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The map label for this gene is clpB [H]

Identifier: 238921070

GI number: 238921070

Start: 3083146

End: 3085719

Strand: Reverse

Name: clpB [H]

Synonym: NT01EI_3205

Alternate gene names: 238921070

Gene position: 3085719-3083146 (Counterclockwise)

Preceding gene: 238921071

Following gene: 238921069

Centisome position: 80.94

GC content: 56.18

Gene sequence:

>2574_bases
ATGCGTCTGGATCGTTTAACCAACAAGTTCCAGTTAGCCCTCGCTGATGCCCAGTCTTTGGCACTCGGGCGCGACAACCA
ATTTATTGAACCGATACATCTGATGAGCGCGATGCTCGGTCAGGATGGGGGCACGGTTCGTCCTTTGCTGGTATCTGCAG
GCGTTGATATCGGTGAGTTTCAGCAGTCCCTGGAGCAGGCTATTGCCCGCCTACCGCAGGTGGAGGGGACCGGTGGCGAT
GTTCAGCCATCCAACGACCTGATTCGGGTTCTCAACCTGTGTGATAAATTAGCGCAAAAGCACAGTGATACCTACATATC
GTCAGAGCTGTTTATACTGGCCGCGATGGAAGAGCGCGGTAAACTGGCCGATATGTTGAAGGCCGCTGGTGCTTCTGCGC
AAAAACTCAGCGTTGCTGTTGAGCAGCTGCGCGGAGGAGAAAAAGTGGACGATCAGAATGCGGAAGATCAGCGCCAGGCA
CTGAATAAGTACACCATCGATCTGACCGAGCGTGCCGAGCTGGGTAAGCTGGATCCGGTGATTGGGCGTGACGAAGAGAT
CCGCCGCACCATTCAGGTGCTGCAGCGCCGCACCAAAAATAATCCGGTGCTGATCGGGGAGCCCGGGGTCGGTAAAACGG
CTATTGTCGAAGGGCTGGCCCAGCGCATCATCAACGGTGAGGTCCCTGAGGGGCTGAAGCACAAGCGCGTGCTTTCTTTG
GATATGGGGGCGTTAATCGCCGGTGCAAAATTCCGCGGTGAGTTTGAAGAACGTTTGAAAGCGGTGCTGAACGATCTGGC
TAAACAGGAGGGCAGTGTCATCCTGTTTATCGATGAGCTGCACACGATGGTCGGGGCTGGAAAAGCCGATGGCGCCATGG
ATGCGGGGAATATGCTGAAGCCTGCGCTGGCGCGTGGTGAGCTGCATTGTGTCGGTGCGACGACCCTGGACGAGTACCGC
CAGTATATTGAGAAGGATGCTGCACTGGAGCGTCGTTTCCAGAAGGTCTATGTGGCGCAGCCGTCCGTCGAAGATACCAT
CGCCATTCTTCGTGGGTTGAAGGAGCGCTATGAGCTACATCACCACGTGCAGATCACCGATCCAGCTATCGTGGCGGCCG
CCACGCTATCCCACCGCTATATCGCTGATCGCCAGCTGCCCGATAAGGCCATCGACCTGATCGATGAGGCCGCTTCCAGC
ATCCGGATGCAGATCGACTCTAAACCGGAAGCGTTGGATCGTCTGGAACGGCGTATCATCCAGCTTAAGCTGGAGCAGCA
GGCGCTGAAGAAAGAGTCCGACGAGGCCAGCAAGAAACGTCTGGAAATCCTGAATATTGAGCTGGAGGAGAAAGAGCGCG
ACTATGCCTCGCTGGAAGAAGAGTGGAAGGCAGAAAAAGCCGCGCTGACCGGTACCCAGAATATCAAGGCCGAGATCGAG
CAGGCCAAGATTGCGCTAGAGCAGGCGCGCCGCAGCGGTGACCTTGCGCAGATGTCTGAGATCCAGTACGGCAAACTGCC
GGATTTGGAGAAGCAGCTGCAGGCGGCAACGCAGGCCGAAGGTAAGAGTATGAAGCTGCTGCGTAATAAGGTCACCGACG
CCGAAATCGCAGAGGTGCTGGCGCGTTGGACCGGTATTCCAGTATCGCGCATGCTGGAAGGTGAGCGCGAAAAATTGCTG
CGTATGGAAGGTGAGCTGCATAAACGCGTCGTGGGGCAGAATGAGGCGGTTGAAGCTGTCTCCAATGCTATTCGCCGTAG
CCGCGCAGGGCTGTCCGATCCTAACCGTCCCATCGGTTCATTCCTGTTCCTCGGGCCGACTGGGGTGGGTAAAACCGAAC
TGTGTAAGGCGCTGGCTAACTTCTTGTTCGACAGCGATGACGCTATGGTGCGTATCGACATGTCTGAATTTATGGAGAAG
CACTCCGTATCGCGTTTGGTCGGGGCGCCTCCGGGATATGTCGGCTATGAAGAGGGGGGATATTTGACGGAGGCAGTACG
CCGTCGTCCGTACTCGGTCATCTTGCTCGATGAGGTCGAGAAGGCGCATCCAGATGTCTTTAACATCCTGCTGCAGGTGC
TGGACGATGGACGTCTGACTGATGGTCAGGGGCGCACCGTCGATTTTCGTAATACGGTGGTCATTATGACGTCTAATCTG
GGCTCCGATCTGATCCAGGATCGCTTTGGCGACCAGAGCTATGGCGAAATGAAAGCGCTGGTGATGAGCGTAGTCACTCA
CCATTTCAGACCAGAGTTCATCAATCGTATTGATGAGACAGTGGTCTTCCATCCGTTGGGTCAGGAGAACATTAAGGCGA
TCGCCCGCATTCAGTTGCAGCGGCTGTATCAGCGGTTGGAAGAGAAGGGCTACAGCGTCACGATGAGCGAGGCGGCGTTG
GATCAGCTCTCTCAGGCTGGCTTCGACCCGGTTTATGGTGCACGTCCGTTGAAGCGTGCCATCCAACAGGAGATCGAAAA
CCCGTTGGCACAACAGCTCCTGTCCGGGAAACTGTTGCCGGGCAAACCGATTACCCTGGTGTTGGACGGCGAGCAGATCG
TGGCACAGCAGTAA

Upstream 100 bases:

>100_bases
GAACAAAAAATAATTAAATAATTGTCACGATTTGGTTGAAAAGTGCAGTCTAGACCACACTTATTCTCCAGAAGCAATTT
GCATACGTCTCGGAGGAAAC

Downstream 100 bases:

>100_bases
TTCACGATAGCGCAATACGGGGGCAGCCTGATGGCTGCCCTGTCTTTTACCTCTTCTGATGGGGAGGAGGCTGGAAAAGG
TATGAAAATAACGCGCTCTG

Product: protein disaggregation chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRLTNKFQLALADAQSLALGRDNQFIEPIHLMSAMLGQDGGTVRPLLVSAGVDIGEFQQSLEQAIARLPQVEGTGGD
VQPSNDLIRVLNLCDKLAQKHSDTYISSELFILAAMEERGKLADMLKAAGASAQKLSVAVEQLRGGEKVDDQNAEDQRQA
LNKYTIDLTERAELGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DMGALIAGAKFRGEFEERLKAVLNDLAKQEGSVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAQPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRMQIDSKPEALDRLERRIIQLKLEQQALKKESDEASKKRLEILNIELEEKERDYASLEEEWKAEKAALTGTQNIKAEIE
QAKIALEQARRSGDLAQMSEIQYGKLPDLEKQLQAATQAEGKSMKLLRNKVTDAEIAEVLARWTGIPVSRMLEGEREKLL
RMEGELHKRVVGQNEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALANFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQDRFGDQSYGEMKALVMSVVTHHFRPEFINRIDETVVFHPLGQENIKAIARIQLQRLYQRLEEKGYSVTMSEAAL
DQLSQAGFDPVYGARPLKRAIQQEIENPLAQQLLSGKLLPGKPITLVLDGEQIVAQQ

Sequences:

>Translated_857_residues
MRLDRLTNKFQLALADAQSLALGRDNQFIEPIHLMSAMLGQDGGTVRPLLVSAGVDIGEFQQSLEQAIARLPQVEGTGGD
VQPSNDLIRVLNLCDKLAQKHSDTYISSELFILAAMEERGKLADMLKAAGASAQKLSVAVEQLRGGEKVDDQNAEDQRQA
LNKYTIDLTERAELGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DMGALIAGAKFRGEFEERLKAVLNDLAKQEGSVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAQPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRMQIDSKPEALDRLERRIIQLKLEQQALKKESDEASKKRLEILNIELEEKERDYASLEEEWKAEKAALTGTQNIKAEIE
QAKIALEQARRSGDLAQMSEIQYGKLPDLEKQLQAATQAEGKSMKLLRNKVTDAEIAEVLARWTGIPVSRMLEGEREKLL
RMEGELHKRVVGQNEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALANFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQDRFGDQSYGEMKALVMSVVTHHFRPEFINRIDETVVFHPLGQENIKAIARIQLQRLYQRLEEKGYSVTMSEAAL
DQLSQAGFDPVYGARPLKRAIQQEIENPLAQQLLSGKLLPGKPITLVLDGEQIVAQQ
>Mature_857_residues
MRLDRLTNKFQLALADAQSLALGRDNQFIEPIHLMSAMLGQDGGTVRPLLVSAGVDIGEFQQSLEQAIARLPQVEGTGGD
VQPSNDLIRVLNLCDKLAQKHSDTYISSELFILAAMEERGKLADMLKAAGASAQKLSVAVEQLRGGEKVDDQNAEDQRQA
LNKYTIDLTERAELGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
DMGALIAGAKFRGEFEERLKAVLNDLAKQEGSVILFIDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYR
QYIEKDAALERRFQKVYVAQPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRMQIDSKPEALDRLERRIIQLKLEQQALKKESDEASKKRLEILNIELEEKERDYASLEEEWKAEKAALTGTQNIKAEIE
QAKIALEQARRSGDLAQMSEIQYGKLPDLEKQLQAATQAEGKSMKLLRNKVTDAEIAEVLARWTGIPVSRMLEGEREKLL
RMEGELHKRVVGQNEAVEAVSNAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTELCKALANFLFDSDDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQDRFGDQSYGEMKALVMSVVTHHFRPEFINRIDETVVFHPLGQENIKAIARIQLQRLYQRLEEKGYSVTMSEAAL
DQLSQAGFDPVYGARPLKRAIQQEIENPLAQQLLSGKLLPGKPITLVLDGEQIVAQQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=308, Percent_Identity=36.038961038961, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=84.4807467911318, Blast_Score=1494, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=325, Percent_Identity=43.6923076923077, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=52.3943661971831, Blast_Score=758, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=43.2748538011696, Blast_Score=686, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95202; Mature: 95202

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRLTNKFQLALADAQSLALGRDNQFIEPIHLMSAMLGQDGGTVRPLLVSAGVDIGEF
CCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHH
QQSLEQAIARLPQVEGTGGDVQPSNDLIRVLNLCDKLAQKHSDTYISSELFILAAMEERG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCC
KLADMLKAAGASAQKLSVAVEQLRGGEKVDDQNAEDQRQALNKYTIDLTERAELGKLDPV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECHHCCCCCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEHEE
DMGALIAGAKFRGEFEERLKAVLNDLAKQEGSVILFIDELHTMVGAGKADGAMDAGNMLK
CHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAQPSVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKPEALDRLERRII
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKLEQQALKKESDEASKKRLEILNIELEEKERDYASLEEEWKAEKAALTGTQNIKAEIE
HHHHHHHHHHCCHHHHHHHHHHHEEEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QAKIALEQARRSGDLAQMSEIQYGKLPDLEKQLQAATQAEGKSMKLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
ARWTGIPVSRMLEGEREKLLRMEGELHKRVVGQNEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALANFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQDRFGDQSYGEMKALVMSVVTHHFRPEFINRIDETVVFHPLGQENIKAIARIQLQ
CHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RLYQRLEEKGYSVTMSEAALDQLSQAGFDPVYGARPLKRAIQQEIENPLAQQLLSGKLLP
HHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GKPITLVLDGEQIVAQQ
CCCEEEEECCHHHHCCC
>Mature Secondary Structure
MRLDRLTNKFQLALADAQSLALGRDNQFIEPIHLMSAMLGQDGGTVRPLLVSAGVDIGEF
CCCHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHH
QQSLEQAIARLPQVEGTGGDVQPSNDLIRVLNLCDKLAQKHSDTYISSELFILAAMEERG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCC
KLADMLKAAGASAQKLSVAVEQLRGGEKVDDQNAEDQRQALNKYTIDLTERAELGKLDPV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECHHCCCCCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIINGEVPEGLKHKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEHEE
DMGALIAGAKFRGEFEERLKAVLNDLAKQEGSVILFIDELHTMVGAGKADGAMDAGNMLK
CHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYIEKDAALERRFQKVYVAQPSVEDTIAILRGLKERYELH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVQITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRMQIDSKPEALDRLERRII
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKLEQQALKKESDEASKKRLEILNIELEEKERDYASLEEEWKAEKAALTGTQNIKAEIE
HHHHHHHHHHCCHHHHHHHHHHHEEEEEHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QAKIALEQARRSGDLAQMSEIQYGKLPDLEKQLQAATQAEGKSMKLLRNKVTDAEIAEVL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHH
ARWTGIPVSRMLEGEREKLLRMEGELHKRVVGQNEAVEAVSNAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALANFLFDSDDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQDRFGDQSYGEMKALVMSVVTHHFRPEFINRIDETVVFHPLGQENIKAIARIQLQ
CHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RLYQRLEEKGYSVTMSEAALDQLSQAGFDPVYGARPLKRAIQQEIENPLAQQLLSGKLLP
HHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GKPITLVLDGEQIVAQQ
CCCEEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]