Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 238921714

GI number: 238921714

Start: 3756111

End: 3756734

Strand: Reverse

Name: dsbA [H]

Synonym: NT01EI_3876

Alternate gene names: 238921714

Gene position: 3756734-3756111 (Counterclockwise)

Preceding gene: 238921715

Following gene: 238921712

Centisome position: 98.54

GC content: 49.36

Gene sequence:

>624_bases
ATGAAAAAGATGTGGCTGGCCCTGCTGGGCATGGTTATAGCGTTTGGCGTATCGGCTGCGCAGTTTACTAACGGGGAACA
ATATACCACGTTGGATAAACCGGTAAACGGTGCGCCGCAGGTATTGGAATTCTTCTCGTTTTATTGTCCGCATTGCTATG
AGTTTGCTGAGGTTTACCATATTCCCCAGGCTATTGAAGGTAAACTGCCGCAGGGGATAACGCTGACAAAATACCATGTA
GAGTTTCTGGGGCCATTAGGAAAACAGCTGACTCAGGCATGGGCTGTCGCGATGGCGCTGGGAGTTGAGGATAAGATTAC
CCAGCCTATGTTTGATGCGCTACAGAAAACACAGAGTATTAAAACCGAGGCGGATATTCGCAATGTCTTTATTCAGGCCG
GAGTGAAACCGCAAGAGTATGATGCTGCGCTGAATAGCTTCGTGGTTAAATCTTTGGTGGTACAGCAGGAGAAAGCCGCA
GAGGATTTCCAACTGCGCGGTGTGCCGGCGGTATTTGTAAACGGCAGATACATGGTGAAAAGCGATGGATTGGATACCAG
CTCCATGGCGAACTATGTGGCGCAGTTTGCCAATGTGGTGCACTTCTTGGTGAGCCAGCCGTAA

Upstream 100 bases:

>100_bases
GATCCAGGTTTTTCAGGAGCAGGTCCGTCTGTTACAGGCCGCTCCGCTACAGTTAACGCCGATGTATTGACGACAGATCC
CGAATAATGGAGAAGTGATA

Downstream 100 bases:

>100_bases
TCGGCGCACTGACCCTGAGCAACACGCCGGATATCGTCTCTCCGGCGTTTTTTAGACTTTAGACGGTAGGACGGTAACGT
TCGCGTAGCCGTGTTATCAG

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKMWLALLGMVIAFGVSAAQFTNGEQYTTLDKPVNGAPQVLEFFSFYCPHCYEFAEVYHIPQAIEGKLPQGITLTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKITQPMFDALQKTQSIKTEADIRNVFIQAGVKPQEYDAALNSFVVKSLVVQQEKAA
EDFQLRGVPAVFVNGRYMVKSDGLDTSSMANYVAQFANVVHFLVSQP

Sequences:

>Translated_207_residues
MKKMWLALLGMVIAFGVSAAQFTNGEQYTTLDKPVNGAPQVLEFFSFYCPHCYEFAEVYHIPQAIEGKLPQGITLTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKITQPMFDALQKTQSIKTEADIRNVFIQAGVKPQEYDAALNSFVVKSLVVQQEKAA
EDFQLRGVPAVFVNGRYMVKSDGLDTSSMANYVAQFANVVHFLVSQP
>Mature_207_residues
MKKMWLALLGMVIAFGVSAAQFTNGEQYTTLDKPVNGAPQVLEFFSFYCPHCYEFAEVYHIPQAIEGKLPQGITLTKYHV
EFLGPLGKQLTQAWAVAMALGVEDKITQPMFDALQKTQSIKTEADIRNVFIQAGVKPQEYDAALNSFVVKSLVVQQEKAA
EDFQLRGVPAVFVNGRYMVKSDGLDTSSMANYVAQFANVVHFLVSQP

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=204, Percent_Identity=66.1764705882353, Blast_Score=293, Evalue=7e-81,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22964; Mature: 22964

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMWLALLGMVIAFGVSAAQFTNGEQYTTLDKPVNGAPQVLEFFSFYCPHCYEFAEVYH
CHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
IPQAIEGKLPQGITLTKYHVEFLGPLGKQLTQAWAVAMALGVEDKITQPMFDALQKTQSI
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KTEADIRNVFIQAGVKPQEYDAALNSFVVKSLVVQQEKAAEDFQLRGVPAVFVNGRYMVK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
SDGLDTSSMANYVAQFANVVHFLVSQP
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKMWLALLGMVIAFGVSAAQFTNGEQYTTLDKPVNGAPQVLEFFSFYCPHCYEFAEVYH
CHHHHHHHHHHHHHHCCHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
IPQAIEGKLPQGITLTKYHVEFLGPLGKQLTQAWAVAMALGVEDKITQPMFDALQKTQSI
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KTEADIRNVFIQAGVKPQEYDAALNSFVVKSLVVQQEKAAEDFQLRGVPAVFVNGRYMVK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEE
SDGLDTSSMANYVAQFANVVHFLVSQP
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10438793; 11586360; 12142430 [H]