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The map label for this gene is phnE [H]

Identifier: 238922182

GI number: 238922182

Start: 378852

End: 380393

Strand: Reverse

Name: phnE [H]

Synonym: EUBELI_20417

Alternate gene names: 238922182

Gene position: 380393-378852 (Counterclockwise)

Preceding gene: 238922183

Following gene: 238922180

Centisome position: 60.69

GC content: 40.14

Gene sequence:

>1542_bases
ATGAAAAGCATGAATAGAATGAAGGTTATCAGAAGAATTACGGCACTGGTAATGCTTTTTATAATAATATTCTGTTTTGC
TGTAACAGGCTTTGATATGAATGTAATTATAAAGAGAGGCAGTCAGGCTTTATTATTTATAAAAAGAATGTTCCCACCAG
ATACAGGATATTGTGGTAAGATTATAGAACCACTTATTAAGACTGTGCAGATGTCGATTGCGGGTACATTTATTGGTGCT
GTGTTTGGACTTTTTACAGCATTTTTATATGCAGGTAATCTTATTAAGAAGCCTTATGTAAGGATTGTAGTGAGAGTTAT
TGTACAGTGCGTAAGAACAATTCCTGTGCTTATACTCGCACTTGTAGCAACATTTATATGGGGACTTGGTGCGGCAGCGG
GTACGATTGCAATAGCTGTATCGACATGGGCTGTAATGGCAAGAATTGGTGGTGAGGATATTGAAAGCCTGACACTGAAG
CCATATGAAGCAGTTACAGCTATAGGAGCAGGAAAATTTAAAGCATTTTCACGGACAGTTATTATTGAACTGCTGCCGGG
ATATCTTAGCAATTCGCTTTATGTACTGGAAGCTAATATAAGACATGCGGCAATACTTGGCTATGTAGGTGCAGGTGGAA
TAGGACTACTGCTTAATGAAAAGATAAGCTGGCGTGAATACAGCAGGGTTGGAATGATTCTTGTTATGCTTTTTGTCGCA
GTGCTTGTGATTGAGGGAATTAGCACATTCCTGAAAGCATATCTTGATGGACGTATTAAGAGAAATACAACTGCAAATGT
GATTATAAGTGTATGTATCATGGTATTTTTTGTATACAGTCTCAGTGCAGTTAAAGATGTATCAACATCAAAGCTTGGTA
TTAAGGTTGTTGGTGCGATTATGCACGGAATTACACATCCGGACTGGGAATACATGTTTATGACCGGAAAGAGTGGCGTT
ATGTATCTTATGCTTGAAACTATTGCAATCGCAGTTGCGGGAACATGTGCCGGAGCAGTGTTTGCAGCAATACTTACTCT
TATTAATTCTAGAAAATTCGTTCCGGCACCGGTGGCTTTTATTGGCAGGCTTGTTGTTATGGCAATAAGAACTGTACCTG
TGTATGTGTATGGACTTATATTCATAAGGGTTACGGGACCTGGCAGCTTTGCCGGGGTGCTTACAATGATGATGTGCAGT
ATTGGACTTCTGACAAAGAGATTTACAGTTGCGGTTGACAACTGGAACATGGCGGCGTGGAATGCGTGCAAATGTGCAGG
AACAGGATTCATAGCAAGACTACGGTATGTGGCAGTACCTGAGCTTAAGATTCAGTTTAAGGATGCTGTGATGTACAGGT
TGGATGTGAATGTGCGATCGGCAAGTACATTAGGACTTGTTGGAGCAGGCGGAATCGGCGCCCCACTGATTGTCTACATG
AACAATTACAGATGGGACGCCGTTGGCTCAATTCTTATTGTATTATTTGTTGTAGTTCTTCTTATAGAACTTCTTTCAAA
ATGTTTAAAGAGAATCCACTAA

Upstream 100 bases:

>100_bases
TCAGACAGAATTATAGGAATTGCTGATGGCAGTCTTGTACTTGATAAAAAGTCGTCAGAGGTAACAGATGAGAATATTGA
ATTCATATATGGTGGTGTGT

Downstream 100 bases:

>100_bases
GCTTTCTTTAACCGGCTGTAGTCAATAACATATATGAAAATGTAAAGAACGATATAAAGAATTATTGCTCCGATTACATC
GACTACAGAATGCTGCTTTA

Product: phosphonate transport system permease protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKSMNRMKVIRRITALVMLFIIIFCFAVTGFDMNVIIKRGSQALLFIKRMFPPDTGYCGKIIEPLIKTVQMSIAGTFIGA
VFGLFTAFLYAGNLIKKPYVRIVVRVIVQCVRTIPVLILALVATFIWGLGAAAGTIAIAVSTWAVMARIGGEDIESLTLK
PYEAVTAIGAGKFKAFSRTVIIELLPGYLSNSLYVLEANIRHAAILGYVGAGGIGLLLNEKISWREYSRVGMILVMLFVA
VLVIEGISTFLKAYLDGRIKRNTTANVIISVCIMVFFVYSLSAVKDVSTSKLGIKVVGAIMHGITHPDWEYMFMTGKSGV
MYLMLETIAIAVAGTCAGAVFAAILTLINSRKFVPAPVAFIGRLVVMAIRTVPVYVYGLIFIRVTGPGSFAGVLTMMMCS
IGLLTKRFTVAVDNWNMAAWNACKCAGTGFIARLRYVAVPELKIQFKDAVMYRLDVNVRSASTLGLVGAGGIGAPLIVYM
NNYRWDAVGSILIVLFVVVLLIELLSKCLKRIH

