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The map label for this gene is rbsB [H]

Identifier: 238921724

GI number: 238921724

Start: 3769438

End: 3770325

Strand: Reverse

Name: rbsB [H]

Synonym: NT01EI_3893

Alternate gene names: 238921724

Gene position: 3770325-3769438 (Counterclockwise)

Preceding gene: 238921725

Following gene: 238921723

Centisome position: 98.9

GC content: 58.56

Gene sequence:

>888_bases
ATGAATATTACAAAACTGGCAACCTTGATCTCCGCCGTGGCCCTGAGCGCCATCCTGAGTGCCAACGCCATGGCGCAGGA
TACCATTGCCCTGGTGGTCTCTACCCTGAATAACCCGTTCTTTGTGTCGATGAAGGATGGCGCGCAGAAAGAGGCCAATA
AGCTGGGATACAAGCTGGTGGTATTGGACTCACAGAACAACCCGGCCAAAGAGCTGGCCAACGTACAGGATCTGACCGTG
CGCGGCGCTAAGCTGATGCTGATTAACCCGACGGACTCCGATGCGGTGGGCAACGCGGTAAAAATGGCCAATCAGGCCAA
TATTCCGGTGATCACCCTTGACCGTGCGGCCAGCCAAGGTAGCGTGGTTAGCCATATCGCCTCCGATAACGTCGCCGGCG
GCAAGCTGGCCGGTGACTTTATCGCCCAGCAGCTGGGGACCGGTGCCAAGATCATTCAGCTGGAGGGGATCGCCGGAACC
TCGGCAGCCCGTGAGCGCGGCGAAGGCTTTAAACAGGCGGTCGTCGCCCATAAGTTTGACGTACTGGCCAGCCAGCCAGC
CGACTTTGACCGTACTAAGGGCTTGAACGTCATGCAAAACCTGCTCACGGCTCACCCGCAGGTTCAGGCCGTATTCGCGC
AGAACGATGAGATGGCGCTGGGTGCCCTGCGCGCTTTGCAAACGGCGGGGCGTAACGATGTGTTGGTCGTCGGATTCGAT
GGCACCCAAGACGGTGAAAAAGCGGTACAGAGCGGTAAGCTGGGAGCCACTGTAGCACAGCAGCCGGAGCAGATCGGCGT
GATCGGCGTCGAGACCGCGGATAAGGTGATCAAAGGGGAAAACGTGCCGGCCAACATTCCGGTTGAGCTGAAGCTGGTCA
CCAAGTAA

Upstream 100 bases:

>100_bases
CCTCCTACTACCAAATGATCGTCAAGGCAGCGGTGATTCTGTTGGCGGTTTTGGTAGACAACAAAAGCAATAAATAATCT
TACCTACAGGAATCGATGAG

Downstream 100 bases:

>100_bases
CGTACCGGGAGACCCTCCGGGCAACGCCGCCGCTGGGATACCGGCGGCGGTAGATAAGTAGGAATACAGAATATGCAAAC
AGGCAAACTGGTCGTATTAG

Product: D-ribose transporter subunit RbsB

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNITKLATLISAVALSAILSANAMAQDTIALVVSTLNNPFFVSMKDGAQKEANKLGYKLVVLDSQNNPAKELANVQDLTV
RGAKLMLINPTDSDAVGNAVKMANQANIPVITLDRAASQGSVVSHIASDNVAGGKLAGDFIAQQLGTGAKIIQLEGIAGT
SAARERGEGFKQAVVAHKFDVLASQPADFDRTKGLNVMQNLLTAHPQVQAVFAQNDEMALGALRALQTAGRNDVLVVGFD
GTQDGEKAVQSGKLGATVAQQPEQIGVIGVETADKVIKGENVPANIPVELKLVTK

Sequences:

>Translated_295_residues
MNITKLATLISAVALSAILSANAMAQDTIALVVSTLNNPFFVSMKDGAQKEANKLGYKLVVLDSQNNPAKELANVQDLTV
RGAKLMLINPTDSDAVGNAVKMANQANIPVITLDRAASQGSVVSHIASDNVAGGKLAGDFIAQQLGTGAKIIQLEGIAGT
SAARERGEGFKQAVVAHKFDVLASQPADFDRTKGLNVMQNLLTAHPQVQAVFAQNDEMALGALRALQTAGRNDVLVVGFD
GTQDGEKAVQSGKLGATVAQQPEQIGVIGVETADKVIKGENVPANIPVELKLVTK
>Mature_295_residues
MNITKLATLISAVALSAILSANAMAQDTIALVVSTLNNPFFVSMKDGAQKEANKLGYKLVVLDSQNNPAKELANVQDLTV
RGAKLMLINPTDSDAVGNAVKMANQANIPVITLDRAASQGSVVSHIASDNVAGGKLAGDFIAQQLGTGAKIIQLEGIAGT
SAARERGEGFKQAVVAHKFDVLASQPADFDRTKGLNVMQNLLTAHPQVQAVFAQNDEMALGALRALQTAGRNDVLVVGFD
GTQDGEKAVQSGKLGATVAQQPEQIGVIGVETADKVIKGENVPANIPVELKLVTK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=295, Percent_Identity=83.0508474576271, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1790526, Length=307, Percent_Identity=34.2019543973941, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790674, Length=230, Percent_Identity=30.8695652173913, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790194, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788473, Length=277, Percent_Identity=24.9097472924188, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 30699; Mature: 30699

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITKLATLISAVALSAILSANAMAQDTIALVVSTLNNPFFVSMKDGAQKEANKLGYKLV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCEEEE
VLDSQNNPAKELANVQDLTVRGAKLMLINPTDSDAVGNAVKMANQANIPVITLDRAASQG
EEECCCCCHHHHCCCHHEEECCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCCC
SVVSHIASDNVAGGKLAGDFIAQQLGTGAKIIQLEGIAGTSAARERGEGFKQAVVAHKFD
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHH
VLASQPADFDRTKGLNVMQNLLTAHPQVQAVFAQNDEMALGALRALQTAGRNDVLVVGFD
HHHCCCCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEC
GTQDGEKAVQSGKLGATVAQQPEQIGVIGVETADKVIKGENVPANIPVELKLVTK
CCCCHHHHHHCCCCCCHHHCCCCCCCEEEECCHHHHHCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MNITKLATLISAVALSAILSANAMAQDTIALVVSTLNNPFFVSMKDGAQKEANKLGYKLV
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCEEEE
VLDSQNNPAKELANVQDLTVRGAKLMLINPTDSDAVGNAVKMANQANIPVITLDRAASQG
EEECCCCCHHHHCCCHHEEECCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEECCCCCCC
SVVSHIASDNVAGGKLAGDFIAQQLGTGAKIIQLEGIAGTSAARERGEGFKQAVVAHKFD
HHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHH
VLASQPADFDRTKGLNVMQNLLTAHPQVQAVFAQNDEMALGALRALQTAGRNDVLVVGFD
HHHCCCCCCCHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEC
GTQDGEKAVQSGKLGATVAQQPEQIGVIGVETADKVIKGENVPANIPVELKLVTK
CCCCHHHHHHCCCCCCHHHCCCCCCCEEEECCHHHHHCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]