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The map label for this gene is thrS [H]

Identifier: 238922273

GI number: 238922273

Start: 469217

End: 470992

Strand: Reverse

Name: thrS [H]

Synonym: EUBELI_20508

Alternate gene names: 238922273

Gene position: 470992-469217 (Counterclockwise)

Preceding gene: 238922274

Following gene: 238922272

Centisome position: 75.15

GC content: 39.19

Gene sequence:

>1776_bases
ATGGTTAATTTTAAGGAAGATGAAAGACTGAATACTCTTAATCATTCATGTGCACATGTTATGGCACAGGCAGTTAAGCA
TTTATATCCACATGCTAAGTTCTGGGTTGGACCAGTAGTTAAAGAGGGATTCTATTATGATATAGACCTTGGAGATAATG
TAATCAATGACGATGCTATTGAAGCTATCGAAAAAGAAATGAAGAAAATCTGCAAGGAAGGCAAGAAGATATACAGAAGA
GAAGTTTCTAAGGAAGAAGCCCTTGAGATGTTCAAGGATGATATGTATAAGCTTGATCTTATAAGCGGTCTTGAAGATGG
CAACATTACAGTATATGACCAGGGCGATTTCACAGACCTCTGTCGTGGACCTCATGTCGATAATACTAAGCTCTGCAAGA
ACTTCAAGCTTGTTAAATATTCTGGTGTATACTGGAAGGGCGATGCCAGCAATCATGTAATGCAGAGAATCTATGGTGTA
TGTTTCCCAACAGCTGAGGAGCTTGAAGAACATCTTAAGCTTCTTGAGGAAGCAAAGGAACGTGACCACAGAAAGATTGG
CAAGGAAATGGAACTTTTCATGTCAGATGACTTAATCGGACGTGGACTTCCAATGTTTTTACCAAAGGGATATACAGTAT
GGCAGGAGCTTGAGAATTACATTAAGGACAAGGAAAGAAAGCTTGGATACCTTCATGTAATGACACCATGTGTTGGTACA
GTTAATCTTTACAAGACATCAGGCCACTGGGATCACTACAAGGAAAACATGTTCCCAGCTATGGAAGTTGAAGGCGAATC
ATTTGTCTTAAGACCAATGAACTGCCCTCACCACATGATGATATATGCTAACAAAATGCATTCTTACAAGGATCTTCCTA
TAAGAATCGGTGAGATTGCACATGACTTCAGATTTGAGGCAAGTGGAACATTAAAGGGTATTGAGAGAGGAAGACATTTT
TGCCAGAATGATGCCCACCTTTTCGTAACACCAGAGCAGATTGAGTCAGAATTTGCCAAGGTTGTAGACCTTATATTTGA
GACGTATAAGGATTTCAATATTACAGATTACCGCTGTGTTCTCTCATTAAGAGATCCAGAGGATAAGGTAAAGTATCATG
ATGATGATGAAATGTGGAACAATGCTGAGAACGCATTAAGAAAGGTTCTTAATGATATCGGAATTGAGTACACAGAGGAA
ATCGGAGAGGCTGCATTCTACGGACCTAAGCTTGATGTAAATGTTAAGCCTGCTATCGGTAATGAATATACTTTATCAAC
ATGCCAGCTTGATTTCTGTCTTCCATCTAAGTTCAACTTAACATACATTGATAAAGATGGACAGAGAAAGACACCTGTAG
TTCTTCACAGAGCTATCCTTGGTTCTCTTGACAGATTCATGGCTTATATACTTGAGGAGACAAAGGGTAACCTTCCATTA
TGGCTTGCTCCTGTACAGGCTACAATTCTTCCGGTTAAGAATGAAGATGAAGAGTTAAATGCTTATGCTCATGGTCTCTA
TGATTATCTTGCAGATAATGGAATCAGAGTTGAGATTGATGAGAGAGCAGAAAAGCTTGGATACAGAGTAAGAGAAGCTC
AGGTAAAGAAGATTCCTTACCTTATTGTGCTTGGTAAGAATGAAGCAGCAGATGGAACTGTAAGCTACAGACTTCACGGA
GAGCAGAAGTCAACTACAGTATCTAAGGACGAATTTGTTGCAATGCTTAAGGATGAGATTGCTACTAAGAAGAATAATCC
TGCAGCAGCTAAATAA

Upstream 100 bases:

>100_bases
GCACCGCGACAGTGACAGAGCGAAGCTCTGGAACATGAGCTACGAGCACAAGCAGCGATGAGAATAGCAACAAAAATATT
AACACAAAAGGAGATTTATC

Downstream 100 bases:

>100_bases
TCAGACTGACCTTACAGAAAGAAGCGTGACAATATGTAATGTTTTATGTATTGTCATGCTTCTTGTTTATATTTCAGAAA
ACAATAGGAAAATAAATGGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MVNFKEDERLNTLNHSCAHVMAQAVKHLYPHAKFWVGPVVKEGFYYDIDLGDNVINDDAIEAIEKEMKKICKEGKKIYRR
EVSKEEALEMFKDDMYKLDLISGLEDGNITVYDQGDFTDLCRGPHVDNTKLCKNFKLVKYSGVYWKGDASNHVMQRIYGV
CFPTAEELEEHLKLLEEAKERDHRKIGKEMELFMSDDLIGRGLPMFLPKGYTVWQELENYIKDKERKLGYLHVMTPCVGT
VNLYKTSGHWDHYKENMFPAMEVEGESFVLRPMNCPHHMMIYANKMHSYKDLPIRIGEIAHDFRFEASGTLKGIERGRHF
CQNDAHLFVTPEQIESEFAKVVDLIFETYKDFNITDYRCVLSLRDPEDKVKYHDDDEMWNNAENALRKVLNDIGIEYTEE
IGEAAFYGPKLDVNVKPAIGNEYTLSTCQLDFCLPSKFNLTYIDKDGQRKTPVVLHRAILGSLDRFMAYILEETKGNLPL
WLAPVQATILPVKNEDEELNAYAHGLYDYLADNGIRVEIDERAEKLGYRVREAQVKKIPYLIVLGKNEAADGTVSYRLHG
EQKSTTVSKDEFVAMLKDEIATKKNNPAAAK

Sequences:

>Translated_591_residues
MVNFKEDERLNTLNHSCAHVMAQAVKHLYPHAKFWVGPVVKEGFYYDIDLGDNVINDDAIEAIEKEMKKICKEGKKIYRR
EVSKEEALEMFKDDMYKLDLISGLEDGNITVYDQGDFTDLCRGPHVDNTKLCKNFKLVKYSGVYWKGDASNHVMQRIYGV
CFPTAEELEEHLKLLEEAKERDHRKIGKEMELFMSDDLIGRGLPMFLPKGYTVWQELENYIKDKERKLGYLHVMTPCVGT
VNLYKTSGHWDHYKENMFPAMEVEGESFVLRPMNCPHHMMIYANKMHSYKDLPIRIGEIAHDFRFEASGTLKGIERGRHF
CQNDAHLFVTPEQIESEFAKVVDLIFETYKDFNITDYRCVLSLRDPEDKVKYHDDDEMWNNAENALRKVLNDIGIEYTEE
IGEAAFYGPKLDVNVKPAIGNEYTLSTCQLDFCLPSKFNLTYIDKDGQRKTPVVLHRAILGSLDRFMAYILEETKGNLPL
WLAPVQATILPVKNEDEELNAYAHGLYDYLADNGIRVEIDERAEKLGYRVREAQVKKIPYLIVLGKNEAADGTVSYRLHG
EQKSTTVSKDEFVAMLKDEIATKKNNPAAAK
>Mature_591_residues
MVNFKEDERLNTLNHSCAHVMAQAVKHLYPHAKFWVGPVVKEGFYYDIDLGDNVINDDAIEAIEKEMKKICKEGKKIYRR
EVSKEEALEMFKDDMYKLDLISGLEDGNITVYDQGDFTDLCRGPHVDNTKLCKNFKLVKYSGVYWKGDASNHVMQRIYGV
CFPTAEELEEHLKLLEEAKERDHRKIGKEMELFMSDDLIGRGLPMFLPKGYTVWQELENYIKDKERKLGYLHVMTPCVGT
VNLYKTSGHWDHYKENMFPAMEVEGESFVLRPMNCPHHMMIYANKMHSYKDLPIRIGEIAHDFRFEASGTLKGIERGRHF
CQNDAHLFVTPEQIESEFAKVVDLIFETYKDFNITDYRCVLSLRDPEDKVKYHDDDEMWNNAENALRKVLNDIGIEYTEE
IGEAAFYGPKLDVNVKPAIGNEYTLSTCQLDFCLPSKFNLTYIDKDGQRKTPVVLHRAILGSLDRFMAYILEETKGNLPL
WLAPVQATILPVKNEDEELNAYAHGLYDYLADNGIRVEIDERAEKLGYRVREAQVKKIPYLIVLGKNEAADGTVSYRLHG
EQKSTTVSKDEFVAMLKDEIATKKNNPAAAK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=569, Percent_Identity=38.6643233743409, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI38202255, Length=578, Percent_Identity=39.1003460207612, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI20070344, Length=577, Percent_Identity=33.7954939341421, Blast_Score=335, Evalue=1e-91,
Organism=Escherichia coli, GI1788013, Length=588, Percent_Identity=37.2448979591837, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786392, Length=135, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=579, Percent_Identity=36.0967184801382, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71984192, Length=579, Percent_Identity=36.0967184801382, Blast_Score=389, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6322112, Length=598, Percent_Identity=36.1204013377926, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322654, Length=405, Percent_Identity=33.8271604938272, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24583839, Length=579, Percent_Identity=38.6873920552677, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24583842, Length=579, Percent_Identity=38.6873920552677, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI28574141, Length=579, Percent_Identity=38.6873920552677, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 68161; Mature: 68161

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFKEDERLNTLNHSCAHVMAQAVKHLYPHAKFWVGPVVKEGFYYDIDLGDNVINDDAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHCCEEEEEECCCCCCCHHHH
EAIEKEMKKICKEGKKIYRREVSKEEALEMFKDDMYKLDLISGLEDGNITVYDQGDFTDL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHHCCCCCCCEEEEECCCHHHH
CRGPHVDNTKLCKNFKLVKYSGVYWKGDASNHVMQRIYGVCFPTAEELEEHLKLLEEAKE
HCCCCCCCHHHHCCEEEEEEECEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDHRKIGKEMELFMSDDLIGRGLPMFLPKGYTVWQELENYIKDKERKLGYLHVMTPCVGT
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
VNLYKTSGHWDHYKENMFPAMEVEGESFVLRPMNCPHHMMIYANKMHSYKDLPIRIGEIA
EEEEEECCCCHHHHHCCCCCEEECCCEEEEECCCCCCEEEEEECHHCCCCCCCEEHHHHH
HDFRFEASGTLKGIERGRHFCQNDAHLFVTPEQIESEFAKVVDLIFETYKDFNITDYRCV
HHHEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LSLRDPEDKVKYHDDDEMWNNAENALRKVLNDIGIEYTEEIGEAAFYGPKLDVNVKPAIG
EEECCCHHCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHCCHHCCCCEECEEECCCCC
NEYTLSTCQLDFCLPSKFNLTYIDKDGQRKTPVVLHRAILGSLDRFMAYILEETKGNLPL
CCEEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
WLAPVQATILPVKNEDEELNAYAHGLYDYLADNGIRVEIDERAEKLGYRVREAQVKKIPY
EEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHHHHHCCCCE
LIVLGKNEAADGTVSYRLHGEQKSTTVSKDEFVAMLKDEIATKKNNPAAAK
EEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVNFKEDERLNTLNHSCAHVMAQAVKHLYPHAKFWVGPVVKEGFYYDIDLGDNVINDDAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHCCEEEEEECCCCCCCHHHH
EAIEKEMKKICKEGKKIYRREVSKEEALEMFKDDMYKLDLISGLEDGNITVYDQGDFTDL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEHHHCCCCCCCEEEEECCCHHHH
CRGPHVDNTKLCKNFKLVKYSGVYWKGDASNHVMQRIYGVCFPTAEELEEHLKLLEEAKE
HCCCCCCCHHHHCCEEEEEEECEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RDHRKIGKEMELFMSDDLIGRGLPMFLPKGYTVWQELENYIKDKERKLGYLHVMTPCVGT
HHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
VNLYKTSGHWDHYKENMFPAMEVEGESFVLRPMNCPHHMMIYANKMHSYKDLPIRIGEIA
EEEEEECCCCHHHHHCCCCCEEECCCEEEEECCCCCCEEEEEECHHCCCCCCCEEHHHHH
HDFRFEASGTLKGIERGRHFCQNDAHLFVTPEQIESEFAKVVDLIFETYKDFNITDYRCV
HHHEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
LSLRDPEDKVKYHDDDEMWNNAENALRKVLNDIGIEYTEEIGEAAFYGPKLDVNVKPAIG
EEECCCHHCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHCCHHCCCCEECEEECCCCC
NEYTLSTCQLDFCLPSKFNLTYIDKDGQRKTPVVLHRAILGSLDRFMAYILEETKGNLPL
CCEEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
WLAPVQATILPVKNEDEELNAYAHGLYDYLADNGIRVEIDERAEKLGYRVREAQVKKIPY
EEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHCCHHHHHHHCCCCE
LIVLGKNEAADGTVSYRLHGEQKSTTVSKDEFVAMLKDEIATKKNNPAAAK
EEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA