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The map label for this gene is asnA

Identifier: 238921731

GI number: 238921731

Start: 3778565

End: 3779557

Strand: Reverse

Name: asnA

Synonym: NT01EI_3900

Alternate gene names: 238921731

Gene position: 3779557-3778565 (Counterclockwise)

Preceding gene: 238921747

Following gene: 238921728

Centisome position: 99.14

GC content: 57.5

Gene sequence:

>993_bases
ATGAAAAAATCCTATATTGAAAAACAGCAGCAGATCAGCTTTGTTAAGGCACATTTCTCCCGTCAGCTGGAGCAGCGTCT
CGGTCTCATTGAAGTGCAGGCGCCGATCCTGAGCCGTTTGGGCGACGGTACGCAGGATAACCTTTCCGGTCATGAGAAGG
CGGTACAGGTAAAGGTGAAGAACCTGCCGCAGGACGCCTTTGAAGTGGTGCATTCTCTGGCCAAATGGAAACGTAAGACG
CTGGGAATGTATGACTTTGCACCGGGGGAAGGACTGTATACCCACATGAAGGCGCTGCGTCCCGATGAGGACCGTCTCTC
AGCGATCCACTCGGTGTACGTGGATCAATGGGACTGGGAGCGCGTGATGGGCGATGGGGAGCGTACCCCTGCCTATCTGC
AGGAGACGGTGCGCCACATTTATGCCGCGATGAAAACAACAGAGGCTGAAGTGTGTGCGCAGTACGGTTTAACGCCATTT
TTACCCGCGGAAATCCATTTTATTCACAGTGAGGCTCTGTTACGCCGTTATCCGGATCTGGATGCCAAAGGCCGCGAGCG
CGCGATCACCCATGAGCTGGGCGCCGTGTTCCTGATCGGGATCGGCGGCCGCTTGTCAAACGGTCAGGCCCATGACGTTC
GCGCGCCGGACTATGATGACTGGACGTCGCTGGGCGCAGAGGGCTTCGGTGGGCTCAACGGCGATATTCTGGTGTGGAAC
CCGGTGCTGGGTGATGCCTTTGAGCTCTCATCCATGGGGATCCGCGTTGATGCCAAGGCGCTGAAGCGTCAGTTGGCGCT
GACGGACGATGAGGATCGTCTGGCGCTGGAGTGGCATCAGTCACTGCTGCGCGGAGATATGCCACAGACTATCGGTGGCG
GTATCGGCCAATCCCGTCTGGTGATGCTGCTGCTGCAGCAGCAGCACATCGGTCAGGTGCAGTGCGGTGTATGGCCTCAG
GAGATTCGCCAACGCGTGCAGGCGATTCTCTAA

Upstream 100 bases:

>100_bases
AACCAATAAGGCAATATTTGTTGTTGATTATTGAATTCATCGTCGCATCTGTTGCTTAATCAATATCAACAGGAAAGAAA
GACAGGCAGTGAGTACAACA

Downstream 100 bases:

>100_bases
GACTCAATGGCGCCAACGACGCAGCAGACGTCCCTTAATTCCGCTGTCGAAGCGCCAAATATAATCAAAGACTTTCATTA
TGCTGGGCTTGCCATGTGGC

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKSYIEKQQQISFVKAHFSRQLEQRLGLIEVQAPILSRLGDGTQDNLSGHEKAVQVKVKNLPQDAFEVVHSLAKWKRKT
LGMYDFAPGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERTPAYLQETVRHIYAAMKTTEAEVCAQYGLTPF
LPAEIHFIHSEALLRRYPDLDAKGRERAITHELGAVFLIGIGGRLSNGQAHDVRAPDYDDWTSLGAEGFGGLNGDILVWN
PVLGDAFELSSMGIRVDAKALKRQLALTDDEDRLALEWHQSLLRGDMPQTIGGGIGQSRLVMLLLQQQHIGQVQCGVWPQ
EIRQRVQAIL

Sequences:

>Translated_330_residues
MKKSYIEKQQQISFVKAHFSRQLEQRLGLIEVQAPILSRLGDGTQDNLSGHEKAVQVKVKNLPQDAFEVVHSLAKWKRKT
LGMYDFAPGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERTPAYLQETVRHIYAAMKTTEAEVCAQYGLTPF
LPAEIHFIHSEALLRRYPDLDAKGRERAITHELGAVFLIGIGGRLSNGQAHDVRAPDYDDWTSLGAEGFGGLNGDILVWN
PVLGDAFELSSMGIRVDAKALKRQLALTDDEDRLALEWHQSLLRGDMPQTIGGGIGQSRLVMLLLQQQHIGQVQCGVWPQ
EIRQRVQAIL
>Mature_330_residues
MKKSYIEKQQQISFVKAHFSRQLEQRLGLIEVQAPILSRLGDGTQDNLSGHEKAVQVKVKNLPQDAFEVVHSLAKWKRKT
LGMYDFAPGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWERVMGDGERTPAYLQETVRHIYAAMKTTEAEVCAQYGLTPF
LPAEIHFIHSEALLRRYPDLDAKGRERAITHELGAVFLIGIGGRLSNGQAHDVRAPDYDDWTSLGAEGFGGLNGDILVWN
PVLGDAFELSSMGIRVDAKALKRQLALTDDEDRLALEWHQSLLRGDMPQTIGGGIGQSRLVMLLLQQQHIGQVQCGVWPQ
EIRQRVQAIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=330, Percent_Identity=76.3636363636364, Blast_Score=521, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_EDWI9 (C5BDG9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935246.1
- GeneID:   7961466
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3900
- OMA:   LNDNLNG
- ProtClustDB:   PRK05425
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 37127; Mature: 37127

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSYIEKQQQISFVKAHFSRQLEQRLGLIEVQAPILSRLGDGTQDNLSGHEKAVQVKVK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCHHHEEEHHH
NLPQDAFEVVHSLAKWKRKTLGMYDFAPGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RVMGDGERTPAYLQETVRHIYAAMKTTEAEVCAQYGLTPFLPAEIHFIHSEALLRRYPDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
DAKGRERAITHELGAVFLIGIGGRLSNGQAHDVRAPDYDDWTSLGAEGFGGLNGDILVWN
CCCCCHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEE
PVLGDAFELSSMGIRVDAKALKRQLALTDDEDRLALEWHQSLLRGDMPQTIGGGIGQSRL
CCCCCHHHHHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHH
VMLLLQQQHIGQVQCGVWPQEIRQRVQAIL
HHHHHHHHHCCCEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKKSYIEKQQQISFVKAHFSRQLEQRLGLIEVQAPILSRLGDGTQDNLSGHEKAVQVKVK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCHHHEEEHHH
NLPQDAFEVVHSLAKWKRKTLGMYDFAPGEGLYTHMKALRPDEDRLSAIHSVYVDQWDWE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
RVMGDGERTPAYLQETVRHIYAAMKTTEAEVCAQYGLTPFLPAEIHFIHSEALLRRYPDL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
DAKGRERAITHELGAVFLIGIGGRLSNGQAHDVRAPDYDDWTSLGAEGFGGLNGDILVWN
CCCCCHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEE
PVLGDAFELSSMGIRVDAKALKRQLALTDDEDRLALEWHQSLLRGDMPQTIGGGIGQSRL
CCCCCHHHHHHCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCHHHH
VMLLLQQQHIGQVQCGVWPQEIRQRVQAIL
HHHHHHHHHCCCEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA