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The map label for this gene is amiD [H]

Identifier: 238921292

GI number: 238921292

Start: 3324139

End: 3324909

Strand: Direct

Name: amiD [H]

Synonym: NT01EI_3435

Alternate gene names: 238921292

Gene position: 3324139-3324909 (Clockwise)

Preceding gene: 238921291

Following gene: 238921293

Centisome position: 87.19

GC content: 44.88

Gene sequence:

>771_bases
ATGTATTCCATTGATTATAATAGCTATCGATCGGTGAAAAGTTTCAACCGCCGTGTTCGCTTCCTGGTTATGCATTACAC
CGCTATTAATTTTAAGGCGTCAGTTGCCGCGTTGACCGGATCTTCCGTCAGCGCCCACTATCTTATTCCTGACCCTTCAG
AAAAAACGTATATCGATGCCGGTTTTAACGATATGCGTATTTTCAATCTGGTTGATGAAAATGAGCGGTCCTGGCATGCA
GGTAGCAGCTCATGGGCTGGACGTACTAATCTGAATGACACGGCCATCGGTATTGAGATAGTTAATCTGGCGACTGACAA
TAATAACGTATTTGTATTCCCGCCGTATAACCCAACACAAATTGCAGCAATTAAAGAGCTGGCCGCCAATATTCTGCAGA
GATATCCCGATATTACGCCTACGAATATTGTCGGGCACAGCGATATTGCCCCAGGGAGAAAAAGCGATCCCGGGGCGGCT
TTCCCGTGGAAAGCGCTTTATGAAGCAGGCATCGGCGCATGGTATGAAGAGTCGACCATGCAGCACTATCTGGCGCAGTT
CAGCAAATCGCTGCCAGCTAAAGCAGATATTATTGCCAAGTTAAAAACATACGGATATGATACTTCAGATGCAGGAAAAG
AAAGTGGATACAGAAATTTAATCCGTGCATTTCAGCTTCACTTCCGACAAAAAAATTATGATGGTGGCGCTGATGCTGAA
ACAACGGCAATTCTCTATGCGCTGGTAGAAAAATATTTCCCGGAAAAGTAA

Upstream 100 bases:

>100_bases
ACAAAAATGCCCCCGGGATCACGTCTATATCAACCTTGTATACATTTTGTTGTCATACATACTGGTATTACTGGCTTATA
CTATAAAAAAGGAGTTTGCT

Downstream 100 bases:

>100_bases
TTACATCCCAAAAATCTGAGCGCCCTGCATGTGGATAAATCTTATCATTTATTCACATGTGGTTTGACTTATACAGCGCG
CCTGAGAGAGCATCCTGAGC

Product: N-acetylmuramoyl-L-alanine amidase AmiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MYSIDYNSYRSVKSFNRRVRFLVMHYTAINFKASVAALTGSSVSAHYLIPDPSEKTYIDAGFNDMRIFNLVDENERSWHA
GSSSWAGRTNLNDTAIGIEIVNLATDNNNVFVFPPYNPTQIAAIKELAANILQRYPDITPTNIVGHSDIAPGRKSDPGAA
FPWKALYEAGIGAWYEESTMQHYLAQFSKSLPAKADIIAKLKTYGYDTSDAGKESGYRNLIRAFQLHFRQKNYDGGADAE
TTAILYALVEKYFPEK

Sequences:

>Translated_256_residues
MYSIDYNSYRSVKSFNRRVRFLVMHYTAINFKASVAALTGSSVSAHYLIPDPSEKTYIDAGFNDMRIFNLVDENERSWHA
GSSSWAGRTNLNDTAIGIEIVNLATDNNNVFVFPPYNPTQIAAIKELAANILQRYPDITPTNIVGHSDIAPGRKSDPGAA
FPWKALYEAGIGAWYEESTMQHYLAQFSKSLPAKADIIAKLKTYGYDTSDAGKESGYRNLIRAFQLHFRQKNYDGGADAE
TTAILYALVEKYFPEK
>Mature_256_residues
MYSIDYNSYRSVKSFNRRVRFLVMHYTAINFKASVAALTGSSVSAHYLIPDPSEKTYIDAGFNDMRIFNLVDENERSWHA
GSSSWAGRTNLNDTAIGIEIVNLATDNNNVFVFPPYNPTQIAAIKELAANILQRYPDITPTNIVGHSDIAPGRKSDPGAA
FPWKALYEAGIGAWYEESTMQHYLAQFSKSLPAKADIIAKLKTYGYDTSDAGKESGYRNLIRAFQLHFRQKNYDGGADAE
TTAILYALVEKYFPEK

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=254, Percent_Identity=44.0944881889764, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI1786300, Length=133, Percent_Identity=36.8421052631579, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28665; Mature: 28665

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSIDYNSYRSVKSFNRRVRFLVMHYTAINFKASVAALTGSSVSAHYLIPDPSEKTYIDA
CCCCCCHHHHHHHHHHHHHEEEEEEEEEECCEEEEEEEECCCCEEEEECCCCCCCCEEEC
GFNDMRIFNLVDENERSWHAGSSSWAGRTNLNDTAIGIEIVNLATDNNNVFVFPPYNPTQ
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCHH
IAAIKELAANILQRYPDITPTNIVGHSDIAPGRKSDPGAAFPWKALYEAGIGAWYEESTM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHH
QHYLAQFSKSLPAKADIIAKLKTYGYDTSDAGKESGYRNLIRAFQLHFRQKNYDGGADAE
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TTAILYALVEKYFPEK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYSIDYNSYRSVKSFNRRVRFLVMHYTAINFKASVAALTGSSVSAHYLIPDPSEKTYIDA
CCCCCCHHHHHHHHHHHHHEEEEEEEEEECCEEEEEEEECCCCEEEEECCCCCCCCEEEC
GFNDMRIFNLVDENERSWHAGSSSWAGRTNLNDTAIGIEIVNLATDNNNVFVFPPYNPTQ
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEECCCCCHH
IAAIKELAANILQRYPDITPTNIVGHSDIAPGRKSDPGAAFPWKALYEAGIGAWYEESTM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHH
QHYLAQFSKSLPAKADIIAKLKTYGYDTSDAGKESGYRNLIRAFQLHFRQKNYDGGADAE
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TTAILYALVEKYFPEK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]