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The map label for this gene is pcrA [H]

Identifier: 238921998

GI number: 238921998

Start: 192203

End: 192772

Strand: Reverse

Name: pcrA [H]

Synonym: EUBELI_20233

Alternate gene names: 238921998

Gene position: 192772-192203 (Counterclockwise)

Preceding gene: 238922000

Following gene: 238921997

Centisome position: 30.76

GC content: 36.14

Gene sequence:

>570_bases
ATGATAATAACTCATATTAAGAAGACATATGTTATATTTATATGTCTGAAAGATGTCTTTGGTAAAAATCTTCCAATCTA
CACATTTAACAATATCTGTGAACAGGACCACTCCTTGACACTTGGTGATGAAGCAACAAAGGTAATTGTAAATGCAGAAG
GTTCAAGGGACGATTTATCTGAGAATATGCGTAGTTTCTTGGATTCTCTGATTGAAAAAAAGGCAACGAAGGAACAGACA
GAGGCAATAACACAGACAGAAGGCAACATAAGGGTTGCGTCAGTTCCAGGTTCGGGAAAAACATTTGTACTTACATATAG
AATAGCATATCTCATAACAGAACTGTTTGTTGAGCCATCATCTATTGTGGCGCTTACATTTACAAACAAGGCAGCTTCGC
AGATGAAGCACAGATTATGGAAGATAAAAAATATTCACTGGGTGGGTTTACATTTGATACGCAGCAGGAATATGAGCGGG
CAAAAGTTGAATTGCAGGTTATTCTTAAGATTAAACAAAAATATGATATTAATAATCCAGAGGATGCCAAAAATGTTTTG
GATGCCGTGA

Upstream 100 bases:

>100_bases
TTAGATAACAAACTATTCATATACTATATAAGATTATTGATATCAATATTCATATAAATGTGGTAATATAATAGTGTAAT
TATATTAGTATTATCTAGTA

Downstream 100 bases:

>100_bases
ATAAAAAAGGAGATGTGTTTAAGTCATCAGTTGGAAAAGCATTTATTAATAAGTTAAAAAGAGTGTCAAAGAATGAAAAT
GAAATACATGTACAAAAACC

Product: DNA helicase II / ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MIITHIKKTYVIFICLKDVFGKNLPIYTFNNICEQDHSLTLGDEATKVIVNAEGSRDDLSENMRSFLDSLIEKKATKEQT
EAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAASQMKHRLWKIKNIHWVGLHLIRSRNMSG
QKLNCRLFLRLNKNMILIIQRMPKMFWMP

Sequences:

>Translated_189_residues
MIITHIKKTYVIFICLKDVFGKNLPIYTFNNICEQDHSLTLGDEATKVIVNAEGSRDDLSENMRSFLDSLIEKKATKEQT
EAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAASQMKHRLWKIKNIHWVGLHLIRSRNMSG
QKLNCRLFLRLNKNMILIIQRMPKMFWMP
>Mature_189_residues
MIITHIKKTYVIFICLKDVFGKNLPIYTFNNICEQDHSLTLGDEATKVIVNAEGSRDDLSENMRSFLDSLIEKKATKEQT
EAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAASQMKHRLWKIKNIHWVGLHLIRSRNMSG
QKLNCRLFLRLNKNMILIIQRMPKMFWMP

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=127, Percent_Identity=37.7952755905512, Blast_Score=68, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322369, Length=73, Percent_Identity=41.0958904109589, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 21778; Mature: 21778

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITHIKKTYVIFICLKDVFGKNLPIYTFNNICEQDHSLTLGDEATKVIVNAEGSRDDLS
CEEEEEHHEEEEEEEEHHHHCCCCCEEEEHHHHCCCCCEEECCCCEEEEEECCCCHHHHH
ENMRSFLDSLIEKKATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCC
SIVALTFTNKAASQMKHRLWKIKNIHWVGLHLIRSRNMSGQKLNCRLFLRLNKNMILIIQ
CEEEEEECCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEEEEEEEEECCCEEEEEE
RMPKMFWMP
CCCHHHCCC
>Mature Secondary Structure
MIITHIKKTYVIFICLKDVFGKNLPIYTFNNICEQDHSLTLGDEATKVIVNAEGSRDDLS
CEEEEEHHEEEEEEEEHHHHCCCCCEEEEHHHHCCCCCEEECCCCEEEEEECCCCHHHHH
ENMRSFLDSLIEKKATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHCCCC
SIVALTFTNKAASQMKHRLWKIKNIHWVGLHLIRSRNMSGQKLNCRLFLRLNKNMILIIQ
CEEEEEECCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCEEEEEEEEEECCCEEEEEE
RMPKMFWMP
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]