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The map label for this gene is trmD

Identifier: 238921086

GI number: 238921086

Start: 3098420

End: 3099184

Strand: Reverse

Name: trmD

Synonym: NT01EI_3221

Alternate gene names: 238921086

Gene position: 3099184-3098420 (Counterclockwise)

Preceding gene: 238921087

Following gene: 238921085

Centisome position: 81.29

GC content: 57.52

Gene sequence:

>765_bases
ATGTGGATTGGGGTAATTAGTCTGTTTCCCGAGATGTTCCGCGCAATCACCGATTACGGGGTAACTGGCCGGGCCGTAAA
AAAAGGCCTGCTTAGCGTGCAGTGCTGGAATCCGCGCGATTTCACGCACGATCGGCATCGAACCGTGGACGATCGCCCCT
ATGGCGGCGGGCCGGGGATGCTGATGATGGTTCAACCCCTGCGGGATGCCATCCATGCGGCAAAGGCAGCGGCAGGCGAA
GGGGCGAAGGTGATTTATCTTTCGCCTCAGGGGCGCAAACTGGACCAGCAGGGCGTTTGCGAACTCTCCGTCAGCCAGAA
GTTAATTCTGGTCTGCGGGCGTTATGAGGGTATTGATGAGCGCGTAATCCAAACCGAAATCGATGAAGAGTGGTCGATCG
GCGATTACGTTCTCAGCGGCGGCGAACTGCCGGCCATGACTCTGATTGACTCGATCTCCCGGTTTGTGCCGGGGGTGTTG
GGTGATCAGGCCTCGGCTGAGGAGGATTCGTTTGTCGACGGTCTGCTGGACTGTCCACACTATACCCGTCCTGAGGTGTT
ACAGGATATGGAGGTACCGCCAGTGCTGCTGTCGGGAAACCATGCCGAGATACGTCGCTGGCGCTTAAAGCAGTCGCTGG
GCCGAACCTGGCTTAGAAGACCTGAACTTCTAGAAAGCCTAGCTCTGACTGACGAGCAAGCAAAGCTGCTGACGCAGTTC
CGCAAGGAGCATGCCGCGCAGCAGGATCATGAAGGCTGCGACTAA

Upstream 100 bases:

>100_bases
TGATCAAGAAAGTCGATCTCACTGCTAAATCTATTGAAGTAGATTGGGATCCTGGTTTTTGACCTCCGATATTAACGGTA
GAACTGAGTGGAACGCCACG

Downstream 100 bases:

>100_bases
AACGTCGGGCCGATATATCAGTTTACCTAGGGTAAGAGATTATTATGAACAATATCATTAAACAGATTGAACAAGAGCAG
ATGAAGCAAGACGTACCTGC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MWIGVISLFPEMFRAITDYGVTGRAVKKGLLSVQCWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVCELSVSQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSISRFVPGVL
GDQASAEEDSFVDGLLDCPHYTRPEVLQDMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLESLALTDEQAKLLTQF
RKEHAAQQDHEGCD

Sequences:

>Translated_254_residues
MWIGVISLFPEMFRAITDYGVTGRAVKKGLLSVQCWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVCELSVSQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSISRFVPGVL
GDQASAEEDSFVDGLLDCPHYTRPEVLQDMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLESLALTDEQAKLLTQF
RKEHAAQQDHEGCD
>Mature_254_residues
MWIGVISLFPEMFRAITDYGVTGRAVKKGLLSVQCWNPRDFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVCELSVSQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSISRFVPGVL
GDQASAEEDSFVDGLLDCPHYTRPEVLQDMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLESLALTDEQAKLLTQF
RKEHAAQQDHEGCD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=253, Percent_Identity=88.1422924901186, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_EDWI9 (C5BGG0)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934601.1
- GeneID:   7961058
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3221
- OMA:   VCGRFEG
- ProtClustDB:   CLSK2813582
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28426; Mature: 28426

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIGVISLFPEMFRAITDYGVTGRAVKKGLLSVQCWNPRDFTHDRHRTVDDRPYGGGPGM
CEEHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCCCCHHCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQQGVCELSVSQKLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCCCHH
RVIQTEIDEEWSIGDYVLSGGELPAMTLIDSISRFVPGVLGDQASAEEDSFVDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
YTRPEVLQDMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLESLALTDEQAKLLTQF
CCCHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
RKEHAAQQDHEGCD
HHHHHHHCCCCCCC
>Mature Secondary Structure
MWIGVISLFPEMFRAITDYGVTGRAVKKGLLSVQCWNPRDFTHDRHRTVDDRPYGGGPGM
CEEHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCCCCCCCHHCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQQGVCELSVSQKLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCCCHH
RVIQTEIDEEWSIGDYVLSGGELPAMTLIDSISRFVPGVLGDQASAEEDSFVDGLLDCPH
HHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
YTRPEVLQDMEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLESLALTDEQAKLLTQF
CCCHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHH
RKEHAAQQDHEGCD
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA