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The map label for this gene is gltS [H]

Identifier: 238922138

GI number: 238922138

Start: 329332

End: 330525

Strand: Reverse

Name: gltS [H]

Synonym: EUBELI_20373

Alternate gene names: 238922138

Gene position: 330525-329332 (Counterclockwise)

Preceding gene: 238922139

Following gene: 238922137

Centisome position: 52.74

GC content: 36.26

Gene sequence:

>1194_bases
ATGAAGGTTCAGTTAGATATGTATCAGACGCTGGCTATTGCGGTACTTGTACTGCTTCTAGGCAGCTATTTACGAAAGAA
AATTAAATTCCTTGAAAGGTTCTGTATACCGGCACCTGTTGTAGGAGGACTGTTGTTTGCTATATTTACATGTATATGTT
ATGTTACTCATATATTAGAGTTTTCATTTGATGACACACTTAAAGAAGTATGCATGGTATTCTTTTTTACATCCGTAGGA
TTTCAGGCTAATCTTAAAGTACTTAAAAAGGGCGGAAAATCTATGGTGATATTTTTAGGATTAGTTATAGTACTTATATT
TATACAGAATGGTGTTGCAGTAGGTTTATCTAAGGTTATTGGGCTTGATTCACTTATAGGAATGTGTACAGGCTCAATAC
CTATGGTTGGCGGACATGGTACTGCGGGTGCATTTGGACCGGTACTTGAAGATTTTAATGTTAAGGGTGCAACGACAATA
TGTACTGCTGCAGCTACATTTGGTCTTATATTTGGAAGTCTTGTAGGAGGACCATTAGGCAAGAGACTTATTGAGAAGAA
AAACCTTTTAGATACAGCAATTCCAGAGGATGACAGTCTTCTTATTGAAGACGAAAAGAAGCATGAAAGACATTCCAGAA
TGTATGCATCAGCCGTATTCCAGCTAATTATTGCTATTGGTATAGGAACTATTTTTTCATGGGCTCTTACGAAGACAGGA
CTTACTTTTCCTATATACATCGGGGCCATGATTGCAGCTGCGCTTATGCGTAACATTGCAGAATATGCAGGAAATGGAAA
GCTTGTTATTCATATGGGAGAAATTAATGACCTTGGAGGTATAAGTTTATCATTATTTCTTGGAATGGCTATGATTACAC
TTAAGTTATGGCAGCTTGCATCACTAGCTCTTCCGCTTGTTATACTTCTTGGTGCACAGATAATTGTTATTGTTTTATTT
ACATATTTTATTGTATTCAATATTATGGGAAAAGATTACGATGCAGCAGTTCTTGTTGCAGGTACATGTGGATTTGGAAT
GGGAGCAACGCCTAATGCCATGGCAAATATGCAGGCAGTATGTGACAAATATGTTCCATCAGTTAAGGCTTATCTGTTAA
TACCACTTATAGGAAGTCTTTTTGCAGATTTCCTGAATAGTCTGGTTATAACTTTTTTTATCAATATATTATAA

Upstream 100 bases:

>100_bases
TCAGGTGCAGCAGCTTATTATACAGAGCGTGGCATCAATGTTTTGACAGAATAATTAATTCTGTATATCAGGTTAATGCT
ATAAAGAATGGAGAAATTTA

Downstream 100 bases:

>100_bases
TTATAGAGGAGTTAAATATGTTTAATATATTCAAAAAGGTAAAAAATAAAGTGAATACATCTGAAAAGGATTATAGTCTG
TCGTATGATAATCTTGCTAT

Product: glutamate:Na+ symporter, ESS family

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKVQLDMYQTLAIAVLVLLLGSYLRKKIKFLERFCIPAPVVGGLLFAIFTCICYVTHILEFSFDDTLKEVCMVFFFTSVG
FQANLKVLKKGGKSMVIFLGLVIVLIFIQNGVAVGLSKVIGLDSLIGMCTGSIPMVGGHGTAGAFGPVLEDFNVKGATTI
CTAAATFGLIFGSLVGGPLGKRLIEKKNLLDTAIPEDDSLLIEDEKKHERHSRMYASAVFQLIIAIGIGTIFSWALTKTG
LTFPIYIGAMIAAALMRNIAEYAGNGKLVIHMGEINDLGGISLSLFLGMAMITLKLWQLASLALPLVILLGAQIIVIVLF
TYFIVFNIMGKDYDAAVLVAGTCGFGMGATPNAMANMQAVCDKYVPSVKAYLLIPLIGSLFADFLNSLVITFFINIL

Sequences:

>Translated_397_residues
MKVQLDMYQTLAIAVLVLLLGSYLRKKIKFLERFCIPAPVVGGLLFAIFTCICYVTHILEFSFDDTLKEVCMVFFFTSVG
FQANLKVLKKGGKSMVIFLGLVIVLIFIQNGVAVGLSKVIGLDSLIGMCTGSIPMVGGHGTAGAFGPVLEDFNVKGATTI
CTAAATFGLIFGSLVGGPLGKRLIEKKNLLDTAIPEDDSLLIEDEKKHERHSRMYASAVFQLIIAIGIGTIFSWALTKTG
LTFPIYIGAMIAAALMRNIAEYAGNGKLVIHMGEINDLGGISLSLFLGMAMITLKLWQLASLALPLVILLGAQIIVIVLF
TYFIVFNIMGKDYDAAVLVAGTCGFGMGATPNAMANMQAVCDKYVPSVKAYLLIPLIGSLFADFLNSLVITFFINIL
>Mature_397_residues
MKVQLDMYQTLAIAVLVLLLGSYLRKKIKFLERFCIPAPVVGGLLFAIFTCICYVTHILEFSFDDTLKEVCMVFFFTSVG
FQANLKVLKKGGKSMVIFLGLVIVLIFIQNGVAVGLSKVIGLDSLIGMCTGSIPMVGGHGTAGAFGPVLEDFNVKGATTI
CTAAATFGLIFGSLVGGPLGKRLIEKKNLLDTAIPEDDSLLIEDEKKHERHSRMYASAVFQLIIAIGIGTIFSWALTKTG
LTFPIYIGAMIAAALMRNIAEYAGNGKLVIHMGEINDLGGISLSLFLGMAMITLKLWQLASLALPLVILLGAQIIVIVLF
TYFIVFNIMGKDYDAAVLVAGTCGFGMGATPNAMANMQAVCDKYVPSVKAYLLIPLIGSLFADFLNSLVITFFINIL

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=397, Percent_Identity=38.7909319899244, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 42685; Mature: 42685

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]