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The map label for this gene is braB [H]

Identifier: 238920912

GI number: 238920912

Start: 2928202

End: 2929572

Strand: Reverse

Name: braB [H]

Synonym: NT01EI_3040

Alternate gene names: 238920912

Gene position: 2929572-2928202 (Counterclockwise)

Preceding gene: 238920913

Following gene: 238920911

Centisome position: 76.84

GC content: 63.09

Gene sequence:

>1371_bases
ATGCAACAACGTTTATCGATGGCTGACACTCTGGGGCTGGGATTAATGACCTTTGCCTTTTTTCTGGGCGCCGGAAACAT
TATTTTTCCGCCGCTGGAAGGCCAAATGGCCGGTGAAAATGTGCTGTATGCCATGGGCGGCTTCCTACTGACCGCAGTCG
GTCTGCCGCTGGTTTCCATTATCGCCGTCGCCAGCGCCGGCGGCGGCCTGCCGGCGATGACCCGCGATTTACCGCGCGGT
ACTGGCACTTTTGCCGCCGTGCTGCTGTTTATCATCATCGGGCCGGCCTTTGCCGCGCCGCGTACCGGTCTGGTAGCCTT
TGAAATGGCGATCCGTCCGCTGCTGGGCAGTCAGGGCGAAAGCGCACTGCAGGTAGGTGCCGTGACCCTGGACGGGGTAC
AGATGGCCGTTACGGCGCTGTTCTTTGTCGTAACGCTGGCGCTGGCCTGGAGCCGCGGCAAGCTGATCGACAGCGTGGGC
AAGTTGTTGACGCCGATGTTGTTCCTGCTGCTGGTCGTGCTGGCGCTCGGGGTGCTGCTGCGCCCGCAGGGTGAGATCGT
TGCTCCGGTCGAGGCCTATCAGACCATGGCCTTCAGCAAGGGCTTTATCGGTGGCTATAACACCATGGATACCTTCGCCG
CGCTGATGTTTGGCATGCTGCTGGTGGACGTGCTGCGCGGTAAAGGGGTCACTGCGCCGCGCGCCTGCTTCCGCTATCTG
AGCATTGCCGGAGTGATCGCGGCTATCGGTCTGGCTTTTGTCTATGTTTCCCTGTTTTGGCTGGGGGCGACCTCGTCTGG
CGTTGCTCCGGGTGCGAGCAACGGTGGGGTGGTGCTCGCGGCCTATGTACAGGCGCTGTTCGGACAGCCGGGGCAGTGGA
TCCTGTCACTGGTGGTGCTGCTGGCCTGTCTGACCACGGCGGTGGGGTTGGTGAGCGCCTGCGCCGACTACTTCTCAACC
CTGACGCCGCTGCGCTACCGTCAGTGGGTAGTGTTGCTGTGTGCGATCTGTGCAGTGGTGGCCAACGTGGGATTAAGTCG
TTTGATTGCCCTGTCGGTGCCGGTGCTGATGGCGCTCTATCCGCTGGCTATCGCTTTGGTGGTGCTGACCTTCATCCGTC
GCTGGCTACGTAACCCGGTAGTGGGCTACCGCTTGGTGATGCTGGTTGCGCTGTTGTGCAGCCTGATGGATGGCGCCCGC
GCCGCCGGCGCCGAGAGCGGTTGGCTGGGCGAGCTGTTGGCATGGCAGATGCGTACCATGCCTCTGGCCGCTGATGGTAT
GGCCTGGGTACTGCCGTCTACGCTGGCGCTGCTGGTTGCGCTGCTGTTGCCGGCGGGAGCCCGCAACGCCGGCGCGCCGC
AGGCCGCCTGA

Upstream 100 bases:

>100_bases
CATACCTGAGTTTACTTCTGGACAGACAGCATTTTAATGCGGTACCCCCGTGCGTTTCTGCGGCGTAGACGGTTGGGTAA
GCGAGATAAGAGGGCAGAAT

Downstream 100 bases:

>100_bases
GTCAGGTCGCTGTTTCTGGAAAACCGTCGGGCGCCTCAAGGGCGTCCGATTTTTTTGTCTCTTGTCCCCCGTCTATGCCC
TGAATTTTGTTGCCGCCGCG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MQQRLSMADTLGLGLMTFAFFLGAGNIIFPPLEGQMAGENVLYAMGGFLLTAVGLPLVSIIAVASAGGGLPAMTRDLPRG
TGTFAAVLLFIIIGPAFAAPRTGLVAFEMAIRPLLGSQGESALQVGAVTLDGVQMAVTALFFVVTLALAWSRGKLIDSVG
KLLTPMLFLLLVVLALGVLLRPQGEIVAPVEAYQTMAFSKGFIGGYNTMDTFAALMFGMLLVDVLRGKGVTAPRACFRYL
SIAGVIAAIGLAFVYVSLFWLGATSSGVAPGASNGGVVLAAYVQALFGQPGQWILSLVVLLACLTTAVGLVSACADYFST
LTPLRYRQWVVLLCAICAVVANVGLSRLIALSVPVLMALYPLAIALVVLTFIRRWLRNPVVGYRLVMLVALLCSLMDGAR
AAGAESGWLGELLAWQMRTMPLAADGMAWVLPSTLALLVALLLPAGARNAGAPQAA

Sequences:

>Translated_456_residues
MQQRLSMADTLGLGLMTFAFFLGAGNIIFPPLEGQMAGENVLYAMGGFLLTAVGLPLVSIIAVASAGGGLPAMTRDLPRG
TGTFAAVLLFIIIGPAFAAPRTGLVAFEMAIRPLLGSQGESALQVGAVTLDGVQMAVTALFFVVTLALAWSRGKLIDSVG
KLLTPMLFLLLVVLALGVLLRPQGEIVAPVEAYQTMAFSKGFIGGYNTMDTFAALMFGMLLVDVLRGKGVTAPRACFRYL
SIAGVIAAIGLAFVYVSLFWLGATSSGVAPGASNGGVVLAAYVQALFGQPGQWILSLVVLLACLTTAVGLVSACADYFST
LTPLRYRQWVVLLCAICAVVANVGLSRLIALSVPVLMALYPLAIALVVLTFIRRWLRNPVVGYRLVMLVALLCSLMDGAR
AAGAESGWLGELLAWQMRTMPLAADGMAWVLPSTLALLVALLLPAGARNAGAPQAA
>Mature_456_residues
MQQRLSMADTLGLGLMTFAFFLGAGNIIFPPLEGQMAGENVLYAMGGFLLTAVGLPLVSIIAVASAGGGLPAMTRDLPRG
TGTFAAVLLFIIIGPAFAAPRTGLVAFEMAIRPLLGSQGESALQVGAVTLDGVQMAVTALFFVVTLALAWSRGKLIDSVG
KLLTPMLFLLLVVLALGVLLRPQGEIVAPVEAYQTMAFSKGFIGGYNTMDTFAALMFGMLLVDVLRGKGVTAPRACFRYL
SIAGVIAAIGLAFVYVSLFWLGATSSGVAPGASNGGVVLAAYVQALFGQPGQWILSLVVLLACLTTAVGLVSACADYFST
LTPLRYRQWVVLLCAICAVVANVGLSRLIALSVPVLMALYPLAIALVVLTFIRRWLRNPVVGYRLVMLVALLCSLMDGAR
AAGAESGWLGELLAWQMRTMPLAADGMAWVLPSTLALLVALLLPAGARNAGAPQAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=442, Percent_Identity=41.6289592760181, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47527; Mature: 47527

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRLSMADTLGLGLMTFAFFLGAGNIIFPPLEGQMAGENVLYAMGGFLLTAVGLPLVSI
CCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAVASAGGGLPAMTRDLPRGTGTFAAVLLFIIIGPAFAAPRTGLVAFEMAIRPLLGSQGE
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
SALQVGAVTLDGVQMAVTALFFVVTLALAWSRGKLIDSVGKLLTPMLFLLLVVLALGVLL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPQGEIVAPVEAYQTMAFSKGFIGGYNTMDTFAALMFGMLLVDVLRGKGVTAPRACFRYL
CCCCCEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIAGVIAAIGLAFVYVSLFWLGATSSGVAPGASNGGVVLAAYVQALFGQPGQWILSLVVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LACLTTAVGLVSACADYFSTLTPLRYRQWVVLLCAICAVVANVGLSRLIALSVPVLMALY
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLAIALVVLTFIRRWLRNPVVGYRLVMLVALLCSLMDGARAAGAESGWLGELLAWQMRTM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
PLAADGMAWVLPSTLALLVALLLPAGARNAGAPQAA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQRLSMADTLGLGLMTFAFFLGAGNIIFPPLEGQMAGENVLYAMGGFLLTAVGLPLVSI
CCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAVASAGGGLPAMTRDLPRGTGTFAAVLLFIIIGPAFAAPRTGLVAFEMAIRPLLGSQGE
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
SALQVGAVTLDGVQMAVTALFFVVTLALAWSRGKLIDSVGKLLTPMLFLLLVVLALGVLL
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
RPQGEIVAPVEAYQTMAFSKGFIGGYNTMDTFAALMFGMLLVDVLRGKGVTAPRACFRYL
CCCCCEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SIAGVIAAIGLAFVYVSLFWLGATSSGVAPGASNGGVVLAAYVQALFGQPGQWILSLVVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHH
LACLTTAVGLVSACADYFSTLTPLRYRQWVVLLCAICAVVANVGLSRLIALSVPVLMALY
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLAIALVVLTFIRRWLRNPVVGYRLVMLVALLCSLMDGARAAGAESGWLGELLAWQMRTM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
PLAADGMAWVLPSTLALLVALLLPAGARNAGAPQAA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]