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The map label for this gene is lcfB [H]

Identifier: 238922095

GI number: 238922095

Start: 276721

End: 278184

Strand: Reverse

Name: lcfB [H]

Synonym: EUBELI_20330

Alternate gene names: 238922095

Gene position: 278184-276721 (Counterclockwise)

Preceding gene: 238922096

Following gene: 238922094

Centisome position: 44.39

GC content: 40.03

Gene sequence:

>1464_bases
ATGGTAGATACTGATGAAATGGCTACTTTGGAATATATTCCAACTATTCCAAAGTTTCTTGAATTTATTAAGAAGCAATA
TTCTGATTATCCAGCCATAAGTGACAAGGTTACTACATATACATATGAGGAGCTTGTTTCAAGAGTTGCTAAGAGAGTTA
CATTTATTAATTCATTAGGACTTGAGAAGGGTTCTAACATAGCAGTACTTGCAAAGAACGATTTAGATGCAATGGAGCTT
TTCCTTGCAATTCCAGCGGCTGGACATGTACTTATTATGCTTCCTAATGCACTTAACGATAAGGCACTTGCAGGAATCAG
CATGAAGTTTGACCTTGCTGGTATGTTCGTTGGCAAGGAATTTACAGAGGTTACAGCTAATCTTCCTTGTAAGACATGGC
CAAGCACTTCTATTGGTGAGACAGAAAGTGCATTTGCAGATGTTGAAAAGGATACTACAGCAGCCATTTTCTTTACAGGT
GGAACTACAGGAGCGCCTAAGGGTGCAGTTCACAGCCACGGTTCTATTATGAGGGGCTCATTTAATGGAGTATTTGGACC
GGGAAGTATTCTCCATAAGAGATATATAGCTATGCTTCCTCTTTCACACATATTTGGGGCAATTATGGGATATATGGCTA
AATTGTATACAGGTTCGCTTATATATACATGTGTTGATATGAGAGCCGGTATCGGTGATATTCCTGTTGTAAGACCTACA
ACGCTTGTACTTGTTCCTGGTCTTGTTGAGATTATCCTTAACATTGCCAGGCTTAAGGGAAGAGCATTCTTAGGAGATCT
TGAGCTTATCATGTGCGGAGCTGCACCTGTTCCTCCAAGACAGATGGCAGAGTGCCGTGAATTAGGAATTACATTATGCG
CGGGTTATGGACTTACAGAGTGTGCTAACCTGACATCAGGCAATTGCGATACAGATAAGAAGCCTGAATCTATGGGACAC
ATTTATCCAGAACAGCAGGTTAAGGTTGTTGAAGGAGAACTCTGGATTAAGGGTGATAATGTAATGAAGGAATATTACAA
GGATCCTGAGCATACAGCAGAAGTATTAGAAGACGGATGGTTTAAGACAGGTGACCTTGTTGAGTTTGATGATAATGACT
GGATATACATAACAGGTCGTATCAAGAATCTTATCATTCTTCCTAATGGTGAAAATGTATCGCCAGAAGAGATAGAGGAA
TTGTTCTACAAGCAGTCGCTTGTAAAGGATTGTCTTGTACAGGAGATGGAAATGAATGGAAACACTGTTATCGGTGTTGA
GATTATTCCATATATGCCTGAATTAGAGGGTGCATCTGAGGAAGAAATCCAGAACAGATTGCAGGAAACTGTTAATGAGG
TTAATAAGGAACTCCCACCATTCAAGAGAGTGCTTAAGCTTAAGGTGCGTACAGAAGATTTTAAGAGAAATGGCGCAATG
AAGATTTTGAGGAATCAGGCGTAA

Upstream 100 bases:

>100_bases
GGGCTTTTTCAAAACCGCGTACCGTCTGATTGCCAACACACCCCACCGGATTTCAATAAAGTAAAATAAGGAGCAAAGCA
TGAATAATTATTTTTATAAC

Downstream 100 bases:

