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The map label for this gene is btuB [H]

Identifier: 238921670

GI number: 238921670

Start: 3710426

End: 3712273

Strand: Reverse

Name: btuB [H]

Synonym: NT01EI_3831

Alternate gene names: 238921670

Gene position: 3712273-3710426 (Counterclockwise)

Preceding gene: 238921671

Following gene: 238921669

Centisome position: 97.38

GC content: 57.95

Gene sequence:

>1848_bases
ATGAATAAGAAACATATGGTTATCGCGTTGTTTCCGGTGACGGCATCCGTGTCGGCCGGGGTGCAGGCGGCGCAGAGTGA
GATGGTGGTGACGGCCAGTCGCTTTGCCCAGCCGGTGTCGACGGTGCTGGCGCCGGTGGACATTGTGACCCGGGACGATA
TCGACCGCTGGCAGTCCAAGAGCCTAGACGAGGTCATGCGTCGCCTGCCGGGGATCGACATCGTCCAGAATGGTGGCTTG
GGGCAGAGCTCCTCGCTGTACCTGCGTGGTACAGAGGCGCGCCAAGCGCTGATTTTGGTCGACGGGATCCCTCTGGCTCG
CCCCGGCATCACCAATGTGGCTGCGCTGAATCAGATCCCGCTCGCCATGGTGCAGCGTATCGAATATATCCGCGGGCCGC
GTTCGGCGGTCTATGGCTCCGGAGCCATCGGTGGCGTTATCAATATCATTACGCTGGACGCCGAGCCCGGTGCGCAGCTG
AGCGTGGGCACCGGTACCAATCACTACCAGCAGTATGATGCCGGGGTGCGTCAGCAGCTGGGAAATACAACCCTATCGTT
GGCGGGCTCTTTTCAGACGACCCGCGGCTTTAATGTTCAGCCTGACTCGCCCTATGGGCCTGACGGCGATCGGGACGGGT
TTCGTAATAAAGTGCTGTGGGGTGGGGTAGAGCACCGTTTCTCTGAGGCCTGGTCTGGTTTTGTCCGCGGCCATGGCTAC
AGCGAAGCCTCTGATTATGATGCGGGGAAATACGGCAGCGATAGCAGCGGTACCCAGCGCCAGCTGTACAACCACAGCTA
CGATATGGGACTGCGCTATCATGCCGGCGCCTATGCGTCGCAGCTGATCGCCGCTTATCAAAAGATCAAGGATTACAACT
ATAGCGCAGTCTATGGCCGTTTTGGCAACGGCAGCTCCCTGGATGATCTACGGCAGTATAACCTGCAGTGGGGCAATACT
TACCGCTTTGACAGCGGCATGCTCAGTGCCGGGGCCGACTGGCAGCAGCAACGTTTGATCTCATCAGATACCAAGAGGAC
GCAACATTACCAGCGCTACGACGCTGGATTTTATCTGAGTGGTCAACACAAAGTGGGCAGCGTGACACTCGAGGCCTCTG
GACGGGCGGACAATGATCAGCAATTTGGCTGGCATGGTACCTGGCAGAGCGCCGCTGGCTGGGAGTTTATCCCCGGCTAC
CGTGCGACGCTCTCTTACGGCACCGGTTTTCTGGCACCGTCGCTGGGGCAGCAATTTGGCTCCGATCGTATGGATATACA
AAGCAATCCCAACCTGCGCCCGGAGGAGTCGCGCCAGTGGGAGGTGGGGCTGAGCGGCCTGAGCGGTCCGCTGGACTGGC
AGCTGTCGGCCTACCGTAATCAGGTTCGCAACCTGATCACCTACTTTAGCGATCCGATCACCCGACACGGACAGTACAGT
AATATTGACTCGGCGACCATCAAAGGCGTCGAGTGGGTCGGCAGTATGGATACCTGGGCGCTGAGCCACCGCGTGACCCT
GCAATATATTGATCCCCGAGATGATAAAACCGGAGACCTGCTGCCGCGTCGCTCCCGTCATCAGGGTAAGTACCAGTTGG
ATTGGAACCTTTGGGATCTCGGGATGGATCTGGCCTACCAGTATTTCGGCCGCAGTAGCGATAAAAGCGCGCCGGGCGGT
AAGCTGCCCAGCTACAGCACGGTAGACATTTCGGCCTCTTATCCGGTTACATCCCAGCTCACGGTTCGTGGTAGAATTGG
TAATCTGTTAGATAAAAATTATCAAACCGCTTATGGCTACCAAACCGCAGGACGGGAATATTATCTCTCCGGTAGCTACA
CCTTCTGA

Upstream 100 bases:

>100_bases
GGTATCCCTATGCCGATGTGCTGGGTAGAATGCCCTGTTTTTTGAGTGAGGCAGCAGGGAGCCGGCCTGTTATCTTGGGT
GTAGAGAGAAGGCAATTATA

Downstream 100 bases:

>100_bases
TGATGTAGCCACTGCCAAGCCTACCGTGCTGGTGTTTGACTCCGGTGTCGGTGGGCTTTCTGTCTATCAGGAGATCCGCG
CGTTACTACCGGATCTCCAC

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MNKKHMVIALFPVTASVSAGVQAAQSEMVVTASRFAQPVSTVLAPVDIVTRDDIDRWQSKSLDEVMRRLPGIDIVQNGGL
GQSSSLYLRGTEARQALILVDGIPLARPGITNVAALNQIPLAMVQRIEYIRGPRSAVYGSGAIGGVINIITLDAEPGAQL
SVGTGTNHYQQYDAGVRQQLGNTTLSLAGSFQTTRGFNVQPDSPYGPDGDRDGFRNKVLWGGVEHRFSEAWSGFVRGHGY
SEASDYDAGKYGSDSSGTQRQLYNHSYDMGLRYHAGAYASQLIAAYQKIKDYNYSAVYGRFGNGSSLDDLRQYNLQWGNT
YRFDSGMLSAGADWQQQRLISSDTKRTQHYQRYDAGFYLSGQHKVGSVTLEASGRADNDQQFGWHGTWQSAAGWEFIPGY
RATLSYGTGFLAPSLGQQFGSDRMDIQSNPNLRPEESRQWEVGLSGLSGPLDWQLSAYRNQVRNLITYFSDPITRHGQYS
NIDSATIKGVEWVGSMDTWALSHRVTLQYIDPRDDKTGDLLPRRSRHQGKYQLDWNLWDLGMDLAYQYFGRSSDKSAPGG
KLPSYSTVDISASYPVTSQLTVRGRIGNLLDKNYQTAYGYQTAGREYYLSGSYTF

Sequences:

>Translated_615_residues
MNKKHMVIALFPVTASVSAGVQAAQSEMVVTASRFAQPVSTVLAPVDIVTRDDIDRWQSKSLDEVMRRLPGIDIVQNGGL
GQSSSLYLRGTEARQALILVDGIPLARPGITNVAALNQIPLAMVQRIEYIRGPRSAVYGSGAIGGVINIITLDAEPGAQL
SVGTGTNHYQQYDAGVRQQLGNTTLSLAGSFQTTRGFNVQPDSPYGPDGDRDGFRNKVLWGGVEHRFSEAWSGFVRGHGY
SEASDYDAGKYGSDSSGTQRQLYNHSYDMGLRYHAGAYASQLIAAYQKIKDYNYSAVYGRFGNGSSLDDLRQYNLQWGNT
YRFDSGMLSAGADWQQQRLISSDTKRTQHYQRYDAGFYLSGQHKVGSVTLEASGRADNDQQFGWHGTWQSAAGWEFIPGY
RATLSYGTGFLAPSLGQQFGSDRMDIQSNPNLRPEESRQWEVGLSGLSGPLDWQLSAYRNQVRNLITYFSDPITRHGQYS
NIDSATIKGVEWVGSMDTWALSHRVTLQYIDPRDDKTGDLLPRRSRHQGKYQLDWNLWDLGMDLAYQYFGRSSDKSAPGG
KLPSYSTVDISASYPVTSQLTVRGRIGNLLDKNYQTAYGYQTAGREYYLSGSYTF
>Mature_615_residues
MNKKHMVIALFPVTASVSAGVQAAQSEMVVTASRFAQPVSTVLAPVDIVTRDDIDRWQSKSLDEVMRRLPGIDIVQNGGL
GQSSSLYLRGTEARQALILVDGIPLARPGITNVAALNQIPLAMVQRIEYIRGPRSAVYGSGAIGGVINIITLDAEPGAQL
SVGTGTNHYQQYDAGVRQQLGNTTLSLAGSFQTTRGFNVQPDSPYGPDGDRDGFRNKVLWGGVEHRFSEAWSGFVRGHGY
SEASDYDAGKYGSDSSGTQRQLYNHSYDMGLRYHAGAYASQLIAAYQKIKDYNYSAVYGRFGNGSSLDDLRQYNLQWGNT
YRFDSGMLSAGADWQQQRLISSDTKRTQHYQRYDAGFYLSGQHKVGSVTLEASGRADNDQQFGWHGTWQSAAGWEFIPGY
RATLSYGTGFLAPSLGQQFGSDRMDIQSNPNLRPEESRQWEVGLSGLSGPLDWQLSAYRNQVRNLITYFSDPITRHGQYS
NIDSATIKGVEWVGSMDTWALSHRVTLQYIDPRDDKTGDLLPRRSRHQGKYQLDWNLWDLGMDLAYQYFGRSSDKSAPGG
KLPSYSTVDISASYPVTSQLTVRGRIGNLLDKNYQTAYGYQTAGREYYLSGSYTF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=626, Percent_Identity=48.4025559105431, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1788478, Length=658, Percent_Identity=25.3799392097264, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786798, Length=760, Percent_Identity=23.8157894736842, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68120; Mature: 68120

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKHMVIALFPVTASVSAGVQAAQSEMVVTASRFAQPVSTVLAPVDIVTRDDIDRWQSK
CCCCEEEEEEEECHHCHHHHHHHHHHHHEEEHHHHHHHHHHHHHCHHEEECCCHHHHHCC
SLDEVMRRLPGIDIVQNGGLGQSSSLYLRGTEARQALILVDGIPLARPGITNVAALNQIP
CHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHH
LAMVQRIEYIRGPRSAVYGSGAIGGVINIITLDAEPGAQLSVGTGTNHYQQYDAGVRQQL
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
GNTTLSLAGSFQTTRGFNVQPDSPYGPDGDRDGFRNKVLWGGVEHRFSEAWSGFVRGHGY
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
SEASDYDAGKYGSDSSGTQRQLYNHSYDMGLRYHAGAYASQLIAAYQKIKDYNYSAVYGR
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
FGNGSSLDDLRQYNLQWGNTYRFDSGMLSAGADWQQQRLISSDTKRTQHYQRYDAGFYLS
CCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
GQHKVGSVTLEASGRADNDQQFGWHGTWQSAAGWEFIPGYRATLSYGTGFLAPSLGQQFG
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHC
SDRMDIQSNPNLRPEESRQWEVGLSGLSGPLDWQLSAYRNQVRNLITYFSDPITRHGQYS
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCC
NIDSATIKGVEWVGSMDTWALSHRVTLQYIDPRDDKTGDLLPRRSRHQGKYQLDWNLWDL
CCCHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHH
GMDLAYQYFGRSSDKSAPGGKLPSYSTVDISASYPVTSQLTVRGRIGNLLDKNYQTAYGY
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHCCCCCEECCE
QTAGREYYLSGSYTF
ECCCCEEEECCCCCC
>Mature Secondary Structure
MNKKHMVIALFPVTASVSAGVQAAQSEMVVTASRFAQPVSTVLAPVDIVTRDDIDRWQSK
CCCCEEEEEEEECHHCHHHHHHHHHHHHEEEHHHHHHHHHHHHHCHHEEECCCHHHHHCC
SLDEVMRRLPGIDIVQNGGLGQSSSLYLRGTEARQALILVDGIPLARPGITNVAALNQIP
CHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHH
LAMVQRIEYIRGPRSAVYGSGAIGGVINIITLDAEPGAQLSVGTGTNHYQQYDAGVRQQL
HHHHHHHHHHCCCCHHEECCCCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHH
GNTTLSLAGSFQTTRGFNVQPDSPYGPDGDRDGFRNKVLWGGVEHRFSEAWSGFVRGHGY
CCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
SEASDYDAGKYGSDSSGTQRQLYNHSYDMGLRYHAGAYASQLIAAYQKIKDYNYSAVYGR
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEEEEE
FGNGSSLDDLRQYNLQWGNTYRFDSGMLSAGADWQQQRLISSDTKRTQHYQRYDAGFYLS
CCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEE
GQHKVGSVTLEASGRADNDQQFGWHGTWQSAAGWEFIPGYRATLSYGTGFLAPSLGQQFG
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCCCCCCHHHHHHC
SDRMDIQSNPNLRPEESRQWEVGLSGLSGPLDWQLSAYRNQVRNLITYFSDPITRHGQYS
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCC
NIDSATIKGVEWVGSMDTWALSHRVTLQYIDPRDDKTGDLLPRRSRHQGKYQLDWNLWDL
CCCHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHH
GMDLAYQYFGRSSDKSAPGGKLPSYSTVDISASYPVTSQLTVRGRIGNLLDKNYQTAYGY
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCHHHHHHCCCCCEECCE
QTAGREYYLSGSYTF
ECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA