Click here to switch to the map view.

The map label for this gene is icaA [H]

Identifier: 238922212

GI number: 238922212

Start: 411747

End: 413144

Strand: Reverse

Name: icaA [H]

Synonym: EUBELI_20447

Alternate gene names: 238922212

Gene position: 413144-411747 (Counterclockwise)

Preceding gene: 238922213

Following gene: 238922211

Centisome position: 65.92

GC content: 34.84

Gene sequence:

>1398_bases
ATGGTAAAAATAATTCTTAATTCATTTCTTTTCTGGGGCGGATGGATAATTATTCCTTTCATTATGGAAATTGTTCCTGC
GATAGGAAGCGTTTTTCTTCTTATAAGAAGGAATCTGAGACATAAAATCCATAAAAAGGAACTTGAGGTTTATCCAGAGA
TTACGCTTATAATACCAGTATATAATTCGGCAGATACACTATATGGATGTATCAGATCGATTAATGACAGTACTTACCCG
AATGACAGGATAAGAATTTTTCTGGTTAATAATAAAGGAAAAGATAACAGTTTTGATATATATGCACAGTGTCAGCAGGA
ATTTCCAGAACTTCTGATGCAGTGGATGAATTCTGAACAGGGAAAGTCAAGAGCACTTAATCTGGCACTTTATAACAGTG
ATGGAAAGTACATTATCAATCTGGATAGTGATGGAATGCTTGAGAAGAATGCACTTGTCAATATGATTACAAGATTTGAG
AATGATACGGCTATTAACTGTATGACAGGATCTATTCTTACAGTGCCTGAGCAGATTAAGAAATATAAAGCAGGACCATC
AAGACTGCTCCGTGAACTTGAGTTTATGGAATATGCACAGGCTTTCCTTGCGGGAAGAAGTTATGCATCAGAGCTTAACT
CCGTGTATACATTGTCAGGTGCATTTTCAGCTTTCAGAAAATCAGCAGTTCTGAAGTCATGGATGTATAACACAGATACT
ATCTGTGAGGACACACATATTACATTTCAGATGAGGTATCTTCAGAAAGAACGGGTTGAAGTGTGTGAGGATGCACTGTT
TTTTGTTGATCCGATTGAAAATGTTAATAAACTATATACACAGAGACAGAGATGGCAGAGAGGAAGCCTTGAAGTATCCA
AGATGTTTATGGACAAGTCTTTCAAGGTAAAGAACCTTTTTACTAATATAAGTGTCAAAACTCTTTTGTATGACCATACA
TTTGCATTTCCAAGACTTATATGGTATCTGGCACTTATATGCCTGATTGTTGTGGGTTATTCTGGGAAAACGGTTATTCT
ATCTACAGCAATAATATTTGGTTTATATACGCTTATCGGATATCTGTATTTTATTGTTGTAGCATATTTCCTAAGAATTA
ATAAGGATATAAGAAGATATTACACGAGGCACTGGTGGTGCATACTGTTTCTGCCATTTTTTAATTTTGCTGTATTCTTT
ATAAGACTTGCAGGAATTATTAACAGTATTGAAACTGACAGCACATGGAAAACAATGAATCTGTCAGAGGAATGCAGGGC
ATTTGCAGATGTGGTTAAAAGTGATTTCAGTAAGCCTGTTAATGCAATAAAGAAAGTAAGAAATGCACTTAATTATGATG
TTGTAGAAGATAATAATAACAATAATCAGAGGGAATAA

Upstream 100 bases:

>100_bases
TTTATAGGAAGAATATTCTTCTATGTACTCAGTGTGTATCTGTATTTTTATGTATTTACGAAACCACAGATTGTTAAGAT
GAAAGTAGGAAACTTCTCAT

Downstream 100 bases:

>100_bases
CATGGAAAAGAAGCTTAATAAGAAGCGTGTTAAGAAGAATAAAAAGCCGAAAAATGTGCTTGTTATCACAACTATTTATG
TAATATGTTTCTCGGTTATT

Product: hypothetical protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVKIILNSFLFWGGWIIIPFIMEIVPAIGSVFLLIRRNLRHKIHKKELEVYPEITLIIPVYNSADTLYGCIRSINDSTYP
NDRIRIFLVNNKGKDNSFDIYAQCQQEFPELLMQWMNSEQGKSRALNLALYNSDGKYIINLDSDGMLEKNALVNMITRFE
NDTAINCMTGSILTVPEQIKKYKAGPSRLLRELEFMEYAQAFLAGRSYASELNSVYTLSGAFSAFRKSAVLKSWMYNTDT
ICEDTHITFQMRYLQKERVEVCEDALFFVDPIENVNKLYTQRQRWQRGSLEVSKMFMDKSFKVKNLFTNISVKTLLYDHT
FAFPRLIWYLALICLIVVGYSGKTVILSTAIIFGLYTLIGYLYFIVVAYFLRINKDIRRYYTRHWWCILFLPFFNFAVFF
IRLAGIINSIETDSTWKTMNLSEECRAFADVVKSDFSKPVNAIKKVRNALNYDVVEDNNNNNQRE

Sequences:

>Translated_465_residues
MVKIILNSFLFWGGWIIIPFIMEIVPAIGSVFLLIRRNLRHKIHKKELEVYPEITLIIPVYNSADTLYGCIRSINDSTYP
NDRIRIFLVNNKGKDNSFDIYAQCQQEFPELLMQWMNSEQGKSRALNLALYNSDGKYIINLDSDGMLEKNALVNMITRFE
NDTAINCMTGSILTVPEQIKKYKAGPSRLLRELEFMEYAQAFLAGRSYASELNSVYTLSGAFSAFRKSAVLKSWMYNTDT
ICEDTHITFQMRYLQKERVEVCEDALFFVDPIENVNKLYTQRQRWQRGSLEVSKMFMDKSFKVKNLFTNISVKTLLYDHT
FAFPRLIWYLALICLIVVGYSGKTVILSTAIIFGLYTLIGYLYFIVVAYFLRINKDIRRYYTRHWWCILFLPFFNFAVFF
IRLAGIINSIETDSTWKTMNLSEECRAFADVVKSDFSKPVNAIKKVRNALNYDVVEDNNNNNQRE
>Mature_465_residues
MVKIILNSFLFWGGWIIIPFIMEIVPAIGSVFLLIRRNLRHKIHKKELEVYPEITLIIPVYNSADTLYGCIRSINDSTYP
NDRIRIFLVNNKGKDNSFDIYAQCQQEFPELLMQWMNSEQGKSRALNLALYNSDGKYIINLDSDGMLEKNALVNMITRFE
NDTAINCMTGSILTVPEQIKKYKAGPSRLLRELEFMEYAQAFLAGRSYASELNSVYTLSGAFSAFRKSAVLKSWMYNTDT
ICEDTHITFQMRYLQKERVEVCEDALFFVDPIENVNKLYTQRQRWQRGSLEVSKMFMDKSFKVKNLFTNISVKTLLYDHT
FAFPRLIWYLALICLIVVGYSGKTVILSTAIIFGLYTLIGYLYFIVVAYFLRINKDIRRYYTRHWWCILFLPFFNFAVFF
IRLAGIINSIETDSTWKTMNLSEECRAFADVVKSDFSKPVNAIKKVRNALNYDVVEDNNNNNQRE

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=307, Percent_Identity=23.7785016286645, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 54267; Mature: 54267

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIILNSFLFWGGWIIIPFIMEIVPAIGSVFLLIRRNLRHKIHKKELEVYPEITLIIPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
YNSADTLYGCIRSINDSTYPNDRIRIFLVNNKGKDNSFDIYAQCQQEFPELLMQWMNSEQ
CCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GKSRALNLALYNSDGKYIINLDSDGMLEKNALVNMITRFENDTAINCMTGSILTVPEQIK
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCHHHHH
KYKAGPSRLLRELEFMEYAQAFLAGRSYASELNSVYTLSGAFSAFRKSAVLKSWMYNTDT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCC
ICEDTHITFQMRYLQKERVEVCEDALFFVDPIENVNKLYTQRQRWQRGSLEVSKMFMDKS
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
FKVKNLFTNISVKTLLYDHTFAFPRLIWYLALICLIVVGYSGKTVILSTAIIFGLYTLIG
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YLYFIVVAYFLRINKDIRRYYTRHWWCILFLPFFNFAVFFIRLAGIINSIETDSTWKTMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
LSEECRAFADVVKSDFSKPVNAIKKVRNALNYDVVEDNNNNNQRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MVKIILNSFLFWGGWIIIPFIMEIVPAIGSVFLLIRRNLRHKIHKKELEVYPEITLIIPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
YNSADTLYGCIRSINDSTYPNDRIRIFLVNNKGKDNSFDIYAQCQQEFPELLMQWMNSEQ
CCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GKSRALNLALYNSDGKYIINLDSDGMLEKNALVNMITRFENDTAINCMTGSILTVPEQIK
CCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCEECCHHHHH
KYKAGPSRLLRELEFMEYAQAFLAGRSYASELNSVYTLSGAFSAFRKSAVLKSWMYNTDT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCC
ICEDTHITFQMRYLQKERVEVCEDALFFVDPIENVNKLYTQRQRWQRGSLEVSKMFMDKS
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
FKVKNLFTNISVKTLLYDHTFAFPRLIWYLALICLIVVGYSGKTVILSTAIIFGLYTLIG
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YLYFIVVAYFLRINKDIRRYYTRHWWCILFLPFFNFAVFFIRLAGIINSIETDSTWKTMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
LSEECRAFADVVKSDFSKPVNAIKKVRNALNYDVVEDNNNNNQRE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]