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The map label for this gene is emrD [H]

Identifier: 238921635

GI number: 238921635

Start: 3670300

End: 3671484

Strand: Direct

Name: emrD [H]

Synonym: NT01EI_3790

Alternate gene names: 238921635

Gene position: 3670300-3671484 (Clockwise)

Preceding gene: 238921632

Following gene: 238921639

Centisome position: 96.27

GC content: 59.58

Gene sequence:

>1185_bases
ATGAGAAAGTTAGAAAATTTTCACCTGCTGGTCATGTTGATCCTGTTGGTCGCTGTCGGTCAGATGGCACAGACGATTTA
TGTTCCCTCTATCCCGGATATGGGACGCGATCTGGGCGTGCGCAGCGGCGCGATACAAAGCGTGATGGCGGCGTATTTGC
TGACCTATGGCGCCTCGCAGCTAATCTATGGTCCGCTCTCCGATCGCATCGGCCGTCGGCCGGTGATCCTGGCAGGAATG
GGCATTTTTATGCTGGGTGCATTGCAGGCTATGCTGGCGCCCAGCCTGCATATGCTGGTGATGGCGGCAGCGCTACAGGG
GATGGGTACCGGTGTCGCCGGGGTGATAGCCCGTACCATGCCGCGCGATCTGTACAACGGTACCTCGCTGCGTTATGCCA
ATAGCCTGTTGAATATGGGGATTCTGGTCAGCCCTCTGCTGGCGCCGGTGATTGGTGGCCTATTGGACGCCTTTATCAGC
TGGCGCGCCTGCTATGCATTTCTGCTGCTATTATGCGCCGGAGTCGCCTACAGCATGTTCCGCTGGCTGCCGGAGACCCG
ACCGGCCGCGGCGGGTCACAGCAACCTGTTCAGCAGCTACCGTCAGCTGCTGAGTTGTGGCAGCTTTGTCTGCTATCTGG
TGATGCTGATCGGGGGACTGGCGGGGGTCGCGGTGTTTGAGGCCTGCTCCGGCGTTCTATTGGGCGGCGTGCTGCACTTA
AGCGGTATCGTGGTCAGCATTCTGTTTATTCTACCGATCCCGGCGGCGTTCTTTGGTGCATGGTATGCCGGGCGTCCGGG
CAAGCCCTTTGCCACCCTGATGTGGCATGCGGTGCTGAGCTGCCTGCTGGCTGGGTTGCTGATGTGGATGCCGAGCTGGT
TTGGCATCATCAATATCTGGACGCTGATCGTACCGGCGGCACTGTTCTTTTTTGGTGCCGGCATGCTGTTTCCTCTGGCG
ACGACCGGTGCCATGGAGCCGTTTCCACTGTTGGCCGGGGCCGCCGGCGCGCTGGTGGGCGGACTGCAGAACGTCGGATC
TGGCGCGATGGCCTGGTTTTCCGCCCTGCTGCCGCAGAACGGACAGTTTAGCCTGGGGATGCTGATGAGTGCGATGGCGG
TAATGATTTTGCTGTGCTGGCTGCCGATTGCACACAAGGTGCGTCAGCAAGGACAGATCATGTAA

Upstream 100 bases:

>100_bases
TCTGTTAGATTGTGCGCAACAATATAGTTATTGATTTTACATATATGGCTATATGGTGGTTTATAGTCCTGGTTAATAAT
GCGTTGGTAAACGTAACATA

Downstream 100 bases:

>100_bases
TACCCAGGATGTCGGGCTGATTCACAGCGGCGTGCCGAATGGCACGCCGTTTTACGTTGGTCACCCGCACTGTTACAGAA
TGATATCGCGGATCTGAGCG

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MRKLENFHLLVMLILLVAVGQMAQTIYVPSIPDMGRDLGVRSGAIQSVMAAYLLTYGASQLIYGPLSDRIGRRPVILAGM
GIFMLGALQAMLAPSLHMLVMAAALQGMGTGVAGVIARTMPRDLYNGTSLRYANSLLNMGILVSPLLAPVIGGLLDAFIS
WRACYAFLLLLCAGVAYSMFRWLPETRPAAAGHSNLFSSYRQLLSCGSFVCYLVMLIGGLAGVAVFEACSGVLLGGVLHL
SGIVVSILFILPIPAAFFGAWYAGRPGKPFATLMWHAVLSCLLAGLLMWMPSWFGIINIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPLLAGAAGALVGGLQNVGSGAMAWFSALLPQNGQFSLGMLMSAMAVMILLCWLPIAHKVRQQGQIM

Sequences:

>Translated_394_residues
MRKLENFHLLVMLILLVAVGQMAQTIYVPSIPDMGRDLGVRSGAIQSVMAAYLLTYGASQLIYGPLSDRIGRRPVILAGM
GIFMLGALQAMLAPSLHMLVMAAALQGMGTGVAGVIARTMPRDLYNGTSLRYANSLLNMGILVSPLLAPVIGGLLDAFIS
WRACYAFLLLLCAGVAYSMFRWLPETRPAAAGHSNLFSSYRQLLSCGSFVCYLVMLIGGLAGVAVFEACSGVLLGGVLHL
SGIVVSILFILPIPAAFFGAWYAGRPGKPFATLMWHAVLSCLLAGLLMWMPSWFGIINIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPLLAGAAGALVGGLQNVGSGAMAWFSALLPQNGQFSLGMLMSAMAVMILLCWLPIAHKVRQQGQIM
>Mature_394_residues
MRKLENFHLLVMLILLVAVGQMAQTIYVPSIPDMGRDLGVRSGAIQSVMAAYLLTYGASQLIYGPLSDRIGRRPVILAGM
GIFMLGALQAMLAPSLHMLVMAAALQGMGTGVAGVIARTMPRDLYNGTSLRYANSLLNMGILVSPLLAPVIGGLLDAFIS
WRACYAFLLLLCAGVAYSMFRWLPETRPAAAGHSNLFSSYRQLLSCGSFVCYLVMLIGGLAGVAVFEACSGVLLGGVLHL
SGIVVSILFILPIPAAFFGAWYAGRPGKPFATLMWHAVLSCLLAGLLMWMPSWFGIINIWTLIVPAALFFFGAGMLFPLA
TTGAMEPFPLLAGAAGALVGGLQNVGSGAMAWFSALLPQNGQFSLGMLMSAMAVMILLCWLPIAHKVRQQGQIM

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=392, Percent_Identity=70.4081632653061, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI48994889, Length=350, Percent_Identity=26.5714285714286, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788509, Length=357, Percent_Identity=24.0896358543417, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790146, Length=351, Percent_Identity=27.3504273504274, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790794, Length=323, Percent_Identity=25.3869969040248, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787065, Length=170, Percent_Identity=28.2352941176471, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=176, Percent_Identity=28.4090909090909, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41883; Mature: 41883

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]