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The map label for this gene is cobQ [H]

Identifier: 238921939

GI number: 238921939

Start: 137186

End: 138700

Strand: Reverse

Name: cobQ [H]

Synonym: EUBELI_20173

Alternate gene names: 238921939

Gene position: 138700-137186 (Counterclockwise)

Preceding gene: 238921940

Following gene: 238921938

Centisome position: 22.13

GC content: 40.33

Gene sequence:

>1515_bases
ATGGCAAAGAATCTTATGATTCAGGGAACAATGTCTAATGCAGGAAAGAGCATTATAACGGCAGGAATATTAAGAGTGCT
GAAGCAGGACGGGTATAATGCAGCACCTTTTAAATCACAGAATATGGCATTGAATTCATACATAACAGCGGATGGTCTGG
AAATGGGAAGAGCACAGGTTATGCAGGCAGAAGCGGCAGGAATAGCACCATGTGTAGAAATGAATCCCATATTGTTAAAG
CCGACAAGCGATGTGGGTTCACAGGTTATTGTAAACGGAACACCGCTTAAAAATATGCCGGCTAAAGAATATTTCCAGTA
TAAAAAGAAACTGATTCCTGACATATTAAAAGCCTATGACACTCTTGACAGAAAATATGATGTGATAGTACTTGAGGGCG
CAGGAAGTCCGGCAGAGATTAATCTTAAGAAAGATGATATTGTTAATATGGGAATGGCGGAGCTTGTAGATGCACCGGTG
CTTCTTGTTGGAGATATTGACCGTGGTGGCGTGTTTGCGCAGCTTGTTGGAACAATCATGCTTCTTGAAGAAAAGGAACG
CAGGCGTGTAAAAGGTCTGGTCATGAATAAGTTCCGTGGAGACAGGAAGATACTTGAGCCAGGTATAAAACAGTTATATG
ACATATGTCCGATTCCTGTTGCCGGGGTAATTCCTTATGTAAGGCTTGATATTGAAGATGAAGACAGCCTTGCTTCAAGG
CTTGAAACATCGGACTCAGATGTTTCAGGGGAAAAGCTGGATATAGTTGTAATAAGACTGCCTCATATTTCGAATTTTAC
AGATTTTAATGCACTCGAAAGGCTGCCGGAGGTTAATTTAAGATATGTCGATAAGGCAGAACAGATAGGAAATCCCAATC
TTATAATTCTTCCGGGAAGCAAGAATACCATTAATGATTTAAAATGGCTGCGTAAGAATGGAATTGAAGCACAGATTATA
AAATGTGCAAGGCAGGAGATACCTGTATTTGGAATATGTGGTGGTTACCAGATGTTAGGAAATGTTATTGATGATTCATG
TAATGCAGAAACCGGTGAGGTTATTCCGGGAATAGGCCTTTTACCAGTTACAACTACATTTACACTGTCTAAGCACCGTA
CAAGAGCAAGAGGAATAATCTGCAGCCAGTCTGGAATGTGGGAAATGCTTGGGAAGAAAAAATGTGCCGGATATGAAATT
CATATGGGAGAGAGTACTGTGGGTGAAGGACAGGCGCATGCATTTGCAAAAATATATAATACTGTCGATGGAACGGAATA
TATGGATGGGTGTGTCTGTGGGAATGTGGCAGGAACTTATCTTCACGGAATATTTGATGAGAAAGAATTCTGTGACAGTT
TTATAAATATGCTTGGTAAAAGATGTGGGATTGCGATACACGGCGGTTCAGACATGAGTTTTGAAGAATATAAGCAGAGA
GAGTATGACAAGCTGGCAGATGCAATCCGTGAAAATATGGATATGAAGTATATATATGAAATTATGGGATTGTAG

Upstream 100 bases:

>100_bases
GCTACCGTATAGCAGTAAAGCAGCATGCGGATAATGAAGTTCTTATTAATAATATGAGACAGATACTGGAAGTGTAAAAG
AAAGGCAGGGTGCAGTGATT

Downstream 100 bases:

>100_bases
ACAGCAGGAAGGGAGATACATTATATGAATAGTATAAAGAAGGCATATTACCATCTTCCGGGGTTGTTTGAATTCTATGA
ATTGTACGAAGCATTTTTGC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAKNLMIQGTMSNAGKSIITAGILRVLKQDGYNAAPFKSQNMALNSYITADGLEMGRAQVMQAEAAGIAPCVEMNPILLK
PTSDVGSQVIVNGTPLKNMPAKEYFQYKKKLIPDILKAYDTLDRKYDVIVLEGAGSPAEINLKKDDIVNMGMAELVDAPV
LLVGDIDRGGVFAQLVGTIMLLEEKERRRVKGLVMNKFRGDRKILEPGIKQLYDICPIPVAGVIPYVRLDIEDEDSLASR
LETSDSDVSGEKLDIVVIRLPHISNFTDFNALERLPEVNLRYVDKAEQIGNPNLIILPGSKNTINDLKWLRKNGIEAQII
KCARQEIPVFGICGGYQMLGNVIDDSCNAETGEVIPGIGLLPVTTTFTLSKHRTRARGIICSQSGMWEMLGKKKCAGYEI
HMGESTVGEGQAHAFAKIYNTVDGTEYMDGCVCGNVAGTYLHGIFDEKEFCDSFINMLGKRCGIAIHGGSDMSFEEYKQR
EYDKLADAIRENMDMKYIYEIMGL

Sequences:

>Translated_504_residues
MAKNLMIQGTMSNAGKSIITAGILRVLKQDGYNAAPFKSQNMALNSYITADGLEMGRAQVMQAEAAGIAPCVEMNPILLK
PTSDVGSQVIVNGTPLKNMPAKEYFQYKKKLIPDILKAYDTLDRKYDVIVLEGAGSPAEINLKKDDIVNMGMAELVDAPV
LLVGDIDRGGVFAQLVGTIMLLEEKERRRVKGLVMNKFRGDRKILEPGIKQLYDICPIPVAGVIPYVRLDIEDEDSLASR
LETSDSDVSGEKLDIVVIRLPHISNFTDFNALERLPEVNLRYVDKAEQIGNPNLIILPGSKNTINDLKWLRKNGIEAQII
KCARQEIPVFGICGGYQMLGNVIDDSCNAETGEVIPGIGLLPVTTTFTLSKHRTRARGIICSQSGMWEMLGKKKCAGYEI
HMGESTVGEGQAHAFAKIYNTVDGTEYMDGCVCGNVAGTYLHGIFDEKEFCDSFINMLGKRCGIAIHGGSDMSFEEYKQR
EYDKLADAIRENMDMKYIYEIMGL
>Mature_503_residues
AKNLMIQGTMSNAGKSIITAGILRVLKQDGYNAAPFKSQNMALNSYITADGLEMGRAQVMQAEAAGIAPCVEMNPILLKP
TSDVGSQVIVNGTPLKNMPAKEYFQYKKKLIPDILKAYDTLDRKYDVIVLEGAGSPAEINLKKDDIVNMGMAELVDAPVL
LVGDIDRGGVFAQLVGTIMLLEEKERRRVKGLVMNKFRGDRKILEPGIKQLYDICPIPVAGVIPYVRLDIEDEDSLASRL
ETSDSDVSGEKLDIVVIRLPHISNFTDFNALERLPEVNLRYVDKAEQIGNPNLIILPGSKNTINDLKWLRKNGIEAQIIK
CARQEIPVFGICGGYQMLGNVIDDSCNAETGEVIPGIGLLPVTTTFTLSKHRTRARGIICSQSGMWEMLGKKKCAGYEIH
MGESTVGEGQAHAFAKIYNTVDGTEYMDGCVCGNVAGTYLHGIFDEKEFCDSFINMLGKRCGIAIHGGSDMSFEEYKQRE
YDKLADAIRENMDMKYIYEIMGL

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 55561; Mature: 55430

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNLMIQGTMSNAGKSIITAGILRVLKQDGYNAAPFKSQNMALNSYITADGLEMGRAQV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
MQAEAAGIAPCVEMNPILLKPTSDVGSQVIVNGTPLKNMPAKEYFQYKKKLIPDILKAYD
HHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLDRKYDVIVLEGAGSPAEINLKKDDIVNMGMAELVDAPVLLVGDIDRGGVFAQLVGTIM
HHCCCEEEEEEECCCCCCEEEECHHHHHCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHH
LLEEKERRRVKGLVMNKFRGDRKILEPGIKQLYDICPIPVAGVIPYVRLDIEDEDSLASR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHH
LETSDSDVSGEKLDIVVIRLPHISNFTDFNALERLPEVNLRYVDKAEQIGNPNLIILPGS
HCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCEEEECHHHHCCCCCEEEECCC
KNTINDLKWLRKNGIEAQIIKCARQEIPVFGICGGYQMLGNVIDDSCNAETGEVIPGIGL
CCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCE
LPVTTTFTLSKHRTRARGIICSQSGMWEMLGKKKCAGYEIHMGESTVGEGQAHAFAKIYN
EECEEEEEEHHHHHHHCCEEECCCCHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
TVDGTEYMDGCVCGNVAGTYLHGIFDEKEFCDSFINMLGKRCGIAIHGGSDMSFEEYKQR
CCCCCHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
EYDKLADAIRENMDMKYIYEIMGL
HHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
AKNLMIQGTMSNAGKSIITAGILRVLKQDGYNAAPFKSQNMALNSYITADGLEMGRAQV
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHH
MQAEAAGIAPCVEMNPILLKPTSDVGSQVIVNGTPLKNMPAKEYFQYKKKLIPDILKAYD
HHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TLDRKYDVIVLEGAGSPAEINLKKDDIVNMGMAELVDAPVLLVGDIDRGGVFAQLVGTIM
HHCCCEEEEEEECCCCCCEEEECHHHHHCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHH
LLEEKERRRVKGLVMNKFRGDRKILEPGIKQLYDICPIPVAGVIPYVRLDIEDEDSLASR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHCCCEEEEECCCCHHHHHH
LETSDSDVSGEKLDIVVIRLPHISNFTDFNALERLPEVNLRYVDKAEQIGNPNLIILPGS
HCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCEEEECHHHHCCCCCEEEECCC
KNTINDLKWLRKNGIEAQIIKCARQEIPVFGICGGYQMLGNVIDDSCNAETGEVIPGIGL
CCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCE
LPVTTTFTLSKHRTRARGIICSQSGMWEMLGKKKCAGYEIHMGESTVGEGQAHAFAKIYN
EECEEEEEEHHHHHHHCCEEECCCCHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHH
TVDGTEYMDGCVCGNVAGTYLHGIFDEKEFCDSFINMLGKRCGIAIHGGSDMSFEEYKQR
CCCCCHHHCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHH
EYDKLADAIRENMDMKYIYEIMGL
HHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA