Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 238922484

GI number: 238922484

Start: 62971

End: 63951

Strand: Direct

Name: birA [H]

Synonym: EUBREC_0058

Alternate gene names: 238922484

Gene position: 62971-63951 (Clockwise)

Preceding gene: 238922483

Following gene: 238922485

Centisome position: 1.83

GC content: 49.85

Gene sequence:

>981_bases
ATGAAGGAAGAAATACTTAGACTTCTACGCAGCGCTGACGGCTATATTTCAGGGCAGGAGCTATGCAACCGGTTCAGAGT
ATCGCGCACAGCCGTGTGGAAGGCCATAAACCAGCTAAAGGAAGCCGGATATGAGATAGAGGCACAACAGAATAAGGGCT
ACAGGCTCATGGCAGCGCCGGATCTGATGACAGAGGCGGAGATAAAAAGCCTCATGCACACAGACTGGGTGGCAAAGGAA
GTGCTGTATTTTGATACAATAGACTCCACCAACATCAAGGCTCAGGAGCTTGCCGAAAAAGGCTATCCGAGCGGCACCTT
AGTCGTAGCGGACAAGCAGGAGTCAGGCAAGGGGCGCCGCGGCCGCAGCTGGGTATCGCCGTCCGGCACAGGTATTTTCA
TGACACTTATGATAAAGCCTGATATCAATCCAAACAATGCATCCATGCTCACGCTTGTGGCAGCTCTTGCTGTCGCAAAG
GCAATAACAAGCGTTACAGGCGAGGAAGCCCTGATAAAGTGGCCAAACGACATTGTGGTAAACGGCAAAAAGGTGTGCGG
CATACTCACAGAGATGAATGCGCAGTTTGACTACATCAACCATATTGTTGTAGGTATCGGTATCAACGTGCACAATGAGA
GCTTTCCGGAGGAAATCAGTCAGATGGCGAGCTCACTCATGATAGAAGCAGGTGGCAAAAGATTTCACCGTGCGCAGATA
ATCGCAGAGACCATGTCATACTTTGAGCAGTATTATGACACATTTTTAAAGACGCAGGATCTTTCAGCCCTCGTCAGGGA
GTATGATGAGCTGTTGGTCAACAGGAACAAATCAGTAAGGGTACTGGATCCGAAGGAGCCGTTTGACGGAAAAGCGATGG
GCATCACGCCTAAGGGCGAGCTGATCGTGGACACATGGGAGTCGCGCAAGCTGGTTTCAAGCGGAGAAGTATCAGTGAGA
GGAATATACGGATATGTCTAA

Upstream 100 bases:

>100_bases
GTATGCACTCAATCAAGGCTATCATGCGTGCAACACTTGCGGACTAGTACGGCAAGCAATGACCGCAACAAAAGATTGAC
GGAAAGCTAAGGAATAAAGT

Downstream 100 bases:

>100_bases
AGAGGTAGTACTTTGCGTGTCAAACGCATATCAGAAAAAATATTATCTGAACGAGAATTTTAACGGACTTCCACAGACTA
TAAGGGACGAGCTCAAAATC

Product: putative transcriptional repressor of the biotin operon birA

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKEEILRLLRSADGYISGQELCNRFRVSRTAVWKAINQLKEAGYEIEAQQNKGYRLMAAPDLMTEAEIKSLMHTDWVAKE
VLYFDTIDSTNIKAQELAEKGYPSGTLVVADKQESGKGRRGRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAK
AITSVTGEEALIKWPNDIVVNGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEEISQMASSLMIEAGGKRFHRAQI
IAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRNKSVRVLDPKEPFDGKAMGITPKGELIVDTWESRKLVSSGEVSVR
GIYGYV

Sequences:

>Translated_326_residues
MKEEILRLLRSADGYISGQELCNRFRVSRTAVWKAINQLKEAGYEIEAQQNKGYRLMAAPDLMTEAEIKSLMHTDWVAKE
VLYFDTIDSTNIKAQELAEKGYPSGTLVVADKQESGKGRRGRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAK
AITSVTGEEALIKWPNDIVVNGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEEISQMASSLMIEAGGKRFHRAQI
IAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRNKSVRVLDPKEPFDGKAMGITPKGELIVDTWESRKLVSSGEVSVR
GIYGYV
>Mature_326_residues
MKEEILRLLRSADGYISGQELCNRFRVSRTAVWKAINQLKEAGYEIEAQQNKGYRLMAAPDLMTEAEIKSLMHTDWVAKE
VLYFDTIDSTNIKAQELAEKGYPSGTLVVADKQESGKGRRGRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAK
AITSVTGEEALIKWPNDIVVNGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEEISQMASSLMIEAGGKRFHRAQI
IAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRNKSVRVLDPKEPFDGKAMGITPKGELIVDTWESRKLVSSGEVSVR
GIYGYV

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=308, Percent_Identity=26.6233766233766, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36373; Mature: 36373

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEILRLLRSADGYISGQELCNRFRVSRTAVWKAINQLKEAGYEIEAQQNKGYRLMAAP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECC
DLMTEAEIKSLMHTDWVAKEVLYFDTIDSTNIKAQELAEKGYPSGTLVVADKQESGKGRR
CHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCC
GRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAKAITSVTGEEALIKWPNDIVV
CCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEE
NGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEEISQMASSLMIEAGGKRFHRAQI
CCHHHHHHHHHCCCCHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
IAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRNKSVRVLDPKEPFDGKAMGITPKGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCCC
LIVDTWESRKLVSSGEVSVRGIYGYV
EEEEECCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKEEILRLLRSADGYISGQELCNRFRVSRTAVWKAINQLKEAGYEIEAQQNKGYRLMAAP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECC
DLMTEAEIKSLMHTDWVAKEVLYFDTIDSTNIKAQELAEKGYPSGTLVVADKQESGKGRR
CHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCC
GRSWVSPSGTGIFMTLMIKPDINPNNASMLTLVAALAVAKAITSVTGEEALIKWPNDIVV
CCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEE
NGKKVCGILTEMNAQFDYINHIVVGIGINVHNESFPEEISQMASSLMIEAGGKRFHRAQI
CCHHHHHHHHHCCCCHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
IAETMSYFEQYYDTFLKTQDLSALVREYDELLVNRNKSVRVLDPKEPFDGKAMGITPKGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCCC
LIVDTWESRKLVSSGEVSVRGIYGYV
EEEEECCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]