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The map label for this gene is rsmD [H]

Identifier: 238921554

GI number: 238921554

Start: 3594156

End: 3594767

Strand: Direct

Name: rsmD [H]

Synonym: NT01EI_3706

Alternate gene names: 238921554

Gene position: 3594156-3594767 (Clockwise)

Preceding gene: 238921545

Following gene: 238921555

Centisome position: 94.28

GC content: 63.4

Gene sequence:

>612_bases
ATGGCAAAAAAACCTCACGCACAATCCTGCGGCCAGATCCGCATCATCGCCGGACAGTGGCGCGGCCGCAAACTGCCGGT
GCCGGACAGCCCGGGCCTCCGCCCCACCACCGACCGGGTACGTGAAACCCTGTTCAACTGGCTGGCACCGATCCTGCACG
ATACCCAGTGTCTGGATGCCTTCGCCGGCAGCGGCGCCTTGGGGTTTGAAGCCGTCTCACGCTATGCAGCACGCGCGACC
CTGCTGGAGGCCGATCGCCTCGTGGCAGCGGGGCTGCGCAAAAACGCGGCCCTGCTCAACGCCCCCCAGCTTGAGGTGAT
CCATACCGATACACTGCGCTTCCTGGCGCAAAGGGGAACACCGTATCAGGTGGTATTTCTCGATCCGCCCTTTCGCAAGG
GGCTGCTGAACGACACGCTGACACTGCTGGAGCAGAACGGCTGGCTGGCGGCGGACGCCTGGATTTATGTCGAGTGTGAG
GCTGACAGCCCTGCCCTGGCCACTCCGGCCAGCTGGGCGTTACAGCGTGAAAAGGTCGCCGGACAGGTCGCCTACCGGCT
ATACCGGCGCCAGGTAACAGAGCAAAACGAGGGAGACAACCATGTGGATTAA

Upstream 100 bases:

>100_bases
GCTCGCATCCACTTTGCCGCCGACTCCGATAGCGGGCGAACGTAGGACAGACATCCGTCTGACTCCACTCACTGTTTCCC
CAACGACACTAACAGATTAC

Downstream 100 bases:

>100_bases
TATCGGCCGCCTGCTGATGCTGGGTGTATGGGGATTCATGCTGGCCAACCTGCTGCACGCATTTCCGCGCCCGCTGAACA
TCTTCGTCAACGTAGCGATG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MAKKPHAQSCGQIRIIAGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPILHDTQCLDAFAGSGALGFEAVSRYAARAT
LLEADRLVAAGLRKNAALLNAPQLEVIHTDTLRFLAQRGTPYQVVFLDPPFRKGLLNDTLTLLEQNGWLAADAWIYVECE
ADSPALATPASWALQREKVAGQVAYRLYRRQVTEQNEGDNHVD

Sequences:

>Translated_203_residues
MAKKPHAQSCGQIRIIAGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPILHDTQCLDAFAGSGALGFEAVSRYAARAT
LLEADRLVAAGLRKNAALLNAPQLEVIHTDTLRFLAQRGTPYQVVFLDPPFRKGLLNDTLTLLEQNGWLAADAWIYVECE
ADSPALATPASWALQREKVAGQVAYRLYRRQVTEQNEGDNHVD
>Mature_202_residues
AKKPHAQSCGQIRIIAGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPILHDTQCLDAFAGSGALGFEAVSRYAARATL
LEADRLVAAGLRKNAALLNAPQLEVIHTDTLRFLAQRGTPYQVVFLDPPFRKGLLNDTLTLLEQNGWLAADAWIYVECEA
DSPALATPASWALQREKVAGQVAYRLYRRQVTEQNEGDNHVD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=197, Percent_Identity=64.4670050761421, Blast_Score=270, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22514; Mature: 22383

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPHAQSCGQIRIIAGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPILHDTQCLDA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FAGSGALGFEAVSRYAARATLLEADRLVAAGLRKNAALLNAPQLEVIHTDTLRFLAQRGT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEHHHHHHHHHCCC
PYQVVFLDPPFRKGLLNDTLTLLEQNGWLAADAWIYVECEADSPALATPASWALQREKVA
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEECEEEEEEECCCCCCCCCCHHHHHHHHHHH
GQVAYRLYRRQVTEQNEGDNHVD
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AKKPHAQSCGQIRIIAGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPILHDTQCLDA
CCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FAGSGALGFEAVSRYAARATLLEADRLVAAGLRKNAALLNAPQLEVIHTDTLRFLAQRGT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEHHHHHHHHHCCC
PYQVVFLDPPFRKGLLNDTLTLLEQNGWLAADAWIYVECEADSPALATPASWALQREKVA
CEEEEEECCCHHHHHHHHHHHHHHCCCCEEECEEEEEEECCCCCCCCCCHHHHHHHHHHH
GQVAYRLYRRQVTEQNEGDNHVD
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]