Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 238921901

GI number: 238921901

Start: 112644

End: 113126

Strand: Reverse

Name: queF [H]

Synonym: EUBELI_20135

Alternate gene names: 238921901

Gene position: 113126-112644 (Counterclockwise)

Preceding gene: 238921902

Following gene: 238921899

Centisome position: 18.05

GC content: 40.37

Gene sequence:

>483_bases
ATGAGAGAAAAAGAGAATTTAACATTATTAGGCAACCAGCAGAATAATTACGAGACAGATTACAACCCTGGCCTGCTTGA
AACATTTGTAAACAAACATCCAGAAAATGATTATTTCGTGAAGTTTAACTGCCCGGAATTCACAAGTCTCTGTCCAATTA
CAGGGCAGCCTGATTTCGCAAACATTATTATTTCGTATGTCCCAGGCGAGAGAATGGTGGAAAGCAAGAGCTTAAAGCTG
TACCTGTTTTCATTCCGTAATCATGGGGATTTTCACGAGGACTGCGTGAACGTGATTATGAAGGATTTGATTACGCTGAT
GGAGCCAAAGTATATTGAAGTGTGGGGCAAATTCACACCACGTGGTGGAATTTCCATTGATCCATACTGTAATTATGGAA
AGCCAGGAACTAAGTGGGAAGATGTGGCATTCAACCGTATGGCAAATCATGATATGTATCCAGAGAAGGTGGATAATAGG
TAA

Upstream 100 bases:

>100_bases
TGTGCCTTGCGGCAAATGTGGAACCTGTATCGACAGAGCGGCTGCATTTGCAGCAAATGGGGTAAGTGACCCTGCAATAA
AATAAAAATTGGAGAAAGCT

Downstream 100 bases:

>100_bases
AATAACATTACTAAATTTCGTGCAATGCACAAAAACCAGAAGAGTGTTGTAAGGTAATAAAAGATTAAGCGATATTGTAT
GAATTTACTTCACACAATAT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MREKENLTLLGNQQNNYETDYNPGLLETFVNKHPENDYFVKFNCPEFTSLCPITGQPDFANIIISYVPGERMVESKSLKL
YLFSFRNHGDFHEDCVNVIMKDLITLMEPKYIEVWGKFTPRGGISIDPYCNYGKPGTKWEDVAFNRMANHDMYPEKVDNR

Sequences:

>Translated_160_residues
MREKENLTLLGNQQNNYETDYNPGLLETFVNKHPENDYFVKFNCPEFTSLCPITGQPDFANIIISYVPGERMVESKSLKL
YLFSFRNHGDFHEDCVNVIMKDLITLMEPKYIEVWGKFTPRGGISIDPYCNYGKPGTKWEDVAFNRMANHDMYPEKVDNR
>Mature_160_residues
MREKENLTLLGNQQNNYETDYNPGLLETFVNKHPENDYFVKFNCPEFTSLCPITGQPDFANIIISYVPGERMVESKSLKL
YLFSFRNHGDFHEDCVNVIMKDLITLMEPKYIEVWGKFTPRGGISIDPYCNYGKPGTKWEDVAFNRMANHDMYPEKVDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=79, Percent_Identity=40.5063291139241, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 18690; Mature: 18690

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKENLTLLGNQQNNYETDYNPGLLETFVNKHPENDYFVKFNCPEFTSLCPITGQPDFA
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHH
NIIISYVPGERMVESKSLKLYLFSFRNHGDFHEDCVNVIMKDLITLMEPKYIEVWGKFTP
HEEEEECCCCHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RGGISIDPYCNYGKPGTKWEDVAFNRMANHDMYPEKVDNR
CCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MREKENLTLLGNQQNNYETDYNPGLLETFVNKHPENDYFVKFNCPEFTSLCPITGQPDFA
CCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHH
NIIISYVPGERMVESKSLKLYLFSFRNHGDFHEDCVNVIMKDLITLMEPKYIEVWGKFTP
HEEEEECCCCHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RGGISIDPYCNYGKPGTKWEDVAFNRMANHDMYPEKVDNR
CCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA