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The map label for this gene is eno [H]

Identifier: 238920895

GI number: 238920895

Start: 2907723

End: 2909024

Strand: Reverse

Name: eno [H]

Synonym: NT01EI_3022

Alternate gene names: 238920895

Gene position: 2909024-2907723 (Counterclockwise)

Preceding gene: 238920896

Following gene: 238920887

Centisome position: 76.31

GC content: 55.15

Gene sequence:

>1302_bases
ATGTCCAAAATCGTTAAAGTTATCGGTCGCGAAATCATCGACTCCCGCGGTAACCCGACTGTAGAAGCCGAAGTTCATCT
GGAAGGCGGTTTCGTCGGTATGGCTGCTGCGCCGTCAGGTGCTTCTACCGGTTCCCGCGAAGCGCTGGAGTTGCGCGACG
GTGACAAGTCACGTTTCCTGGGTAAAGGCGTTCTGAAAGCGGTTTCCGCCGTTAATGGCCCGATCGCTGAAGTCATCATC
GGTAAAGACGCCAAAGATCAGGCAAACATCGACCAGATCATGATTGAGCTGGACGGCACTGAAAACAAATCCAAGTTTGG
TGCCAACGCCATTCTGGCGGTTTCCCTGGCCAACGCTAAAGCAGCCGCTGCCGCTAAAGGCATGCCGCTGTACGCTCACA
TCGCCGAGCTGAACGGTACTCCGGGCAAATACTCCATGCCGCTGCCGATGATGAACATCATCAACGGCGGTGAGCACGCC
GACAACAACGTCGACATCCAGGAGTTTATGATTCAGCCGGTTGGCGCTAAGACCCTGAAAGAAGCGGTACGCATCGGTTC
TGAAGTGTTCCACAACCTGGCTAAGGTGCTGAAGTCTAAAGGCATGAACACCGCTGTGGGCGACGAAGGCGGCTACGCGC
CGAACCTGGAGTCCAACGCGGCAGCGCTGGCGGCCATCAAGGAAGCCGTTGAGAAAGCCGGTTACGTGCTGGGTAAAGAC
GTTACCCTGGCGATGGACTGCGCCGCCTCTGAGTTCTACAACAAAGAAACCGGTATGTATGAACTGAAAGGCGAAGGCAA
ATCCTTCACCTCCAACGAGTTCACCCACTACCTGGAAGGTCTGACCAAAGAGTACCCGATCGTTTCTATCGAAGACGGTC
TGGACGAGTCTGACTGGGATGGCTTCGCTTATCAGACCAAAGTGATGGGTGGCAAGCTGCAGCTGGTGGGTGACGATCTG
TTCGTGACCAACACCAAGATCCTGAAAGAGGGCATCGAGAAAGGCATCGCTAACTCCATCCTGATCAAGTTCAACCAGAT
CGGTTCTCTGACCGAAACCCTGGCTGCTATCAAGATGGCGAAAGACGCTGGCTACACCGCCGTTATCTCTCACCGTTCAG
GTGAAACCGAAGATGCGACCATCGCCGATCTGGCGGTAGGTACCGCGGCTGGCCAGATCAAGACCGGTTCCATGAGCCGT
TCTGATCGCGTAGCCAAGTACAACCAGCTGATCCGTATCGAAGAAGCGCTGGGCGCGGCTGCACCGTTCAACGGTCTGAA
AGAAGTGAAAGGCCAGCATTAA

Upstream 100 bases:

>100_bases
AGAAACGTCAGGTGAAATAAGTGATTATGGGCAGGCGCGGGACTTGGGTTCCGCGTCTGTCGTCTGGAGTTTTAGTTTAA
CTTGTATCGAGGAAAACCTA

Downstream 100 bases:

>100_bases
TCTGCCGCTTTTCTGATGTAAAAAACCCGCCATCCGGCGGGTTTTTTATGTCCGTGGTGTAACGGTTAACTGATCGCCTC
TTCAGTGAGCAGGATTTGAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNGPIAEVII
GKDAKDQANIDQIMIELDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYAHIAELNGTPGKYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRIGSEVFHNLAKVLKSKGMNTAVGDEGGYAPNLESNAAALAAIKEAVEKAGYVLGKD
VTLAMDCAASEFYNKETGMYELKGEGKSFTSNEFTHYLEGLTKEYPIVSIEDGLDESDWDGFAYQTKVMGGKLQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGAAAPFNGLKEVKGQH

Sequences:

>Translated_433_residues
MSKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNGPIAEVII
GKDAKDQANIDQIMIELDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYAHIAELNGTPGKYSMPLPMMNIINGGEHA
DNNVDIQEFMIQPVGAKTLKEAVRIGSEVFHNLAKVLKSKGMNTAVGDEGGYAPNLESNAAALAAIKEAVEKAGYVLGKD
VTLAMDCAASEFYNKETGMYELKGEGKSFTSNEFTHYLEGLTKEYPIVSIEDGLDESDWDGFAYQTKVMGGKLQLVGDDL
FVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSR
SDRVAKYNQLIRIEEALGAAAPFNGLKEVKGQH
>Mature_432_residues
SKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFLGKGVLKAVSAVNGPIAEVIIG
KDAKDQANIDQIMIELDGTENKSKFGANAILAVSLANAKAAAAAKGMPLYAHIAELNGTPGKYSMPLPMMNIINGGEHAD
NNVDIQEFMIQPVGAKTLKEAVRIGSEVFHNLAKVLKSKGMNTAVGDEGGYAPNLESNAAALAAIKEAVEKAGYVLGKDV
TLAMDCAASEFYNKETGMYELKGEGKSFTSNEFTHYLEGLTKEYPIVSIEDGLDESDWDGFAYQTKVMGGKLQLVGDDLF
VTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRS
DRVAKYNQLIRIEEALGAAAPFNGLKEVKGQH

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.3642691415313, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.3642691415313, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=51.7401392111369, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.508120649652, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.4848484848485, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789141, Length=433, Percent_Identity=89.6073903002309, Blast_Score=760, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=53.5796766743649, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=53.5796766743649, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=48.4375, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.8845265588915, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=47.1264367816092, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=47.1264367816092, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=47.1264367816092, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=48.9607390300231, Blast_Score=353, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=51.0250569476082, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=51.0250569476082, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=51.0250569476082, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=51.0250569476082, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=426, Percent_Identity=51.8779342723005, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=426, Percent_Identity=51.8779342723005, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45747; Mature: 45615

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFL
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVSAVNGPIAEVIIGKDAKDQANIDQIMIELDGTENKSKFGANAILAVSLANAK
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCH
AAAAAKGMPLYAHIAELNGTPGKYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCCCEEEEEECCCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRIGSEVFHNLAKVLKSKGMNTAVGDEGGYAPNLESNAAALAAIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCAASEFYNKETGMYELKGEGKSFTSNEFTHYLEGLTKEYPIVSIEDGLDESDWD
EEEEEHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFAYQTKVMGGKLQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEEEECCCEEEEEECCEEECHHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGAA
HCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQH
CCCCCHHHHCCCC
>Mature Secondary Structure 
SKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTGSREALELRDGDKSRFL
HHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVSAVNGPIAEVIIGKDAKDQANIDQIMIELDGTENKSKFGANAILAVSLANAK
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCH
AAAAAKGMPLYAHIAELNGTPGKYSMPLPMMNIINGGEHADNNVDIQEFMIQPVGAKTLK
HHHHHCCCCCEEEEEECCCCCCCEECCCCHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EAVRIGSEVFHNLAKVLKSKGMNTAVGDEGGYAPNLESNAAALAAIKEAVEKAGYVLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLAMDCAASEFYNKETGMYELKGEGKSFTSNEFTHYLEGLTKEYPIVSIEDGLDESDWD
EEEEEHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCC
GFAYQTKVMGGKLQLVGDDLFVTNTKILKEGIEKGIANSILIKFNQIGSLTETLAAIKMA
CEEEEEEECCCEEEEEECCEEECHHHHHHHHHHHCCCCCEEEEEHHHCHHHHHHHHHHHH
KDAGYTAVISHRSGETEDATIADLAVGTAAGQIKTGSMSRSDRVAKYNQLIRIEEALGAA
HCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APFNGLKEVKGQH
CCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]