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The map label for this gene is pitA [H]

Identifier: 238921571

GI number: 238921571

Start: 3611691

End: 3613145

Strand: Direct

Name: pitA [H]

Synonym: NT01EI_3724

Alternate gene names: 238921571

Gene position: 3611691-3613145 (Clockwise)

Preceding gene: 238921570

Following gene: 238921574

Centisome position: 94.74

GC content: 59.45

Gene sequence:

>1455_bases
ATGTTAGTCCTCGCGCTGCTCTTTGTGCTGTTCTACGAAGCCATCAACGGCTTCCACGATACGGCCAATGCGGTCGCAAC
CGTGATTTACACCCGTGCGCTGCGTTCGCAGATCGCCGTGGTAATGTCCGGTATTTTTAACTTCCTCGGCGTCATGCTGG
GCGGCCTGAGCGTGGCCTATGCCATCGTTCACCTGCTGCCGACCGACCTGCTGCTGAACGTGGGCTCCGCCCATGGGCTG
GCCATGATGTTCTCCCTGCTGCTGGCGGCGATCATCTGGAACCTGGGAACCTGGTATTTCGGTCTGCCGGCCTCCAGCTC
CCACACCCTGATCGGGGCCATCATCGGTATCGCGCTGACCAACGCGCTCATGACCCATACCTCCGTGATGGATGCGCTTA
ACATACCGAAGATGATTGAGATCTTCCTCTCACTGATCATCTCGCCGATCGTCGGCCTGCTGCTCGCCGGTATGATAGTC
TGGATGCTACGCCGCTACTGGAGTGGAACCAAAAAACGTCGCCGTATTCATATGACTCCGGCCGAGCGTGAAAAGATCGA
CGGCAAACGTAAGCCACCGTTCTGGACCCGTATCGCCCTGATCATATCGGCTATCGGTGTCAGCTTTTCCCATGGCGCGA
ACGATGGGCAGAAAGGGATCGGCCTGCTGATGCTGGTACTGATCGGTGTCGCCCCGGCAGGTTTCGTGATGAACATGGAC
GCGACGGGATACGATATTGCCCGTACCCGCGATGCCATTACCCACCTGGATCAATACTATCGTCGGCATCAGGCTGAGCT
GAGCCACGTCATCAGCGCGGAACCGGAAGTCCCGGCCAACGAAGATGTCAAAGGCGCCAAGATATTCCACTGCGACCCTG
CCCGTGCCCAGGTCGCCATCGCCAGTGCGCAGGGCATGCTGACCGATCTGCAAAGCTACGATCAGCTGAGCGTCGATCAG
CGCAGCCATATGCGCCGCCTGCTGATGTGCGTCGCCGATACGGCAGGTAAAGTGGCCAAACTGCCGGAAACCAGCGAAAC
CAATAAGCGCTTCCTGACCGATCTGCGTAAAGATCTGCTGGCGACAGTGGAATATGCTCCGATGTGGATCATCGTCGCCG
TTGCCCTGGCTCTGTCACTGGGCACCATGGTCGGCTGGCGCCGCGTCGCCACCACCATCGGCGAAAAGATCGGGAAGAAA
GGGATGACCTACGCGCAGGGGGTCTCCGCGCAAATGACCGCCGCGGTCTCCATCGGCGTGGCCAGCTACACCGGGATGCC
GGTCTCCACCACCCATGTACTCTCCTCTGCAGTCACCGGCACCATGCTGGTCGACGGCGGCGGCGTACAGGGCAAAACGG
TGAAGAACATCCTGCTGGCCTGGGTACTGACGTTGCCCATGGCAATCATCATCTCCAGCCTGCTGTACTGGCTGGCGCTC
ACCTTCGTGGTCTAA

Upstream 100 bases:

>100_bases
ACCCAGCCTTCTCGCCGATAATGCGCCGCGTTCATGTCCACAAACTGGCGTAACGCATATGCTACATCTCTTTGCAGGGC
TAGATTTCCATACTGGCCTA

Downstream 100 bases:

>100_bases
CGGTCCGTTAAACGATAAAAAAGCCCGCAAATGCGGGCTTTTTTTTGGTAATTGACCTGTCCTGAATAGCGTTGACACGT
TGCTGACTAAAATCTGGAGA

Product: low-affinity inorganic phosphate transporter 2

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MLVLALLFVLFYEAINGFHDTANAVATVIYTRALRSQIAVVMSGIFNFLGVMLGGLSVAYAIVHLLPTDLLLNVGSAHGL
AMMFSLLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIALTNALMTHTSVMDALNIPKMIEIFLSLIISPIVGLLLAGMIV
WMLRRYWSGTKKRRRIHMTPAEREKIDGKRKPPFWTRIALIISAIGVSFSHGANDGQKGIGLLMLVLIGVAPAGFVMNMD
ATGYDIARTRDAITHLDQYYRRHQAELSHVISAEPEVPANEDVKGAKIFHCDPARAQVAIASAQGMLTDLQSYDQLSVDQ
RSHMRRLLMCVADTAGKVAKLPETSETNKRFLTDLRKDLLATVEYAPMWIIVAVALALSLGTMVGWRRVATTIGEKIGKK
GMTYAQGVSAQMTAAVSIGVASYTGMPVSTTHVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPMAIIISSLLYWLAL
TFVV

Sequences:

>Translated_484_residues
MLVLALLFVLFYEAINGFHDTANAVATVIYTRALRSQIAVVMSGIFNFLGVMLGGLSVAYAIVHLLPTDLLLNVGSAHGL
AMMFSLLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIALTNALMTHTSVMDALNIPKMIEIFLSLIISPIVGLLLAGMIV
WMLRRYWSGTKKRRRIHMTPAEREKIDGKRKPPFWTRIALIISAIGVSFSHGANDGQKGIGLLMLVLIGVAPAGFVMNMD
ATGYDIARTRDAITHLDQYYRRHQAELSHVISAEPEVPANEDVKGAKIFHCDPARAQVAIASAQGMLTDLQSYDQLSVDQ
RSHMRRLLMCVADTAGKVAKLPETSETNKRFLTDLRKDLLATVEYAPMWIIVAVALALSLGTMVGWRRVATTIGEKIGKK
GMTYAQGVSAQMTAAVSIGVASYTGMPVSTTHVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPMAIIISSLLYWLAL
TFVV
>Mature_484_residues
MLVLALLFVLFYEAINGFHDTANAVATVIYTRALRSQIAVVMSGIFNFLGVMLGGLSVAYAIVHLLPTDLLLNVGSAHGL
AMMFSLLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIALTNALMTHTSVMDALNIPKMIEIFLSLIISPIVGLLLAGMIV
WMLRRYWSGTKKRRRIHMTPAEREKIDGKRKPPFWTRIALIISAIGVSFSHGANDGQKGIGLLMLVLIGVAPAGFVMNMD
ATGYDIARTRDAITHLDQYYRRHQAELSHVISAEPEVPANEDVKGAKIFHCDPARAQVAIASAQGMLTDLQSYDQLSVDQ
RSHMRRLLMCVADTAGKVAKLPETSETNKRFLTDLRKDLLATVEYAPMWIIVAVALALSLGTMVGWRRVATTIGEKIGKK
GMTYAQGVSAQMTAAVSIGVASYTGMPVSTTHVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPMAIIISSLLYWLAL
TFVV

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=475, Percent_Identity=73.0526315789474, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=474, Percent_Identity=68.1434599156118, Blast_Score=658, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146401, Length=516, Percent_Identity=22.4806201550388, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52394; Mature: 52394

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVLALLFVLFYEAINGFHDTANAVATVIYTRALRSQIAVVMSGIFNFLGVMLGGLSVAY
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVHLLPTDLLLNVGSAHGLAMMFSLLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIALT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHH
NALMTHTSVMDALNIPKMIEIFLSLIISPIVGLLLAGMIVWMLRRYWSGTKKRRRIHMTP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCC
AEREKIDGKRKPPFWTRIALIISAIGVSFSHGANDGQKGIGLLMLVLIGVAPAGFVMNMD
HHHHHCCCCCCCCHHHHHHHHHHHHHCHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
ATGYDIARTRDAITHLDQYYRRHQAELSHVISAEPEVPANEDVKGAKIFHCDPARAQVAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHEEH
ASAQGMLTDLQSYDQLSVDQRSHMRRLLMCVADTAGKVAKLPETSETNKRFLTDLRKDLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHH
ATVEYAPMWIIVAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGVSAQMTAAVSIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHH
ASYTGMPVSTTHVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPMAIIISSLLYWLAL
HHHCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVV
HHHC
>Mature Secondary Structure
MLVLALLFVLFYEAINGFHDTANAVATVIYTRALRSQIAVVMSGIFNFLGVMLGGLSVAY
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVHLLPTDLLLNVGSAHGLAMMFSLLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIALT
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHH
NALMTHTSVMDALNIPKMIEIFLSLIISPIVGLLLAGMIVWMLRRYWSGTKKRRRIHMTP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEECCC
AEREKIDGKRKPPFWTRIALIISAIGVSFSHGANDGQKGIGLLMLVLIGVAPAGFVMNMD
HHHHHCCCCCCCCHHHHHHHHHHHHHCHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
ATGYDIARTRDAITHLDQYYRRHQAELSHVISAEPEVPANEDVKGAKIFHCDPARAQVAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHEEH
ASAQGMLTDLQSYDQLSVDQRSHMRRLLMCVADTAGKVAKLPETSETNKRFLTDLRKDLL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHH
ATVEYAPMWIIVAVALALSLGTMVGWRRVATTIGEKIGKKGMTYAQGVSAQMTAAVSIGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHH
ASYTGMPVSTTHVLSSAVTGTMLVDGGGVQGKTVKNILLAWVLTLPMAIIISSLLYWLAL
HHHCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVV
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]