Click here to switch to the map view.

The map label for this gene is bcsZ [H]

Identifier: 238921604

GI number: 238921604

Start: 3643276

End: 3644397

Strand: Reverse

Name: bcsZ [H]

Synonym: NT01EI_3757

Alternate gene names: 238921604

Gene position: 3644397-3643276 (Counterclockwise)

Preceding gene: 238921605

Following gene: 238921603

Centisome position: 95.6

GC content: 58.47

Gene sequence:

>1122_bases
ATGAAAGAGTCTGTTTACCGTACACTGATGGCCGCTGCGTTGCTGTGTGTCAGCGTCGCCACCGTTGGCGCACCCTGTGA
ATGGCATGAGTGGGAGCAGTTTCGTCAGGGCTATATCAGCCCTGACGGGCGGGTTATCGATCCCAGTTCTCCCCGCCAGG
TAACCACCTCGGAGGGGCAAAGTTATGCGCTGTTCTTTGCCTTGGTCAATAACGATCGCGCGTTGTTCGGTAAGTTATTG
GCATGGACGCAGAATAATCTGTCGCAGGGCAGTCTGAGCCAGCGTTTGCCAGCCTGGAAGTGGGGACGCCATGATGACGG
GCGTTGGCGCATATTGGATGATAACTCTGCCTCGGATGCCGACCTGTGGATAGCCTATACGCTGCTGGAGGCCGGGCGCC
TGTGGCAGGATTCCGATTACCAACATTTGAGTATTCAGCTATTGGCGCGGATTGCCCGGGAGGAGGTGGTCTCCTTGCCG
GGATTTGGCATGATGCTGCTGCCGGGTCGTCAGGGGTTCCGCTCTGGCGCTCTGTGGACGATAAATCCAAGCTACCTGCC
ACCGCAGATCCTGGCTCGGATTGCGCCATTGCATGGACCGTGGAACCGTCTGCAGTACAGCGCATTGCGCATGTTAGAGG
CGTCGTCGCCGCATGGCATCGTGCCGGACTGGACAAACTGGCAGCGGAATAAGGGCTGGCTGCCGACGCCGGGGCGGGCC
TATATCAGCGGCTATGATGCCATCCGGGCCTATCTGTGGGCGGGAATGCTGGCCGATGGCGCTCCGGCCAAAAGCGCGCT
GGTGAAGCACTGGCAGCCGATGGTGGCGTTGAGTGAGCGTCTGGGATATATCCCTGAAAAGGTCGGTGTACTGGGCGTAG
AGCCCCAGGGTGAAGGCAACGTTGGGTTCTCGGCCGCCATGTTGCCGTTTCTGGATGCCAGCAACAGTCCGTTATTGGCG
GCGCAGCGGCAGCGGGTAAAAGACAACTTGCCGGGGAATAATAACTACTACAGTTCAGTCCTGACGCTGTTTGGCCTGGG
ATGGGATCAGCGGCGTTACCGTTTTAATCTACAGGGTGAACTGGTGCCAAACTGGGGTTCCGTATGCGATGTTTCCGACT
GA

Upstream 100 bases:

>100_bases
CATTGGCGCTGTTGGCGATTATTCTGGTGAGCCTGGTGCTGTGGCAGGTGCCGTGGATGCTGCGTCGCCGTCTGGATCCC
GACGATAGGAAAATAAGCAT

Downstream 100 bases:

>100_bases
GCCTGACTGCCTTACCGGTGACACTGGCGTTGAGTACGCCAGTGAGGTGCGGGTATGCATAGGCGAATCCGCGCAGCGTT
ATGATCTGGTAACGCAGTTG

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKESVYRTLMAAALLCVSVATVGAPCEWHEWEQFRQGYISPDGRVIDPSSPRQVTTSEGQSYALFFALVNNDRALFGKLL
AWTQNNLSQGSLSQRLPAWKWGRHDDGRWRILDDNSASDADLWIAYTLLEAGRLWQDSDYQHLSIQLLARIAREEVVSLP
GFGMMLLPGRQGFRSGALWTINPSYLPPQILARIAPLHGPWNRLQYSALRMLEASSPHGIVPDWTNWQRNKGWLPTPGRA
YISGYDAIRAYLWAGMLADGAPAKSALVKHWQPMVALSERLGYIPEKVGVLGVEPQGEGNVGFSAAMLPFLDASNSPLLA
AQRQRVKDNLPGNNNYYSSVLTLFGLGWDQRRYRFNLQGELVPNWGSVCDVSD

