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The map label for this gene is dnaN [H]

Identifier: 238922434

GI number: 238922434

Start: 1648

End: 2760

Strand: Direct

Name: dnaN [H]

Synonym: EUBREC_0002

Alternate gene names: 238922434

Gene position: 1648-2760 (Clockwise)

Preceding gene: 238922433

Following gene: 238922435

Centisome position: 0.05

GC content: 37.74

Gene sequence:

>1113_bases
ATGAAACTTATTTGTTCAAAAGCAAATTTACTGAAAGGTGTTAATATCGTATCGAAGGCAGTACCTACACGTACTACAAT
GGCAATTCTCGAGTGCATACTTATTGATGCATCCGCAAACGAGATTAAGCTTATGGCTAACGATATGGAGCTTGGAATCG
AAACAATAATAGACGGAACTATCGAAGAGAGAGGAATTATCGCTCTTGATGCCAAGATTTTTTCAGAAATAGTGAGAAAG
CTTCCTGACAATGATGTAACAATTGAGACAGATGCTTCCTTCAAGACCGTCATATCATGTGAAAAGGCCAAGTTCAATAT
CATCGGAAAATCAGGTGATGATTTTTCTTACATACCATATGTTGAGAGAAATGAGTCTATTGTCCTTTCACAATTTACCC
TTAAAGAGGTTATCAGACAGACAATTTTCTCAATTGCCGACAATGACAACAATAAACTGATGACAGGTGAGCTTTTTGAG
ATTGAGGAGAATAAGCTTCGAGTTGTTTCTCTTGACGGACACAGAATATCTATCAGATATATCGAGATGAAAAATCACTA
TGACAGCAAGAAGGTTGTTGTTCCCGGCAAGACTCTGCAGGAAATAAGCAAGATTATTCCGGGTTCAGCGGATGAGGATG
TGGTTATCTATATCACTAACAATCATATTGTTTTTGAATTTGAAAATACTACTGTTGTTTCACGTCTTATCGAGGGTGAG
TATTTCAAGATTGACCAGATGCTTTCATCTGACTACGACACTAAGGTTCGCATCAATAAGCGTGAGCTTCTTGACTGTAT
AGATCGTGCTACTCTTCTTGTCAAGGAGGGAGATAAGAAGCCTATTATCATGAATATCACAGATGGCAATATGGAGCTTC
GCATCAATTCATTTATCGGTTCCATGAATGAGGACATCGATATCGACAAAGATGGAAAAGATATTATGATAGGCTTTAAT
CCTAAGTTCTTCATAGATGCACTTCGTGTTATCGATGAGGAGGAGGTTAATTTATACATGGTCAATCCAAAGGCACCATG
CTTCATTAAGGATGATGAGGGCAAGTTTATTTACCTCATTCTTCCGGTAAATTTTAATACAGCAGCAAACTAG

Upstream 100 bases:

>100_bases
AGGCTTTGAAGGGGTTTTGAACTTTTTAACAGCCCCTAAGAATAATATTACGAATATCTTTTAAATATTTATATATACGC
GCAGGAAGGAGTTTTTCTAA

Downstream 100 bases:

>100_bases
GAGATTTTATGAAACAGATTTCTATAAGAGAACAGGATGAGTTCATTAAGCTGGGACAGGCTCTTAAAAAGGCCGACCTT
GTGAGCTCGGGAGTTGAGGC

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGTIEERGIIALDAKIFSEIVRK
LPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPYVERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFE
IEENKLRVVSLDGHRISIRYIEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGE
YFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKEGDKKPIIMNITDGNMELRINSFIGSMNEDIDIDKDGKDIMIGFN
PKFFIDALRVIDEEEVNLYMVNPKAPCFIKDDEGKFIYLILPVNFNTAAN

Sequences:

>Translated_370_residues
MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGTIEERGIIALDAKIFSEIVRK
LPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPYVERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFE
IEENKLRVVSLDGHRISIRYIEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGE
YFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKEGDKKPIIMNITDGNMELRINSFIGSMNEDIDIDKDGKDIMIGFN
PKFFIDALRVIDEEEVNLYMVNPKAPCFIKDDEGKFIYLILPVNFNTAAN
>Mature_370_residues
MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGTIEERGIIALDAKIFSEIVRK
LPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPYVERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFE
IEENKLRVVSLDGHRISIRYIEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGE
YFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKEGDKKPIIMNITDGNMELRINSFIGSMNEDIDIDKDGKDIMIGFN
PKFFIDALRVIDEEEVNLYMVNPKAPCFIKDDEGKFIYLILPVNFNTAAN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=369, Percent_Identity=23.3062330623306, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41904; Mature: 41904

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGT
CEEEECCHHHHHCCHHHHHCCCCHHHHHHHEEEEEECCCCEEEEEECCHHHCHHHHCCCC
IEERGIIALDAKIFSEIVRKLPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPY
CCCCCEEEECHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEEECCCCCCEECCE
VERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFEIEENKLRVVSLDGHRISIRY
EECCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCEEEEEECCCEEEEEE
IEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGE
EEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHCCC
YFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKEGDKKPIIMNITDGNMELRINSFIG
EEEEHHHHCCCCCCEEEECHHHHHHHHHHEEEEEECCCCCCEEEEEECCCEEEEEEHHHC
SMNEDIDIDKDGKDIMIGFNPKFFIDALRVIDEEEVNLYMVNPKAPCFIKDDEGKFIYLI
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEE
LPVNFNTAAN
EEECCCCCCC
>Mature Secondary Structure
MKLICSKANLLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGT
CEEEECCHHHHHCCHHHHHCCCCHHHHHHHEEEEEECCCCEEEEEECCHHHCHHHHCCCC
IEERGIIALDAKIFSEIVRKLPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPY
CCCCCEEEECHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEEECCCCCCEECCE
VERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFEIEENKLRVVSLDGHRISIRY
EECCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCEEEEEECCCEEEEEE
IEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGE
EEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCHHHHHHHCCC
YFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKEGDKKPIIMNITDGNMELRINSFIG
EEEEHHHHCCCCCCEEEECHHHHHHHHHHEEEEEECCCCCCEEEEEECCCEEEEEEHHHC
SMNEDIDIDKDGKDIMIGFNPKFFIDALRVIDEEEVNLYMVNPKAPCFIKDDEGKFIYLI
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEE
LPVNFNTAAN
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA