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The map label for this gene is rbsR [H]

Identifier: 238921722

GI number: 238921722

Start: 3767422

End: 3768429

Strand: Reverse

Name: rbsR [H]

Synonym: NT01EI_3890

Alternate gene names: 238921722

Gene position: 3768429-3767422 (Counterclockwise)

Preceding gene: 238921723

Following gene: 238921719

Centisome position: 98.85

GC content: 58.04

Gene sequence:

>1008_bases
GTGGCAACAATGAAGGACGTCGCCCGTCTCGCAGGCGTCTCCACCTCGACTGTATCCCATGTCATCAATAACAATCGCTT
CGTCAGCGACAGTGTGCGTCAGCGTGTCCTGGAGGCCGTTCAGCAGCTGAATTATGCCCCTTCTGCGCTGGCCCGCAGCT
TAAAGATGAACCAGACGCACACGCTCGGGATGTTGGTTACCACCAGTAATAATCCTTTTTTTGCTGAGGTGGTACGCGGG
GTAGAGCGCAGCTGCTATGAGCGGGGCTATAGCCTGATCCTGTGTAACACTGAGGGCGATCCGGCGCGTATGAGCCAGAG
TATGGAAACTCTGCTGCAAAAGCGGGTGGATGGTCTGCTGATGATGTGCACTGAGAATTTTCGGCCGGCGCAGGAGATGA
TTGCCCGTTATCCCGCGCTGCCGACGGTAATGATGGACTGGGCGCTGTTCGATGGCCAGAACGATATCATCCAGGATAAC
TCCTTCCTGGGGGGGCAAATAGCGACACGACATCTGATCGAACGCGGCTATACCCGCATAGCCTGTCTGGCGGGTCCTAA
GGATAAGTCCCCCTCTCGCCAGCGTCTGGAAGGCTTTCTGCACGAAATGAAACAGGCCGCGCTACCTGTGCCGCCGGAGT
ACATCCTGTTCGGCGATTTCGAGTTTGCCGGTGGAGTGCAGGCGATGAAACAGCTGCTGGCGCTGCCGACGCCGCCCCAT
GCGCTGTTTACCGGCAATGATGCGATGGCCGTCGGCGTATATCAGGTGCTGTTTGAAGCCGGACTGCGGGTGGGCGATGA
TGTCGCGGTCGTCGGCTATGACGATATTGAGCTGGCGCGCTATATGTCGCCGCCGTTGACGACGATTCATCAGCCCAAGG
ATGAGCTGGGAGAACTGGCCGTCGATACGCTGGTACACCGTCTGCAGCATCCCTCGCGGGAGCCACAGACACTGGTATTA
ACGCCCCAGCTTATTGAGCGGGGATCGGTCGCCATCCATCACGGTTGA

Upstream 100 bases:

>100_bases
TGCCGCGGCGATCGCCGTGACGCGTGAGGGCGCGCAGTCCTCTGTCCCTTGGCGCCGTGAGATCGATGCCTTCCTACAGA
CGCATCAGGAGTAATCATAC

Downstream 100 bases:

>100_bases
GGGGGAGTATCACGGTGATATGGGTGGCGGCGGCCCTTCAGCAAATGGTCGCCGTCCCTGGGATGGAGTAACATGAATAC
GTTGGCAGACAGCAGGGTGA

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATMKDVARLAGVSTSTVSHVINNNRFVSDSVRQRVLEAVQQLNYAPSALARSLKMNQTHTLGMLVTTSNNPFFAEVVRG
VERSCYERGYSLILCNTEGDPARMSQSMETLLQKRVDGLLMMCTENFRPAQEMIARYPALPTVMMDWALFDGQNDIIQDN
SFLGGQIATRHLIERGYTRIACLAGPKDKSPSRQRLEGFLHEMKQAALPVPPEYILFGDFEFAGGVQAMKQLLALPTPPH
ALFTGNDAMAVGVYQVLFEAGLRVGDDVAVVGYDDIELARYMSPPLTTIHQPKDELGELAVDTLVHRLQHPSREPQTLVL
TPQLIERGSVAIHHG

Sequences:

>Translated_335_residues
MATMKDVARLAGVSTSTVSHVINNNRFVSDSVRQRVLEAVQQLNYAPSALARSLKMNQTHTLGMLVTTSNNPFFAEVVRG
VERSCYERGYSLILCNTEGDPARMSQSMETLLQKRVDGLLMMCTENFRPAQEMIARYPALPTVMMDWALFDGQNDIIQDN
SFLGGQIATRHLIERGYTRIACLAGPKDKSPSRQRLEGFLHEMKQAALPVPPEYILFGDFEFAGGVQAMKQLLALPTPPH
ALFTGNDAMAVGVYQVLFEAGLRVGDDVAVVGYDDIELARYMSPPLTTIHQPKDELGELAVDTLVHRLQHPSREPQTLVL
TPQLIERGSVAIHHG
>Mature_334_residues
ATMKDVARLAGVSTSTVSHVINNNRFVSDSVRQRVLEAVQQLNYAPSALARSLKMNQTHTLGMLVTTSNNPFFAEVVRGV
ERSCYERGYSLILCNTEGDPARMSQSMETLLQKRVDGLLMMCTENFRPAQEMIARYPALPTVMMDWALFDGQNDIIQDNS
FLGGQIATRHLIERGYTRIACLAGPKDKSPSRQRLEGFLHEMKQAALPVPPEYILFGDFEFAGGVQAMKQLLALPTPPHA
LFTGNDAMAVGVYQVLFEAGLRVGDDVAVVGYDDIELARYMSPPLTTIHQPKDELGELAVDTLVHRLQHPSREPQTLVLT
PQLIERGSVAIHHG

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=66.8693009118541, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=46.3855421686747, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=34.5345345345345, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=32.5301204819277, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=28.5285285285285, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787580, Length=335, Percent_Identity=30.7462686567164, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=29.940119760479, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=33.2258064516129, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787906, Length=332, Percent_Identity=24.6987951807229, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=26.2839879154079, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789456, Length=344, Percent_Identity=28.1976744186047, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=25.0764525993884, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786268, Length=338, Percent_Identity=25.1479289940828, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790192, Length=254, Percent_Identity=28.740157480315, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=24.7678018575851, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37106; Mature: 36975

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINNNRFVSDSVRQRVLEAVQQLNYAPSALARSLKMNQTH
CCCHHHHHHHHCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TLGMLVTTSNNPFFAEVVRGVERSCYERGYSLILCNTEGDPARMSQSMETLLQKRVDGLL
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEE
MMCTENFRPAQEMIARYPALPTVMMDWALFDGQNDIIQDNSFLGGQIATRHLIERGYTRI
EEHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACLAGPKDKSPSRQRLEGFLHEMKQAALPVPPEYILFGDFEFAGGVQAMKQLLALPTPPH
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
ALFTGNDAMAVGVYQVLFEAGLRVGDDVAVVGYDDIELARYMSPPLTTIHQPKDELGELA
EEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHH
VDTLVHRLQHPSREPQTLVLTPQLIERGSVAIHHG
HHHHHHHHHCCCCCCCEEEECHHHHHCCCEEEECC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINNNRFVSDSVRQRVLEAVQQLNYAPSALARSLKMNQTH
CCHHHHHHHHCCCHHHHHHHHCCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TLGMLVTTSNNPFFAEVVRGVERSCYERGYSLILCNTEGDPARMSQSMETLLQKRVDGLL
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEE
MMCTENFRPAQEMIARYPALPTVMMDWALFDGQNDIIQDNSFLGGQIATRHLIERGYTRI
EEHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEE
ACLAGPKDKSPSRQRLEGFLHEMKQAALPVPPEYILFGDFEFAGGVQAMKQLLALPTPPH
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
ALFTGNDAMAVGVYQVLFEAGLRVGDDVAVVGYDDIELARYMSPPLTTIHQPKDELGELA
EEEECCCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHH
VDTLVHRLQHPSREPQTLVLTPQLIERGSVAIHHG
HHHHHHHHHCCCCCCCEEEECHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]