Sequences:

>Translated_373_residues
MKESVYRTLMAAALLCVSVATVGAPCEWHEWEQFRQGYISPDGRVIDPSSPRQVTTSEGQSYALFFALVNNDRALFGKLL
AWTQNNLSQGSLSQRLPAWKWGRHDDGRWRILDDNSASDADLWIAYTLLEAGRLWQDSDYQHLSIQLLARIAREEVVSLP
GFGMMLLPGRQGFRSGALWTINPSYLPPQILARIAPLHGPWNRLQYSALRMLEASSPHGIVPDWTNWQRNKGWLPTPGRA
YISGYDAIRAYLWAGMLADGAPAKSALVKHWQPMVALSERLGYIPEKVGVLGVEPQGEGNVGFSAAMLPFLDASNSPLLA
AQRQRVKDNLPGNNNYYSSVLTLFGLGWDQRRYRFNLQGELVPNWGSVCDVSD
>Mature_373_residues
MKESVYRTLMAAALLCVSVATVGAPCEWHEWEQFRQGYISPDGRVIDPSSPRQVTTSEGQSYALFFALVNNDRALFGKLL
AWTQNNLSQGSLSQRLPAWKWGRHDDGRWRILDDNSASDADLWIAYTLLEAGRLWQDSDYQHLSIQLLARIAREEVVSLP
GFGMMLLPGRQGFRSGALWTINPSYLPPQILARIAPLHGPWNRLQYSALRMLEASSPHGIVPDWTNWQRNKGWLPTPGRA
YISGYDAIRAYLWAGMLADGAPAKSALVKHWQPMVALSERLGYIPEKVGVLGVEPQGEGNVGFSAAMLPFLDASNSPLLA
AQRQRVKDNLPGNNNYYSSVLTLFGLGWDQRRYRFNLQGELVPNWGSVCDVSD

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=357, Percent_Identity=52.1008403361345, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41731; Mature: 41731

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESVYRTLMAAALLCVSVATVGAPCEWHEWEQFRQGYISPDGRVIDPSSPRQVTTSEGQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCC
SYALFFALVNNDRALFGKLLAWTQNNLSQGSLSQRLPAWKWGRHDDGRWRILDDNSASDA
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCC
DLWIAYTLLEAGRLWQDSDYQHLSIQLLARIAREEVVSLPGFGMMLLPGRQGFRSGALWT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEE
INPSYLPPQILARIAPLHGPWNRLQYSALRMLEASSPHGIVPDWTNWQRNKGWLPTPGRA
ECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
YISGYDAIRAYLWAGMLADGAPAKSALVKHWQPMVALSERLGYIPEKVGVLGVEPQGEGN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCEEECCCCCCCC
VGFSAAMLPFLDASNSPLLAAQRQRVKDNLPGNNNYYSSVLTLFGLGWDQRRYRFNLQGE
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCE
LVPNWGSVCDVSD
ECCCCCCCCCCCC
>Mature Secondary Structure
MKESVYRTLMAAALLCVSVATVGAPCEWHEWEQFRQGYISPDGRVIDPSSPRQVTTSEGQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCEEECCCCC
SYALFFALVNNDRALFGKLLAWTQNNLSQGSLSQRLPAWKWGRHDDGRWRILDDNSASDA
EEEEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCC
DLWIAYTLLEAGRLWQDSDYQHLSIQLLARIAREEVVSLPGFGMMLLPGRQGFRSGALWT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEE
INPSYLPPQILARIAPLHGPWNRLQYSALRMLEASSPHGIVPDWTNWQRNKGWLPTPGRA
ECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
YISGYDAIRAYLWAGMLADGAPAKSALVKHWQPMVALSERLGYIPEKVGVLGVEPQGEGN
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCEEECCCCCCCC
VGFSAAMLPFLDASNSPLLAAQRQRVKDNLPGNNNYYSSVLTLFGLGWDQRRYRFNLQGE
CCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCE
LVPNWGSVCDVSD
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]