Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 238922176

GI number: 238922176

Start: 372146

End: 373219

Strand: Reverse

Name: carA [H]

Synonym: EUBELI_20411

Alternate gene names: 238922176

Gene position: 373219-372146 (Counterclockwise)

Preceding gene: 238922177

Following gene: 238922175

Centisome position: 59.55

GC content: 39.11

Gene sequence:

>1074_bases
ATGAAAGCATTTTTGATACTTGAAGATGGAACGGTCTTTGAAGGAACCAGCATAGGTTCGACCCGCGAAGTTATTAGTGA
GATTGTCTTTAACACATCTATGACAGGATATCTGGAGGTGCTGACTGATCCTTCATACGCAGGACAGGCAGTAACAATGA
CATATCCTCTTATCGGTAATTACGGAGTGTGCTATAAGGATATGGAATCCCAGAAAGCATGGCCAGATGGATATATTGTA
CGTGAGCTTTCCAGAATGGCAAGCAACTTCAGAAATGAAGATCCTATCCAGAAATTCCTTCTTGATAATGATATCCCAGG
TATTTGTGGGATTGACACAAGAGCCCTTACTAAGATACTAAGAGAAAAGGGTACTATGAACGGTATGATTACAACTAATG
AGAATTACAATCTTGATGAGGTACTTCCTAAGATTAAGGAGTACAGTGTTAAGGGTGTTGTAAGAAAGGTTACATGTTCA
GAAAAGTATGTCCTTACAGGAAAAGGACCTAAGGTTGCTCTTATGGACTTTGGAGCAAAGAAGAATATTGCAAGATCACT
TAATAACAGAGGCTGTGAAGTTACAGTATATCCTGCATATACTAAGGCAGAAGAGATTCTTGCATCTAATCCTGATGGAA
TTATGTTATCTAACGGTCCTGGAGATCCTAAGGAATGTGTAGAGATAATTGAAGAAATTAAGAAGCTGTATAACAGCAAT
GTACCAATATTTGCAATCTGTCTTGGACATCAGCTTATGGCACTTGCTACAGGAGCTGATACTCACAGACTTAAGTACGG
ACACAGAGGTGCTAACCACCCTGTTAAGGATTTAAAGACTGGCAAATGTTATATATCATCACAGAATCATGGTTATGTTG
TAGATGAAAGCACATTAGACAGTAAGGTTGCAGTACCTGCATTTGAGAATGTTAATGATAAGACTAATGAGGGACTTGAA
TATGTTGGTAAGAATATATTCACAGTACAGTTCCATCCAGAAGCCTGCGCAGGTCCACAGGATACAGCGTACCTGTTTGA
CAGATTTATGGATATGATGAAGAAGGAGGACTAA

Upstream 100 bases:

>100_bases
AAAAAGGCATTTTTGTGTCTTTTTTTTTGCGCAAAAGTCATTTTAGCGTCTCTTGGCATATGATTAAATTAAATAAACGA
AAGGAATTAAGGTGGCATTC

Downstream 100 bases:

>100_bases
GAACATGCCAAAGATTGAGAGTATTAAGAAAGTATTAGTTATAGGTTCTGGTCCAATCGTTATTGGTCAGGCAGCAGAAT
TTGACTATGCCGGAACACAG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKAFLILEDGTVFEGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVTMTYPLIGNYGVCYKDMESQKAWPDGYIV
RELSRMASNFRNEDPIQKFLLDNDIPGICGIDTRALTKILREKGTMNGMITTNENYNLDEVLPKIKEYSVKGVVRKVTCS
EKYVLTGKGPKVALMDFGAKKNIARSLNNRGCEVTVYPAYTKAEEILASNPDGIMLSNGPGDPKECVEIIEEIKKLYNSN
VPIFAICLGHQLMALATGADTHRLKYGHRGANHPVKDLKTGKCYISSQNHGYVVDESTLDSKVAVPAFENVNDKTNEGLE
YVGKNIFTVQFHPEACAGPQDTAYLFDRFMDMMKKED

Sequences:

>Translated_357_residues
MKAFLILEDGTVFEGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVTMTYPLIGNYGVCYKDMESQKAWPDGYIV
RELSRMASNFRNEDPIQKFLLDNDIPGICGIDTRALTKILREKGTMNGMITTNENYNLDEVLPKIKEYSVKGVVRKVTCS
EKYVLTGKGPKVALMDFGAKKNIARSLNNRGCEVTVYPAYTKAEEILASNPDGIMLSNGPGDPKECVEIIEEIKKLYNSN
VPIFAICLGHQLMALATGADTHRLKYGHRGANHPVKDLKTGKCYISSQNHGYVVDESTLDSKVAVPAFENVNDKTNEGLE
YVGKNIFTVQFHPEACAGPQDTAYLFDRFMDMMKKED
>Mature_357_residues
MKAFLILEDGTVFEGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVTMTYPLIGNYGVCYKDMESQKAWPDGYIV
RELSRMASNFRNEDPIQKFLLDNDIPGICGIDTRALTKILREKGTMNGMITTNENYNLDEVLPKIKEYSVKGVVRKVTCS
EKYVLTGKGPKVALMDFGAKKNIARSLNNRGCEVTVYPAYTKAEEILASNPDGIMLSNGPGDPKECVEIIEEIKKLYNSN
VPIFAICLGHQLMALATGADTHRLKYGHRGANHPVKDLKTGKCYISSQNHGYVVDESTLDSKVAVPAFENVNDKTNEGLE
YVGKNIFTVQFHPEACAGPQDTAYLFDRFMDMMKKED

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=38.6486486486486, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI21361331, Length=372, Percent_Identity=38.7096774193548, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI169790915, Length=372, Percent_Identity=38.7096774193548, Blast_Score=233, Evalue=3e-61,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=45.8445040214477, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI193204318, Length=370, Percent_Identity=41.6216216216216, Blast_Score=276, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6324878, Length=372, Percent_Identity=43.5483870967742, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6322331, Length=393, Percent_Identity=36.6412213740458, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI45555749, Length=378, Percent_Identity=41.5343915343915, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=41.5343915343915, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39631; Mature: 39631

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLILEDGTVFEGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVTMTYPLIGN
CCEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCC
YGVCYKDMESQKAWPDGYIVRELSRMASNFRNEDPIQKFLLDNDIPGICGIDTRALTKIL
CCCEECCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
REKGTMNGMITTNENYNLDEVLPKIKEYSVKGVVRKVTCSEKYVLTGKGPKVALMDFGAK
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEEECCCH
KNIARSLNNRGCEVTVYPAYTKAEEILASNPDGIMLSNGPGDPKECVEIIEEIKKLYNSN
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
VPIFAICLGHQLMALATGADTHRLKYGHRGANHPVKDLKTGKCYISSQNHGYVVDESTLD
CCEEEEEHHHHHHHHCCCCCHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEECCCCC
SKVAVPAFENVNDKTNEGLEYVGKNIFTVQFHPEACAGPQDTAYLFDRFMDMMKKED
CEEECCCCCCCCCCCCCCHHHHCCEEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAFLILEDGTVFEGTSIGSTREVISEIVFNTSMTGYLEVLTDPSYAGQAVTMTYPLIGN
CCEEEEEECCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCC
YGVCYKDMESQKAWPDGYIVRELSRMASNFRNEDPIQKFLLDNDIPGICGIDTRALTKIL
CCCEECCHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
REKGTMNGMITTNENYNLDEVLPKIKEYSVKGVVRKVTCSEKYVLTGKGPKVALMDFGAK
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCEEEEEECCCH
KNIARSLNNRGCEVTVYPAYTKAEEILASNPDGIMLSNGPGDPKECVEIIEEIKKLYNSN
HHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
VPIFAICLGHQLMALATGADTHRLKYGHRGANHPVKDLKTGKCYISSQNHGYVVDESTLD
CCEEEEEHHHHHHHHCCCCCHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEECCCCC
SKVAVPAFENVNDKTNEGLEYVGKNIFTVQFHPEACAGPQDTAYLFDRFMDMMKKED
CEEECCCCCCCCCCCCCCHHHHCCEEEEEEECCHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]