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The map label for this gene is dusB [H]

Identifier: 238921361

GI number: 238921361

Start: 3401698

End: 3402702

Strand: Direct

Name: dusB [H]

Synonym: NT01EI_3505

Alternate gene names: 238921361

Gene position: 3401698-3402702 (Clockwise)

Preceding gene: 238921360

Following gene: 238921362

Centisome position: 89.23

GC content: 56.82

Gene sequence:

>1005_bases
ATGCGCATCGGAAATATCCAACTTAAAAATCGTCTGATCGCCGCGCCCATGGCGGGCATTACCGATCGACCCTTTCGCAC
CCTGTGCTATGAGATGGGCGTCGGTATGGCGGTTTCCGAGATGCTCTCTTCCAACCCGGAAGTATGGCGGACGGATAAGT
CACGTCTGCGCATGGTACATATGGATGAGCCAGGTATCCGAGCCGTGCAGATCGCCGGTAACGACCCCGATGAGATGGCC
GCGGCAGCCCGCATCAATGCTGATAACGGCGCGCAGATCATCGACATCAATATGGGTTGTCCGGCAAAGAAAGTGAATCG
CAAGCTGGCCGGTTCTGCCCTGCTGCAGTATCCCGGACTGGTGGAACAGATCCTGCGTGCCGTCGTGGACGCGGTGGATG
TTCCGGTGACACTCAAGATACGCACCGGCTGGGATCCTGCGCACCGTAACTGTGTCGAGATTGCCCAATTGGCTGAACGC
TGTGGCATTCAGGCCCTGACCATTCATGGCCGAACCCGTGCCTGCCTGTTTAACGGCGAGGCCGAGTACGACAGCATTCG
GACAGTTAAGCAGCATGTTTCCATTCCGGTCATCGCGAACGGAGACATAACTGACCCGCATAAAGCCAGGGCGGTGCTCG
ACTACACCGGAGCCGATGCTCTGATGATTGGTCGCGCCGCTCAGGGAAGACCCTGGATCTTCCGGGAAATCCAGCATTAT
CTGGACACAGGGGAGCTGCTGCCGCCCATGCCATTGGGCGAGATAAAGCGGCTATTGGCCGGGCATGTCAAAGAGCTGCA
TGATTTTTACGGCTCGGGAAAAGGACTGCGCATCGCCCGTAAACACGTGTCCTGGTATCTCCAGGCGCACGCCCCGAATG
ACCAGTTTCGGCGCACATTCAACGCCATTGAGGACGCCAGCGAGCAGCTGGAGGCGTTGGAGGCATACTTCGAAAAACTT
AGCGTAAAAAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAA

Upstream 100 bases:

>100_bases
GATCTGCAACAGTCGATTGTTCAAAGTTTGGCCTTTCATATCTGTGCAAAAATGCGTAATATACGCGCCCTTGCGGACAC
AGTATGGTCACTCTTTGTCT

Downstream 100 bases:

>100_bases
ATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGACCAGGTTACTCAAAAACCTCTGCGTGACTCGGTCAAACAG
GCACTGAAGAACTATTTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRIGNIQLKNRLIAAPMAGITDRPFRTLCYEMGVGMAVSEMLSSNPEVWRTDKSRLRMVHMDEPGIRAVQIAGNDPDEMA
AAARINADNGAQIIDINMGCPAKKVNRKLAGSALLQYPGLVEQILRAVVDAVDVPVTLKIRTGWDPAHRNCVEIAQLAER
CGIQALTIHGRTRACLFNGEAEYDSIRTVKQHVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEIKRLLAGHVKELHDFYGSGKGLRIARKHVSWYLQAHAPNDQFRRTFNAIEDASEQLEALEAYFEKL
SVKKELTELCSNNA

Sequences:

>Translated_334_residues
MRIGNIQLKNRLIAAPMAGITDRPFRTLCYEMGVGMAVSEMLSSNPEVWRTDKSRLRMVHMDEPGIRAVQIAGNDPDEMA
AAARINADNGAQIIDINMGCPAKKVNRKLAGSALLQYPGLVEQILRAVVDAVDVPVTLKIRTGWDPAHRNCVEIAQLAER
CGIQALTIHGRTRACLFNGEAEYDSIRTVKQHVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEIKRLLAGHVKELHDFYGSGKGLRIARKHVSWYLQAHAPNDQFRRTFNAIEDASEQLEALEAYFEKL
SVKKELTELCSNNA
>Mature_334_residues
MRIGNIQLKNRLIAAPMAGITDRPFRTLCYEMGVGMAVSEMLSSNPEVWRTDKSRLRMVHMDEPGIRAVQIAGNDPDEMA
AAARINADNGAQIIDINMGCPAKKVNRKLAGSALLQYPGLVEQILRAVVDAVDVPVTLKIRTGWDPAHRNCVEIAQLAER
CGIQALTIHGRTRACLFNGEAEYDSIRTVKQHVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEIKRLLAGHVKELHDFYGSGKGLRIARKHVSWYLQAHAPNDQFRRTFNAIEDASEQLEALEAYFEKL
SVKKELTELCSNNA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=245, Percent_Identity=31.4285714285714, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=31.5352697095436, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=34.3612334801762, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=30.6772908366534, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=30.4347826086957, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=85.981308411215, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1788462, Length=269, Percent_Identity=30.1115241635688, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693211, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=245, Percent_Identity=30.2040816326531, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=31.4285714285714, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=33.1838565022422, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=268, Percent_Identity=28.7313432835821, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=30.2631578947368, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=29.2887029288703, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580595, Length=252, Percent_Identity=31.7460317460317, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920448, Length=252, Percent_Identity=31.7460317460317, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45549423, Length=258, Percent_Identity=29.8449612403101, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.82096069869, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37052; Mature: 37052

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGNIQLKNRLIAAPMAGITDRPFRTLCYEMGVGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCCEECCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHEEEEE
MDEPGIRAVQIAGNDPDEMAAAARINADNGAQIIDINMGCPAKKVNRKLAGSALLQYPGL
ECCCCCEEEEECCCCHHHHHHHHEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
VEQILRAVVDAVDVPVTLKIRTGWDPAHRNCVEIAQLAERCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRTVKQHVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPMPLGEIKRLLAGHVKELHDFYGSGKGLRIARKHVSWYLQAHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCHHHHHHH
NAIEDASEQLEALEAYFEKLSVKKELTELCSNNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIGNIQLKNRLIAAPMAGITDRPFRTLCYEMGVGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCCEECCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHEEEEE
MDEPGIRAVQIAGNDPDEMAAAARINADNGAQIIDINMGCPAKKVNRKLAGSALLQYPGL
ECCCCCEEEEECCCCHHHHHHHHEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
VEQILRAVVDAVDVPVTLKIRTGWDPAHRNCVEIAQLAERCGIQALTIHGRTRACLFNGE
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRTVKQHVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPMPLGEIKRLLAGHVKELHDFYGSGKGLRIARKHVSWYLQAHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCCHHHHHHH
NAIEDASEQLEALEAYFEKLSVKKELTELCSNNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9811652 [H]