>100_bases
GGTTGTGGTGGTGCCCGGAAGGTAGTGACAGGACGAACGGGGCTGCGGCTGGGTGTGACGCGGGCGTTGTGTGGGTGTGT
AGCATTTTGAAACACGCTTT

Product: long-chain fatty-acid-CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MVDTDEMATLEYIPTIPKFLEFIKKQYSDYPAISDKVTTYTYEELVSRVAKRVTFINSLGLEKGSNIAVLAKNDLDAMEL
FLAIPAAGHVLIMLPNALNDKALAGISMKFDLAGMFVGKEFTEVTANLPCKTWPSTSIGETESAFADVEKDTTAAIFFTG
GTTGAPKGAVHSHGSIMRGSFNGVFGPGSILHKRYIAMLPLSHIFGAIMGYMAKLYTGSLIYTCVDMRAGIGDIPVVRPT
TLVLVPGLVEIILNIARLKGRAFLGDLELIMCGAAPVPPRQMAECRELGITLCAGYGLTECANLTSGNCDTDKKPESMGH
IYPEQQVKVVEGELWIKGDNVMKEYYKDPEHTAEVLEDGWFKTGDLVEFDDNDWIYITGRIKNLIILPNGENVSPEEIEE
LFYKQSLVKDCLVQEMEMNGNTVIGVEIIPYMPELEGASEEEIQNRLQETVNEVNKELPPFKRVLKLKVRTEDFKRNGAM
KILRNQA

Sequences:

>Translated_487_residues
MVDTDEMATLEYIPTIPKFLEFIKKQYSDYPAISDKVTTYTYEELVSRVAKRVTFINSLGLEKGSNIAVLAKNDLDAMEL
FLAIPAAGHVLIMLPNALNDKALAGISMKFDLAGMFVGKEFTEVTANLPCKTWPSTSIGETESAFADVEKDTTAAIFFTG
GTTGAPKGAVHSHGSIMRGSFNGVFGPGSILHKRYIAMLPLSHIFGAIMGYMAKLYTGSLIYTCVDMRAGIGDIPVVRPT
TLVLVPGLVEIILNIARLKGRAFLGDLELIMCGAAPVPPRQMAECRELGITLCAGYGLTECANLTSGNCDTDKKPESMGH
IYPEQQVKVVEGELWIKGDNVMKEYYKDPEHTAEVLEDGWFKTGDLVEFDDNDWIYITGRIKNLIILPNGENVSPEEIEE
LFYKQSLVKDCLVQEMEMNGNTVIGVEIIPYMPELEGASEEEIQNRLQETVNEVNKELPPFKRVLKLKVRTEDFKRNGAM
KILRNQA
>Mature_487_residues
MVDTDEMATLEYIPTIPKFLEFIKKQYSDYPAISDKVTTYTYEELVSRVAKRVTFINSLGLEKGSNIAVLAKNDLDAMEL
FLAIPAAGHVLIMLPNALNDKALAGISMKFDLAGMFVGKEFTEVTANLPCKTWPSTSIGETESAFADVEKDTTAAIFFTG
GTTGAPKGAVHSHGSIMRGSFNGVFGPGSILHKRYIAMLPLSHIFGAIMGYMAKLYTGSLIYTCVDMRAGIGDIPVVRPT
TLVLVPGLVEIILNIARLKGRAFLGDLELIMCGAAPVPPRQMAECRELGITLCAGYGLTECANLTSGNCDTDKKPESMGH
IYPEQQVKVVEGELWIKGDNVMKEYYKDPEHTAEVLEDGWFKTGDLVEFDDNDWIYITGRIKNLIILPNGENVSPEEIEE
LFYKQSLVKDCLVQEMEMNGNTVIGVEIIPYMPELEGASEEEIQNRLQETVNEVNKELPPFKRVLKLKVRTEDFKRNGAM
KILRNQA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=396, Percent_Identity=27.5252525252525, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI57165412, Length=418, Percent_Identity=25.5980861244019, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI57165410, Length=418, Percent_Identity=25.3588516746411, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI42794760, Length=406, Percent_Identity=27.0935960591133, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI42794758, Length=406, Percent_Identity=27.0935960591133, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI156151445, Length=289, Percent_Identity=30.7958477508651, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI42794756, Length=406, Percent_Identity=27.0935960591133, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI27477105, Length=356, Percent_Identity=25.8426966292135, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI145693145, Length=421, Percent_Identity=26.3657957244656, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788107, Length=455, Percent_Identity=25.7142857142857, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789201, Length=292, Percent_Identity=28.0821917808219, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788595, Length=465, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=23.1683168316832, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786810, Length=288, Percent_Identity=26.3888888888889, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=344, Percent_Identity=31.6860465116279, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=345, Percent_Identity=27.2463768115942, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17556552, Length=347, Percent_Identity=27.0893371757925, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=355, Percent_Identity=27.0422535211268, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=293, Percent_Identity=28.6689419795222, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=447, Percent_Identity=24.6085011185682, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=447, Percent_Identity=24.6085011185682, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=398, Percent_Identity=28.391959798995, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17564090, Length=384, Percent_Identity=25.2604166666667, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17541856, Length=322, Percent_Identity=26.3975155279503, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=428, Percent_Identity=25.9345794392523, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17553312, Length=407, Percent_Identity=26.044226044226, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=381, Percent_Identity=25.7217847769029, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17510401, Length=318, Percent_Identity=24.8427672955975, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=303, Percent_Identity=27.7227722772277, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=305, Percent_Identity=26.8852459016393, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=300, Percent_Identity=27, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204819, Length=364, Percent_Identity=26.0989010989011, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324893, Length=339, Percent_Identity=30.0884955752212, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323903, Length=322, Percent_Identity=29.1925465838509, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322182, Length=328, Percent_Identity=29.2682926829268, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320852, Length=325, Percent_Identity=26.4615384615385, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=280, Percent_Identity=26.7857142857143, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281366413, Length=391, Percent_Identity=26.0869565217391, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24666501, Length=391, Percent_Identity=26.0869565217391, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24666497, Length=391, Percent_Identity=26.0869565217391, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21355181, Length=403, Percent_Identity=27.0471464019851, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581924, Length=272, Percent_Identity=27.5735294117647, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI18859661, Length=357, Percent_Identity=24.9299719887955, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17933690, Length=506, Percent_Identity=23.7154150197628, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648260, Length=271, Percent_Identity=26.1992619926199, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653035, Length=360, Percent_Identity=25.2777777777778, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076582, Length=365, Percent_Identity=25.2054794520548, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 53863; Mature: 53863

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTDEMATLEYIPTIPKFLEFIKKQYSDYPAISDKVTTYTYEELVSRVAKRVTFINSLG
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
LEKGSNIAVLAKNDLDAMELFLAIPAAGHVLIMLPNALNDKALAGISMKFDLAGMFVGKE
CCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHCCEEEEEEEHHHHHCCH
FTEVTANLPCKTWPSTSIGETESAFADVEKDTTAAIFFTGGTTGAPKGAVHSHGSIMRGS
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECC
FNGVFGPGSILHKRYIAMLPLSHIFGAIMGYMAKLYTGSLIYTCVDMRAGIGDIPVVRPT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
TLVLVPGLVEIILNIARLKGRAFLGDLELIMCGAAPVPPRQMAECRELGITLCAGYGLTE
CEEEHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHH
CANLTSGNCDTDKKPESMGHIYPEQQVKVVEGELWIKGDNVMKEYYKDPEHTAEVLEDGW
HCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECEEEEECCHHHHHHCCCHHHHHHHHHCCC
FKTGDLVEFDDNDWIYITGRIKNLIILPNGENVSPEEIEELFYKQSLVKDCLVQEMEMNG
CCCCCEEEECCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NTVIGVEIIPYMPELEGASEEEIQNRLQETVNEVNKELPPFKRVLKLKVRTEDFKRNGAM
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
KILRNQA
HHHCCCC
>Mature Secondary Structure
MVDTDEMATLEYIPTIPKFLEFIKKQYSDYPAISDKVTTYTYEELVSRVAKRVTFINSLG
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
LEKGSNIAVLAKNDLDAMELFLAIPAAGHVLIMLPNALNDKALAGISMKFDLAGMFVGKE
CCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHCCEEEEEEEHHHHHCCH
FTEVTANLPCKTWPSTSIGETESAFADVEKDTTAAIFFTGGTTGAPKGAVHSHGSIMRGS
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEECC
FNGVFGPGSILHKRYIAMLPLSHIFGAIMGYMAKLYTGSLIYTCVDMRAGIGDIPVVRPT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
TLVLVPGLVEIILNIARLKGRAFLGDLELIMCGAAPVPPRQMAECRELGITLCAGYGLTE
CEEEHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCCHHHHHHHHHCCEEEECCCCHHH
CANLTSGNCDTDKKPESMGHIYPEQQVKVVEGELWIKGDNVMKEYYKDPEHTAEVLEDGW
HCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECEEEEECCHHHHHHCCCHHHHHHHHHCCC
FKTGDLVEFDDNDWIYITGRIKNLIILPNGENVSPEEIEELFYKQSLVKDCLVQEMEMNG
CCCCCEEEECCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
NTVIGVEIIPYMPELEGASEEEIQNRLQETVNEVNKELPPFKRVLKLKVRTEDFKRNGAM
CEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCH
KILRNQA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]