Sequences:

>Translated_513_residues
MKSMNRMKVIRRITALVMLFIIIFCFAVTGFDMNVIIKRGSQALLFIKRMFPPDTGYCGKIIEPLIKTVQMSIAGTFIGA
VFGLFTAFLYAGNLIKKPYVRIVVRVIVQCVRTIPVLILALVATFIWGLGAAAGTIAIAVSTWAVMARIGGEDIESLTLK
PYEAVTAIGAGKFKAFSRTVIIELLPGYLSNSLYVLEANIRHAAILGYVGAGGIGLLLNEKISWREYSRVGMILVMLFVA
VLVIEGISTFLKAYLDGRIKRNTTANVIISVCIMVFFVYSLSAVKDVSTSKLGIKVVGAIMHGITHPDWEYMFMTGKSGV
MYLMLETIAIAVAGTCAGAVFAAILTLINSRKFVPAPVAFIGRLVVMAIRTVPVYVYGLIFIRVTGPGSFAGVLTMMMCS
IGLLTKRFTVAVDNWNMAAWNACKCAGTGFIARLRYVAVPELKIQFKDAVMYRLDVNVRSASTLGLVGAGGIGAPLIVYM
NNYRWDAVGSILIVLFVVVLLIELLSKCLKRIH
>Mature_513_residues
MKSMNRMKVIRRITALVMLFIIIFCFAVTGFDMNVIIKRGSQALLFIKRMFPPDTGYCGKIIEPLIKTVQMSIAGTFIGA
VFGLFTAFLYAGNLIKKPYVRIVVRVIVQCVRTIPVLILALVATFIWGLGAAAGTIAIAVSTWAVMARIGGEDIESLTLK
PYEAVTAIGAGKFKAFSRTVIIELLPGYLSNSLYVLEANIRHAAILGYVGAGGIGLLLNEKISWREYSRVGMILVMLFVA
VLVIEGISTFLKAYLDGRIKRNTTANVIISVCIMVFFVYSLSAVKDVSTSKLGIKVVGAIMHGITHPDWEYMFMTGKSGV
MYLMLETIAIAVAGTCAGAVFAAILTLINSRKFVPAPVAFIGRLVVMAIRTVPVYVYGLIFIRVTGPGSFAGVLTMMMCS
IGLLTKRFTVAVDNWNMAAWNACKCAGTGFIARLRYVAVPELKIQFKDAVMYRLDVNVRSASTLGLVGAGGIGAPLIVYM
NNYRWDAVGSILIVLFVVVLLIELLSKCLKRIH

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 55875; Mature: 55875

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMNRMKVIRRITALVMLFIIIFCFAVTGFDMNVIIKRGSQALLFIKRMFPPDTGYCGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHH
IIEPLIKTVQMSIAGTFIGAVFGLFTAFLYAGNLIKKPYVRIVVRVIVQCVRTIPVLILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LVATFIWGLGAAAGTIAIAVSTWAVMARIGGEDIESLTLKPYEAVTAIGAGKFKAFSRTV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHH
IIELLPGYLSNSLYVLEANIRHAAILGYVGAGGIGLLLNEKISWREYSRVGMILVMLFVA
HHHHHHHHHCCCEEEEEECCCHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VLVIEGISTFLKAYLDGRIKRNTTANVIISVCIMVFFVYSLSAVKDVSTSKLGIKVVGAI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MHGITHPDWEYMFMTGKSGVMYLMLETIAIAVAGTCAGAVFAAILTLINSRKFVPAPVAF
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IGRLVVMAIRTVPVYVYGLIFIRVTGPGSFAGVLTMMMCSIGLLTKRFTVAVDNWNMAAW
HHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
NACKCAGTGFIARLRYVAVPELKIQFKDAVMYRLDVNVRSASTLGLVGAGGIGAPLIVYM
CCHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCEECCCCCCEEECCCCCCCEEEEE
NNYRWDAVGSILIVLFVVVLLIELLSKCLKRIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSMNRMKVIRRITALVMLFIIIFCFAVTGFDMNVIIKRGSQALLFIKRMFPPDTGYCGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCHHH
IIEPLIKTVQMSIAGTFIGAVFGLFTAFLYAGNLIKKPYVRIVVRVIVQCVRTIPVLILA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHH
LVATFIWGLGAAAGTIAIAVSTWAVMARIGGEDIESLTLKPYEAVTAIGAGKFKAFSRTV
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHCCCHHHHHHHHH
IIELLPGYLSNSLYVLEANIRHAAILGYVGAGGIGLLLNEKISWREYSRVGMILVMLFVA
HHHHHHHHHCCCEEEEEECCCHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
VLVIEGISTFLKAYLDGRIKRNTTANVIISVCIMVFFVYSLSAVKDVSTSKLGIKVVGAI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MHGITHPDWEYMFMTGKSGVMYLMLETIAIAVAGTCAGAVFAAILTLINSRKFVPAPVAF
HHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IGRLVVMAIRTVPVYVYGLIFIRVTGPGSFAGVLTMMMCSIGLLTKRFTVAVDNWNMAAW
HHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
NACKCAGTGFIARLRYVAVPELKIQFKDAVMYRLDVNVRSASTLGLVGAGGIGAPLIVYM
CCHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCEECCCCCCEEECCCCCCCEEEEE
NNYRWDAVGSILIVLFVVVLLIELLSKCLKRIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA