMADVLTKEQRHKNMKNIHGKDTKIEIILRKALWAKGYRYRKNYKKLPGN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCC
PDIVLTKYKIAIFCDGEFFHGKDWEVLKPRLQKSNNSAFWVNKISKNRERDDMVNKKLLF
CCEEEEEEEEEEEECCCEECCCCHHHHHHHHHCCCCCEEEEECHHCCCCHHHHHHHHHHH
MGWTVIRFWGKDIKKHTDECVKAIEEIIFDIKMED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10671450 [H]